In Silico Biology最新文献_第2页

Dynamical modeling of pro- and anti-inflammatory cytokines in the early stage of septic shock. 感染性休克早期促炎性和抗炎性细胞因子的动力学模型。

In Silico Biology Pub Date : 2020-01-01 DOI: 10.3233/ISB-200474

J Tallon, B Browning, F Couenne, C Bordes, F Venet, P Nony, F Gueyffier, V Moucadel, G Monneret, M Tayakout-Fayolle

{"title":"Dynamical modeling of pro- and anti-inflammatory cytokines in the early stage of septic shock.","authors":"J Tallon, B Browning, F Couenne, C Bordes, F Venet, P Nony, F Gueyffier, V Moucadel, G Monneret, M Tayakout-Fayolle","doi":"10.3233/ISB-200474","DOIUrl":"https://doi.org/10.3233/ISB-200474","url":null,"abstract":"A dynamical model of the pathophysiological behaviors of IL18 and IL10 cytokines with their receptors is tested against data for the case of early sepsis. The proposed approach considers the surroundings (organs and bone marrow) and the different subsystems (cells and cyctokines). The interactions between blood cells, cytokines and the surroundings are described via mass balances. Cytokines are adsorbed onto associated receptors at the cell surface. The adsorption is described by the Langmuir model and gives rise to the production of more cytokines and associated receptors inside the cell. The quantities of pro and anti-inflammatory cytokines present in the body are combined to give global information via an inflammation level function which describes the patient's state. Data for parameter estimation comes from the Sepsis 48 H database. Comparisons between patient data and simulations are presented and are in good agreement. For the IL18/IL10 cytokine pair, 5 key parameters have been found. They are linked to pro-inflammatory IL18 cytokine and show that the early sepsis is driven by components of inflammatory character.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-200474","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38097351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Moonlighting proteins - an approach to systematize the concept. 兼职蛋白质-一种系统化概念的方法。

In Silico Biology Pub Date : 2020-01-01 DOI: 10.3233/ISB-190473

Maria Krantz, Edda Klipp

{"title":"Moonlighting proteins - an approach to systematize the concept.","authors":"Maria Krantz, Edda Klipp","doi":"10.3233/ISB-190473","DOIUrl":"https://doi.org/10.3233/ISB-190473","url":null,"abstract":"Moonlighting refers to a protein with at least two unrelated, mechanistically different functions. As a concept, moonlighting describes a large and diverse group of proteins which have been discovered in a multitude of organisms. As of today, a systematized view on these proteins is missing. Here, we propose a classification of moonlighting proteins by two classifiers. We use the function of the protein as a first classifier: activating - activating (Type I), activating - inhibiting (Type II), inhibiting - activating (Type III) and inhibiting - inhibiting (Type IV). To further specify the type of moonlighting protein, we used a second classifier based on the character of the factor that switches the function of the protein: external factor affecting the protein (Type A), change in the first pathway (Type B), change in the second pathway (Type C), equal competition between both pathways (Type D). Using a small two-pathway model we simulated these types of moonlighting proteins to elucidate possible behaviors of the types of moonlighting proteins. We find that, using the results of our simulations, we can classify the behavior of the moonlighting types into Blinker, Splitter andSwitch.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-190473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37829807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A computational model of bidirectional axonal growth in micro-tissue engineered neuronal networks (micro-TENNs). 微组织工程神经元网络（micro-TENNs）中轴突双向生长的计算模型。

In Silico Biology Pub Date : 2020-01-01 DOI: 10.3233/ISB-180172

Toma Marinov, Haven A López Sánchez, Liang Yuchi, Dayo O Adewole, D Kacy Cullen, Reuben H Kraft

{"title":"A computational model of bidirectional axonal growth in micro-tissue engineered neuronal networks (micro-TENNs).","authors":"Toma Marinov, Haven A López Sánchez, Liang Yuchi, Dayo O Adewole, D Kacy Cullen, Reuben H Kraft","doi":"10.3233/ISB-180172","DOIUrl":"10.3233/ISB-180172","url":null,"abstract":"Micro-Tissue Engineered Neural Networks (Micro-TENNs) are living three-dimensional constructs designed to replicate the neuroanatomy of white matter pathways in the brain and are being developed as implantable micro-tissue for axon tract reconstruction, or as anatomically-relevant in vitro experimental platforms. Micro-TENNs are composed of discrete neuronal aggregates connected by bundles of long-projecting axonal tracts within miniature tubular hydrogels. In order to help design and optimize micro-TENN performance, we have created a new computational model including geometric and functional properties. The model is built upon the three-dimensional diffusion equation and incorporates large-scale uni- and bi-directional growth that simulates realistic neuron morphologies. The model captures unique features of 3D axonal tract development that are not apparent in planar outgrowth and may be insightful for how white matter pathways form during brain development. The processes of axonal outgrowth, branching, turning and aggregation/bundling from each neuron are described through functions built on concentration equations and growth time distributed across the growth segments. Once developed we conducted multiple parametric studies to explore the applicability of the method and conducted preliminary validation via comparisons to experimentally grown micro-TENNs for a range of growth conditions. Using this framework, the model can be applied to study micro-TENN growth processes and functional characteristics using spiking network or compartmental network modeling. This model may be applied to improve our understanding of axonal tract development and functionality, as well as to optimize the fabrication of implantable tissue engineered brain pathways for nervous system reconstruction and/or modulation.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0a/bb/isb-13-isb180172.PMC7505002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37921296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hybrid multiscale model for investigating tumor angiogenesis and its response to cell-based therapy. 研究肿瘤血管生成及其对细胞治疗反应的混合多尺度模型。

