Egyptian Journal of Haematology最新文献

{"title":"Pulmonary hypertension as a risk factor in JAK2-positive polycythemia rubra vera","authors":"S. Ahmed, A. Rashad, A. Nafady, A. Shazly, Areej Alkhateeb, Mohamed Elsenbesya","doi":"10.4103/ejh.ejh_9_21","DOIUrl":"https://doi.org/10.4103/ejh.ejh_9_21","url":null,"abstract":"Introduction The occurrence of primary pulmonary arterial hypertension (PAH (in primary myeloid proliferation neoplasms seems quite high, up to 22% in polycythemia vera. Polycythemia rubra vera (PRV) is one of the myeloproliferative neoplasms. We aimed to study the incidence of PAH among patients with PRV. Patients and methods A prospective cross-section study was performed on 60 (PRV) patients with PRV confirmed by bone marrow and JAK2 positivity. Abdominal ultrasonography, transthoracic echocardiography, and computed tomography chest were done to estimate the pulmonary hypertension (HTN) and exclude other chest diseases. Results Among the studied 60 patients, we found 14 patients with pulmonary HTN (23.4%) who had significantly increased incidence of comorbidities than patients with normal pulmonary pressure (P=0.009). Moreover, there were statistically significant differences in the size of spleen by ultrasound between the two groups (P=0.008). Patients with pulmonary HTN had a higher hemoglobin level compared with those with normal pulmonary pressure (P=0.006). There was a significant positive correlation between pulmonary pressure and existing comorbidities (diabetes mellitus, HTN, or both) but no correlation with the size of the spleen, hemoglobin level, or white blood cells. Conclusion The prevalence of PAH in JAK2-positive patients with PRV is 76.6%, and there was significant relationship between hematological parameters (hemoglobin, white blood cells, lactate dehydrogenase, and urea level and PAH in JAK2-positive patients with PRV.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"70 - 74"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44475469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study between different alloadsorption techniques in warm autoimmune hemolytic anemia","authors":"N. Emam, Soha Ezzelarab, N. Hassan, Heba El Saeyed, N. Nabih, G. Hamed","doi":"10.4103/ejh.ejh_52_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_52_20","url":null,"abstract":"Background Autoantibodies in patients with autoimmune hemolytic anemia (AIHA) are encountered with difficulties in ABO grouping and cross-matching; moreover, they may mask the presence of alloantibodies, leading to hemolytic transfusion reaction. Therefore, an efficient and time-saving method is required to detect alloantibodies underlying autoantibodies for safe transfusion in AIHA. Aim To compare the efficiency of different alloadsorption techniques in detection of alloantibodies after complete removal of autoantibodies. Patients and methods A total of 70 patients with warm AIHA were enrolled in this study; antibody screening was done by using screening cells (I+II+III cells). Allogenic adsorption was performed using conventional, polyethylene glycol (PEG), and low ionic strength solution (LISS)/papain methods followed by rescreening to ensure the adsorption of the autoantibodies, and whenever screening test revealed the presence of alloantibodies, antibody identification was done. Antibody identification and cross-matching with phenotyped red cells were done by the plasma obtained with this method. The best adsorption method was chosen depending on its ability to preserve the alloantibody. Results A significant difference was found among the three alloadsorption methods regarding number of alloadsorptions and time for complete autoantibodies removal. PEG showed the lowest mean number of alloadsorptions (2.6±1.2) followed by LISS/papain (3.1±1.5) compared with the conventional method (3.7±1.4) (P<0.001). Regarding the time, PEG was the most rapid method (38.6±17.6 min) followed by LISS/papain (45.9±22.8 min) and then the conventional method (110.1±14.4 min) (P<0.001). However, no significant difference was found between PEG and LISS/papain (P=0.014). Alloantibodies were detected in 35% of cases, with predominance of anti-Rh system (61%), mainly anti-c and anti-E. Alloantibodies belonging to Rh and Kidd were best identified in adsorbed plasma by LISS/papain in comparison with PEG and the conventional techniques. Conclusion Among the evaluated methods, LISS/papain displayed the highest diagnostic accuracy, sensitivity, and specificity of alloantibody detection. Compared with the conventional method, LISS/papain and PEG minimized the time and number of alloadsorptions, enhancing turnaround time and reducing the labor of pretransfusion testing in AIHA.