Pathologia最新文献

{"title":"Dependence of renal filtration capacity on the phenotype of chronic heart failure, indicators of systolic and diastolic heart function","authors":"V. Syvolap, V. Lysenko","doi":"10.14739/2310-1237.2021.1.223742","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.223742","url":null,"abstract":"The issue of changes in the filtration capacity of the kidneys depending on the structural-geometric and functional remodeling of the heart in different phenotypes of chronic heart failure, disorders of systolic and diastolic function of the left ventricle remains insufficiently studied.\u0000The aim of this work: to investigate the relationship between changes in the filtration capacity of the kidneys in patients with chronic heart failure of ischemic genesis depending on the phenotype, indicators of systolic and diastolic cardiac function.\u0000Materials and methods. After an informed consent was signed, 87 patients (men – n = 45, women – n = 42) with CHF of ischemic genesis with sinus rhythm, stage II A–B, II–IV functional class NYHA, who were divided into 2 groups, were involved in the study. Group 1 included patients with reduced (<45 %) left ventricular ejection fraction (HFrEF) (n = 57; 59.6 % men), group 2 – patients with CHF with preserved left ventricular ejection fraction (HFpEF) (n = 30; 36.6 % men). Patient groups were comparable in terms of age, sex, height, weight, and body surface area. Doppler echocardiographic examination was performed using the Esaote MyLab Eight (Italy). Glomerular filtration rate (GFR) was assessed using the CKD-EPI, MDRD, and Cockcroft-Gault formulas.\u0000Results. Renal dysfunction was registered in patients with CHF in 72 % of cases when calculated using the CKD-EPI formula, 66.7 % using the MDRD formula and 52.6 % using the Cockcroft-Gault formula. Only indicators of blood creatinine level (P = 0.011) and the calculated indicator of glomerular filtration rate according to Cockcroft-Gault (p = 0.047) depended on the types of CHF. GFR for all applied formulas (СKD-EPI, MDRD, Cockcroft-Gault) depended on age (r = -0.42; P = 0.001), height (r = 0.28; P = 0.08), weight (r = 0.31; P = 0.004), body surface area (r = 0.33; P = 0.002). A direct correlation between the creatinine level and the LV myocardial mass index, calculated using the Penn Convention formula, was established, however, the dependence of the creatinine level and GFR on the types of LV geometry was not revealed. The presence of an inverse correlation between LVEF and blood creatinine level (r = -0.3172; P = 0.003), between creatinine content and S lat (r = -0.531; Р = 0.006), a direct correlation between S lat and CKD-EPI (r = 0.5586; P = 0.004), MDRD (r = 0.6254; P = 0.001), Cockcroft-Gault (r = 0.4043; P = 0.045).\u0000Conclusions. In chronic heart failure of ischemic genesis with reduced left ventricular ejection fraction, a more pronounced impairment of the filtration capacity of the kidneys than in chronic heart failure patients with preserved left ventricular ejection fraction is observed. An inverse correlation was established between the LV EF and the blood creatinine level (r = -0.3172; P = 0.003). A decrease in the systolic velocity of movement of the lateral annulus fibrosus of the mitral valve is associated with a decrease in the filtration capacity of t","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"24 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82733028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergism of the pharmacological effect of glycine and thiotriazoline","authors":"I. F. Bielenichev, A. Yehorov","doi":"10.14739/2310-1237.2021.1.228919","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.228919","url":null,"abstract":"Aim. To establish the effect of the glycine and thiotriazoline (4:1) combination on the parameters of the energy production of the mitochondria of the rat brain under the conditions of simulating an acute cerebrovascular accident.\u0000Materials and methods. The experimental part was performed on 90 male Wistar rats weighing 180–200 g. To model acute cerebrovascular accident (ACVA) by ischemic type, a classical model was used, where common carotid arteries had been ligated bilaterally. All animals were divided into 5 experimental groups: the first – intact (sham-operated rats, which during anesthesia had their common carotid arteries separated without ligation); the second – rats with ACVA (control); the third – rats with ACVA, which underwent intragastric administration of glycine at a dose of 200 mg/kg in the form of a tablet mass every day for 4 days; the fourth – rats with stroke, which every day for 4 days underwent intragastric administration of a combination of glycine and thiotriazoline (4:1) in the form of a tablet mass; the fifth – rats with ACVA, which underwent every day intragastric administration of piracetam in the form of a tablet mass at a dose of 500 mg/kg.