International Research Journal of Insect Sciences最新文献

Diagnostic expression of the cotton pink bollworm Pectinophora gossypiella (Saunders) Ace1 exposed to some insecticide by the real-time PCR 实时荧光定量PCR检测杀虫剂对棉粉棉铃虫Ace1的诊断性表达

International Research Journal of Insect Sciences Pub Date : 2023-05-30 DOI: 10.18488/irjis.v8i1.3387

H. Diab

{"title":"Diagnostic expression of the cotton pink bollworm Pectinophora gossypiella (Saunders) Ace1 exposed to some insecticide by the real-time PCR","authors":"H. Diab","doi":"10.18488/irjis.v8i1.3387","DOIUrl":"https://doi.org/10.18488/irjis.v8i1.3387","url":null,"abstract":"The Pink Bollworm Pectinophora gossypiella (Saunders) is a devastating pest in Egyptian cotton field. Insecticide control failure may be happen due to its biological features of hide inside bolls and rarely exposed to insecticide application. Thus, a bulk of bolls infested with larvae collected from fields of Sharkia and Benysuef governorate. Moreover, exposed to py, Op sub lethal concentrations in laboratory bioassay. Toxicity features as LC50, LC90 and the slope, and RR was completed. Whereas the common target site of OP and pyrethroid is the acetylcholinesterase enzyme. Thus, the gene Ace1 encoding AChE relative expression was investigated using molecular marker of real-time PCR procedures via the steps of generating data was RNA isolation and characterization, cDNA synthesis, then generating normalization factors, and Ct data attained and analyzed using 2-ΔΔCT method to quantify the gene expression quantitatively of insecticide treated larvae tissue extracted. Data showed the most expressed ratio of samples was cypermethrin followed by deltamethrin compared with the control and the least was malathion and chlorpyrifos, thus, the most effective insecticide was malathion and the least were cypermethrin according to ct values. Hierarchical clustering combined with Heat map and principle component analysis based on various clustering systems and algorithm as distance measures were completed. Validation of reference gene in addition to gene expression stability analyzed by the three optimal gene finders and showed that deltamethrin and cypermethrin treated genes having lower mean weights and considered transcriptionally stable and ideal reference genes.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122165560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional characterization of some major edible insects around Wukari, Taraba state, Nigeria 尼日利亚塔拉巴州乌卡里地区一些主要食用昆虫的营养特征

International Research Journal of Insect Sciences Pub Date : 2023-02-13 DOI: 10.18488/irjis.v8i1.3286

Okrikata Emmanuel, O. J. Itohan, Ogunmola Adeniyi Oyewole

{"title":"Nutritional characterization of some major edible insects around Wukari, Taraba state, Nigeria","authors":"Okrikata Emmanuel, O. J. Itohan, Ogunmola Adeniyi Oyewole","doi":"10.18488/irjis.v8i1.3286","DOIUrl":"https://doi.org/10.18488/irjis.v8i1.3286","url":null,"abstract":"Many people go for edible insects in sub-saharan Africa in order combat hunger and to satisfy appetite. Though, generally known as good source of proteins; if insects contain antinutrients in disproportionate levels, they can pose health risk to humans. This study therefore analysed the nutritional and antinutritional components of some insects commonly consumed around Wukari, Nigeria: Shea caterpillar – Cirina butyrospermi Vuillet (Lepidoptera: Saturniidae), Termite – Macrotermes bellicosus Smeathman (Isoptera: Termitidae), Cricket – Gryllus bimaculatus De Geer (Orthoptera: Gryllidae) and Grasshopper - Zonocerus variegatus (Orthoptera: Pyrgomorphidae) vis-à-vis African catfish – Clarias gariepinus Burchel (Siluriformes: Clariidae) which served as control. Proximate, mineral and the antinutrient component were analysed using standard procedures. Results revealed that grasshoppers had more moisture, fat and carbohydrate content than catfish. Also, cricket had an appreciable amount of protein, ash, and fibre content. However, the highest protein content was found in cricket, while grasshoppers contained the least protein. The mineral content analysis revealed that cricket had the highest calcium, magnesium, potassium, sodium and zinc content which differed significantly from catfish. The antinutrient analysis revealed that the least amount of tannins was in grasshoppers and phytates, in crickets. The amounts of antinutrients found in the insect samples are largely comparable to that in catfish. The insects assayed are healthy for human consumption and could serve as nutritional supplements. These findings revealed that the assayed insects contain high nutritional components which can substitute catfish in diets in the communities around the study area, and perhaps, beyond.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127397406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insect predator of spodoptera frugiperda J. E. Smith in Sleman and Gunungkidul regency, Indonesia 印度尼西亚Sleman和Gunungkidul县狐翅目昆虫捕食者

