Cluj Veterinary Journal最新文献

The use of Complementary medicine and Pneumo-Acupuncture to treat muscle atrophy and chronic respiratory disorders in a dog: A case report 使用补充医学和气针治疗狗的肌肉萎缩和慢性呼吸系统疾病:一个病例报告

Cluj Veterinary Journal Pub Date : 2022-06-28 DOI: 10.52331/cvj.v27i1.38

M. Dragomir, A. Ardelean, Lorena Lloret Nadal, C. Ober

引用次数: 0

ETIOPATHOGENETIC MECHANISM IN DOG SYRINGOMYELIA 犬脊髓空洞的发病机制

Cluj Veterinary Journal Pub Date : 2022-06-28 DOI: 10.52331/cvj.v27i1.33

Caroline-Maria Lacatus

引用次数: 0

Splenic hematoma and pelvic bladder in a spayed German shepherd mongrel bitch (Canis lupus familiaris). A case report. 一只已绝育的德国杂种母狗(狼犬)脾脏血肿和盆腔膀胱。一份病例报告。

Cluj Veterinary Journal Pub Date : 2022-06-28 DOI: 10.52331/cvj.v27i1.36

I. Vasiu, Oros Valentin Nicuşor, Aştilean Andreea Niculina, Melega Iulia, Rusu Andreea, Purdoiu Robert, T. Flaviu, Vasiu Mariana, Mocanu Emanuel Mihai, Ober Ciprian Andrei

{"title":"Splenic hematoma and pelvic bladder in a spayed German shepherd mongrel bitch (Canis lupus familiaris). A case report.","authors":"I. Vasiu, Oros Valentin Nicuşor, Aştilean Andreea Niculina, Melega Iulia, Rusu Andreea, Purdoiu Robert, T. Flaviu, Vasiu Mariana, Mocanu Emanuel Mihai, Ober Ciprian Andrei","doi":"10.52331/cvj.v27i1.36","DOIUrl":"https://doi.org/10.52331/cvj.v27i1.36","url":null,"abstract":": Splenic hematomas represent the most encountered splenic benign masses in dogs (Canis lupus familiaris), and they are usually secondary to splenic nodular hyperplasia. In most spayed bitches with urinary incontinence (UI), pelvic bladders are a common finding. In addition, ovariohysterectomy (OHE), hormonal imbalances, and various anatomical anomalies are responsible for the onset of urethral sphincter mechanism incompetence (USMI). This case report highlights the aggravating aspect caused by a splenic hematoma to develop a pelvic bladder in a mongrel bitch, that was sterilized seven years ago. A 14-years-old spayed German shepherd was presented to the Emergency Veterinary Hospital in Cluj-Napoca, with a history of apathy, incontinence, and foul kennel smell, for several months. The diagnostic was based on anamnesis, medical history, imagistic, and routine laboratory assays. The main findings were the presence of a pelvic bladder, splenic hematoma, and chronic cholecystitis. The bitch was admitted for 14 days. Surgical intervention was required, so a splenectomy was performed. Besides the surgical management and the supportive care, the bitch also received treatment for UI with phenylpropanolamine (PPA; Propalin 5%; 1.2 mg/kg 12h PO prn). Three days after surgery and treatment, the bitch recovered the urinary tonus, and UI was absent. The bitch was discharged two weeks after the surgical intervention. The presence of splenic hematomas can precipitate the development of UI by partially translocating the urinary bladder into the pelvic cavity (i.e., pelvic bladder), especially in old spayed bitches.","PeriodicalId":430924,"journal":{"name":"Cluj Veterinary Journal","volume":"192 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128346378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing two strategies for production of murine ascites with anti-SARS-CoV-2 monoclonal antibodies 评估用抗sars - cov -2单克隆抗体生产小鼠腹水的两种策略

Cluj Veterinary Journal Pub Date : 2021-12-31 DOI: 10.52331/cvj.v26i3.31

Joel Javier Pérez-Paz, R. Blanco, D. Dorta, A. Domínguez, Maylin Pérez-Bernal, Celia Tamayo, Carlos Hernández, Ricardo Piña, Javier Diaz, Shaylí Pérez, I. Pasarón, Enrique Pérez