\u0000The sampling of biological material (brain) for research was carried out on the fourth day of the experiment according to the standard method. The mitochondrial fraction was isolated by differential centrifugation in a refrigerated centrifuge. The manifestations of mitochondrial dysfunction, have been spectrophotometrically studied according to the degree of opening of the mitochondrial pore (MP) and mitochondrial transmembrane potential (Ψ). The assessment of the intensity of oxidative stress was determined by the markers of protein oxidative modification – aldehydephenylhydrazone (APH) and ketonedinitrophenylhydrazone (KPH) – spectrophotometrically. The state of energy metabolism was determined by the level of the most significant intermediates – ATP, lactate, succinate and malate.\u0000Results. In the group of animals with ACVA modeling, we noted a decrease in the level of ATP in mitochondria by 1.55 times, an increase in lactate content by 1.1 times, a decrease in SDH activity by 3.8 times and a decrease in succinate concentration by 1.1 times relative to the corresponding data of intact groups. Administration of a combination of glycine and thiotriazoline to experimental animals on the fourth day of ACVA modeling led to a 1.9-fold decrease in the opening of the mitochondrial pore and an increase in the charge of the inner mitochondrial membrane by 1.2 times, an increase in ATP in the mitochondrial fraction by 1.1 times, an increase in SDH activity by 3 times, and the activity of NAD-MDH – by 3.7 times, against the background of a decrease in the level of APH by 76.6 % and KPH by 80.7 %, relative to the group of animals with modeling of stroke by ischemic type.\u0000Conclusions. Modeling of ACVA leads to the initiation of oxidative stress and the development of an imbalance of energy me","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"18 6","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72574103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthopedic treatment with fixed dentures taking into account the distribution and causes of defects in dental hard tissues","authors":"Yu. V. Klymiuk, Z. Ozhogan, L. Miziuk, V. Kryvanych, I. Yanishen","doi":"10.14739/2310-1237.2021.1.222967","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.222967","url":null,"abstract":"The aim of this work was to analyze the causes of complications of orthopedic treatment of dentition defects with metal-ceramic crowns and non-removable metal-ceramic bridges.\u0000Materials and methods. A total of 140 patients aged 34 to 49 years were examined during the work, who underwent the production of both single metal-ceramic crowns and non-removable metal-ceramic bridges. All patients were divided into 2 groups (70 patients each): the first one included patients with vital abutment teeth, the second one – with depulped. The abutment teeth had a different functional state – they were filled, had wedge-shaped defects or increased abrasion.\u0000Results. Orthopedic treatment showed that the manufacture of solid metal-ceramic crowns on dental teeth, which were filled more than 1 year ago, led to complications in 18 of 35 (51.4 %) patients in this group, which is insignificantly (P ˃ 0.05) by 17.2 % less than with similar prosthetics on depulped teeth. Regarding the manufacture of bridges, the trend of more complications in prosthetics on devital teeth persists, because in group I we had 20 (57.7 %) patients with complications, and in group II – 29 (82.9 %) cases, which is significantly different at the level of P < 0.05. In prosthetics of vital teeth, defects of hard tissues of which are replaced by filling material immediately before prosthetics, single crowns of complications occurred in 3 patients, i.e. in 8.6 %, which is insignificantly (P ˃ 0.05) less by 5.6 % than in group II where complications were found in 5 (14.2 %) people. Analysis of orthopedic treatment of patients with included defects of dentitions with metal-ceramic bridge prostheses revealed that when using filled teeth (filled for 1 year before prosthetics) in the case, when they were depulped, as abutment, complications were observed in 9 people, i.e. in 25.7 % of this group. This indicator insignificantly (P ˃ 0.05) exceeds the identical one from the first group (14.3 %) by 11.4 %, therefore, there is a tendency to reduce tooth resistance to negative external and internal factors, which has not acquired statistical significance.