Proteoforms: General Concepts and Methodological Process for Identification

J. S. Araújo, O. Machado
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引用次数: 1

Abstract

The term proteoform is used to denote all the molecular forms in which the protein product of a single gene can be found. The most frequent processes that lead to transcript modification and the biological implications of these changes observed in the final protein product will be discussed. Proteoforms arising from genetic variations, alternatively spliced RNA transcripts and post-translational modifications will be commented. This chapter will present an evolution of the techniques used to identify the proteoforms and the importance of this identification for understanding of biological processes. This chapter highlights the fundamental concepts in the field of top-down mass spectrometry (TDMS), and provides numerous examples for the use of knowledge obtained from the identification of proteoforms. The identification of mutant proteins is one of the emerging areas of proteogenomics and has the potential to recognize novel disease biomarkers and may point to useful targets for identification of therapeutic approaches.
异形:一般概念和鉴定的方法过程
proteoform这个术语是用来表示单个基因的蛋白质产物可以被发现的所有分子形式。我们将讨论导致转录物修饰的最常见过程以及在最终蛋白产物中观察到的这些变化的生物学意义。由遗传变异、选择性剪接RNA转录物和翻译后修饰引起的蛋白质形态将被评论。本章将介绍用于识别变形形态的技术的演变,以及这种识别对理解生物过程的重要性。本章重点介绍了自顶向下质谱法(TDMS)领域的基本概念,并提供了许多使用从鉴定变形形态中获得的知识的例子。突变蛋白的鉴定是蛋白质基因组学的新兴领域之一,具有识别新的疾病生物标志物的潜力,并可能为鉴定治疗方法指明有用的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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