Revista da Associação Médica Brasileira最新文献_第5页

Human papillomavirus DNA testing with the urine sample is not yet available: the accuracy of two distinct kits 尿样人类乳头瘤病毒 DNA 检测尚未推出：两种不同检测试剂盒的准确性

Revista da Associação Médica Brasileira Pub Date : 2024-01-05 DOI: 10.1590/1806-9282.20230668

F. Kazancı, Ozlem Erdem, M. Onan, G. Bozdayi

{"title":"Human papillomavirus DNA testing with the urine sample is not yet available: the accuracy of two distinct kits","authors":"F. Kazancı, Ozlem Erdem, M. Onan, G. Bozdayi","doi":"10.1590/1806-9282.20230668","DOIUrl":"https://doi.org/10.1590/1806-9282.20230668","url":null,"abstract":"SUMMARY OBJECTIVE: The aim of this study was to assess the results and efficiency of two real-time polymerase chain reaction procedures for detecting human papillomavirus utilizing urine samples. METHODS: This study comprised 151 patients who had previously tested positive for human papillomavirus in their cervical samples. Two different commercial real-time polymerase chain reaction techniques were used for identification and genotyping human papillomavirus in urine specimens. The urine samples of 151 patients were evaluated via the Roche Cobas test, and the urine samples of 91 patients were also evaluated via the Qiagen test. RESULTS: The overall consistency of urine and cervical swab specimens for the identification of human papillomavirus in Roche Cobas and Qiagen tests were 44.8 and 44%, respectively. The rates of positive human papillomavirus results from urine samples were 57 and 70.3%, respectively. The overall concordance among Roche Cobas and Qiagen tests utilizing urine samples for human papillomavirus type 16/18 was 84.3% with a kappa value of 0.675, and for other high-risk-human papillomavirus, it was 75.60% with a kappa value of 0.535. Roche Cobas showed high concordance with Qiagen test. CONCLUSION: human papillomavirus positivity was not detected in all urine samples. It is still inappropriate to recommend the use of urine liquid biopsy for the accurate and reliable detection of human papillomavirus. Due to the lack of a standardized tool, the utilization of urine samples as a screening human papillomavirus test remains a challenge.","PeriodicalId":447105,"journal":{"name":"Revista da Associação Médica Brasileira","volume":"1 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caregiver burden, family adaptation, partnership, growth, affection, and resolve, anxiety levels, and perceived social support in relatives of patients with open heart surgery 开放式心脏手术患者亲属的护理负担、家庭适应、伙伴关系、成长、亲情和决心、焦虑水平以及感知到的社会支持

Revista da Associação Médica Brasileira Pub Date : 2024-01-05 DOI: 10.1590/1806-9282.20230494

N. Dolapoğlu, A. Dolapoğlu, Sinan Altunöz

{"title":"Caregiver burden, family adaptation, partnership, growth, affection, and resolve, anxiety levels, and perceived social support in relatives of patients with open heart surgery","authors":"N. Dolapoğlu, A. Dolapoğlu, Sinan Altunöz","doi":"10.1590/1806-9282.20230494","DOIUrl":"https://doi.org/10.1590/1806-9282.20230494","url":null,"abstract":"SUMMARY OBJECTIVE: The aim of this study was to examine the relationship between caregiver burden, family adaptation, partnership, growth, affection, and resolve score, anxiety levels, and the perceived social support of the relatives of patients who had open heart surgery. METHODS: Volunteers among the relatives of patients who had open heart surgery in our cardiovascular surgery clinic and were followed up in the first 3 months were included in the study. The cardiovascular surgeons recorded the sociodemographic data of the relatives of the patients and directed them to a psychiatry clinic for further evaluation. The caregiver burden scale, family adaptation, partnership, growth, affection, and resolve scale, anxiety level scale, and perceived social support scale were applied to the relatives of the patients who participated in the study. RESULTS: Within the scope of the study, a total of 51 individuals, 29.4% (n=15) men and 70.6% (n=36) women, were included in the evaluation. The participants’ ages ranged from 32 to 68 years, with an average age of 48 years. There was a statistically significant relationship between the caregiving burden scale score and the scale scores other than age (p<0.05). There was a statistically significant difference in terms of caregiving burden scale score, working status, physical and psychological problems, changes in home life, and changes in family relationships (p<0.05). CONCLUSION: The fact that the need for security and intimacy is related to anxiety and depression can be interpreted as the caregiving problems of the relatives of the patients who think that their patients are safe and feel closer to the intensive care personnel will decrease. Their depression and anxiety levels will also decrease.","PeriodicalId":447105,"journal":{"name":"Revista da Associação Médica Brasileira","volume":"12 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of early neonatal sepsis and positive maternal culture for group B beta-hemolytic Streptococcus 新生儿早期败血症和母体 B 组β-溶血性链球菌培养阳性的发病率

