Annales Bogorienses-Journal of Tropical General Botany最新文献

{"title":"Identification Of Aquatic Plant Species From Bantimurung Waterfall And Their Phytochemical Compounds Analysis","authors":"M. Nugraha, Fasya Hadaina Maharani, H. Novita, M. A. Rajamuddin, R. Yunita, R. Reflinur, W. Enggarini, A. Julzarika, B. Elya","doi":"10.14203/ANN.BOGOR.2019.V23.N1.41-48","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.41-48","url":null,"abstract":"A total of 21 aquatic plant species were collected from Bantimurung waterfall areas, South Sulawesi, Indonesia, in October 2017. These plant materials were subjected to both species and phytochemicals identification. The aims of this study were to determine the species or taxonomic rank of Indonesian aquatic plants collected from Bantimurung waterfall, South Sulawesi, Indonesia and to identify their chemical compounds (phytochemicals) as a candidate for new herbal medicine. Plant genetic materials used in this study were collected from Bantimurung Bulusaraung waterfall and were then identified based on standard botanical techniques for species identification in the Herbarium Bogoriense, Research center for Biology Indonesian Institute of Science (LIPI), Cibinong, West Java. The samples were subjected to the phytochemistry screening such as alkaloids, flavonoids, tannins, saponins, glycosides, terpenoids and anthraquinone followed the procedures of Indonesian Materia Medika and Harborne.  Results showed that all collected aquatic plant samples were able to be identified, including their species names. Phytochemical screening of each sample revealed the presence of glycoside in all of the tested species. However, no alkaloids, anthraquinones, and terpenoids were observed in those tested plant samples. Of the total 21 aquatic plants, 14 species contained flavonoids, 8 species contained phenol compound, and 10 species contained saponins. Among these species Donnax canniformis possessed good antioxidant activity, which correlated to its total phenolic and flavonoid contents. Our results would be beneficial for any future effort in the development of new herbal drugs derived from aquatic plants.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86695899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty","authors":"S. Rahmawati, Chairunnisa Chairunnisa, E. Erdayani, S. Nugroho, A. Estiati","doi":"10.14203/ANN.BOGOR.2019.V23.N1.30-40","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.30-40","url":null,"abstract":"OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains  also increased significantly (~16-22%).  After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress.  Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86871096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia","authors":"A. E. Susanti, S. Ratnakomala, W. Mangunwardoyo, P. Lisdiyanti","doi":"10.14203/ANN.BOGOR.2019.V23.N1.1-12","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.1-12","url":null,"abstract":"    Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"238 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76111623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme Production From Cassava Peels by Aspergillus Awamori KT-11: The Making of Natural Sweetener From Several Tubbers","authors":"R. Melliawati, F. Rahman","doi":"10.14203/ANN.BOGOR.2019.V23.N1.20-29","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.20-29","url":null,"abstract":"The use of cassava (Manihot esculenta Crantz) peel for enzyme production has not been widely used. The purpose of this study was to produce complex amylase enzymes from cassava peel by A. awamori KT-11 and apply them in the manufacture of natural sweeteners. Enzyme production is carried out on red and white cassava peel. Media of cassava peel sterilized, inoculated with 1%  A. awamori KT-11, incubated for 5 days, then dried at 50°C and mashed. Making sugar is done on cassava flour, sweet potato ( Ipomoea batatas L), taro (Colocasia esculenta) and cocoyam (Xanthosoma sagittifolium) with different concentrations of 10%, 15%, 20%, and 15% and 20% enzyme concentrations. The hydrolysis process is carried out for 3 days at 60°C. The enzyme activity in red cassava peel was 405,006 U/mL and white cassava peel was 321,239 U/ml. The sugar produced in cassava, taro, sweet potato, and Cocoyam was 101.38 mg/mL, 81.18 mg/mL, 55.929 mg/mL, and 42.874 mg/mL, respectively. The results of TLC showed that cassava and taro sugar  contain maltose, lactose and glucose, sweet potatoes contained glucose and dextrin and Cocoyam containing fructose. The sweetness level of sugar from cassava, taro, sweet potato and Cocoyam is 14 brix, 12 brix, 9 brix and 9 brix, respectively.