Biomedical Spectroscopy and Imaging最新文献_第5页

Methodology for 3D image reconstruction of the female pelvis from upright open MRI (MRO) 2D imaging 从直立开放MRI (MRO)二维成像中重建女性骨盆三维图像的方法

Biomedical Spectroscopy and Imaging Pub Date : 2018-01-01 DOI: 10.3233/BSI-180178

Marwa Abdulaziz, L. Stothers, A. Macnab

引用次数: 2

Vacuum-ultraviolet circular dichroism study of oligosaccharides using a synchrotron-radiation spectrophotometer 用同步辐射分光光度计研究低聚糖的真空-紫外圆二色性

Biomedical Spectroscopy and Imaging Pub Date : 2017-12-27 DOI: 10.3233/BSI-170169

K. Matsuo

引用次数: 3

Monomeric green fluorescent protein as a protein standard for small angle scattering 单体绿色荧光蛋白作为小角度散射的蛋白质标准

Biomedical Spectroscopy and Imaging Pub Date : 2017-12-27 DOI: 10.3233/BSI-170167

Daniel P Myatt, L. Hatter, Sarah E Rogers, A. Terry, L. Clifton

{"title":"Monomeric green fluorescent protein as a protein standard for small angle scattering","authors":"Daniel P Myatt, L. Hatter, Sarah E Rogers, A. Terry, L. Clifton","doi":"10.3233/BSI-170167","DOIUrl":"https://doi.org/10.3233/BSI-170167","url":null,"abstract":"Protein small angle scattering (SAS) has become increasing important in structural biochemistry, due to the increased performance and specification of new instruments and advances in the software and hardware used to analyse the data. Whilst all of this is encouraging, there is a lack of standardised experimental methodology within the community. Although a number of protein standards are currently used in SAS experiments to allow accurate molecular weight determination, each has specific advantages and disadvantages. We therefore propose the use of a mutated monomeric enhanced green fluorescent protein, as a protein standard, abbreviated to m-eGFP. It has a number of advantages over the currently used protein standards, for example it is cheap and easy to produce. It can be expressed in large amounts (>40 mg/L) in both hydrogenated and deuterated form. The mutation means it is highly monodisperse and GFP being a beta-barrel structure is thermodynamically stable over a number of days, giving highly reproducible results. We therefore believe m-eGFP is a good protein standard for small angle scattering (SAS).","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-170167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43720760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

We must not forget that 99% of the total number of molecules present in a living organism is water 我们不能忘记，存在于生物体中的分子总数的99%是水

Biomedical Spectroscopy and Imaging Pub Date : 2017-12-27 DOI: 10.3233/BSI-170172

P. Haris

引用次数: 0

Application of motion history image (MHI) on dynamic fluorescent imaging for monitoring cerebral ischemia induced by occlusion of middle cerebral artery (MCA) in mouse brain 运动历史图像(MHI)在动态荧光成像监测小鼠大脑中动脉闭塞性脑缺血中的应用

Biomedical Spectroscopy and Imaging Pub Date : 2017-12-27 DOI: 10.3233/BSI-170170

M. Z. Ansari, A. Mujeeb

引用次数: 3

The evolution of biomedical EPR (ESR) 生物医学EPR（ESR）的发展

Biomedical Spectroscopy and Imaging Pub Date : 2017-06-20 DOI: 10.3233/BSI-150128

