Journal of Regenerative Medicine and Biology Research最新文献

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The Application of Transgenesis to Abraham Trembley’s Hydra, an Ancient Animal Model for the Study of Regeneration that has now entered the 21st Century 亚伯拉罕·特伦布利的九头蛇，一个古老的动物模型再生研究，现在已经进入21世纪的应用转基因

Journal of Regenerative Medicine and Biology Research Pub Date : 1900-01-01 DOI: 10.46889/jrmbr.2020.1101

M. P. Sarras Jr.

引用次数: 0

The Future Role of Mesenchymal Stem Cells in Tissue Repair and Medical Therapeutics: Realities and Expectations 间充质干细胞在组织修复和医学治疗中的未来作用:现实和期望

Journal of Regenerative Medicine and Biology Research Pub Date : 1900-01-01 DOI: 10.46889/jrmbr.2020.1201

K. Al-Anazi

{"title":"The Future Role of Mesenchymal Stem Cells in Tissue Repair and Medical Therapeutics: Realities and Expectations","authors":"K. Al-Anazi","doi":"10.46889/jrmbr.2020.1201","DOIUrl":"https://doi.org/10.46889/jrmbr.2020.1201","url":null,"abstract":"Mesenchymal Stem Cells (MSCs); which were first described by Alexander Fridenstein in the 1960s; are heterogeneous, non-hematopoietic, adult multipotent stromal progenitor cells that are capable of self-renewal and differentiation into various cell types [1-8]. They can be isolated from various sources including: Bone Marrow (BM) which is the main source, peripheral blood, umbilical cord blood, amniotic fluid, placenta, Adipose Tissue (AT), dental pulp, synovial fluid, salivary glands, liver, lung, skin and skeletal muscles [1-10]. MSCs have the following distinguishing features: adherence to the plastic vessel; capacity to different into osteoblasts, adipocytes and chondrocytes; and being characteristically positive for CD105, CD73, and CD90 and characteristically negative for CD45, CD34, CD11b, CD14, CD19, CD79a, and human leukocyte antigen (HLA)-DR on flow cytometry [1,3,4,11-16]. However, under certain circumstances, MSCs obtained from BM, AT, and other sources may express CD34 surface markers [5-8,17]. Additionally, MSCs do not express significant histocompatibility complexes and immune stimulating molecules. Consequently, they escape immune surveillance and their clinical utilization in transplantation is not associated with graft rejection [10].","PeriodicalId":447063,"journal":{"name":"Journal of Regenerative Medicine and Biology Research","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125198878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local Anesthetics Can Affect the Efficacy of Telomerase-Positive Stem Cells 局部麻醉剂可影响端粒酶阳性干细胞的疗效

Journal of Regenerative Medicine and Biology Research Pub Date : 1900-01-01 DOI: 10.46889/jrmbr.2020.1102

H. Young, M. Speight

{"title":"Local Anesthetics Can Affect the Efficacy of Telomerase-Positive Stem Cells","authors":"H. Young, M. Speight","doi":"10.46889/jrmbr.2020.1102","DOIUrl":"https://doi.org/10.46889/jrmbr.2020.1102","url":null,"abstract":"Early clinical studies with telomerase-positive stem cells demonstrated no response when these stem cells were mixed with lidocaine prior to clinical treatments in multiple individuals. Their stem cells demonstrated a positive response when mixed with normal sterile saline utilizing alternative routes of implantation. We hypothesized that lidocaine killed the stem cells before injection and that dead stem cells would give no response. We tested five local anesthetics, e.g., bupivacaine, lidocaine, marcaine, novocaine and procaine, with sterile saline in a series of blinded experiments to determine their ability to affect the viability of telomerase-positive stem cells. A mixture of TSCs, PSCs and MesoSCs were utilized from five individuals, three males and two females. Trypan blue was used to distinguish live versus dead PSCs and MesoSCs. The number of dead cells divided by total number of cells and multiplied by 100 was used for each test solution to determine their respective % kill ratio. Sample size was n=180 for each test solution. Lidocaine demonstrated a 100% kill ratio; novocaine and procaine demonstrated a 50% kill ratio and marcaine, bupivacaine and sterile saline demonstrated a 0% kill ratio. The","PeriodicalId":447063,"journal":{"name":"Journal of Regenerative Medicine and Biology Research","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126967935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2