{"title":"Extraction, Separation and Purification of Acidic Polysaccharide from Morchella esculenta by High Voltage Pulsed Electric Field","authors":"Chao Liu","doi":"10.7546/IJBA.2019.23.2.000678","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000678","url":null,"abstract":"The intracellular acidic polysaccharide from Morchella esculenta mycelium produced by submerged fermentation is extracted and separated. In the process of extracting the polysaccharide by the high voltage pulsed electric field (HVPEF), heat is not generated and the structure of the polysaccharide is not destroyed. The response surface method is used to investigate the electric field intensity, the pulse number and the liquid-to-material ratio. The optimum extraction process of the acidic polysaccharide is obtained as follows: the electric field intensity is 18 kV/cm, the pulse number is 7 and the liquid-to-material ratio is 27 mL/g. At this time, the polysaccharide yield reaches a maximum value of 56.03 μg/mL. The crude polysaccharide is separated and purified by using DEAE-52 cellulose ion exchange column and Sephadex G-100 to obtain an acidic polysaccharide F1 with high purity. The molecular weight is 1.9895×10 Da. Its functional and structural characteristics need to be further studied.","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43636205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of Substrate and Biomass Concentrations in a Chemostat using an Extended Kalman Filter","authors":"Oussama Hadj-Abdelkader, Amine Hadj-Abdelkader","doi":"10.7546/IJBA.2019.23.2.000551","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000551","url":null,"abstract":"This paper presents the estimation of substrate and biomass concentrations inside a Chemostat used for waste-water treatment. These concentrations represent the state variables of the process model. Most research in this field used only deterministic models, not accounting for uncertainties and noises on the states and on the output. Hence, the estimation of these concentrations may not be sufficiently accurate. For a more realistic description, we used here a stochastic formulation. Unlike the other research works, we used a stochastic differential equations (SDE) model which provides a better representation of the system in his natural processing scale. This model also includes the aleatory effects in the process which had been discarded in the other works. We then deal with the state estimation problem using an Extended Kalman filter, which proceeds with a linearization of the model around a deterministic trajectory. The classical prediction and update steps of the filter are then carried-out and led to good results. Notice that the system in study has some interesting properties such as discrete-time observations, high noise intensities and slow-time evolution. Results are presented, discussed and compared with the related state-of-the-art researches.","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44959357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dynamics of Changes in Visual Evoked Potentials Values with the Advance of Retinal Changes in Patients with Diabetes Mellitus","authors":"E. Mermeklieva","doi":"10.7546/IJBA.2019.23.2.000639","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000639","url":null,"abstract":"Aim: The aim of the study was to explore objectively the visual analyzer (VA) function by pattern visual evoked potentials (PVEPs) in patients with diabetes mellitus (DM) in different stages of diabetic retinopathy (DR) and to compare the results with controls and between the different groups. Material and methods: This is a prospective observation study with 3-year duration (20142017). A group of 185 people was studied. Patients with DM were 138. They were divided into two main groups patients without DR and patients with DR. The first group consisted of two subgroups – patients with newly-diagnosed DM (33 people) and patients with DM duration longer than 1 year (mean DM duration 6.8 ± 4.2 years) (36 people). The second main group consists of patients with DR, divided into two subgroups – patients with initial DR (34 people) and patients with advanced DR (35 people). Controls for the EF studies were 47 healthy individuals. PVEPs were performed. The main variables that were considered in the results analysis were the latency and amplitude, reflecting the configuration of the wave forms. Results: PVEPs results were affected even in patients without DR. The changes in PVEPs values became more distinct in patients with initial DR group as а number of components with significant difference as well as a degree of significant difference, to reach their maximum number and significance manifestation peak in patients with advanced DR, the most affected by DM group. Conclusion: PVEPs studies could be used as an objective methods for registration of early changes in the VA function as a DM complication. Also, to monitor the changes in dynamics as they are non-invasive, harmless, fast, inexpensive and repeatable.","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42320197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Intuitionistic Fuzzy Sets for Conflict Resolution Modeling and Agent Based Simulation","authors":"Shpend Ismaili, S. Fidanova","doi":"10.7546/IJBA.2019.23.2.000544","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000544","url":null,"abstract":"Very topical nowadays is the modeling and simulation of conflict situations. The aim is their peaceful prevention and conflict resolution. Different mathematical approaches are used. One of them is the application of multi-agent systems. The multiagent system consists of software agents, which are autonomous subjects working together. In our application the agents represents police officers and civilians in case of protest. In this work we propose application of intuitionistic fuzzy sets for representation of the movement and interaction of the agents. The intuitionistic fuzzy sets are extension of the fuzzy sets including level of uncertainty. Thus the model becomes more realistic. The conflict situation can cause disturbances and casualties. Crowd simulation is very difficult and important research topic.","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46898450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Manually Defined Continuous Positive Airway Pressure versus Mathematically Calculated for Obstructive Sleep Apnea","authors":"Krassimir Rankov, N. Zaekov, M. Nikolova","doi":"10.7546/IJBA.2019.23.2.000523","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000523","url":null,"abstract":"","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41566552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Numerical Study on a Model for HIV Infection of CD4+T-cells by Shifted Chebyshev Polynomials","authors":"K. Parand, M. M. Moayeri, S. Latifi","doi":"10.7546/IJBA.2019.23.2.000498","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000498","url":null,"abstract":"","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42975741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veselina Bureva, Stanislav Popov, V. Traneva, S. Tranev
{"title":"Generalized Net Model of Cluster Analysis Using CLIQUE: Clustering in Quest","authors":"Veselina Bureva, Stanislav Popov, V. Traneva, S. Tranev","doi":"10.7546/IJBA.2019.23.2.000506","DOIUrl":"https://doi.org/10.7546/IJBA.2019.23.2.000506","url":null,"abstract":"The purpose of the cluster analysis is to find groups of objects with similar characteristics. Different methods are already developed. In the current research work a CLIQUE: Clustering in quest algorithm is investigated. The presented method combines subspace grid-based and density-based techniques to determine clusters of objects. Generalized net of cluster analysis using CLIQUE: Clustering in quest algorithm is constructed. The presented Generalized net can be used for description and monitoring the parallel processes in the cluster analysis.","PeriodicalId":38867,"journal":{"name":"International Journal Bioautomation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44981611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}