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"105 - 110"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43047248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Charlson comorbidity index on vascular events and survival in Philadelphia chromosome-negative myeloproliferative neoplasms","authors":"Shima A. Ahmed, M. Aly","doi":"10.4103/ejh.ejh_62_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_62_20","url":null,"abstract":"Background Thromboembolic events and bleeding episodes are the main complications of myeloproliferative neoplasms (MPNs). Comorbidity is a well-known independent prognostic factor for patients with cancer that affects overall survival. Aim Our aim is to detect comorbidities among patients with Philadelphia chromosome-negative MPNs and to study how comorbidities affect survival and vascular events. Patients and methods A total of 190 patients with Philadelphia chromosome-negative MPNs were diagnosed between January 2014 and December 2018 in South Valley and Assiut University Hospitals. Charlson Comorbidity Index (CCI) was applied to evaluate patients. Median age was 57.5 years (range, 20–85). Overall, 50 (26.3%) patients had no comorbidities (low), 83 (43.7%) had a CCI 1–2 (moderate), and 57 (30%) had a CCI more than 2 (severe). Results There were no significant differences between patients with CCI less than 2 and patients with CCI more than or equal to 2 regarding sex, splenomegaly, white blood cell count, platelet count, and JAK II positivity. Significantly older ages (64.7±9 vs. 50.8±9, P<0.001), lower hemoglobin (5±12.3 vs. 14.6±5, P=0.046), and higher lactate dehydrogenase (P=0.004) were detected in patients with CCI more than or equal to 2. A significant association regarding pruritus and erythromelalgia was found among patients who had CCI more than or equal to 2 compared with patients with CCI less than 2 (P=0.038 and 0.025, respectively). Thrombosis was more frequent with CCI more than or equal to 2 (P<0.001) as well as bleeding (P=0.042). Overall survival and progression-free survival differed significantly between the different CCI groups (P≤0.001 and 0.003, respectively). Conclusion In conclusion, comorbidity has a negative prognostic effect on patients with Philadelphia chromosome-negative MPNs, which might elicit to be incorporated into prognostic models, with larger prospective studies needed for validation.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"111 - 115"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41394991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia","authors":"A. Gawaly, Rasha A. Elkholy, R. Hagag, A. A. Abd El-Lateef, Alzahraa A. Allam","doi":"10.4103/ejh.ejh_61_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_61_20","url":null,"abstract":"Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. Neuron-glial antigen 2 (NG2) is not expressed by normal hematopoietic stem cells but expressed on blast cells in adult AML. NG2 has been incorporated in diagnostic panels for immunophenotyping of leukemic patients because of its positive predictive value for Mixed Lineage Leukemia (MLL) rearrangements. Aims To assess NG2 expression in adult patients with AML and its correlation with disease-free survival. Patients and methods A total of 60 patients were divided into two groups: 40 newly diagnosed patients with AML and 20 patients diagnosed as having hypersplenism used as a control group. Leukemic patients were diagnosed on the basis of clinical presentation, morphological and cytochemical examination of peripheral blood and bone marrow smears, as well as immunophenotyping criteria for diagnosis of AML. NG2 expression was evaluated in the two groups using flow cytometry. Results No significant differences were found in both age and sex in different patient groups. NG2 expression in the patient group versus control group showed a statistically significant difference. There was no statistically significant difference regarding complete blood count, lactate dehydrogenase, and erythrocyte sedimentation rate, as well as blast percentage in the peripheral blood and in the bone marrow on comparing NG2-positive group and NG2-negative group. There is a significant increase in disease-free survival and overall survival in the negative NG2 expression than in the positive NG2 expression group (P=0.043). Conclusion NG2 expression has a major role in the outcome of patients with AML. NG2 expression analysis can be used as a prognostic marker in newly diagnosed patients with AML. NG2 could be a target for therapy by using anti-NG2 antibody in a subset of patients with AML who do not respond to conventional therapy.