Proteoforms - Concept and Applications in Medical Sciences最新文献

Prolactin Proteoform Pattern Changed in Human Pituitary Adenoma Relative to Control Pituitary Tissues 人垂体腺瘤与对照垂体组织中泌乳素变形模式的改变

Proteoforms - Concept and Applications in Medical Sciences Pub Date : 2020-06-15 DOI: 10.5772/intechopen.92836

X. Zhan, Shehua Qian

{"title":"Prolactin Proteoform Pattern Changed in Human Pituitary Adenoma Relative to Control Pituitary Tissues","authors":"X. Zhan, Shehua Qian","doi":"10.5772/intechopen.92836","DOIUrl":"https://doi.org/10.5772/intechopen.92836","url":null,"abstract":"PRL gene-encoded prolactin is synthesized in the ribosome in the pituitary and then secretes into blood circulation to reach its target organ and exerts its biological roles, for example, involving in production, growth, development, immunoregulation, and metabolism. Multiple post-translational modifications and other unknown factors might be involved in this process to cause different prolactin proteoforms with differential isoelectric point (p I ) and relative mass ( M r ). Pituitary adenomas are the common disease occurring in pituitary organ to affect the endocrine system. Two-dimensional gel electrophoresis (2DGE) was used to separate prolactin proteoforms according to their p I and M r , followed by identification with Western blot and mass spectrometry (MS) analyses. Six prolactin proteoforms were identified in control pituitary tissues, and this prolactin proteoform pattern was significantly changed in different hormone subtypes of nonfunctional pituitary adenomas (NF − , LH + , FSH + , and LH + / FSH + ) and prolactinomas (PRL + ). Further, bioinformatics analysis revealed that different prolactin proteoforms might bind to different short- or long-PRL receptor-mediated signaling pathways. These findings clearly demonstrated that prolactin proteoform pattern existed in human pituitary and changed in different subtypes of pituitary adenomas. It is the scientific data to in-depth study prolactin functions, and to discover the prolactin proteoform biomarkers for PRL-related adenomas. controls, with 2DGE and MS. These findings provide the scientific data to in-depth study the PRL functions and to discover PRL proteoform biomarker for PRL-related adenomas.","PeriodicalId":429683,"journal":{"name":"Proteoforms - Concept and Applications in Medical Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132541401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Proteoforms: General Concepts and Methodological Process for Identification 异形:一般概念和鉴定的方法过程

Proteoforms - Concept and Applications in Medical Sciences Pub Date : 2019-12-23 DOI: 10.5772/intechopen.89914

J. S. Araújo, O. Machado

引用次数: 1

Proteoforms in Acute Leukemia: Evaluation of Age- and Disease-Specific Proteoform Patterns 急性白血病的蛋白质形成:年龄和疾病特异性蛋白质形成模式的评估

Proteoforms - Concept and Applications in Medical Sciences Pub Date : 2019-12-19 DOI: 10.5772/intechopen.90329

F. Hoff, A. Dijk, S. Kornblau

引用次数: 1

Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry 自上而下质谱法制备治疗蛋白的蛋白质形态

Proteoforms - Concept and Applications in Medical Sciences Pub Date : 2019-10-28 DOI: 10.5772/intechopen.89644

S. Hidayah, Manasia Gaikwad, Laura Heikaus, H. Schlüter

{"title":"Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry","authors":"S. Hidayah, Manasia Gaikwad, Laura Heikaus, H. Schlüter","doi":"10.5772/intechopen.89644","DOIUrl":"https://doi.org/10.5772/intechopen.89644","url":null,"abstract":"A characteristic of many proteoforms, derived from a single gene, is their similarity regarding the composition of atoms, making their analysis very challenging. Many overexpressed recombinant proteins are strongly associated with this problem, especially recombinant therapeutic glycoproteins from large-scale productions. In contrast to small molecule drugs, which consist of a single defined molecule, therapeutic protein preparations are heterogenous mixtures of dozens or even hundreds of very similar species. With mass spectrometry, currently high-quality spectra of intact proteoforms can be obtained only, if the complexity of the mixture of individual proteoform-ions, entering the gas phase at the same time is low. Thus, prior to mass spectrometric analysis, an effective separation is required for getting fractions with a low number of individual proteoforms. This is especially true not only for recombinant therapeutic proteins, because of their huge heterogeneity, but also relevant for top-down proteomics. Purification of proteoforms is the bottleneck in analyzing intact proteoforms with mass spectrometry. This review is focusing on the current state of the art, especially of liquid chromatography for preparing proteoforms for mass spectrometric top-down analysis. The topic of therapeutic proteins has been chosen, because this group of proteins is most challenging regarding their proteoform analysis.","PeriodicalId":429683,"journal":{"name":"Proteoforms - Concept and Applications in Medical Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126340024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0