Action Mechanism and Immunity Status of Broiler Chickens after Spray and Drinking Water Administration of Kanne-Brottrunk®

Abstract

Poultry producers are challenged to improve production while using fewer antibiotics due to increased restriction on antimicrobial usage so prebiotics and probiotics are being tested under different experimental conditions to study the pathways used by these substances to assist in the prevention of carcass contamination and in the elimination of pathogens present in the bird's organisms. The current study aimed to investigate the effect of spray and orally administered Kanne-Brottrunk® which contain Lactobacillus and Bifidobacterium at which lactic acids bacteria concentration is 5x106 cfu/ml in addition to vitamins, minerals, essential amino acids and trace elements on the mucosal trachea IgA, IL-6 and INF-γ as well as healthiness of intestinal and tracheal mucosa using electron microscopy. Six hundred one day old Cobb broilers chickens were reared on litter in three separated groups each one contain 200 chicks, 1st group "Drinking Water Administrated", 2ndgroup "Spray Administrated" and "control non Kanne-Brottrunk® medicated group". Serum, trachea and intestine collected for evaluation of immune status and epithelium healthiness at 14, 21, 28 and 35 day old. Immunological Parameters measured were IL-6, INF-γ and Mucosal IgA concentration using EISA kits accompanied by tracheal and intestinal electron microscopy histomorphology. The results proved that Kanne-Brottrunk® administrated in water stimulate immune response by increased level of IL-6, INF-γ and Mucosal IgA and also increase immune cells infiltration as plasma cell, lymphoid cells, macrophages, and heterophile in trachea and intestine with protection of their tracheal cilia and intestinal villi more than aerosol administration.