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"75 - 82"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47493296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum hepcidin and its relationship to iron homeostasis in obese anemic patients","authors":"Shaza Alkourashy, Walaa El-Salakawy, Alia M. Saeed","doi":"10.4103/ejh.ejh_44_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_44_20","url":null,"abstract":"Context Obesity is an increasingly recognized medical problem in both high-income and low-income countries owing to poor dietary habits and physical inactivity. This is frequently associated with dysregulated iron homeostasis. Iron deficiency in obese patients might be attributable to imbalanced diet or increased bodily demands. However, a role of enhanced hepcidin expression as a result of proinflammatory milieu in this setting has been contemplated in the recent years. Objectives First, we aimed at the measurement of serum hepcidin levels in obese anemic individuals. Second, we aimed at the assessment of the presence or absence of a relationship between its levels and deranged iron homeostatic profile. Patients and methods A total of 90 adult participants have been enrolled from the Clinical Hematology and Oncology Unit, Internal Medicine Department, Center ‘X,’ City ‘Z.’ Country ‘Y’. They have been divided into group I: 30 obese anemic patients, group II: 30 obese nonanemic patients, and group III: 30 healthy age-matched and sex-matched controls. Serum hepcidin levels were assayed using hepcidin ELISA kits. Results Group I enjoyed much higher serum hepcidin values as compared with either group II or III. Hepcidin level was associated with significantly lower mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, serum iron, and serum ferritin levels. However, it was remarkably associated with higher BMI, red cell distribution width, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein levels. Conclusion There is a low state of chronic inflammation in obese anemic individuals associated with higher serum hepcidin levels. This results into repressed iron absorption and release by the liver and phagocytic system, contributing largely to obesity-associated iron deficiency.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"92 - 98"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49502184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seropositive coronavirus disease 2019 detection among apparently healthy voluntary blood donors: a preliminary step to catch asymptomatic cases as a potential viral spread route","authors":"Sahar Abdelmaksoud, Mariam K. Youssef, Mahmoud Aboulmagd, M. Abdelmaksoud","doi":"10.4103/ejh.ejh_59_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_59_20","url":null,"abstract":"Background Despite the prevailing pandemic of coronavirus disease 2019, there are no recommendations available yet for serological screening of apparently healthy asymptomatic blood donors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. Objective To explore the seropositivity for SARS-CoV-2 antibodies in serum samples of a group of randomly selected Egyptian voluntary blood donors who met the eligibility criteria for blood donation as a preliminary step to catch apparently healthy asymptomatic participants who constitute an important source of viral spread in the community. Patients and methods The first phase of our study design plan included 100 anonymous serum samples of a group of voluntary blood donors who donated blood at Ain-Shams University Hospitals’ blood bank during the period from the second week of September to the end of October 2020. After completion of the steps of donation, donors’ stored serum samples were screened for SARS-CoV-2-specific immunoglobulin (Ig)M and IgG antibodies. The next phase of our study design plan is intended to include larger samples of known apparently healthy asymptomatic blood donors in an attempt to reach seropositive patients to confirm the results by PCR and validate the testing. Results A total of 100 anonymous serum samples were included in the study. Eleven (11%) samples were reactive for viral specific IgM antibodies, and 38 (38%) samples were reactive for viral specific IgG antibodies. Conclusion As the ongoing pandemic of coronavirus disease 2019 is continuing to demand urgent attention, screening of all asymptomatic apparently healthy voluntary blood donors for SARS-CoV-2 antibodies followed by viral RNA detection by PCR in seropositive donors could abort viral spread to others in blood centers and eventually to the rest of the community.