Tefera Tadesse, B. Kebede, D. Yimer, M. Workie, Tirsit Tibebu, Sewunet Abera, Adaba Tilahun, M. Alemu, Tadessa Daba, Adane Eshetu, Birhanu Babiye, Gudeta Dida
{"title":"Screening and characterization of potential cyanide tolerating lactic acid bacteria from cassava pulp juice","authors":"Tefera Tadesse, B. Kebede, D. Yimer, M. Workie, Tirsit Tibebu, Sewunet Abera, Adaba Tilahun, M. Alemu, Tadessa Daba, Adane Eshetu, Birhanu Babiye, Gudeta Dida","doi":"10.53430/ijbpru.2021.1.1.0025","DOIUrl":"https://doi.org/10.53430/ijbpru.2021.1.1.0025","url":null,"abstract":"Lactic acid bacteria are predominant microorganisms that play important roles during cassava fermentation. Most efforts to develop cyanide tolerant isolates for cassava fermented products have been unsuccessful because of cyanide toxicity in cassava tubers. The objectives of this study were identifying cyanide tolerating bacteria and study their tolerability efficiency. 119 lactic acid bacteria were isolated from fermented cassava pulp juice. Out of the total isolated bacteria, ten potential cyanide tolerating bacteria was screened. The cyanide tolerance efficiency was studied at 100, 400, 600 and 8000mg/L potassium cyanide, growth at different temperature and pH were analyzed. The maximum growth rate reading of the screened isolates ranges (0.674 to 1.116) at 600nm in maximum cyanide concentration. Isolate CD1, LA2, LA1 and CD2 showed maximum cyanide tolerance of (74.4, 72.66, 70.66 and 68.73%) respectively at 800mg/l after 48hr of incubation. The cyanide tolerability potential of individual isolates varies across different cyanide concentration. Above all 10 isolates were resistant to maximum cyanide concentration and possess potential of cyanide tolerating properties that help their selection and application in a controlled process to detoxify cassava roots for food and feed utilization. There was a general decrement in all samples over 48 h fermentation periods from 6.00 to 3.18 and the bacterial isolates showed significant growth at room temperature which were between ʺ25°Cʺ and ʺ40°Cʺ.","PeriodicalId":393912,"journal":{"name":"International Journal of Biology and Pharmacy Research Updates","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130128554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of exclosure on the vegetation composition and structure of Northern Ethiopia","authors":"Samson Shimelse, Hailu Atnafu","doi":"10.53430/ijbpru.2021.1.1.0024","DOIUrl":"https://doi.org/10.53430/ijbpru.2021.1.1.0024","url":null,"abstract":"Land rehabilitation by area exclosure was the fastest and moderate approach of recovering degraded sites. The objective of the study is to investigate the impact of exclosure establishment on the vegetation ecology of the Northern Ethiopia.A stratified preferential sampling design technique with flexible systematic model was used for data collection. Data on vegetation and environmental parameters were gathered from 120 quadrants (90 from restorations or exclosures of different ages and 30 from adjacent free grazing lands), of 20 m x 20 m (400 m2) size. Species richness and the presence or absence of herbaceous plants were recorded like soil samples in a 2 m x 2 m (4 m2) subplot inside each main quadrant from five points, one at each corner and one at the center. A total of 142 plant species belonging to 118 genera and 52 families were identified. All exclosures displayed higher plant species richness, diversity, and aboveground standing biomass compared to the adjacent free grazing lands. Consideration of edaphic (e.g. soil total nitrogen, available phosphorus, CEC, exchangeable bases, soil pH and soil texture) and site (e.g. Stoniness, Grazing) variables will help to optimize the selection of areas for the establishment of future exclosures. Moreover, our study suggests that with time exclosures may increasingly obtain an important role as refugees and species pool similar to church forests and should be protected and managed in a sustainable manner. Substantial numbers of forest species were found to have irregular population structure and are in reduced regeneration status. To prevent local extinction of these species, present efforts of nursery establishment and plantation of indigenous species in the exclosures should be strengthened and extended.","PeriodicalId":393912,"journal":{"name":"International Journal of Biology and Pharmacy Research Updates","volume":"9 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131102068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}