Exploration of BioMat-X最新文献

{"title":"Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing","authors":"Shujing Li, Wensheng Pan, Maohu Chen, Ruyue Wang, Feng Chen","doi":"10.37349/ebmx.2025.101339","DOIUrl":"https://doi.org/10.37349/ebmx.2025.101339","url":null,"abstract":"Aim: Acute cutaneous injuries and refractory chronic wounds represent prevalent clinical challenges in daily life. To address the impediments to wound healing, we propose a novel hydrogel-based therapeutic approach designed to prevent bacterial invasion, mitigate infection-induced persistent inflammatory responses, and reduce excessive oxidative stress, thereby enhancing the wound healing process. Methods: This study presents a method for preparing hyaluronic acid/silk fibroin (HA/SF) composite hydrogels via photo-crosslinking. HA and SF were respectively modified via methacrylation and glycidyl methacrylate to synthesize HAMA and SFMA. Mussel-inspired catechol groups were then grafted onto HAMA chain segments to prepare precursor HAC. Under photo-initiator LAP, polymerization was triggered to ultimately form a hydrogel network integrating mechanical toughness and tissue adhesiveness. Composite hydrogels with varying degrees of crosslinking are synthesized by adjusting the SFMA content. Results: The results demonstrate that this hydrogel can effectively achieve hemostasis within 20 seconds. Lap shear testing revealed that the HASF-gel-2 hydrogel exhibited the highest maximum adhesive strength of 160.3 kPa among all experimental groups. Furthermore, while the cell viability of the control group was normalized to 1, the composite hydrogel groups displayed values of 1.015, 1.085, 1.136, and 1.263, respectively, indicating favorable biocompatibility. The appropriate incorporation of SF was shown to enhance cellular proliferation. On day 3 post-wounding, the HASF hydrogel group demonstrated a wound closure rate of 41.7%, outperforming commercial products under identical conditions. Conclusions: In rat wound models, the HASF composite hydrogel significantly accelerated wound healing progression.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.explorationpub.com/uploads/Article/A101339/101339.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of nanoscale titanium-porphyrin coordination nanomaterials for photodynamic-sonodynamic synergistic therapy of tumor cells","authors":"Wei Wang, Wei-Jia Huang, Liu Huang, Yan Li, Shuzhang Xiao, Haichuang Lan, Peng Geng","doi":"10.37349/ebmx.2025.101337","DOIUrl":"https://doi.org/10.37349/ebmx.2025.101337","url":null,"abstract":"Aim: Multifunctional nanomaterials with photodynamic-sonodynamic therapy (PSDT) potential offer significant advantages in cancer treatment. However, designing and preparing single-component “two-in-one” multifunctional nanomaterials remains challenging. Hematoporphyrin monomethyl ether (HMME), a second-generation porphyrin-related sonosensitizer, is a porphyrin derivative with two asymmetric carboxyl groups. Notably, the carboxyl groups in HMME can coordinate with metal ions to construct metal-organic coordination nanomaterials (MCPs). Titanium (Ti), a biocompatible metal element, is commonly used in medical devices such as implantable metal alloys. Therefore, this study reported the synthesis of “two-in-one” type Ti-HMME coordination nanomaterials (TiCPs) as efficient nanoscale photo/sonosensitizers. Methods: Under a nitrogen atmosphere, TiCPs were synthesized via self-assembly between HMME and Ti4+ ions. Results: The average particle size of TiCPs was approximately 70 nm. Additionally, TiCPs contained the photo/sonosensitizer HMME, which could convert O2 into cytotoxic reactive oxygen species (ROS) under light and ultrasound (US) excitation. The generation of ROS could be detected using 1,3-diphenylisobenzofuran (DPBF). When the mixed solution (TiCPs + DPBF) was irradiated with light, the DPBF peak rapidly decreased with increasing irradiation time, indicating the production of ROS by TiCPs under light. Similarly, the absorbance of TiCPs + DPBF significantly decreased with increasing US time, demonstrating the sonodynamic effect of TiCPs + US. After 10 min of light or US excitation, 49.4% (Light) and 38.1% (US) of DPBF were oxidized by ROS generated by TiCPs, showcasing excellent photodynamic/sonodynamic effects. In vitro cell experiments further demonstrated that TiCPs had excellent biocompatibility, could be effectively internalized by cells, and significantly reduced cell viability under light and US excitation, effectively killing tumor cells. Conclusions: This study not only demonstrated TiCPs as “two-in-one” type multifunctional nanomaterials for PSDT but also provided insights into designing other photo/sonosensitizer molecules with similar HMME structures for tumor theranostics.