\u0000Conclusions. The study found that the frequency of complications that occur after prosthetics with fixed dentures depends on the condition of the tooth pulp, because the viable pulp provides metabolism, renewal of tooth cells and response to any damage. The need to replace fillings that were made more than 1 year ago to reduce the risk of secondary caries has been proven. Defects of hard tissues in the form of a wedge-shaped defect and the increased attrition do not increase frequency of complications in prosthetics by fixed dentures.","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"24 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79147743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The morphofunctional state of the fetal thyroid gland in maternal-fetal infections caused by Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae","authors":"P. S. Talapova, I. Sorokina","doi":"10.14739/2310-1237.2021.1.216292","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.216292","url":null,"abstract":"Aim – experimental modelling and determination of the morphofunctional state of the thyroid gland of a rat fetus in maternal-fetal infections caused by E. coli, S. aureus and K. pneumoniae.\u0000Material and methods. We conducted a controlled experimental study with block randomization on 85 female Wistar Albino Glaxo rats, which, depending on the group assigned randomly, were infected or not with E. coli, S. aureus and K. pneumoniae before pregnancy with subsequent pathomorphological examination of 37 thyroid glands obtained from rats’ fetuses. The set of methods was applied: histological – HE and Mallory staining; indirect immunofluorescence: moAb to T4, types I and III collagen, IL-6, and TNF; histomorphometry using ImageJ software. Statistical analysis was performed with the R environment and packages “ggplot”, “dplyr”, “pastecs”, “graphics” for Shapiro–Wilk test, Bartlett’s test, Box-Cox method, ANOVA, and plotting. The null hypothesis was rejected in cases when an error probability (P) did not exceed the type I error set at 0.001 (P < 0.001).\u0000Results. In the thyroid glands of fetuses obtained from mothers infected with E. coli, S. aureus and K. pneumoniae, in comparison with the control group, a statistically significant increase in the following morphofunctional parameters was determined: the diameter and area of the follicle, the area of the colloid, the height and area of the thyrocyte, the intensity and area of the fluorescence of the follicular thyrocytes and colloid, in samples labeled MoAb to T4, the intensity of the fluorescence of collagen types I and III; a decrease in the area of the nucleus in relation to the area of the cytoplasm, which was reflected in a decrease in the NCR index. The most pronounced differences in morphological and functional parameters from the norm were found in the thyroid gland of fetuses from mothers infected with S. aureus.\u0000Conclusions. The revealed changes in the morphofunctional parameters of the thyroid gland of a rat fetus, which experienced the influence of maternal-fetal infections caused by E. coli, S. aureus and K. pneumoniae, are unidirectional and statistically significantly different from those recorded during physiological organogenesis. They correspond to an increase in the gland’s secretory activity and indicate the acceleration of organ’s maturation.","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"45 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73985108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dependence of reactions of the external respiratory system in athletes on the orientation of the training process","authors":"S. M. Kanyhina, M. Potapenko, L. O. Kurilets","doi":"10.14739/2310-1237.2021.1.228920","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.228920","url":null,"abstract":"Issues of improving the functional training (different orientation of the training process) of athletes due the development of the respiratory system, including the respiratory system, as well as increasing the body's resistance to hypoxic stress remain extremely relevant at the present stage of sport development.\u0000The aim of the study is to investigate the peculiarities of the reaction of the external respiratory system in athletes, depending on the orientation of the training process.