International Research Journal of Insect Sciences Pub Date : 2023-02-03 DOI: 10.18488/irjis.v8i1.3278

I. Putra, Novia Saphira Prakusya, Defi Yuliasari

{"title":"Insect predator of spodoptera frugiperda J. E. Smith in Sleman and Gunungkidul regency, Indonesia","authors":"I. Putra, Novia Saphira Prakusya, Defi Yuliasari","doi":"10.18488/irjis.v8i1.3278","DOIUrl":"https://doi.org/10.18488/irjis.v8i1.3278","url":null,"abstract":"This research was conducted to identify and found the abundance of predatory insect species of S. frugiperda in Sleman and Gunungkidul Regencies, Yogyakarta Province, Indonesia. This research was conducted in June 2021 - March 2022 on corn plantations in Sleman and Gunungkidul Regencies. Determination of the sampling location was determined by stratified random sampling. Corn land used in sampling was determined by purposive. Insect predator caught by using sweeping net and hand picking. The results showed that there are 3 orders, 8 families, and 16 species of insect predators. 3 orders of the insect predators that found in the Sleman and Gunungkidul Regencies were Coleoptera, Hymenoptera, and Hemiptera. Insect predator families that found in Sleman dan Guungkidul Regencies were Coccinellidae, Carabidae, Staphylinidae, Formicidae, Vespidae, Pentatomidae and Reduviidae. Insect predators species that was most commonly found was Menochilus sexmaculatus with 467 individuals and the least was Sycanus sp. with 1 individual. The conclusion of this study is that there were 16 species of predators from S. frugiperda in Sleman and Gunungkidul Regencies. Practical implications of this research were the insect predators that found in Sleman and Gunungkidul Regencies can be used to maintain the population of Spodoptera frugiperda in two regencies.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123664031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodemographic parameters of spodoptera frugiperda smith, 1797 (Lepidoptera: Noctuidae), a pest of maize crop, zea mays linnaeus, 1753 in sub-sudanese zone of Côte d'Ivoire 科特迪瓦Côte苏丹以南地区玉米作物害虫玉米螟(zea mays linnaeus, 1753)， 1797(鳞翅目:夜蛾科)生物统计学参数

International Research Journal of Insect Sciences Pub Date : 2023-02-01 DOI: 10.18488/irjis.v8i1.3272

Stephanie Manuela Klamansoni Akissi Konan, Laya Kansaye, Henoc Jacques Meva Dakouri, Nondenot Roi Louis Aboua