{"title":"Assessing two strategies for production of murine ascites with anti-SARS-CoV-2 monoclonal antibodies","authors":"Joel Javier Pérez-Paz, R. Blanco, D. Dorta, A. Domínguez, Maylin Pérez-Bernal, Celia Tamayo, Carlos Hernández, Ricardo Piña, Javier Diaz, Shaylí Pérez, I. Pasarón, Enrique Pérez","doi":"10.52331/cvj.v26i3.31","DOIUrl":"https://doi.org/10.52331/cvj.v26i3.31","url":null,"abstract":"Studies were conducted to improve the production of murine ascites with monoclonal antibodies that recognize SARS-CoV-2 proteins. BALB/c mice were primed with 0.5 mL of mineral oil into the abdominal cavity. Seven days after priming, mice were divided in two groups: the group 1 was inoculated intraperitoneally with 2x106 cells/mL of MAb-secreting hybridomas against the nucleocapsid and spike proteins of SARS-CoV-2; the group 2 was injected simultaneously with the same inoculum of hybridoma cells and mineral oil, 18 days after priming. No disturbances or suffering signals were observed in mice from both groups, suggesting that double administration of mineral oil did not produce significant distress with respect to the single dose used for priming, and that none of the hybridoma cell lines were particularly aggressive for the inoculated mice. Ascites was collected in 90.48% and 97.68% of mice from groups 1 and 2, respectively. Ascites was not collected in 7.42% of all mice. The main cause was they never developed ascites tumors but no solid tumors were observed either. The volume of ascitic fluid per mouse was increased significantly in mice from group 2, and there were no significant differences between groups in terms of the concentration of IgG in clarified ascites. According to these results, to obtain higher amounts of MAb the strategy applied in group 2 should be used, since it showed the best results in the development of ascites tumors and it significantly increased the volume of ascites fluid per mouse. This could allow the use of fewer animals for ascites production, which is an ethical and economic benefit.","PeriodicalId":430924,"journal":{"name":"Cluj Veterinary Journal","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114865618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The link between mammary cancer, excessive adipose tissue and cholesterol 乳腺癌，过多的脂肪组织和胆固醇之间的联系

Cluj Veterinary Journal Pub Date : 2021-12-31 DOI: 10.52331/cvj.v26i3.35

C. Munteanu, B. Pop

引用次数: 0

Glaucoma in Dogs 狗的青光眼

Cluj Veterinary Journal Pub Date : 2021-12-31 DOI: 10.52331/cvj.v26i3.34

A. Zăvoi, A. Enache

引用次数: 0

Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows 将屠宰的老牛的卵母细胞注入胞浆内精子生产可存活的牛胚胎