\u0000Materials and methods. The study involved 104 athletes (84 men and 20 women) skill level from CMS to IMS: 63 – athletes, who developed mainly endurance quality (triathletes, swimmers, long-distance runners, rowing), 31 – strength quality (weightlifters, powerlifting, weightlifting), 10 – speed quality (runners sprinters). The mean age of the athletes under study was 21.75 ± 3.32 years. Among them there are international masters of sports (IMS) – 2 athletes, masters of sports (MS) – 25, candidates for master of sports – 48, athletes of 1 degree – 29.\u0000Computer spirometric examination was performed for all athletes using the spirographic complex “Spirocom” produced by LLC “KHAI-Medica” in Kharkiv in accordance with existing recommendations.\u0000Research results. Analysis of computer spirometry showed, that athletes, who developed the quality of strength had 18.2 % (P = 0.014) greater vital capacity of the lungs, due to the predominance of respiratory volume by 29.9 % (P = 0.017) and by 42.1 % (P = 0.001) inspiratory reserve volume, but less by 22.4 % (P = 0.019) expiratory reserve volume, than athletes, who improved the quality of endurance. Athletes, who developed the quality of endurance in contrast to athletes, who improved the quality of speed had a higher forced vital capacity of the lungs by 14.9 % (P = 0.031), due to the predominance of a inspiratory reserve volume by 9.8 % (P = 0.049) and expiratory reserve volume by 28.5 % (P = 0.044). Athletes, who improved the quality of speed had lower vital capacity of the lungs by 26.1 % (P = 0.003), forced vital capacity of the lungs by 20.2 % (P = 0.016), due to a decrease of inspiratory reserve volume by 34.2 % (P = 0.008), than athletes, who improved the quality of strength. Athletes, who developed the quality of speed, had greater resistance to air flow in the bronchi of small caliber in the exhalation phase, as evidenced by a lower rate of forced expiratory volume1 by 21.1 % (P = 0.015), the volume of forced expiratory to reach the peak expiratory flow by 30.2 % (P = 0.016), MEF 75 by 22.9 % (P = 0.036), FEF 75–85 by 22.8 % (P = 0.035), than athletes, who developed the quality of strength.\u0000Conclusions Athletes, who have developed a quality of strength have a greater vital capacity of the lungs, due to the predominance of respiratory volume and inspiratory reserve volume, but less expiratory reserve volume, than in athletes, who improved the quality of endurance. Athletes, who developed endurance quality, in contrast to","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"82 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90121471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of clinical-neurological indicators of patients with hemorrhagic hemispheric stroke in the recovering period of the disease","authors":"С. О. Мєдвєдкова, А. О. Дронова","doi":"10.14739/2310-1237.2021.1.228923","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.228923","url":null,"abstract":"The aim of our study was the investigation of the dynamics of clinical-neurological indicators of patients with hemorrhagic hemispheric stroke (HHS) in the recovering period of the disease by comprehensive neurological, medical and social research.\u0000Materials and methods. 90 patients in the age from 38 to 70 years were studied, 58 men – 64.4 % and 32 women – 35.6 %. The condition of the patients was evaluated by NIHSS, mRS, BI and MSA on the 30th, 180th, 360th day of HHS. Depending on their self-care recovering level the patients were divided in two groups for analysis: the first one was formed with the patients whose self-care level completely was restored on the 360th day (ВІ = 100), and the second one was formed with the patients with BI 95 and less on the 360th day.\u0000Results. Structure of indicators according to NIHSS, mRS, ВІ and MSA on the 30th day of disease in these groups was analyzed during rehabilitation, as well as distribution of the patients based on their gender and process lateralization. Analyzing the data of the dynamics of the recovery period of HHS depending on the lateralization of the process and the sex of patients, we can notice that there was no significant difference during the recovery period of HHS depending of the lateralization of the process, for example, in patients with the damaged dominant hemisphere median score of BI was 70 points on the 30th day, patients with the lesion of subdominant hemisphere – 65, and on the 360th days in both groups it was 95 points. There was also no significant difference in the dynamics of neurological deficit and recovery of functional activity depending on the gender of patients. On the 30th day of the disease in the group of patients who fully restored their daily activity on the 360th day