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.explorationpub.com/uploads/Article/A101337/101337.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials in dentistry: the analogue/digital transition","authors":"L. Fiorillo","doi":"10.37349/ebmx.2024.00012","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00012","url":null,"abstract":"","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141266287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue engineering approach to the creation of grafting material for rhinoplasty: clinical сase reports","authors":"Vladimir Karpiuk, I. Gilevich, Marina Perova, O. Ponkina","doi":"10.37349/ebmx.2024.00011","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00011","url":null,"abstract":"The use of autologous cartilage and bone grafts remains the gold standard in augmentation rhinoplasty performed to reconstruct of the nasal dorsum. Meanwhile, limited number of available sources, donor site morbidity, and unpredictable graft resorption represent significant disadvantages of autografting. The aim of this study is to test combination of autologous stromal vascular fraction (SVF) and commercially available bone substitutes (BSs) as new tissue-engineered grafting material (GM) for rhinoplasty. A series of consecutive cases includes four adult patients who underwent rhinoplasty to correct saddle nose deformity (SND) using the new graft technique. SVF was isolated from liposuction aspirate using standard methodology of enzymatic digestion. Two types of BSs were combined with SVF: Bio-Oss granules to create a moldable graft (M-graft), and block-shaped BoneMedik-S to create rigid grafts (R-grafts). The moderate SND was treated using an M-graft. In case of major or complex SND, the nasal dorsum was reconstructed with dorso-columellar L-shaped framework made of R-grafts. The results were evaluated over a period of 6 months to 3 years postoperatively using photogrammetry and FACE-Q appearance appraisal scales. Computerised tomography (CT) scanning of the reconstructed nose and histological analysis of grafted material were also carried out. No complications were observed. The photograms show the restoration of the correct contour of the nose. FACE-Q appraisal scale scores increased significantly, including satisfaction with nose appearance, psychological well-being, and social function. In CT evaluation, there was no substantial resorption or warping of the grafts. Histological findings show osteogenic remodeling of the grafted material. Thus, combining autologous SVF with BSs is a promising strategy for developing rhinoplasty GM.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141271674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of circulating small extracellular vesicles in neurodegenerative disease through targeted biomarkers and advancements in biosensing","authors":"Saqer Al Abdullah, Ivy Cocklereece, Kristen Dellinger","doi":"10.37349/ebmx.2024.00008","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00008","url":null,"abstract":"Neurodegenerative diseases (NDDs) gradually affect neurons impacting both their function and structure, and they afflict millions worldwide. Detecting these conditions before symptoms arise is crucial for better prognosis and duality of life, given that the disease processes often begin years earlier. Yet, reliable and affordable methods to diagnose NDDs in these stages are currently lacking. There’s a growing interest in using circulating extracellular vesicles (EVs), like small EVs (sEVs) also known as exosomes, as potential sources of markers for screening, diagnosing, and monitoring NDDs. This interest stems from evidence showing that these EVs can carry brain pathological proteins implicated in NDD pathology, and they can even traverse the blood-brain barrier. This review focuses on the creation of EVs, particularly sEVs with a size of less than 200 nanometers, methods for isolating sEVs, and recent advancements in biosensor development to detect NDD-related markers found in sEVs. Furthermore, it explores the potential of sEVs in diagnosing four major NDDs: Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and multiple system atrophy (MSA).","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"50 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140662289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A biomimetic approach to modulating the sustained release of fibroblast growth factor 2 from fibrin microthread scaffolds","authors":"Meagan E. Carnes, Cailin R. Gonyea, Jeannine M. Coburn, G. Pins","doi":"10.37349/ebmx.2024.00006","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00006","url":null,"abstract":"Aim: The pleiotropic effect of fibroblast growth factor 2 (FGF2) on promoting myogenesis, angiogenesis, and innervation makes it an ideal growth factor for treating volumetric muscle loss (VML) injuries. While an initial delivery of FGF2 has demonstrated enhanced regenerative potential, the sustained delivery of FGF2 from scaffolds with robust structural properties as well as biophysical and biochemical signaling cues has yet to be explored for treating VML. The goal of this study is to develop an instructive fibrin microthread scaffold with intrinsic topographic alignment cues as well as regenerative signaling cues and a physiologically relevant, sustained release of FGF2 to direct myogenesis and ultimately enhance functional muscle regeneration.