{"title":"Measuring the phase and intensity of the light in microscopy for biological sample characterization at the nanoscale","authors":"P. Bon","doi":"10.1364/DH.2019.TH1A.1","DOIUrl":"https://doi.org/10.1364/DH.2019.TH1A.1","url":null,"abstract":"Conventional optical imaging technique are only sensitive to the light intensity. However, many other optical parameters can be probed to enhance the quantity of information retrieved from a biological sample. I will discuss in this presentation how and why measuring the so-called phase of the light. Applications both in the scope of label-free microscopy and for 3D fluorescence super-resolution will be discussed. In particular, I will show that this quantitative imaging modality allows to identify without labeling many organelles at high frame-rate and for any duration1 in living cells. Then I will move to the application of imaging both the intensity and the phase of fluorescent single emitter and demonstrate that it leads to single molecule 3D localization and 3D super-resolution even at depth in biological tissues2","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133602082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of Wollaston prism for dual-reference digital holographic interferometry","authors":"J. Desse, F. Olchewsky","doi":"10.1364/DH.2019.TU4B.4","DOIUrl":"https://doi.org/10.1364/DH.2019.TU4B.4","url":null,"abstract":"A Wollaston prism is used in digital holographic interferometer developed for analyzing high density gradients encountered in transonic and supersonic flows. Inserted in the reference arm of the interferometer, the Wollaston prism allows generating two orthogonally crossed waves and analyzing shocks waves whatever their orientation.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130240946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Hologram Windowing on Correlation of 3D Objects","authors":"Haowen Zhou, Runzi Hou, B. Bordbar, P. Banerjee","doi":"10.1364/DH.2019.TH2B.8","DOIUrl":"https://doi.org/10.1364/DH.2019.TH2B.8","url":null,"abstract":"Holograms are recorded, windowed, cropped and correlated. Correlation peak values are extracted as a metric to evaluate correlation of 3D surface features. The effect of hologram windowing on correlation of 3D real objects are investigated.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115424378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Single-shot fluorescence quantitative phase microscopy using Transport of Intensity Equation","authors":"P. K. Poola, V. Pandiyan, S. Rath, R. John","doi":"10.1364/DH.2019.M5B.5","DOIUrl":"https://doi.org/10.1364/DH.2019.M5B.5","url":null,"abstract":"In this work, we report retrieval of 3-D phase of homogenously fluorescent objects with a constant absorption coefficient that is homogenous. TIE equations can be used to retrieve the 3-D phase.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115705132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaping Zhang, Wang Rende, Lin Wang, P. Tsang, T. Poon
{"title":"Annular pupil in optical scanning holography","authors":"Yaping Zhang, Wang Rende, Lin Wang, P. Tsang, T. Poon","doi":"10.1364/DH.2019.W1A.5","DOIUrl":"https://doi.org/10.1364/DH.2019.W1A.5","url":null,"abstract":"Optical scanning holography (OSH) is a variant of two-pupil heterodyning image processing. We investigate the use of one pupil as a delta function and the other being an annulus as an extended application of OSH.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114776579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhang Cheng, Xun Haitao, Wang Meiqin, Chen Qianwen
{"title":"Compressive Optical Encryption of Three-dimensional Image","authors":"Zhang Cheng, Xun Haitao, Wang Meiqin, Chen Qianwen","doi":"10.1364/DH.2019.W3A.19","DOIUrl":"https://doi.org/10.1364/DH.2019.W3A.19","url":null,"abstract":"A new compressive optical encryption of three-dimensional image method is presented. The 3D object can be encrypted into a hologram and recovered via phase-shifting first and compressive Fresnel holography. The feasibility and superiority are verified.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116029030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Buitrago-Duque, R. Castaneda, J. Garcia-Sucerquia
{"title":"Denoising Phase Maps of Digital Holographic Microscopy by Complex Tuning","authors":"C. Buitrago-Duque, R. Castaneda, J. Garcia-Sucerquia","doi":"10.1364/DH.2019.TH3A.15","DOIUrl":"https://doi.org/10.1364/DH.2019.TH3A.15","url":null,"abstract":"Noisy phase maps are numerically compensated by their understanding as a complex vector superposition of the object and the noise. Denoising by estimating the noise vector achieves PSNR increase from 2 dB to 22 dB.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120945299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Calculating Real-time Computer-Generated Holograms for Holographic 3D Displays through Deep Learning","authors":"Sheng-Chi Liu, Jin Li, D. Chu","doi":"10.1364/DH.2019.TU4A.7","DOIUrl":"https://doi.org/10.1364/DH.2019.TU4A.7","url":null,"abstract":"A deep learning method is proposed to calculate holograms in real-time. After training, it can generate holograms for all R/G/B channels within 10 msec. Simulation results confirm successfully reconstruct the target training and testing images.","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115160211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Petrov, A. Belashov, S. S. Nalegaev, I. Shevkunov, S. Putilin, Yu Chih Lin, Chau-Jern Cheng
{"title":"Local Optical Nonlinear Responses in Time-Resolved Inline Digital Holographic Measurements","authors":"N. Petrov, A. Belashov, S. S. Nalegaev, I. Shevkunov, S. Putilin, Yu Chih Lin, Chau-Jern Cheng","doi":"10.1364/DH.2019.W1A.7","DOIUrl":"https://doi.org/10.1364/DH.2019.W1A.7","url":null,"abstract":"","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"155 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132511336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coherent x-ray imaging at the nanoscale: propagating from cells to tissues","authors":"C. Jacobsen, Ming Du, Sajid Ali, S. Kandel","doi":"10.1364/DH.2019.TH2B.5","DOIUrl":"https://doi.org/10.1364/DH.2019.TH2B.5","url":null,"abstract":"","PeriodicalId":448778,"journal":{"name":"Digital Holography and Three-Dimensional Imaging 2019","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123013004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}