of HHS a significantly higher score for BI was determined than in the group with incomplete recovery (80 (70; 85) points against 55 (45; 65) points (P ˂ 0.05)). Although a significantly greater regression of functional disorders was found in the second group of patients (P ˂ 0.05). An analysis of the impact of spasticity on the recovery of self-care level showed a significant negative impact of increased muscle tone on the recovery of self-care (P ˂ 0.05) and reduction of the probability of complete recovery of independence in everyday life with increasing degree of spasticity (P ˂ 0.05). At the same time, no significant factors (sex, age, side of the lesion, etc.) were identified that would affect the development of spasticity in patients with HHS, except for the level of neurological deficit according to NIHSS. When we analyzed the distribution of patients in these two groups by sex, it was revealed that the groups didn't have any significant difference. There was also no significant difference in the study's groups depending of the lateralization of the stroke.\u0000Conclusions. It was found that on the 30th day of the disease in 35.6 % of patients the total score on NIHSS ranged from 2 to 6 points, ","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"4 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82817743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in expression of NLRP3 inflamasome and IL-1β in the development of oxazolone-induced colitis in rats and on the background of administration of simvastatin and interleukin-1β receptor antagonist","authors":"O. Zherebiatiev, N. Polishchuk","doi":"10.14739/2310-1237.2021.1.228928","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.228928","url":null,"abstract":"The aim. To study the expression of mRNA of the NLRP3 inflammasome and proinflammatory cytokine IL-1β in the inflamed colon of rats with experimental oxazolone-induced colitis and against the background of the introduction of simvastatin and antagonist of interleukin-1 receptors.\u0000Materials and methods. Experiments were carried out on male Wistar rats aged 8 months (body mass 120–150 g). Tissue samples were obtained from rats with experimental oxazolone-induced colitis (n = 20), rats with oxazolone-induced colitis treated with simvastatin (n = 20); rats with oxazolone-induced colitis treated with interleukin-1 receptor antagonist (n = 20) and control animals (n = 10). Clinical signs of colitis were evaluated by the clinical index of disease activity on the following parameters: weight loss, stool consistency and animal behavior. For macroscopic assessment of the development of colitis areas of inflammation and ulceration were investigated. For histological evaluation of lesions, sections of the intestine were stained with hematoxylin and eosin and cellular inflammatory infiltrates, epithelial hyperplasia, ulceration and loss of intestinal glands were studied. IL-1β and NLRP3 inflammasome mRNA expression was analyzed by real-time reverse transcriptase-polymerase chain reaction.\u0000Results. Animals treated with oxazolone rapidly developed colitis marked by weight loss and diarrhea peaking by day 2 after oxazolone administration and leading to death of 40 % of the rats by day 4. The histological observation showed inflammatory cell infiltration, including polymorphonuclear leukocytes and multiple erosive lesions in the large intestine. We determined that the expression level of the proinflammatory cytokine IL-1β is increased in colon samples of rats with oxazolone-induced colitis. IL-1β expression is increased 3.5‐fold in inflamed colon compared to uninflamed tissue. Administration of simvastatin and an interleukin-1 receptor antagonist to rats with oxazolone-induced colitis resulted in a 30 % and 2-fold decrease expression of IL-1β in colon samples. In addition, we examined NLRP3 expression in these tissues. RT‐PCR analysis demonstrates a 71‐fold increased expression of NLRP3 mRNA expression in colon samples tested. Administration of simvastatin and an interleukin-1 receptor antagonist to rats with oxazolone-induced colitis resulted in a 2.5- to 3.0-fold decrease expression of NLRP3 mRNA in colon samples.