\u0000Methods: Heparin was passively adsorbed or carbodiimide-conjugated to microthreads, creating a biomimetic binding strategy, mimicking FGF2 sequestration in the extracellular matrix (ECM). It was also evaluated whether FGF2 incorporated into fibrin microthreads would yield sustained release. It was hypothesized that heparin-conjugated and co-incorporated (co-inc) fibrin microthreads would facilitate sustained release of FGF2 from the scaffold and enhance in vitro myoblast proliferation and outgrowth.\u0000Results: Toluidine blue staining and Fourier transform infrared spectroscopy confirmed that carbodiimide-conjugated heparin bound to fibrin microthreads in a dose-dependent manner. Release kinetics revealed that heparin-conjugated fibrin microthreads exhibited sustained release of FGF2 over a period of one week. An in vitro assay demonstrated that FGF2 released from microthreads remained bioactive, stimulating myoblast proliferation over four days. Finally, a cellular outgrowth assay suggests that FGF2 promotes increased outgrowth onto microthreads.\u0000Conclusions: It was anticipated that the combined effects of fibrin microthread structural properties, topographic alignment cues, and FGF2 release profiles will facilitate the fabrication of a biomimetic scaffold that enhances the regeneration of functional muscle tissue for the treatment of VML injuries.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"48 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140666351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cold plasma treatment on the biological performances of decellularized bovine pericardium extracellular matrix-based films for biomedical applications","authors":"Maria Elena Lombardo, Valentina Mariscotti, P. Chevallier, F. Copes, Francesca Boccafoschi, A. Sarkissian, Diego Mantovani","doi":"10.37349/ebmx.2024.00007","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00007","url":null,"abstract":"Aim: Since decades, decellularized extracellular matrix (dECM)-derived materials have received worldwide attention as promising biomaterials for tissue engineering and biomedical applications. Soluble dECM is a versatile raw material that can be easily engineered into the desired shapes and structures. However, there are still some limitations restricting its use, including low hydrophilicity and smooth surfaces, which negatively influence cell adhesion/spreading. The objective of the present study was to investigate surface modification by nitrogen/hydrogen (N2/H2) low-pressure cold plasma treatment as a potential technique to improve the biological response of bovine pericardium dECM films.\u0000Methods: Bovine pericardium dECM was enzymatically digested and lyophilized prior to the preparation of thin films via solvent-casting method. Changes in surface properties after plasma treatment were investigated using water contact angle (WCA) and X-ray photoelectron spectroscopy (XPS) measurements. Immunofluorescence staining and resazurin assay for human dermal fibroblasts (HDFs) cultured on the dECM films were used to assess the bioactivity of dECM films. Finally, the hemocompatibility of the films was investigated via clotting time and hemolysis assay.\u0000Results: WCA and XPS results revealed that oxygen (O)- and N-containing functional groups were incorporated onto the film surface and an increase in hydrophilicity was observed after plasma treatment. In vitro experiments showed that cell adhesion in plasma-treated dECM films is much faster if compared to the untreated controls. Moreover, the fibroblast proliferation increased after plasma surface modifications. Finally, the hemocompatibility analysis results indicated a delayed blood clotting and no hemolytic effects for all the tested samples.\u0000Conclusions: These findings confirmed the potential of dECM as raw material for biocompatible thin films fabrication. Additionally, plasma surface treatment emerged as an eco-friendly and cost-effective strategy to enhance in vitro cell attachment and proliferation on dECM films, expanding their applications in biomedicine.