{"title":"How and Why to Teach English Metaphors to Japanese Learners Through Visual Media: With Practical Examples From Steven Universe","authors":"Spring, Ryan, Suarez, Gerard","doi":"10.16875/stem.2018.19.4.109","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.109","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"317 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75454945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Analysis of English Vocabulary Based on Corpora From the Korean and American Versions of The Good Doctor","authors":"고성란","doi":"10.16875/stem.2018.19.4.27","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.27","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"182 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75674630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Study on the Validity of the English Learning Style Scale for Korean High School Students in a Native Teacher’s Class Based on Multimedia","authors":"Nayu Kim, 이희철","doi":"10.16875/stem.2018.19.4.149","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.149","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90446850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Case Study on the Contents Creation of Online Lectures to Promote Interest and Concentration of Learners","authors":"Hye-Kyung Kang","doi":"10.16875/STEM.2018.19.4.127","DOIUrl":"https://doi.org/10.16875/STEM.2018.19.4.127","url":null,"abstract":",","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88126262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring Intercultural Communication Themes in English Classes Through the Movie Outsourced","authors":"Andrea Lee","doi":"10.16875/stem.2018.19.4.45","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.45","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"283 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Inferencing Tasks on Low-Proficiency Korean EFL Learners’ Learning Vocabulary From Movies","authors":"Lee, Jin Hyung","doi":"10.16875/stem.2018.19.4.71","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.71","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"71 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72453403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effectiveness of Using Films and Documentaries in the EAP Classroom to Teach Contemporary Political Discourse","authors":"Kim, Kitai","doi":"10.16875/stem.2018.19.4.1","DOIUrl":"https://doi.org/10.16875/stem.2018.19.4.1","url":null,"abstract":"","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85110802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kamal, Deded Yudha Pranatha, Waluyo Sugito, F. Rahman, Eka Susanto, S. Mariya, Wei Ming Chen
{"title":"Isolation, Culture and Characterization of Cancer Stem Cells from Primary Osteosarcoma","authors":"A. Kamal, Deded Yudha Pranatha, Waluyo Sugito, F. Rahman, Eka Susanto, S. Mariya, Wei Ming Chen","doi":"10.2174/1876893801805010001","DOIUrl":"https://doi.org/10.2174/1876893801805010001","url":null,"abstract":"Osteosarcoma cancer stem cells (CSCs) are defined as a subpopulation of osteosarcoma cells, which have the ability of self-renewal, proliferation and differentiation. This study aimed to identify CSCs from human osteosarcomain vitro.Osteosarcoma CSCs were isolated and cultured with sphere-forming assay technique on an ultra-low well attachment surface plate. After sarcosphere colonies were formed, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the expression of genes of embryonic stem cells such asNANOG, Oct3/4, STAT3 and gene of MSC CD133. Immunofluorescence analysis (IFA) of alkaline phosphatase (ALP), osteocalcin, and CD 133 was also performed to see the expression of osteosarcoma CSC surface protein with immuno-enzymatic staining principle. We also performed alizarin red staining to evaluate calcification in osteosarcoma CSCs.The culture sphere-of the osteosarcoma cells showed three dimension round shaped colonies (sarcospheres) in slightly hypoxicand serum free condition which was not attached to the substrate with tight density. RT-PCR demonstrated that sarcospheres expressed genes which encodeNANOG, Oct3/4 STAT 3, but not for CD 133. IFA showed positive protein expression of ALP, osteocalcin and CD 133 which was moderate, strong, and weak positive respectively. Sarcospheres also had a positive reaction toward alizarin red staining.Osteosarcoma CSCs could be isolated from human osteosarcoma by sphere-forming assay technique and characterized by the expression of genes of embryonic stem cells,such asNANOG, Oct3/4, STAT3 and IFA of ALP, osteocalcin, and CD 133.","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48550834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"영화를 기반으로 한 영어 향상 방안","authors":"서은미","doi":"10.16875/stem.2018.19.3.45","DOIUrl":"https://doi.org/10.16875/stem.2018.19.3.45","url":null,"abstract":"The purpose of this paper is to show the effect of cinema-based learning for EFL college students. Constructive learning theory maintains that...","PeriodicalId":38955,"journal":{"name":"Open Stem Cell Journal","volume":"6 1","pages":"45-64"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80322169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}