Novel Forensic Research最新文献

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Psychotherapy of post-traumatic stress disorder in the context of process and outcome studies 创伤后应激障碍心理治疗的过程与结果研究

Novel Forensic Research Pub Date : 1900-01-01 DOI: 10.5455/nofor.2022.08.04

Bilge Celebi

引用次数: 0

Mitochondrial DNA (8389-8865 base pairs) mutation search in Turkish population 土耳其人群线粒体DNA(8389-8865碱基对)突变研究

Novel Forensic Research Pub Date : 1900-01-01 DOI: 10.5455/nofor.2023.03.06

O. Karatas, H. Altuncul, Nazli Holumen, I. Erkan, D. Islek, E. Yukseloglu

{"title":"Mitochondrial DNA (8389-8865 base pairs) mutation search in Turkish population","authors":"O. Karatas, H. Altuncul, Nazli Holumen, I. Erkan, D. Islek, E. Yukseloglu","doi":"10.5455/nofor.2023.03.06","DOIUrl":"https://doi.org/10.5455/nofor.2023.03.06","url":null,"abstract":"Aim: STR loci in nuclear DNA (nDNA) is generally used for identification and identity determination in forensic sciences. However, it is not always possible to achieve successful results from these loci. Especially in biological samples containing nDNA that are degraded in quantity and quality, there might be difficulties to analyze. In such cases, it may be more reliable to use mitochondrial DNA (mtDNA) analysis. Because there is only one nDNA in the cell, while there are many copies of mtDNA. Therefore, it is advantageous to work with. HVI, HVII and HVIII regions in mtDNA are used for identification purposes in forensic sciences. However, the discrimination power of these regions are limited. As an alternative to these regions, studying the coded region will increase the discrimination power. For this reason, the aim of our study is to distinguish the points showing polymorphism in the region between 8389-8865 base pairs as an alternative to the HVI, HVII and HVIII regions. In addition, it will be optimized for routine use in biological samples that do not contain enough nDNA with the regions where the polymorphism rate is determined. \u0000Materials and Methods: In our study, blood samples were taken from 150 volunteers from the Turkish population who were not related. The region in question was reproduced in accordance with the method of PCR Tzen et al. The obtained sequences were compared with the Cambridge Reference Sequence. \u0000Results: The most common polymorphisms were found to be 8860G, 8697A, 8847delC, 8691delA. The discrimination power of the studied region was calculated to be 0.923641, the probability of random matching was 0.076359 and the genetic diversity was calculated as 0.9303827. \u0000Conclusion: In line with these data, it was determined that we could distinguish between individuals with the region we examined and that we could increase the discrimination power by analyzing this region together with the HVI, HVII, HVIII regions.","PeriodicalId":402171,"journal":{"name":"Novel Forensic Research","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127460280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-Approached technology applications in forensic genetics rna技术在法医遗传学中的应用

Novel Forensic Research Pub Date : 1900-01-01 DOI: 10.5455/nofor.2022.06.01

E. Yukseloglu

{"title":"RNA-Approached technology applications in forensic genetics","authors":"E. Yukseloglu","doi":"10.5455/nofor.2022.06.01","DOIUrl":"https://doi.org/10.5455/nofor.2022.06.01","url":null,"abstract":"Ribonucleic acid (RNA) is a nucleic acid which is structurally different from DNA. DNA is the most used and approved nucleic acid in routine applications in forensic caseworks. In this article, giving an information about which RNA types are chosen for which forensic applications is aimed. RNA technologies are developing in the concept of forensic genetics and they can be adapted into routine case works in the case of well conditions are supplied. Both coding and non-coding RNAs are investigated for forensic purposes and most examined ones are messenger RNAs, and microRNAs. There are some researches on circular RNAs and piwi-interacting RNAs but they are in low number when compared with first two RNA types. Forensic studies based on RNA technologies are body fluid identification, post mortem interval determination, determination of stain age, estimation of an individual’s age and sex, identification of organ tissues, wound age estimation, and determination of drug abuse. Lastly, different RNA based technologies can be used in these studies and some of them are micro-array, Nano-String technology, real time PCR, end point PCR, high resolution melt (HRM) analysis and next generation sequencing (NGS) technology.","PeriodicalId":402171,"journal":{"name":"Novel Forensic Research","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128625868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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