Cluj Veterinary Journal Pub Date : 2021-04-21 DOI: 10.52331/cvj.v26i1.8

M. Cenariu, M. Borzan, S. Dan, Remus Chiorean, E. Páll

{"title":"Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows","authors":"M. Cenariu, M. Borzan, S. Dan, Remus Chiorean, E. Páll","doi":"10.52331/cvj.v26i1.8","DOIUrl":"https://doi.org/10.52331/cvj.v26i1.8","url":null,"abstract":"Abstract: (1) Background: Intracytoplasmic sperm injection (ICSI) is currently used to increase fertilization success by avoiding several oocyte or sperm deficiencies that would normally prevent conception after in vivo fertilization or classical in vitro fertilization. This paper aimed at improving the in vitro fertilization protocol of bovine oocytes, harvested from old cows after slaughtering, using intracytoplasmic sperm injection; (2) Methods: Oocytes were harvested by puncture of follicles from ovaries obtained from slaughtered old cows, followed by aspiration. Out of the 127 cumulus-oocyte complexes that were harvested, 84 (66.14%) were declared suitable for cultivation, after morphological evaluation. Following oocyte maturation for 22 hours, 77 cumulus-oocyte complexes were morphologically intact and could undergo the steps required for intracytoplasmic injection of spermatozoa. Frozen-thawed bull semen was used for ICSI and the 77 fertilized oocytes were kept for 24 hours in an atmosphere enriched with 5% CO2.; (3) Results: Fertilized oocytes transformed into 46 zygotes (fertilization rate of 59.74%), while after 168 h of cultivation 38 transferable compact morulae or early blastocysts were obtained; (4) Conclusions: Intracytoplasmic sperm injection can represent a viable alternative to classical IVF, when oocytes or sperm with lower fertility are used.","PeriodicalId":430924,"journal":{"name":"Cluj Veterinary Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128803140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IN UTERO TRANSPLANTATION OF HUMAN STEM CELLS USING AN ANIMAL MODEL: A COMPARISON BETWEEN TWO TECHNIQUES 使用动物模型的人干细胞子宫移植:两种技术之间的比较

Cluj Veterinary Journal Pub Date : 2009-03-16 DOI: 10.52331/cvj.v15i1.2

D. Groza, E. Pall, M. Cenariu, P. Pop, N. Costin, I. Groza

{"title":"IN UTERO TRANSPLANTATION OF HUMAN STEM CELLS USING AN ANIMAL MODEL: A COMPARISON BETWEEN TWO TECHNIQUES","authors":"D. Groza, E. Pall, M. Cenariu, P. Pop, N. Costin, I. Groza","doi":"10.52331/cvj.v15i1.2","DOIUrl":"https://doi.org/10.52331/cvj.v15i1.2","url":null,"abstract":"\u0000 \u0000 \u0000Objective: The purpose of this study was to assess the feasibility of in utero stem cell transplantation of human umbilical cord blood stem cells in fetal sheep and to compare two different techniques of in utero transplantation, namely ultrasound-guided in utero transplantation and in utero transplantation after midline celiotomy. \u0000Study design: Umbilical cord blood units were collected from term deliveries, after obtaining written informed consent. Human cord blood–derived, CD34+ stem cells were injected into the peritoneal cavity of 60- to 65-day-old ovine fetuses by using 2 different techniques: ultrasound-guided transabdominal percutaneous needle puncture and midline celiotomy with the exposure of the pregnant uterus. Engraftment was determined after birth by flow cytometry with use of human-specific anti-CD 34/45 antibodies. \u0000Results: We obtained a total of 3 chimeric lambs. Using the midline celiotomy technique the fetal loss rate was 75% and only 33,3% when using ultrasound-guided transabdominal percutaneous needle puncture technique. Engraftment of donor cells was found in all fetuses, with a mean level of 1.4% in fetal peripheral blood and 3.3% in fetal bone marrow. \u0000Conclusion: This preliminary study indicates that in utero stem cell transplantation of human hematopoietic cord blood stem cells in fetal lambs is feasible and effective in terms of hematopoietic engraftment. We also concluded that the ultrasound-guided transabdominal percutaneous needle puncture technique is more effective than performing a midline celiotomy in terms of fetal loss rate. \u0000 \u0000 \u0000","PeriodicalId":430924,"journal":{"name":"Cluj Veterinary Journal","volume":"1075 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116023484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RAT BONE MARROW MESENCHYMAL STEM CELLS ISOLATION, CULTIVATION AND DIFFERENTIATION 大鼠骨髓间充质干细胞的分离、培养与分化

Cluj Veterinary Journal Pub Date : 2009-03-16 DOI: 10.52331/cvj.v15i1.6

E. Páll, I. Groza, O. Sorițău, C. Tomuleasa, M. Cenariu, D. Groza, Teodora Vlasiu

引用次数: 1

DETERMINATION OF PATHOGEN RESISTANCE TO STREPTOMYCIN 病原菌对链霉素耐药性的测定

Cluj Veterinary Journal Pub Date : 2009-03-16 DOI: 10.52331/cvj.v15i1.13

A. Chereji, R. Chereji

引用次数: 0