Current Topics in Peptide and Protein Research最新文献

Estimation of hydrophobicity of segments in Amyloid β42partly comprising D-amino acids 淀粉样蛋白β42部分含d -氨基酸片段的疏水性估计

Current Topics in Peptide and Protein Research Pub Date : 2021-12-31 DOI: 10.31300/ctppr.22.2021.17-45

T. Munegumi

引用次数: 0

Activity of breast milk antimicrobial peptides in experiments in vitro 母乳抗菌肽活性的体外实验研究

Current Topics in Peptide and Protein Research Pub Date : 2021-12-30 DOI: 10.31300/ctppr.22.2021.77-83

T. Kolyganova, V. Arzumanian, E. Budanova, Oxana A. Svitich, N. Vartanova, V. Zverev

引用次数: 0

Antimicrobial activities of human biofluids and their antimicrobial peptide fractions against Candida albicans 人体液及其抗菌肽组分对白色念珠菌的抑菌活性

Current Topics in Peptide and Protein Research Pub Date : 2019-09-04 DOI: 10.31300/ctppr.20.2019.35-40

V. Arzumanian, T. Erofeeva, P. Zhigalkina, O. Svitich

{"title":"Antimicrobial activities of human biofluids and their antimicrobial peptide fractions against Candida albicans","authors":"V. Arzumanian, T. Erofeeva, P. Zhigalkina, O. Svitich","doi":"10.31300/ctppr.20.2019.35-40","DOIUrl":"https://doi.org/10.31300/ctppr.20.2019.35-40","url":null,"abstract":"It is known that human biological fluids contain antimicrobial protective factors that include antimicrobial peptides (AMP). Concentrations of AMP vary in different biofluids and tissues. The aim of the present study is to estimate the total antimicrobial activity of different human biofluids in comparison with the activity of their AMP fractions as markers of local immunity. The antimicrobial activities of serum (SE), vaginal fluid (VF), saliva (SA), water-soluble part of skin secretion (SS) and urine (UR) of 6 healthy women, 22-25 years old, were measured by the spectrophotometric method based on the ability of the above-mentioned biofluids to disrupt the cytoplasmic membrane of Candida albicans [Patent RF No 2602298]. The results were evaluated as the percent of dye penetrated into dead cells of treated yeast cell suspensions compared to the control ones. The medians of total and AMP activities are SE – 86.6% and 33.7%; VF – 24.8% and 21.9%; SA – 18.3% and 10.9%; SS – 12.8% and 2.8%; UR – 10.0% and 0.0%, respectively. The highest level of immune defense occurs in SA whereas the lowest in UR. The antimicrobial activity of AMP in VF is maximal compared to the other biofluids. Levels of total and AMP activities of SA, SS and UR have high positive correlation between each other (Pirson’s correlation coefficients r ≥ 0,700), while none of them correlated with the activities of VF (r ≤ 0,300). Using the most appropriate method of antimicrobial activity estimation the differences between biofluids of healthy human tissues of different localization are demonstrated for the first time.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44235001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the fundamentals of proteomics 了解蛋白质组学的基本原理

Current Topics in Peptide and Protein Research Pub Date : 2019-09-04 DOI: 10.31300/ctppr.20.2019.25-33

S. Alsagaby

引用次数: 5

Activity of antimicrobial peptide fractions of human serum and saliva against clinically important yeasts 人血清和唾液抗菌肽组分对临床重要酵母菌的活性研究

Current Topics in Peptide and Protein Research Pub Date : 2019-09-04 DOI: 10.31300/ctppr.20.2019.9-15

V. Arzumanian, T. Erofeeva, P. Zhigalkina, Asiya Ixanova, O. Svitich

{"title":"Activity of antimicrobial peptide fractions of human serum and saliva against clinically important yeasts","authors":"V. Arzumanian, T. Erofeeva, P. Zhigalkina, Asiya Ixanova, O. Svitich","doi":"10.31300/ctppr.20.2019.9-15","DOIUrl":"https://doi.org/10.31300/ctppr.20.2019.9-15","url":null,"abstract":"Every human bioliquid contains substances possessing direct antimicrobial activity. These substances include complement system proteins of serum, immunoglobulins of serum and epithelial secretions, and antimicrobial peptides/proteins (AMP) with low molecular masses, which are present at all sites and liquids of human body. The aim of this study is to estimate the AMP fraction activity of serum and saliva towards different clinically important yeasts. Specimens of serum and saliva were obtained from 10 healthy volunteers 5 women and 5 men, 20-26 years old. Strains of yeasts Candida albicans, Rhodotorula mucilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cutaneum and Saccharomyces cerevisiae were used in the experiments. Native (total) liquids and their AMP fractions obtained by filtration through a membrane of 100 kDa were used in the process of antimicrobial activity estimation carried out by the spectrophotometric method. The AMP activities were 2.3-13.5 fold lower than the corresponding total ones, the yeasts most susceptible to serum AMP were C. albicans and Cr. neoformans. Total salivary antifungal activities were 3.6-5.2 fold lower than the corresponding total serum activities. Total activity of saliva was significantly higher towards R. mucilaginosa, while that of the other ones were almost the same. AMP activities of saliva were 1.1-3.8 fold lower than the total ones. R. mucilaginosa and T. cutaneum were the most sensitive to AMP fraction of saliva. Gender difference with regard to the activity of the studied bioliquids was also observed: the most significant ratio was obtained for the sensitivity of C. albicans to women/men salivary AMP. Based on the above data one can conclude that among the studied yeast genera/species show specificity to AMP. It is expressed in terms of the varying sensitivity of the yeasts to the AMP fraction in serum and saliva.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42025973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Apolipoprotein E Mimetic Peptide AEM-2 Attenuates Mitochondrial Injury And Apoptosis In Human THP-1 Macrophages. 载脂蛋白E模拟肽AEM-2减轻人THP-1巨噬细胞线粒体损伤和凋亡