L. Berliner

{"title":"The evolution of biomedical EPR (ESR)","authors":"L. Berliner","doi":"10.3233/BSI-150128","DOIUrl":"https://doi.org/10.3233/BSI-150128","url":null,"abstract":"Since the first EPR/ESR spectrum of a paramagnetic substance was published over 70 years ago, the technical improvements did not occur until after/during World War II with the advent of radar technology. The approaches to biomedical problems started somewhat later with the real burst of activity starting after the birth of the spin label technique about 50 years ago. The applications to proteins, then membranes and nucleic acids, and later applications to cells and eventually in-vivo on small animals and now humans has led EPR/ESR to finally being recognized as a uniquely powerful technique in the toolbox of techniques probing macromolecules and their interactions, free radical biology and its eventual value as a diagnostic technique. This article gives an overview of EPR/ESR studies of biomedically related systems, including proteins and enzymes. It presents a very personal historical perspective, briefly reviews the origins of the technique and reflects on possible future directions. As with NMR, advances in molecular biology and technology drastically changed the nature and focus of the technique, particularly the site directed spin labeling method that has been invaluable in determining protein and macromolecular structure by both EPR and NMR.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-150128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45350596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Lawrence Berliner: Pioneer, educator and champion of biomedical EPR spectroscopy Lawrence Berliner:生物医学EPR光谱学的先驱、教育家和冠军

Biomedical Spectroscopy and Imaging Pub Date : 2017-06-20 DOI: 10.3233/BSI-150131

P. Haris

引用次数: 0

Stroke Onset Time Determination Using MRI Relaxation Times without Non-Ischaemic Reference in A Rat Stroke Model. 大鼠脑卒中模型中无非缺血性参考的MRI松弛时间测定脑卒中发作时间。

Biomedical Spectroscopy and Imaging Pub Date : 2017-06-20 DOI: 10.3233/BSI-160155

Terence J T Norton, Marcelo Pereyra, Michael J Knight, Bryony M McGarry, Kimmo T Jokivarsi, Olli H J Gröhn, Risto A Kauppinen

{"title":"Stroke Onset Time Determination Using MRI Relaxation Times without Non-Ischaemic Reference in A Rat Stroke Model.","authors":"Terence J T Norton, Marcelo Pereyra, Michael J Knight, Bryony M McGarry, Kimmo T Jokivarsi, Olli H J Gröhn, Risto A Kauppinen","doi":"10.3233/BSI-160155","DOIUrl":"https://doi.org/10.3233/BSI-160155","url":null,"abstract":"Background: Objective timing of stroke in emergency departments is expected to improve patient stratification. Magnetic resonance imaging (MRI) relaxations times, T2 and T1ρ , in abnormal diffusion delineated ischaemic tissue were used as proxies of stroke time in a rat model.Methods: Both 'non-ischaemic reference'-dependent and -independent estimators were generated. Apparent diffusion coefficient (ADC), T2 and T1ρ , were sequentially quantified for up to 6 hours of stroke in rats (n = 8) at 4.7T. The ischaemic lesion was identified as a contiguous collection of voxels with low ADC. T2 and T1ρ in the ischaemic lesion and in the contralateral non-ischaemic brain tissue were determined. Differences in mean MRI relaxation times between ischaemic and non-ischaemic volumes were used to create reference-dependent estimator. For the reference-independent procedure, only the parameters associated with log-logistic fits to the T2 and T1ρ distributions within the ADC-delineated lesions were used for the onset time estimation.Result: The reference-independent estimators from T2 and T1ρ data provided stroke onset time with precisions of ±32 and ±27 minutes, respectively. The reference-dependent estimators yielded respective precisions of ±47 and ±54 minutes.Conclusions: A 'non-ischaemic anatomical reference'-independent estimator for stroke onset time from relaxometric MRI data is shown to yield greater timing precision than previously obtained through reference-dependent procedures.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-160155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35148559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Magnetic resonance microimaging of cancer cell spheroid constructs 癌症细胞球形结构的磁共振显微成像

Biomedical Spectroscopy and Imaging Pub Date : 2017-06-20 DOI: 10.3233/BSI-150130

