Alena Abaimova, N. N. Kartsev, Edvard Svetoch, O. I. Tazina, T. Novikova, M. Platonov, I. Mitsevich, M. Kanashenko, Rostislav Zhumakaev, K. Detushev, M. G. Teymurazov
{"title":"Mundticin KS: Characterization and Production Method","authors":"Alena Abaimova, N. N. Kartsev, Edvard Svetoch, O. I. Tazina, T. Novikova, M. Platonov, I. Mitsevich, M. Kanashenko, Rostislav Zhumakaev, K. Detushev, M. G. Teymurazov","doi":"10.12691/JAEM-9-1-3","DOIUrl":"https://doi.org/10.12691/JAEM-9-1-3","url":null,"abstract":"The paper aims to develop a method for the single-stage purification of mundticin KS and to study its physicochemical and antimicrobial properties. Mundticin produced by strain Enterococcus mundtii B-8398 and belonging to class IIa bacteriocins was obtained by adding 10% of the CM Sephadex C-25 sorbent to the culture medium before cultivation. At the end of cultivation, the sorbent was collected and packed into a column followed by a one-step elution. As a result, a fraction containing mundticin KS was obtained with a purification quality of about 70%. The activity of mundticin KS remained stable over a wide range of pH and temperatures. It is completely inactivated by such enzymes as proteinase K and α-chymotrypsin and partially by trypsin; however, such enzymes as amylase, lipase, and papain do not have any effect on its activity. Mass spectral analysis, electrophoresis, and DNA sequencing showed the high range similarity of mundticin KS produced by strains E. mundtii B-8398 to other previously studied mundticins. The antibacterial activity of mundticin KS was recorded ageist all tested strains of Listeria spp., Enterococcus spp., and Clostridium perfringens in nanomolar concentrations. In addition, mundticin KS inhibits the growth of most tested Gram-positive food-borne pathogens. By the sorbent cultivation method, it was possible to increase the yield of mundticin KS by 2,9 times compared to the control.","PeriodicalId":415791,"journal":{"name":"Journal of Applied & Environmental Microbiology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117244566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hydrolytic and Inhibitory Activity of Two Closely Related Bacillus Isolates","authors":"O. Akintunde, Chukwunonso Chukwudozie","doi":"10.12691/JAEM-9-1-2","DOIUrl":"https://doi.org/10.12691/JAEM-9-1-2","url":null,"abstract":"Two Bacillus strains with different colony morphology were isolated from the soil. Phylogeny and 16S rRNA analysis reveals they both belong to the Bacillus subtilis group with both strains showing close relatedness to B. subtilis strain 168 and other related strains. These two isolates were examined for cellulase, amylase and lipase activity. Hydrolytic activity was measured via relative enzyme activity. Both strains showed promising results with substrate utilization and Bacillus sp strain SI3 displayed a better amylolytic, cellulolytic and lipolytic activity. Proteolytic ability was also assessed using skimmed milk agar and gelatin. Furthermore, the culture extracts of both isolates were examined for antimicrobial activity. This work demonstrates that these strains could have potential application in the industrial production of these economically important enzymes and other bioactive compounds.","PeriodicalId":415791,"journal":{"name":"Journal of Applied & Environmental Microbiology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134080728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Technological and Probiotic Abilities of Local Lactic Acid Bacteria","authors":"Lesly Samedi, A. L. Charles","doi":"10.12691/JAEM-7-1-3","DOIUrl":"https://doi.org/10.12691/JAEM-7-1-3","url":null,"abstract":"The definition of probiotics has been the topic of much discussion and the most recent definition from FAO/WHO stipulates that probiotics are “live microorganisms, administered in adequate amounts, confer a health benefit on the host”. Dairy products remain one of the most important sources of lactic acid bacteria. Today, these kinds of bacteria are of increasing interest as they are considered functional foods when combined with lactic acid bacteria. The identification and classification of isolates made difficult the research, since the benefits should only be pertinent to specific isolates. However, bacteria strains have a certain number of potential and well-established benefits. They may play a role in preventing and treating diarrhea and act on the immune system, improve lactose digestion, help the body to resist, and fight infection. Further researches need to be conducted to confirm the roles that lactic acid bacteria may play in antitumor effects, hyper cholesterol effects, preventing urogenital infections, alleviating constipation, and treating food allergies. In addition to food shelf-life and safety, consumers are showing interest in the relationship and bioactive roles of “functional foods’ in preventing or managing non-transmissible chronic diseases. Since then, increased demand for non-dairy probiotic products has come from vegetarianism, milk cholesterol content, and lactose intolerance. Therefore, the establishment of the probiotic functional characteristics of isolated strains must be a key factor in the search for probiotic microorganisms and their inclusion in the food product design. This review presents a basic overview of the evaluation of technological and probiotic abilities of lactic acid bacteria strains and the determination of their probiotic properties.","PeriodicalId":415791,"journal":{"name":"Journal of Applied & Environmental Microbiology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115056717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Raheel, A. Orabi, Saber El-Hanbaly, Hassan Shaheen
{"title":"Action Mechanism and Immunity Status of Broiler Chickens after Spray and Drinking Water Administration of Kanne-Brottrunk®","authors":"I. Raheel, A. Orabi, Saber El-Hanbaly, Hassan Shaheen","doi":"10.12691/jaem-6-3-1","DOIUrl":"https://doi.org/10.12691/jaem-6-3-1","url":null,"abstract":"Poultry producers are challenged to improve production while using fewer antibiotics due to increased restriction on antimicrobial usage so prebiotics and probiotics are being tested under different experimental conditions to study the pathways used by these substances to assist in the prevention of carcass contamination and in the elimination of pathogens present in the bird's organisms. The current study aimed to investigate the effect of spray and orally administered Kanne-Brottrunk® which contain Lactobacillus and Bifidobacterium at which lactic acids bacteria concentration is 5x106 cfu/ml in addition to vitamins, minerals, essential amino acids and trace elements on the mucosal trachea IgA, IL-6 and INF-γ as well as healthiness of intestinal and tracheal mucosa using electron microscopy. Six hundred one day old Cobb broilers chickens were reared on litter in three separated groups each one contain 200 chicks, 1st group \"Drinking Water Administrated\", 2ndgroup \"Spray Administrated\" and \"control non Kanne-Brottrunk® medicated group\". Serum, trachea and intestine collected for evaluation of immune status and epithelium healthiness at 14, 21, 28 and 35 day old. Immunological Parameters measured were IL-6, INF-γ and Mucosal IgA concentration using EISA kits accompanied by tracheal and intestinal electron microscopy histomorphology. The results proved that Kanne-Brottrunk® administrated in water stimulate immune response by increased level of IL-6, INF-γ and Mucosal IgA and also increase immune cells infiltration as plasma cell, lymphoid cells, macrophages, and heterophile in trachea and intestine with protection of their tracheal cilia and intestinal villi more than aerosol administration.","PeriodicalId":415791,"journal":{"name":"Journal of Applied & Environmental Microbiology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132434249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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