{"title":"Biodemographic parameters of spodoptera frugiperda smith, 1797 (Lepidoptera: Noctuidae), a pest of maize crop, zea mays linnaeus, 1753 in sub-sudanese zone of Côte d'Ivoire","authors":"Stephanie Manuela Klamansoni Akissi Konan, Laya Kansaye, Henoc Jacques Meva Dakouri, Nondenot Roi Louis Aboua","doi":"10.18488/irjis.v8i1.3272","DOIUrl":"https://doi.org/10.18488/irjis.v8i1.3272","url":null,"abstract":"Spodoptera frugiperda is one of the main pests of maize (Zea mays L.) in Côte d'Ivoire. The control of this insect requires knowledge of biological and demographic parameters. The need to know the effect of seasons on the biodemographic parameters of Spodoptera frugiperda in Côte d'Ivoire led us to carry out work in the Sub-sudanese zone. The study was carried out in dry and rainy seasons on a maize plot from 2020 to 2021. The monitoring of newly emerged individuals on 3-week-old early variety with semi-serrated grains (EV 87/28-SR) maize plants under semi-natural conditions. The development cycle consisted of six larva stages. The development time was 27.93 ± 1.01 days in dry season and 32.47 ± 1.83 days in rainy season. Larval survival rates ranged from 85.79 to 100% in dry season and from 74.64 to 94.7% in rainy season. The average egg fertility rate was 21.19 ± 5.89% in dry season and 40.36 ± 8.49% in rainy season. The sex ratio was in favour of females. The net reproduction rate (Ro) and intrinsic rate of natural increase (rm) were highest in rainy season at 27.67 ± 7.82 and 0.26 ± 0.01 respectively. Spodoptera frugiperda performs much better in rainy season at temperatures between 25.1°C - 26.2°C due to a slow development time of 32.47 ± 1.83 days, a short population doubling time (D.T.) of 2.62 ± 0.13 and a high net reproduction rate (Ro) of 27.67 ± 7.82.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134103038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inventory of Termites and Biogenic Structures in the Forest Adjoining the "Palm Trees" Restaurant of Felix Houphouet-Boigny University Campus (Abidjan - South Cote D'ivoire) 费利克斯·乌弗埃-博瓦尼大学校园“棕榈树”餐厅附近森林白蚁和生物结构的调查(阿比让-科特迪瓦南部)

International Research Journal of Insect Sciences Pub Date : 2022-09-29 DOI: 10.18488/irjis.v7i1.3142

Akpesse Akpa Alexandre Moise, Bassa Amenan N’Nezou Victoire, Diabaté Dohouonan, Coulibaly Tenon, Kissi Thérèse Appoh Perrine, K. Hervé, K. Philippe

{"title":"Inventory of Termites and Biogenic Structures in the Forest Adjoining the \"Palm Trees\" Restaurant of Felix Houphouet-Boigny University Campus (Abidjan - South Cote D'ivoire)","authors":"Akpesse Akpa Alexandre Moise, Bassa Amenan N’Nezou Victoire, Diabaté Dohouonan, Coulibaly Tenon, Kissi Thérèse Appoh Perrine, K. Hervé, K. Philippe","doi":"10.18488/irjis.v7i1.3142","DOIUrl":"https://doi.org/10.18488/irjis.v7i1.3142","url":null,"abstract":"Termites play an important ecological role. Thus, this study was carried out in the forest adjoining restaurant \"the palm trees of the university campus of Cocody\" to determine the species of termites and their impacts on the trees of this forest. Three transects of 100m long and 2m wide each subdivided into 20 sections and Tropical soil biology and fertility (TSBF) monoliths were developed. Then a systematic excavation was carried out along transects in search of termites. Then the plot containing transects was prospected in order to identify termite mounds and biogenic structures. After inventory, we identified 7 species of termites: Macrotermes bellicosus, Microtermes sp, Basidentitermes sp, Odontotermes sp, Pericapritermes sp, Amitermes guineensis and Ancistrotermes guineensis. Wood-feeders, fungus-growers and soil-feeders are the trophic groups found on the site. Fungus-growers were the group with the most species. We recorded a 25% attack rate on the trees from transects. A total of 13 nests were counted including 9 epigeal termite mounds and 4 arboreal nests. The low richness recorded shows that our plot is heavily anthropized. The conservation or maintenance of this site would allow species to recolonize this space.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"18 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114028013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cassava-Legume Intercropping Systems on the Population Dynamics of Legumes Insect Pests in Three Major Agro-Climatic Zones of Sierra Leone 木薯-豆科作物间作制度对塞拉利昂三大农业气候带豆科害虫种群动态的影响