Revista da Associação Médica Brasileira Pub Date : 2024-01-05 DOI: 10.1590/1806-9282.20230021

Cecília Gomes Cunha Silva, Maria Júlia Arantes Leobas, Andressa Paes Medeiros de Freitas, Júlia Teoro Mansano, Jaider Antonio Vidigal Rodrigues, E. Araujo Júnior, A. Peixoto

{"title":"Prevalence of early neonatal sepsis and positive maternal culture for group B beta-hemolytic Streptococcus","authors":"Cecília Gomes Cunha Silva, Maria Júlia Arantes Leobas, Andressa Paes Medeiros de Freitas, Júlia Teoro Mansano, Jaider Antonio Vidigal Rodrigues, E. Araujo Júnior, A. Peixoto","doi":"10.1590/1806-9282.20230021","DOIUrl":"https://doi.org/10.1590/1806-9282.20230021","url":null,"abstract":"SUMMARY OBJECTIVE: The aim of this study was to evaluate the prevalence of early neonatal sepsis in pregnant women with a positive culture for group B beta-hemolytic Streptococcus in a middle-income city in Southeastern Brazil. METHODS: A retrospective cohort study was conducted, involving singleton low- and high-risk pregnancies in whom group B beta-hemolytic Streptococcus cultures were evaluated between 35 and 37 weeks of gestation using vaginal and anal swabs. A specific medium (Todd-Hewitt) was used for culturing. The pregnant women were divided into two groups based on positive (n==201) and negative (n==420) cultures for group B beta-hemolytic Streptococcus. RESULTS: The maternal colonization rate by group B beta-hemolytic Streptococcus was 32.3%. The prevalence of early neonatal sepsis was 1.0% (2/201) among patients with a positive group B beta-hemolytic Streptococcus culture and 1.9% (8/420) among patients with a negative culture. Among the patients who underwent adequate prophylaxis, crystalline penicillin G was used in 51.9% (54/104), followed by cefazolin in 43.3% (45/104), ampicillin in 3.8% (4/104), and clindamycin in 1.0% (1/104). A model that included prematurity (p==0.001) proved to be an independent risk predictor of early neonatal sepsis [χ2 (1)==15.0, odds ratio: 16.9, 95% confidence interval: 4.7–61.6, p<0.001, Nagelkerke R2==0.157]. CONCLUSION: The prevalence of a positive culture for group B beta-hemolytic Streptococcus was high. However, the prevalence of early neonatal sepsis was low in pregnant women with both positive and negative group B beta-hemolytic Streptococcus cultures and in pregnant women with a positive culture who underwent both adequate and inadequate antibiotic prophylaxis. Prematurity proved to be an independent predictor of early neonatal sepsis, considering the entire study population.","PeriodicalId":447105,"journal":{"name":"Revista da Associação Médica Brasileira","volume":"38 27","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral probiotics and vaginal microbiome in post-menopause women: an opinion for the improvement of natural therapies in gynecology 口服益生菌与绝经后妇女的阴道微生物群：改进妇科自然疗法的观点

Revista da Associação Médica Brasileira Pub Date : 2024-01-05 DOI: 10.1590/1806-9282.702EDIT

Virginia Franco Silva, Paulo Refinetti, Franco Vicariotto, E. Baracat, José Maria Soares Júnior