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77369804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Increased Fruit Content of Soluble Proteins, Sugars, Certain Enzyme Activities, and Certain Polyphenols in Context to Date Fruit Ripening","authors":"S.S.M. Alqarni, M. Bazzi","doi":"10.14203/ANN.BOGOR.2019.V23.N1.49-57","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.49-57","url":null,"abstract":"Introduction: To understand the increased fruit content of soluble proteins, sugars, certain enzymes activities, and certain polyphenols in context to date fruit ripening. Materials and Methods: The study collected four varieties of date fruits from a farm in Riyadh, Saudi Arabia. In it, 10-14-day intervals separated the various stages of maturity. The process of sample preparation and extraction includes the samples of fruits at different stages of maturation. Results and Discussion: The Polyphenols oxidize activity appeared to decline with further maturation of date fruit. There was an increase in the protein content and the reducing sugar in all varieties of dates. The changes in the total sugar and reducing sugar occurred due to the increased enzyme’s activity. Conclusion: The reducing sugar increased in all varieties of the date fruit. The coordination of the enzyme might influence the quality of the ripening of date fruits.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89889135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use","authors":"D. Y. Rahman, S. Praharyawan, S. Raharjo, F. Fadiyah, D. Susilaningsih","doi":"10.14203/ANN.BOGOR.2019.V23.N1.13-19","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2019.V23.N1.13-19","url":null,"abstract":"Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85783210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of Salt Tolerance in Soybean (Glycine Max. L) by Plant-Growth Promoting Endophytic Bacteria Produce 1-Aminocyclopropane-1-Carboxylase Deaminase","authors":"R. Simarmata, N. Ngadiman, S. Rohman, P. Simanjuntak","doi":"10.14203/ANN.BOGOR.2018.V22.N2.81-93","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.81-93","url":null,"abstract":"     Salinity is a major abiotic stress that can induce ethylene synthesis beyond the normal limits as plants response to stress and hence reduces crop productivity. The 1-aminocyclopropane-1-carboxylase deaminase (ACCD)-producing bacteria can reduce excessive ethylene synthesis by taking ACC (ethylene precursor) as a nitrogen source. This study showed the possibility of using endophytic bacteria in order to reduce the undesirable effects of salinity. Strain Pseudomonas putida PIR3C and Roultella terrigena PCM8 exhibited promising performance for promoting the growth of plant under salinity stress conditions. The results showed that bacterial inoculation was effective even in the presence of higher salinity levels. Strain P. putida PIR3C was the most efficient strain compared to the other strains and significantly increased shoot length, root length, dry weight, germination percentage, and reduced stem diameter. The role of ACCD in reducing ethylene production under salinity stress conditions was also studied by measuring the evolution of ethylene in vitro by soybean seeds treated with some ACCD bacterial strain. The maximum ethylene lowering capacity was observed in R. terrigena PCM8, the strain reduced ethylene production from 622.81 nmol.g-1(control) to 352.78 nmol.g-1 (43% reduction). The production of α-ketobutyrate, chlorophyll content and germination percentage from P. putida PIR3C was higher than other strains. The results suggested that strain P. putida PIR3C and R. terrigena PCM8 can be employed for salinity tolerance in soybean seedlings and may have better prospects for an amelioration of stress condition.