International Research Journal of Insect Sciences Pub Date : 2022-08-05 DOI: 10.18488/irjis.v7i1.3086

A. Mansaray, A. B. Karim, T. Yormah, A. Conteh

引用次数: 0

Diversity, Geographical Habitat Usage and Distribution Assessment of Flies (Insecta: Diptera) in Nilgiri Hills, Tamil Nadu, India 印度泰米尔纳德邦Nilgiri山蝇类多样性、地理生境利用及分布评价(昆虫科:双翅目

International Research Journal of Insect Sciences Pub Date : 2022-05-06 DOI: 10.18488/irjis.v7i1.2982

N. Moinudheen, A. Samson

引用次数: 0

DNA Barcode for the Parthenogenetic Surinam Cockroach Pycnoscelus surinamensis (Linnaeus, 1758) (Blattodea: Blaberidae) from India 印度单性生殖苏里南蜚蠊(Linnaeus, 1758)(小蠊总目:小蠊科)的DNA条形码

International Research Journal of Insect Sciences Pub Date : 2022-01-13 DOI: 10.18488/irjis.v7i1.2895

A. Shabnam, K. Dinesh

引用次数: 0

Oviposition Deterrence and Ovicidal Activity of Birbira (Melletia furrgunia) on Diamondback Moth (Plutella xylostella) (Lepidoptera: Plutellidae) 灰Birbira (Melletia furgunia)对小菜蛾(小菜蛾)的阻卵及杀卵活性(鳞翅目:小菜蛾科)

International Research Journal of Insect Sciences Pub Date : 2021-12-28 DOI: 10.18488/journal.106.2021.61.8.14

A. Nega, B. Mulatu

引用次数: 0

Death in the Mouth: The Current Status of Pesticide Residues in Cured Fish from Selected Markets in Rivers State Nigeria 口中死亡:尼日利亚河流州选定市场腌鱼中农药残留的现状

International Research Journal of Insect Sciences Pub Date : 2020-04-27 DOI: 10.18488/journal.106.2020.51.14.22

Bob-Manuel R.B, Egbecho E, Z. U

{"title":"Death in the Mouth: The Current Status of Pesticide Residues in Cured Fish from Selected Markets in Rivers State Nigeria","authors":"Bob-Manuel R.B, Egbecho E, Z. U","doi":"10.18488/journal.106.2020.51.14.22","DOIUrl":"https://doi.org/10.18488/journal.106.2020.51.14.22","url":null,"abstract":"Pesticide residues in cured fish from selected markets in Rivers State, Nigeria were determined to compare their residues content with FAO/WHO MRLs. The research was carried out at Ignatius Ajuru University of Education and Austino Research Laboratories, Rivers State, Nigeria. Samples of cured fish were collected from different major markets in Rivers State, Nigeria. Each product was purchased from three different traders and kept in an air tight Ziploc envelope containing general information. Some of the samples were kept in 250 g plastic containers and kept on a laboratory bench for a period of 45 days to observe insect emergence while the other lots were taken to Austino Research Laboratory for pesticide residue analysis. The result shows varying degrees of damage ranging from slightly to undamaged and mean number of D. maculatus emergence was higher in smoked fish samples obtained at Rumuokwuta. The result also shows that in all the cured fish sampled, DDT, Captan, Hexazinone, and Tecnazene were found above the FAO/WHO MRLs and few compounds were below the MRLs. Conclusively, relevant authorities and agencies needs to increase surveillance and monitoring to check and control the illegal importation, sale and use of such compounds especially in controlling stored pests of cured fish. This could reduce cases of pesticide toxicity and food poisoning and other health implications due to its ingestion in such contaminated cured fish. content with FAO/WHO MRLs.","PeriodicalId":403262,"journal":{"name":"International Research Journal of Insect Sciences","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131616555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0