In Silico Biology Pub Date : 2019-01-01 DOI: 10.3233/ISB-170469

Melisa Hendrata, Janti Sudiono

{"title":"A hybrid multiscale model for investigating tumor angiogenesis and its response to cell-based therapy.","authors":"Melisa Hendrata, Janti Sudiono","doi":"10.3233/ISB-170469","DOIUrl":"https://doi.org/10.3233/ISB-170469","url":null,"abstract":"Angiogenesis, a formation of blood vessels from an existing vasculature, plays a key role in tumor growth and its progression into cancer. The lining of blood vessels consists of endothelial cells (ECs) which proliferate and migrate, allowing the capillaries to sprout towards the tumor to deliver the needed oxygen. Various treatments aiming to suppress or even inhibit angiogenesis have been explored. Mesenchymal stem cells (MSCs) have recently been undergoing development in cell-based therapy for cancer due to their ability to migrate towards the capillaries and induce the apoptosis of the ECs, causing capillary degeneration. However, further investigations in this direction are needed as it is usually difficult to preclinically assess the efficacy of such therapy. We develop a hybrid multiscale model that integrates molecular, cellular, tissue and extracellular components of tumor system to investigate angiogenesis and tumor growth under MSC-mediated therapy. Our simulations produce angiogenesis and vascular tumor growth profiles as observed in the experiments. Furthermore, the simulations show that the effectiveness of MSCs in inducing EC apoptosis is density dependent and its full effect is reached within several days after MSCs application. Quantitative agreements with experimental data indicate the predictive potential of our model for evaluating the efficacy of cell-based therapies targeting angiogenesis.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-170469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35239545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Modeling cell population dynamics. 细胞群体动力学建模。

In Silico Biology Pub Date : 2019-01-01 DOI: 10.3233/ISB-180470

Daniel A Charlebois, Gábor Balázsi

引用次数: 44

Lower connectivity of tumor coexpression networks is not specific to cancer. 肿瘤共表达网络的低连通性并不是癌症特有的。

In Silico Biology Pub Date : 2019-01-01 DOI: 10.3233/ISB-190472

Ertuğrul Dalgıç, Özlen Konu, Zehra Safi Öz, Christina Chan

{"title":"Lower connectivity of tumor coexpression networks is not specific to cancer.","authors":"Ertuğrul Dalgıç, Özlen Konu, Zehra Safi Öz, Christina Chan","doi":"10.3233/ISB-190472","DOIUrl":"https://doi.org/10.3233/ISB-190472","url":null,"abstract":"Global level network analysis of molecular links is necessary for systems level view of complex diseases like cancer. Using genome-wide expression datasets, we constructed and compared gene co-expression based specific networks of pre-cancerous tumors (adenoma) and cancerous tumors (carcinoma) with paired normal networks to assess for any possible changes in network connectivity. Previously, loss of connectivity was reported as a characteristics of cancer samples. Here, we observed that pre-cancerous conditions also had significantly less connections than paired normal samples. We observed a loss of connectivity trend for colorectal adenoma, aldosterone producing adenoma and uterine leiomyoma. We also showed that the loss of connectivity trend is not specific to positive or negative correlation based networks. Differential hub genes, which were the most highly differentially less connected genes in tumor, were mostly different between different datasets. No common gene list could be defined which underlies the lower connectivity of tumor specific networks. Connectivity of colorectal cancer methylation targets was different from other genes. Extracellular space related terms were enriched in negative correlation based differential hubs and common methylation targets of colorectal carcinoma. Our results indicate a systems level change of lower connectivity as cells transform to not only cancer but also pre-cancerous conditions. This systems level behavior could not be attributed to a group of genes.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-190472","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37301274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Calibration, Selection and Identifiability Analysis of a Mathematical Model of the in vitro Erythropoiesis in Normal and Perturbed Contexts. 正常和扰动条件下体外红细胞生成数学模型的校准、选择和可识别性分析。