\u0000Conclusions. IL-1β and NLRP3 mRNA expression levels were elevated in the inflamed colon of rats with experimental oxazolone-induced colitis. Administration of simvastatin and an interleukin-1 receptor antagonist against the background of colitis caused a decrease in the expression levels of IL-1β and NLRP3 mRNA in the tissues of the intestine. Detection of abnormal expression of IL-1β and NLRP3 mRNA could provide insights into pathogenesis of Crohn's disease, and may help to identify future potential targets for therapeutic strategies in people with i","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"15 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78372396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of serum S100B concentration in patients with acute spontaneous supratentorial intracerebral hemorrhage","authors":"О. A. Koziolkin, A. Kuznietsov","doi":"10.14739/2310-1237.2021.1.228850","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.1.228850","url":null,"abstract":"Aim of the work – to evaluate the informativeness of serum protein S100B levels in detection of short-term prognosis in patients with acute period of spontaneous supratentorial intracerebral hemorrhage (SSICH) on the background of concervative therapy.\u0000Materials and methods. Prospective cohort study of 90 patients with acute SSICH on the background of conservative therapy was done. Level of neurological deficit was evaluated using Full Outline of Unresponsiveness Scale, Glasgow Coma Scale and National Institute of Health Stroke Scale. Computed tomography was done to detect the intracerebral hemorrhage volume (ICHV), secondary intraventricular hemorrhage volume (SIVHV) and total volume of intracranial hemorrhage (TVICH). Laboratory blood samples were taken within 24 hours of hospitalization. Levels of S100B protein in serum were measured using immunoassay analysis. Unfavorable variants of course (early neurological deterioration (END) during 48 hours from hospitalization) and disease acute period outcome of ICH (modified Rankin score 4–6 on the 21st day of the disease) were considered as endpoints. Statistical processing of the obtained results included correlation analysis, logistic regression analysis and ROC-analysis.\u0000Results. Neurological deterioration during 48 hours after hospitalization was detected in 18 (20.0 %) patients, unfavorable SSICH acute period outcome was revealed in 49 (54.4 %) patients. It was detected that serum S100B protein correlates with ICHV (R = 0.34, P ˂ 0.01), SIVHV (R = 0.39, P ˂ 0.01) and TVICH (R = 0.45, P ˂ 0.01). Thus, the patients with unfavorable SSICH acute period course and outcome are characterized with the higher S100B protein levels (P ˂ 0.0001). High sensitive multipredicive logistic regression model that integrates prognostic value of serum of S100B protein concentration with informativeness of clinical, neurological parameters (National Institute of Health Stroke Scale score, SIVHV) was elaborated. It helps to detect the individual risk of unfavorable acute SSICH period outcome on the ground of conservative therapy with the accuracy 90.0 % (AUC ± SE (95 % CІ) = 0.95 ± 0.02 (0.89–0.99), P ˂ 0.0001).\u0000Conclusions. Serum S100B protein levels in patients with SSICH on the 1st day after hospitalization is the informative additional parameter in verification of short-term prognosis on the background of conservative therapy.","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"35 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79496112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The risk of development of acute kidney injury in full-term infants with administration of methylxanthines","authors":"L. Stryzhak, I. Anikin, O. Spakhi","doi":"10.14739/2310-1237.2021.2.230342","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.2.230342","url":null,"abstract":"Exploring new possibilities for the use of methylxanthines to prevent the development of acute kidney injury (AKI) in full-term infants with perinatal asphyxia. Aim: to evaluate the efficacy and safety of methylxanthines in full-term infants for the prevention and conservative treatment of acute kidney injury. Materials and methods. To test the effectiveness of the proposed method of AKI treatment, 38 infants were chosen and divided into 2 groups by random selection. Nursing and intensive care were according to current legislation (Order of the Ministry of Health of Ukraine No. 225 of March 28, 2014). The main group (n = 20) received therapy with caffeine citrate, the comparison group (n = 18) – theophylline. Both of these drugs were used to prevent the development of acute kidney injury – stage II and III according to KDIGO. Results. A significant difference in serum creatinine was found in the main group - the level of serum creatinine was higher than in the comparison group, but did not exceed the physiological norm. GFR on the 3rd day of life was higher with administration of theophylline, but in the group of caffeine did not exceed the reference values of the norm. No