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"32 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140671457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of pHEMA hydrogel conduit containing GelMA-HaMA IPN for peripheral nerve regeneration","authors":"Damla Arslantunali Sahin, C. D. Son, V. Hasırcı","doi":"10.37349/ebmx.2024.00005","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00005","url":null,"abstract":"Aim: Small defects after any injury to the periperal nerves results in self-regeneration. However, for larger defects, suturing or grafting are necessary, which may have limitations. Thus, research on nerve guidence conduits is needed without drawbacks. The aim of the study was to develop hydrogel-based conduits containing interpenetrating network (IPN).\u0000Methods: Methacrylated gelatin (GelMA)-methacrylated hyaluronic acid (HaMA) IPN was filled the poly(2-hydroxyethylmethacrylate) (pHEMA) the outer conduit. Schwann cells (SCs) were used on the pHEMA and the distal end of the tube was injected with netrin-1 to support model SH-SY5Y cells.\u0000Results: 1H-nuclear magnetic resonance (1H-NMR) showed that methacrylation degrees were 94% ± 2% for GelMA and 60% ± 7% for HaMA. The fraction of HaMA increased the degradation rate; pure HaMA degraded in 3 weeks, while pure GelMA in more than 5 weeks. An increase in the fraction of 2-hydroxyethylmethacrylate (HEMA) from 20% to 56% decreased the porosity and the pore size, significantly. SH-SY5Y cells migrated along the conduit in the presence of netrin-1. NeuN expression was increased in 2 weeks indicating neuronal activity.\u0000Conclusions: SH-SY5Y cells produced neurites in the IPN. pHEMA conduit including GelMA-HaMA IPN is a good candidate for peripheral nerve regeneration applications. As future studies, the conduit will be tested in vivo for nerve regeneration.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"5 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Journey of medical device development from the bench to the bedside—four real-life examples of commercial biomaterials development","authors":"Yves Bayon, Christophe Bureau, Maria J N Perreira, François Aubert, Michel Caillibotte, R. Vestberg, Didier Letourneur","doi":"10.37349/ebmx.2024.00004","DOIUrl":"https://doi.org/10.37349/ebmx.2024.00004","url":null,"abstract":"Translating biomaterials research into clinical products is a multidisciplinary yet rewarding journey. It should primarily aim to improve patient treatment and clinical outcomes by addressing unmet clinical needs. Four examples of commercial development of biomaterial implants illustrate the diversity of paths, starting from academic research work (AlchiMedics, TISSIUM, Cousin Surgery) or corporate initiatives to develop new products (Medtronic-Sofradim Production). They have been selected from the Translational Research session of the 2022 Conference of the European Society for Biomaterials (ESB) in Bordeaux (France). Commitment, agility, and perseverance were among the key common skills to successfully meet challenges, especially the most unexpected ones. All dimensions of translation projects must be integrated from the start, including the regulatory strategy.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of anthracite-derived multicolor graphene quantum dots for their potential application in nanomedicine","authors":"Hongyu Pan, Jiahe Chen, Weifeng Zhang, Liuhao Chen, Ji Young Yoon, Bin Li, Kai Yu","doi":"10.37349/ebmx.2023.00003","DOIUrl":"https://doi.org/10.37349/ebmx.2023.00003","url":null,"abstract":"Aim: This study aims to discover an alternative precursor with abundant source and low cost for multicolor graphene quantum dots (GQDs) preparation and application.\u0000Methods: In the current study, anthracite-derived multicolor GQDs were prepared at different reaction temperatures (100°–150°C), referring to the GQDs 100, GQDs 120, GQDs 130, and GQDs 150.\u0000Results: The GQDs 100, GQDs 120, GQDs 130, and GQDs 150 solutions were found to be orange-red, yellow-green, green, and blue under 365 nm excitation UV (ultraviolet) lamp, respectively. The X-ray photoelectron spectroscopy (XPS) data suggests high temperature intensifies oxidation of the amorphous sp3 carbon, resulting in GQDs with higher crystalline structure (Csp2). Compared with the GQDs 100 and GQDs 120, the GQDs 130 and GQDs 150 showed much better biocompatibility, which may attribute to their higher Csp2 composition and smaller size.\u0000Conclusions: The results suggest that GQDs 130 and GQDs 150 are ideal candidates for nanomedicine applications, e.g., drug/gene delivery and bio-imaging, etc.","PeriodicalId":411664,"journal":{"name":"Exploration of BioMat-X","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138594448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}