K. Momot, Onur Bas, N. P. Holzapfel, D. Loessner

{"title":"Magnetic resonance microimaging of cancer cell spheroid constructs","authors":"K. Momot, Onur Bas, N. P. Holzapfel, D. Loessner","doi":"10.3233/BSI-150130","DOIUrl":"https://doi.org/10.3233/BSI-150130","url":null,"abstract":"BACKGROUND \u0000 \u0000Hydrogel-based cell cultures are excellent tools for studying physiological events occurring in the growth and proliferation of cells, including cancer cells. Diffusion magnetic resonance is a physical technique that has been widely used for the characterisation of biological systems as well as hydrogels. In this work, we applied diffusion magnetic resonance imaging (MRI) to hydrogel-based cultures of human ovarian cancer cells. \u0000 \u0000METHODS \u0000 \u0000Diffusion-weighted spin-echo MRI measurements were used to obtain spatially-resolved maps of apparent diffusivities for hydrogel samples with different compositions, cell loads and drug (Taxol) treatment regimes. The samples were then characterised using their diffusivity histograms, mean diffusivities and the respective standard deviations, and pairwise Mann-Whitney tests. The elastic moduli of the samples were determined using mechanical compression testing. \u0000 \u0000RESULTS \u0000 \u0000The mean apparent diffusivity of the hydrogels was sensitive to the polymer content, cell load and Taxol treatment. For a given sample composition, the mean apparent diffusivity and the elastic modulus of the hydrogels exhibited a negative correlation. \u0000 \u0000CONCLUSIONS \u0000 \u0000Diffusivity of hydrogel-based cancer cell culture constructs is sensitive to both cell proliferation and Taxol treatment. This suggests that diffusion-weighted imaging is a promising technique for non-invasive monitoring of cancer cell proliferation in hydrogel-based, cellularly-sparse 3D cell cultures. The negative correlation between mean apparent diffusivity and elastic modulus suggests that the diffusion coefficient is indicative of the average density of the physical microenvironment within the hydrogel construct.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-150130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49468665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A small sided game session affects salivary metabolite levels in young soccer players 小型比赛会影响年轻足球运动员唾液代谢物水平

Biomedical Spectroscopy and Imaging Pub Date : 2017-06-20 DOI: 10.3233/BSI-150132

D. Cicero, S. D. Marino, V. Dinallo, M. Pieri, Vincenzo Summa, A. Desideri, A. Bernardini, F. Perondi, S. D'Ottavio

{"title":"A small sided game session affects salivary metabolite levels in young soccer players","authors":"D. Cicero, S. D. Marino, V. Dinallo, M. Pieri, Vincenzo Summa, A. Desideri, A. Bernardini, F. Perondi, S. D'Ottavio","doi":"10.3233/BSI-150132","DOIUrl":"https://doi.org/10.3233/BSI-150132","url":null,"abstract":"Abstract. \u0000BACKGROUND: The use of saliva for monitoring metabolic variations in physical exercise and in different sports gained \u0000ground in recent years. Several studies showed that saliva reflects biochemical changes useful for analytical purposes in clinical \u0000investigations and in physiological research. \u0000OBJECTIVE: The aim of this study was to explore the profile of salivary metabolite changes due to a session of small sided \u0000games (SSG) in elite soccer players, searching for a correlation between metabolic changes and athlete performance as GPSmeasured \u0000distances covered in the match. \u0000METHODS: Ten under-20 elite soccer players participated to the study. The game had an overall duration of 24 min and it \u0000consisted of 4 bouts of 6 min duration with 2 min passive recovery between exercise bouts. Saliva samples were collected \u0000before and after the game and physiological parameters evaluated, namely the distances covered by players and blood lactate. \u0000Samples were analyzed by Nuclear Magnetic Resonance spectroscopy. Orthogonal Projection of Latent-Structure (OPLS) was \u0000used to process the data. \u0000RESULTS: Multivariate data analysis showed that the SSG session affected salivary metabolite levels in players. We observed \u0000no relationship between concentrations of hematic and salivary lactate, nor found any changes in the metabolic profiles that \u0000correlate with the blood lactate values. Among the identified metabolites, taurine was instead found to correlate with distances \u0000covered by players during the game. \u0000CONCLUSIONS: Altogether these results point to a potential use of saliva to follow metabolic changes during an athletic \u0000competition, and opens the possibility of using this non-invasive biofluid for the study of athlete training state and performance.","PeriodicalId":44239,"journal":{"name":"Biomedical Spectroscopy and Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BSI-150132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48548339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7