In Silico Biology Pub Date : 2019-01-01 DOI: 10.3233/ISB-190471

Ronan Duchesne, Anissa Guillemin, Fabien Crauste, Olivier Gandrillon

{"title":"Calibration, Selection and Identifiability Analysis of a Mathematical Model of the in vitro Erythropoiesis in Normal and Perturbed Contexts.","authors":"Ronan Duchesne, Anissa Guillemin, Fabien Crauste, Olivier Gandrillon","doi":"10.3233/ISB-190471","DOIUrl":"https://doi.org/10.3233/ISB-190471","url":null,"abstract":"The in vivo erythropoiesis, which is the generation of mature red blood cells in the bone marrow of whole organisms, has been described by a variety of mathematical models in the past decades. However, the in vitro erythropoiesis, which produces red blood cells in cultures, has received much less attention from the modelling community. In this paper, we propose the first mathematical model of in vitro erythropoiesis. We start by formulating different models and select the best one at fitting experimental data of in vitro erythropoietic differentiation obtained from chicken erythroid progenitor cells. It is based on a set of linear ODE, describing 3 hypothetical populations of cells at different stages of differentiation. We then compute confidence intervals for all of its parameters estimates, and conclude that our model is fully identifiable. Finally, we use this model to compute the effect of a chemical drug called Rapamycin, which affects all states of differentiation in the culture, and relate these effects to specific parameter variations. We provide the first model for the kinetics of in vitro cellular differentiation which is proven to be identifiable. It will serve as a basis for a model which will better account for the variability which is inherent to the experimental protocol used for the model calibration.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-190471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37172496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calibration, Selection and Identifiability Analysis of a Mathematical Model of the in vitro Erythropoiesis in Normal and Perturbed Contexts 正常和扰动条件下体外红细胞生成数学模型的校准、选择和可识别性分析

In Silico Biology Pub Date : 2018-05-05 DOI: 10.1101/314955

Ronan Duchesne, Anissa Guillemin, F. Crauste, O. Gandrillon

{"title":"Calibration, Selection and Identifiability Analysis of a Mathematical Model of the in vitro Erythropoiesis in Normal and Perturbed Contexts","authors":"Ronan Duchesne, Anissa Guillemin, F. Crauste, O. Gandrillon","doi":"10.1101/314955","DOIUrl":"https://doi.org/10.1101/314955","url":null,"abstract":"The in vivo erythropoiesis, which is the generation of mature red blood cells in the bone marrow of whole organisms, has been described by a variety of mathematical models in the past decades. However, the in vitro erythropoiesis, which produces red blood cells in cultures, has received much less attention from the modelling community. In this paper, we propose the first mathematical model of in vitro erythropoiesis. We start by formulating different models and select the best one at fitting experimental data of in vitro erythropoietic differentiation. It is based on a set of linear ODE, describing 3 hypothetical populations of cells at different stages of differentiation. We then compute confidence intervals for all of its parameters estimates, and conclude that our model is fully identifiable. Finally, we use this model to compute the effect of a chemical drug called Rapamycin, which affects all states of differentiation in the culture, and relate these effects to specific parameter variations. We provide the first model for the kinetics of in vitro cellular differentiation which is proven to be identifiable. It will serve as a basis for a model which will better account for the variability which is inherent to experimental protocol used for the model calibration.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43637124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Simulation of diffusion using a modular cell dynamic simulation system. 用模块化单元动态模拟系统模拟扩散。

In Silico Biology Pub Date : 2017-01-01 DOI: 10.3233/ISB-170468

Christoph Leberecht, Florian Heinke, Dirk Labudde

引用次数: 4

Differential transcriptional regulation by alternatively designed mechanisms: A mathematical modeling approach. 不同设计机制的差异转录调控:数学建模方法。

In Silico Biology Pub Date : 2017-01-01 DOI: 10.3233/ISB-160467

Necmettin Yildirim, Mehmet Emin Aktas, Seyma Nur Ozcan, Esra Akbas, Ahmet Ay

{"title":"Differential transcriptional regulation by alternatively designed mechanisms: A mathematical modeling approach.","authors":"Necmettin Yildirim, Mehmet Emin Aktas, Seyma Nur Ozcan, Esra Akbas, Ahmet Ay","doi":"10.3233/ISB-160467","DOIUrl":"https://doi.org/10.3233/ISB-160467","url":null,"abstract":"Cells maintain cellular homeostasis employing different regulatory mechanisms to respond external stimuli. We study two groups of signal-dependent transcriptional regulatory mechanisms. In the first group, we assume that repressor and activator proteins compete for binding to the same regulatory site on DNA (competitive mechanisms). In the second group, they can bind to different regulatory regions in a noncompetitive fashion (noncompetitive mechanisms). For both competitive and noncompetitive mechanisms, we studied the gene expression dynamics by increasing the repressor or decreasing the activator abundance (inhibition mechanisms), or by decreasing the repressor or increasing the activator abundance (activation mechanisms). We employed delay differential equation models. Our simulation results show that the competitive and noncompetitive inhibition mechanisms exhibit comparable repression effectiveness. However, response time is fastest in the noncompetitive inhibition mechanism due to increased repressor abundance, and slowest in the competitive inhibition mechanism by increased repressor level. The competitive and noncompetitive inhibition mechanisms through decreased activator abundance show comparable and moderate response times, while the competitive and noncompetitive activation mechanisms by increased activator protein level display more effective and faster response. Our study exemplifies the importance of mathematical modeling and computer simulation in the analysis of gene expression dynamics.","PeriodicalId":39379,"journal":{"name":"In Silico Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/ISB-160467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34628179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1