Current Topics in Peptide and Protein Research Pub Date : 2018-01-01

Samantha Giordano-Mooga, Geeta Datta, Paul Wolkowicz, David W Garber, Mayakonda Palgunachari, C Roger White, G M Anantharamaiah

{"title":"The Apolipoprotein E Mimetic Peptide AEM-2 Attenuates Mitochondrial Injury And Apoptosis In Human THP-1 Macrophages.","authors":"Samantha Giordano-Mooga, Geeta Datta, Paul Wolkowicz, David W Garber, Mayakonda Palgunachari, C Roger White, G M Anantharamaiah","doi":"","DOIUrl":"","url":null,"abstract":"Cardiovascular disease, specifically atherosclerosis, is exacerbated by hypercholesterolemia. Current therapies that target lipid lowering, however, are not effective in all patients. Apolipoprotein E (apoE) plays an important role in mediating the clearance of plasma cholesterol and also exerts numerous cytoprotective responses. Our laboratory has synthesized novel therapeutics that mimic the ability of apoE to decrease plasma cholesterol. The apoE mimetic peptide AEM-2 is a dual domain peptide composed of an amphipathic helical region that binds phospholipids and a positively charged region that mediates the hepatic clearance of lipoproteins. Administration of AEM-2 to apoE null mice reduced plasma cholesterol concentration by 80% one hour post-administration. Since apoE is also known to exert anti-inflammatory effects that are independent of its ability to lower cholesterol, we tested effects of AEM-2 on lipopolysaccharide-induced responses in human THP-1 macrophages. Pre-treatment of THP-1 cells with AEM-2 significantly reduced the LPS-induced secretion of IL-6 and TNFα. Since LPS administration is associated with an increase in mitochondrial injury, we monitored effects of AEM-2 on mitochondrial function. AEM-2 significantly reduced mitochondrial superoxide formation, prevented the LPS-induced decrease in mitochondrial membrane potential and attenuated the release of cytochrome c. AEM-2 also inhibited the activities of initiator caspases 8 and 9 and effector caspase 3. The attenuation of apoptosis in AEM-2 treated cells was associated with an increase in cellular autophagy. These data suggest that AEM-2 attenuates cellular injury in LPS-treated THP-1 macrophages and facilitates the removal of cellular debris and damaged organelles via induction of autophagy.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019287/pdf/nihms972318.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36268835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

a-Factor: a chemical biology tool for the study of protein prenylation. a-因子:用于研究蛋白质烯酰化的化学生物学工具。

Current Topics in Peptide and Protein Research Pub Date : 2017-01-01

Veronica Diaz-Rodriguez, Mark D Distefano

{"title":"a-Factor: a chemical biology tool for the study of protein prenylation.","authors":"Veronica Diaz-Rodriguez, Mark D Distefano","doi":"","DOIUrl":"","url":null,"abstract":"The mating pheromone a-factor is a lipidated dodecapeptide found in the budding yeast S. cerevisiae. The biosynthesis of this peptide encompasses the same three-step processing pathway (farnesylation of C-terminal cysteine, C-terminal proteolysis and C-terminal methyl esterification) as Ras proteins, mutated forms of which have been found in ~30% of human cancers. For the mating of two haploid yeast cells into a diploid cell to happen, the a-factor pheromone travels to the cell surface of the opposite mating cell, where it binds and activates a G-protein coupled receptor. This lipidated-peptide/protein interaction has caught the attention of researchers studying protein prenylation, and studies have shown that this peptide can be used as a model system to understand the role of prenyl groups in protein-protein interactions. Here, we review the different methods used for the synthesis of a-factor and a-factor analogs containing C-terminal cysteine esters and the assays developed for detecting pheromone bioactivity and quantitation of pheromone efficiency. Also, we review crucial peptide modifications that have been used to investigate relationships between the structure and activity of this lipopeptide with its receptor Ste3p. Finally, we aim to discuss recent and future applications of a-factor as a chemical biology tool to study protein prenylation. These include the use of photo crosslinking reactions to map peptide/receptor interactions, the addition of fluorophore tags to visualize the peptide binding, and the use of bio-orthogonal reactions for protein labeling and protein purification.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915288/pdf/nihms959833.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36053939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating the dysregulated migration of pulmonary arterial hypertensive smooth muscle cells with motif mimicking cell permeable peptides. 用模拟细胞通透性肽图案调节肺动脉高压平滑肌细胞的迁移失调。