{"title":"Oral probiotics and vaginal microbiome in post-menopause women: an opinion for the improvement of natural therapies in gynecology","authors":"Virginia Franco Silva, Paulo Refinetti, Franco Vicariotto, E. Baracat, José Maria Soares Júnior","doi":"10.1590/1806-9282.702EDIT","DOIUrl":"https://doi.org/10.1590/1806-9282.702EDIT","url":null,"abstract":"The vaginal microbiota represents an intricate and multifaceted biome that directly modulates female health. It refers to the community of microorganisms, including bacteria, fungi, and viruses, which reside in the vagina 1 . This complex ecosystem plays a crucial role in female health and a potential role in reproductive fitness. The vaginal microbiome’s composition varies among individuals and can be influenced by numerous factors such as age, racial factors, hormonal changes, sexual activity, and the use of contra-ceptives or antibiotics. In many healthy individuals, the vaginal microbiome is often dominated by bacteria from the Lactobacillus genus. These bacteria produce lactic acid, helping to maintain a low pH in the vagina and thus providing a natural defense against the colonization of pathogenic microbes. However, an imbalance or dysbiosis in the vaginal microbiome can lead to various health issues such as bacterial vaginosis, yeast infections, or increased susceptibility to sexually transmitted infections 2 . Disruptions in this microbiome, particularly during the menopausal transition, are caused especially by the lack of estrogen and can lead to an array of deleterious conditions such as atrophic vaginitis, recurrent urinary tract","PeriodicalId":447105,"journal":{"name":"Revista da Associação Médica Brasileira","volume":"1 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant effect of Rosa pimpinellifolia L. fruit extract on cholestatic liver injury: an experimental study 蔷薇果提取物对胆汁淤积性肝损伤的抗氧化作用：一项实验研究

Revista da Associação Médica Brasileira Pub Date : 2024-01-05 DOI: 10.1590/1806-9282.20230720

Mahmut Kaan Demircioglu, Z. Demircioglu, Ozlem Cakir, K. Yanar, M. Ozguven, Pinar Atukeren, O. B. Gulcicek, Bulent Citgez, Pinar Yazici

{"title":"Antioxidant effect of Rosa pimpinellifolia L. fruit extract on cholestatic liver injury: an experimental study","authors":"Mahmut Kaan Demircioglu, Z. Demircioglu, Ozlem Cakir, K. Yanar, M. Ozguven, Pinar Atukeren, O. B. Gulcicek, Bulent Citgez, Pinar Yazici","doi":"10.1590/1806-9282.20230720","DOIUrl":"https://doi.org/10.1590/1806-9282.20230720","url":null,"abstract":"SUMMARY BACKGROUND: Antioxidants have been considered a rational curative strategy to prevent and cure liver diseases involving oxidative stress. An acute obstructive jaundice rat model was established to investigate the in vivo hepatoprotective efficacy of Rosa pimpinellifolia L. METHODS: The experimental jaundice model was performed by binding the main bile duct in 25 male Sprague-Dawley rats. All rats were randomly divided into five groups: first group: laparotomy-sham-only, second group: biliary tract binding (control), and third, fourth, and fifth groups: treatment groups with 250, 500, and 750 mg/kg fruit extracts daily, respectively. RESULTS: Considering dosage, although there was no significant therapeutic effect in the 250 mg/kg of Rosa pimpinellifolia L. group, the best results were found in the 500 mg/kg dose group, while results in the 750 mg/kg dose group showed consistent correlation with proinflammatory response. With regard to biochemical parameters, lipid hydroperoxide level in the rat serum and liver tissue was significantly decreased in all treatment groups. Amadori products, which are one of the early markers of glycol-oxidative stress, showed statistical significance in the treatment. CONCLUSION: It was revealed that the antioxidant effect of Rosa pimpinellifolia L. was more prominent in the early stages of hepatic injury secondary to oxidative stress.","PeriodicalId":447105,"journal":{"name":"Revista da Associação Médica Brasileira","volume":"14 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0