differences between urea levels and diuresis rates were found in the groups. The initial results indicate the lack of statistical significance when using various drugs of the methylxanthine group, namely theophylline and caffeine citrate. This is explained by the fact that in the main group 65.00 % (n = 13) of patients had AKI stage 0 according to KDIGO, and 35.00 % (n = 7) had stage I. In the comparison group, 55.56 % (n = 10) and 44.44 % (n = 8), respectively. Stages II and III in both groups of the study did not develop, the obtained data are equivalent – U = 163,00; P = 0,6296. However, the use of caffeine citrate may become a priority due to a better safety profile compared to theophylline. Caffeine is less likely to cause adverse effects in the form of non-pathological bile vomiting and has significantly lower relative risk of non-pathological bile vomiting in infants (RR 0.26 (95 % CI 0.10; 0.66)). Conclusions. Conservative methylxanthine therapy in full-term infants with perinatal asphyxia prevents the development of stages II and III of AKI according to KDIGO. However, it is necessary to continue the collection of material to increase the statistical significance, as well as to study the early and long-term consequences of this therapy.","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"39 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72723236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between morphological indicators of the tibia and soft tissues lesions and some biochemical blood indicators in patients with traumatic osteomyelitis","authors":"V. Hryhorovskyi, S. Mahomedov, M. Hrytsai, V. Tsokalo, T. A. Kuzub, L. Polishchuk, A. Hryhorovska","doi":"10.14739/2310-1237.2021.2.237156","DOIUrl":"https://doi.org/10.14739/2310-1237.2021.2.237156","url":null,"abstract":"Aim. Research aim was to find statistical differences of biochemical indicators of blood and morphometric indicators of crural tissue lesion and relationships between them in patients with traumatic osteomyelitis of the tibia with trophic disturbances in extremity. Material and methods. The work material includes data from 28 patients with traumatic osteomyelitis which was combined with trophic tissue disturbances of different manifestation degree. The levels of procalcitonin, lactate dehydrogenase and acetylcholinesterase of blood serum were estimated. In crural tissues quantitative biochemical, gradual morphometric indicators, frequencies of occurrence of different manifestation degree cases and relationships “clinic – biochemistry – pathomorphology” were defined histologically. Results. Average values of procalcitonin, lactate dehydrogenase and acetylcholinesterase in patients with traumatic osteomyelitis of the tibia, combined with trophic disturbances in crural tissues, are significantly higher, than average values of these indicators in intact donors. In the majority of the patients with chronic traumatic osteomyelitis in crural tissues pathological changes were found out, which by clinical estimations are considered as trophic disturbances. They represent a combination of discirculatory, ischemic-necro­tic, exsudative, productive-inflammatory, and also reparative processes which in total create a composite picture of complex lesion and can persist for many years. As closest relations, according to values of association coefficient, concerning certain biochemical indicators, can be noted such clinical indicators: “degree of bone fragments non-consolidation” and “severity of trophic disturbances in bone tissue (by clinical estimation)”, concerning defined morphometric indicators – such biochemical indicators: “level of procalcitonin” and “level of lactate dehydrogenase” in blood serum. Among morphometric lesion indicators the closest relations concerning biochemical indicators were revealed: in bones – “the form of the pathological focus of traumatic osteomyelitis” and “presence of sequesters”, in soft tissues – “exsudative inflammation in internal membrane of a focus capsule”. Conclusions. In chronic traumatic osteomyelitis combined with trophic disturbances of crural tissues, there are relationships between some clinical, biochemical and morphological indicators of the extremity tissues condition. A deviation of metabolism indicators from norm can serve as biochemical markers in definition of severity degree and efficiency of treatment in patients.","PeriodicalId":41645,"journal":{"name":"Pathologia","volume":"150 1","pages":""},"PeriodicalIF":0.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77401300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}