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"65 - 69"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45197688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of soluble form of poliovirus receptor in newly diagnosed follicular lymphoma","authors":"N. Nabih, A. Kamal, M. Naguib","doi":"10.4103/ejh.ejh_32_21","DOIUrl":"https://doi.org/10.4103/ejh.ejh_32_21","url":null,"abstract":"Background Follicular lymphoma (FL) remains an incurable malignancy with heterogeneous clinical outcomes that necessitate a better understanding of disease biology. Poliovirus receptor (PVR/CD155) is markedly overexpressed in several human malignant tumors and it has a unique dual oncoimmunoregulatory role. However, the role of the soluble form of PVR (sCD155) in FL has not been fully elucidated. Methods Soluble PVR(sCD155) were measured in the sera of 50 patients newly diagnosed with FL by sandwich enzyme-linked immunosorbent assay and compared with those of 20 healthy control participants. Moreover, we evaluated its association with the clinicopathological parameters as well as response to chemotherapy in such patients. Results Pretreatment level of sCD155 was significantly higher in patients with FL than in control participants (P<0.001). Higher levels of sCD155 were associated with aggressive high-risk clinicopathological parameters, sCD155 levels were significantly higher in FL patients with B symptoms, advanced Ann Arbor stage III and IV, bulky disease, and high-risk cytogenetic (P-value=0.01, 0.048, 0.028 and <0.001, respectively). In addition, of the 50 patients, 24 (48%) achieved CR after 4–6 courses of chemotherapy (R-CHOP), while 26 (52%) were not in remission, and higher levels of sCD155 were associated with poor response to chemotherapy (P-value<0.001). Receiver operating characteristic curve was applied. Serum level of sCD155 higher than 4.8 ng/ml is a good predictor for poor response to chemotherapy (area under the curve: 0.857, sensitivity and specificity 88.46% and 75%, respectively). Conclusion PVR (CD155) is a potential therapeutic target that warrants further investigations and serum sCD155 may be used as a biomarker of treatment response and for predicting poor outcome in FL.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"116 - 122"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49455273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship of serum growth differentiating factor 15 with hepcidin in posttransplant adult Egyptian patients and its prognostic significance","authors":"M. Elrazzaz, M. Azzazi, Amal M Alafifi, H. Hegab, A. El-Ghammaz, M. Shazly","doi":"10.4103/ejh.ejh_12_21","DOIUrl":"https://doi.org/10.4103/ejh.ejh_12_21","url":null,"abstract":"Background Hepcidin is a small peptide that is produced in the liver that is most likely the major regulator of iron. Based upon the importance of iron, multiple mechanisms exist for the regulation of hepcidin. Iron levels, inflammation, erythropoiesis, and the combined effects of several proteins expressed on hepatocyte membranes are involved. Growth differentiation factor 15 (GDF15) is a member of the transforming growth factor-b. GDF15 expression level is usually low in resting cells but may be substantially increased following response to diverse cellular stress signals, such as hypoxia, inflammation, acute tissue injury, and during cancer progression. Aim The aim was to assess the relationship of serum GDF15 with hepcidin in posttransplant adult Egyptian patients as an assessment for iron overload and their relationship with posttransplantation complications. Patients and methods Serum GDF15 and hepcidin were measured using enzyme-linked immunosorbent assay in 45 postallogenic (23 patients) and autologous (22 patients) bone marrow transplanted patients 1 year after transplantation in comparison with 15 healthy controls recruited from the bone marrow transplantation unit, Ain Shams University Hospitals. Results Serum level of GDF15 and hepcidin level were elevated 1 year after allogenic and autologous transplantation patients in comparison with control group, with a statistically significant difference between patients and controls (P<0.001). GDF15 and hepcidin were positively correlated with ferritin level (P<0.001). GDF15 and ferritin were positively correlated with acute graft-versus-host disease (GVHD) and chronic GVHD (P=0.004 and 0.002, respectively), but hepcidin did not show any significant correlation with acute GVHD (P=0.110). Moreover, GDF15, hepcidin and ferritin were positively correlated with serum levels of alanine transferase and aspartate transferase in both autologous and allogenic transplanted patients. However, GDF15, hepcidin, and ferritin were not correlated with bacterial or viral infections in both allogenic and autologous groups of patients. Conclusion Both GDF15 and hepcidin are useful biomarkers for iron overload in late postallogenic and autologous bone marrow transplantation, and both can be used as a predictor for posttransplantation complications.