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84962892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Expression Condition, Two Dimensional And Melting Point-Based Characterization of Recombinant Human Interferon Alpha-2a Fusion and Non Fusion Forms","authors":"R. A. Ningrum, Widdya Kusuma Wardhani, I. Wahyuni, A. Z. Mustopa","doi":"10.14203/ANN.BOGOR.2018.V22.N2.57-64","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.57-64","url":null,"abstract":"     Recombinant Human Interferon Alpha-2a (rhIFNα-2a) is a therapeutic protein that used in hepatitis and cancer treatments. In our previous research, we developed higher molecular weight of the protein through human serum albumin fusion. The fusion and non fusion form of rhIFNα-2a were produced in Pichia pastoriswith 86 kDa and 19 kDa in size respectively. In previous research, protein yield was not reproducible due to unoptimized expression conditions. This reseach was aimed to optimize expression condition process and to characterize the fusion and non fusion forms of rhIFNα-2a. The parameters to observe in overproduction include nutrient (media and methanol concentration) and non nutrient (temperature andincubation period). Affinity and size exclusion cromatographicwere compared in protein purification. BCA assay was used to determine quantity of protein. Protein characterization was conducted using two-dimensional SDS PAGE and denaturation analyses. The optimal condition of expression was achieved using complex media with 1% of methanol for 3 day incubation period at 25°C. The protein yield was reproducible and higher comparing to previous research. Affinity chromatography resulted in higher purity of the proteins comparing to size exclusions. Characterization using two dimensional gel analysis revealed that isoelectric point of rhIFNα-2a is 6.5 for fusion form and 6.0 for non fusion form. The melting points of fusion protein were 56°C and 62°C whilst that of non fusion was 56°C.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88358154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non Invasive Detection of Dengue Viruses from Saliva: In vitro Study","authors":"Budi Saksono","doi":"10.14203/ANN.BOGOR.2018.V22.N2.75-80","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.75-80","url":null,"abstract":"      In the previous paper, we had succeeded in developing an early detection system of dengue viruses using Sugar liganded Gold Nano Particle (SGNP) only from 6 μL serum. It has been reported that dengue virus is also detected in the saliva and urine of the patient. The evidences lead to the possibility of developing non-invasive methods of dengue virus detection. In this in vitro study, we evaluated the utility of SGNP to capture and concentrate dengue virion in 10% saliva solution. The results showed that dengue virion was successfully detected in 10% of saliva solution. Analysis of virion stability during storage showed that virions in salivary samples were stable up to 3 days at temperature wherease the RNA has significantly degraded. Although still a preliminary study, the data obtained show the prospect of SGNP as a non-invasive dengue virus detection method, as well as the development of POC (Point of Care) method. Clinical trials using saliva from dengue viruses infected patients need to be done to prove the effectiveness of the SGNP method.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89036545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Docking and Molecular Dynamics Study of Alcoholic Compounds as Mycobactericidal Agents Using InhA, MabA and PanK as Receptors","authors":"G. Syahputra, A. Arwansyah, W. Kusharyoto","doi":"10.14203/ANN.BOGOR.2018.V22.N2.101-115","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.101-115","url":null,"abstract":"     Tuberculosis (TB) infection is one of the primaryinfectious diseases in many developing countries; even there are minor cases in some developed countries. TB infection spread through the air and ismore probable when using improper disinfectant on medical and laboratory equipment which related to TB research. The appropriate disinfectants which are commonly usedin laboratory equipment can reduce the risk of transmission of TB disease. Alcoholic compoundsare one of the common disinfectants with a broad spectrum activity towardsmicrobes,viruses, and fungus. We employed molecular docking and molecular dynamics simulation to support virtual screening and ligand-receptor complex binding observation in searching for an appropriate mycobactericidal agent.Based on the analysis of molecular docking and molecular dynamics, pentadecanol has potency as a mycobactericidal agent with PanK as itsspecific receptor. The Gibbs free energy (∆G) for the interaction of pentadecanol with PanKhas been found to be -5.5 kcal/mol. Molecular dynamics analysis at 300K and 1 atm for 5 ns showed a little change in the confirmation of the binding site, whilepentadecanolwas still being bound by its binding siteon PanK.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83478108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}