Current Topics in Peptide and Protein Research Pub Date : 2015-01-01

Jamie L Wilson, Chamila Rupasinghe, Anny Usheva, Rod Warburton, Chloe Kaplan, Linda Taylor, Nicholas Hill, Dale F Mierke, Peter Polgar

{"title":"Modulating the dysregulated migration of pulmonary arterial hypertensive smooth muscle cells with motif mimicking cell permeable peptides.","authors":"Jamie L Wilson, Chamila Rupasinghe, Anny Usheva, Rod Warburton, Chloe Kaplan, Linda Taylor, Nicholas Hill, Dale F Mierke, Peter Polgar","doi":"","DOIUrl":"","url":null,"abstract":"Migration of vascular smooth muscle cells is a key element in remodeling during pulmonary arterial hypertension (PAH). We are observing key alterations in the migratory characteristics of human pulmonary artery smooth muscle cells (HPASMC) isolated from transplanted lungs of subjects with PAH. Using wound migration and barrier removal assays, we demonstrate that the PAH cells migrate under quiescent growth conditions and in the absence of pro-migratory factors such as platelet derived growth factor (PDGF). Under the same conditions, in the absence of PDGF, non-PAH HPASMC show negligible migration. The dysregulated migration initiates, in part, through phosphorylation events signaled through the unstimulated PDGF receptor via focal adhesion kinase (FAK) whose total basal expression and phosphorylation at tyrosine 391 is markedly increased in the PAH cells and is inhibited by a motif mimicking cell-permeable peptide (MMCPP) targeting the Tyr751 region of the PDGF receptor and by imatinib. However, exposure of the PAH cells to PDGF further promotes migration. Inhibition of p21 activated kinases (PAK), LIM kinases (LIMK), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) reduces both the dysregulated and the PDGF-stimulated migration. Immunofluorescence microscopy confirms these observations showing activated JNK and p38 MAPK at the edge of the wound but not in the rest of the culture in the PAH cells. The upstream inhibitors FAK (PF-573228) and imatinib block this activation of JNK and p38 at the edge of the site of injury and correspondingly inhibit migration. MMCPP which inhibit the activation of downstream effectors of migration, cofilin and caldesmon, also limit the dysregulated migration. These results highlight key pathways which point to potential targets for future therapies of pulmonary hypertension with MMCPP.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888800/pdf/nihms-761889.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34459962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptic activity within the Type III₁ domain of fibronectin regulates tissue inflammation and angiogenesis. 纤维粘连蛋白 III1 型结构域内的隐性活性可调节组织炎症和血管生成。

Current Topics in Peptide and Protein Research Pub Date : 2015-01-01

Christina Cho, Rhiannon Kelsh-Lasher, Anthony Ambesi, Paula J McKeown-Longo

{"title":"Cryptic activity within the Type III1 domain of fibronectin regulates tissue inflammation and angiogenesis.","authors":"Christina Cho, Rhiannon Kelsh-Lasher, Anthony Ambesi, Paula J McKeown-Longo","doi":"","DOIUrl":"","url":null,"abstract":"The fibronectin matrix provides mechanical and biochemical information to regulate homeostatic and pathological processes within tissues. Fibronectin consists of independently-folded modules termed Types I, II and III. In response to cellular contractile force, Type III domains unfold to initiate a series of homophilic binding events which result in the assembly of a complex network of intertwining fibrils. The unfolding of Type III modules provides elasticity to the assembled fibronectin matrix allowing it to function as a dynamic scaffold which provides binding sites for cellular receptors, growth factors and other matrix molecules. Access to bioactive sites within the fibronectin matrix is under complex regulation and controlled through a combination of mechanical and proteolytic activity. Mechanical unfolding of Type III modules and limited proteolysis can alter the topographical display of bioactive sites within the fibronectin fibrils by exposing previously cryptic sites and disrupting functional sites. In this review we will discuss cryptic activity found within the first Type III module of fibronectin and its impact on tissue angiogenesis and inflammation.","PeriodicalId":39897,"journal":{"name":"Current Topics in Peptide and Protein Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981920/pdf/nihms-806346.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34369516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and screening of peptide libraries with free C-termini. 含游离c末端肽库的合成与筛选。

Current Topics in Peptide and Protein Research Pub Date : 2014-01-01

Yen-Chih Wang, Mark D Distefano

引用次数: 0