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"123 - 132"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43087323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occult hepatitis C in peripheral blood mononuclear cells in thrombocytopenic patients after achieving sustained virological response with direct-acting antivirals","authors":"T. Elbedewy, Rasha A. Elkholy, E. Habba, S. A. Abd El-Khalik","doi":"10.4103/ejh.ejh_60_20","DOIUrl":"https://doi.org/10.4103/ejh.ejh_60_20","url":null,"abstract":"Background/aim Occult hepatitis C virus infection (OCI) may be present in resolved hepatitis C virus (HCV) after direct-acting antivirals (DAAs). DAAs may improve thrombocytopenia after achieving sustained virological response (SVR), but some patients may be manifested with thrombocytopenia after SVR. The aim of our study was to evaluate the presence of OCI in the peripheral blood mononuclear cells (PBMCs) in thrombocytopenic patients after achieving SVR with DAAs. Patients and methods This cross-sectional study included 32 thrombocytopenic patients who achieved SVR with DAAs and 32 HCV-infected patients who achieved SVR with DAAs without thrombocytopenia as a control group. All patients were investigated for HCV-ribonucleic acid (RNA) in PBMCs, hepatitis C virus core antigen (HCVcAg), platelet autoantibodies, and serum thrombopoietin. Results Among thrombocytopenic, non-thrombocytopenic, and both groups, HCV-RNA in PBMCs were detected in 40.63, 6.25, and 23.44%, respectively, although HCVcAg was detected in 31.25, 3.13, and 17.19%, respectively. The comparisons between thrombocytopenic and non-thrombocytopenic patients regarding HCV-RNA in PBMCs and HCVcAg were statistically significant. Comparisons between thrombocytopenic and non-thrombocytopenic and between positive and negative OCI patients regarding serum thrombopoietin were statistically insignificant. Platelet autoantibodies were detected in 56.25% of thrombocytopenic group. Conclusion Our study is the first to provide insights into the relationship between OCI and thrombocytopenia in patients with chronic HCV after achieving SVR with DAAs. The association between OCI and thrombocytopenia may be explained by autoimmune mechanism.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"83 - 91"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43652495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CXCR4 is a valuable poor prognostic marker for chronic lymphocytic leukemia: its higher level of expression is associated with inferior response to therapy and lower disease-free survival","authors":"I. Asfour, W. Elsalakawy, M. Sallam, M. Razzaz","doi":"10.4103/ejh.ejh_3_21","DOIUrl":"https://doi.org/10.4103/ejh.ejh_3_21","url":null,"abstract":"Background Chronic lymphocytic leukemia (CLL) is one of the common chronic lymphoproliferative disorders (lymphoid neoplasms). It is characterized by a progressive accumulation of functionally incompetent lymphocytes, which are usually monoclonal in origin. CLL is a heterogeneous disease; thus, in some cases, the disease progresses so slowly that treatment is not required, but in others, a more aggressive form of the disease develops. CXCR4 (CD184) is a chemokine and chemokine receptor pair playing critical roles in tumor genesis. It is overexpressed in many hematological malignancies including acute myeloid leukemia and non-Hodgkin’s lymphoma and generally correlates with a poor prognosis. Aim To evaluate the clinical utility of CXCR4 expression in patients with CLL as a possible predictor of disease outcome. Patients and methods This is a prospective study conducted on 33 adult patients with newly diagnosed CLL. Expression of CXCR4 was determined by flow cytometry on either peripheral blood or bone marrow samples. Correlation with the course of the disease and the known CLL prognostic parameters was done initially and after 6 months of follow-up. Results CXCR4 expression was positively correlated with absolute lymphocytic count, Rai score, β2 microglobulin, and lactate dehydrogenase levels. It was negatively correlated with hemoglobin and platelet counts, overall response rate, and 6-month disease-free survival. Conclusion We conclude that CXCR4 is a valuable poor prognostic marker for CLL. Its higher level of expression is associated with inferior response to therapy and lower disease-free survival.","PeriodicalId":42139,"journal":{"name":"Egyptian Journal of Haematology","volume":"46 1","pages":"99 - 104"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42465350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}