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PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 蜡样芽孢杆菌HSFI-10蛋白酶的生产优化、部分纯化及溶栓活性评价
Biotropia Pub Date : 2023-08-01 DOI: 10.11598/btb.2023.30.2.1765
Ainutajriani Ainutajriani, S. Darmawati, D. S. Zilda, M. Afriansyah, Ragil Saptaningtyas, S. Ethica
{"title":"PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10","authors":"Ainutajriani Ainutajriani, S. Darmawati, D. S. Zilda, M. Afriansyah, Ragil Saptaningtyas, S. Ethica","doi":"10.11598/btb.2023.30.2.1765","DOIUrl":"https://doi.org/10.11598/btb.2023.30.2.1765","url":null,"abstract":"Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89549970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
INDIGENOUS BACILLUS SPECIES ISOLATED FROM AEDES AEGYPTI LARVAE: ISOLATION, LARVICIDAL TOXICITY SCREENING, PHENOTYPIC CHARACTERIZATION, AND MOLECULAR IDENTIFICATION 从埃及伊蚊幼虫中分离的原生芽孢杆菌:分离、杀幼虫毒性筛选、表型表征和分子鉴定
Biotropia Pub Date : 2023-08-01 DOI: 10.11598/btb.2023.30.2.1938
Salamun, R. D. Susetyo, Hakimatul Husniyah, Almando Geraldi, NI’MATUZ Ahroh, Farah Aisyah Nafidiastri, Nabilatun Nisa', Muhammad Fath Alhaqqi, Sanis Salamy
{"title":"INDIGENOUS BACILLUS SPECIES ISOLATED FROM AEDES AEGYPTI LARVAE: ISOLATION, LARVICIDAL TOXICITY SCREENING, PHENOTYPIC CHARACTERIZATION, AND MOLECULAR IDENTIFICATION","authors":"Salamun, R. D. Susetyo, Hakimatul Husniyah, Almando Geraldi, NI’MATUZ Ahroh, Farah Aisyah Nafidiastri, Nabilatun Nisa', Muhammad Fath Alhaqqi, Sanis Salamy","doi":"10.11598/btb.2023.30.2.1938","DOIUrl":"https://doi.org/10.11598/btb.2023.30.2.1938","url":null,"abstract":"Vector-borne diseases transmitted by mosquitoes are considered a significant public health problem worldwide. Aedes aegypti is one of the mosquito species responsible for transmitting these diseases. One environmentally friendly method of vector control is the use of microbial agents such as Bacillus species. This study aimed to explore investigate indigenous entomopathogenic bacteria of Bacillus species isolated from A. aegypti larvae. Larvae samples were collected from breeding sites of A. aegypti. All isolates underwent screening and affirmation confirmation tests to assess their larvicidal toxicity against A. aegypti larvae. Phenotypic characterizations and molecular identifications were conducted to determine the species of the Bacillus isolates based on similarity index and percent identity (%ID). Phylogenetic trees were used to compare the isolates with other Bacillus species. The results revealed 120 isolates of Bacillus species from A. aegypti larvae samples. Among them, three isolates (LS3.3, LS9.1, and LSD4.2) exhibited the highest larvicidal toxicity in the confirmation test, resulting in larval mortality rates of 100%, 96.7%, and 100%, respectively, after 48 hours of exposure. Molecular identifications, showed that LSD4.2 had a 99.16% ID with Bacillus velezensis, LS3.3 had a 98.22% ID with Bacillus mojavensis, and LS9.1 had a 99.93% ID with Bacillus subtilis. These three bacteria from the Bacillus genus have been reported to offer significant benefits to humans.","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"2005 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86970868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OPTIMIZATION OF Talinum paniculatum Gaertn. ROOT INDUCTION AND THE EFFECT OF PHOSPHATE CONCENTRATIONS AND AMMONIUM:NITRATE RATIO ON BIOMASS OF ADVENTITIOUS ROOTS IN IN VITRO CULTURE 薤白的优选。根系诱导及磷浓度和铵硝比对离体培养不定根生物量的影响
Biotropia Pub Date : 2023-08-01 DOI: 10.11598/btb.2023.30.2.1678
Palupi Dasawulan, S. Lestari, Putri Fajrisani, Sugiharto Yosephine Gehasti, Sri Wulan, Manuhara
{"title":"OPTIMIZATION OF Talinum paniculatum Gaertn. ROOT INDUCTION AND THE EFFECT OF PHOSPHATE CONCENTRATIONS AND AMMONIUM:NITRATE RATIO ON BIOMASS OF ADVENTITIOUS ROOTS IN IN VITRO CULTURE","authors":"Palupi Dasawulan, S. Lestari, Putri Fajrisani, Sugiharto Yosephine Gehasti, Sri Wulan, Manuhara","doi":"10.11598/btb.2023.30.2.1678","DOIUrl":"https://doi.org/10.11598/btb.2023.30.2.1678","url":null,"abstract":"Java ginseng (Talinum paniculatum Gaertn.) is a medicinal plant, the roots of which are commonly used in traditional medicine. In its natural habitat, the roots grow very slowly, requiring two to three years to produce 100 g of roots per plant. Plant tissue culture could therefore provide an alternative means of accelerating root growth. This research aimed to optimize root induction and determine the effect of phosphate (KH2PO4) concentration and the ratio between ammonium and nitrate (ammonium:nitrate) on the biomass of Java ginseng adventitious roots in in vitro culture. Stem and leaf were used as explants and various combinations and concentrations of IBA and BAP, kinetin, and TDZ were used as growth regulators. Leaf explants were grown in Murashige and Skoog (MS) media supplemented with IBA 2 mg/L and various concentrations of phosphate (170; 212.5; 255; 297.5; 340; 382.5; 425; 467.5; 510 mg/L) and various ammonium:nitrate ratios (21:19 mM as the control, 0:30 mM, 10:20 mM, 15:15 mM, 20:10 mM, 30:0 mM). Cultures were maintained for 6 weeks. The observed parameters were fresh weight, dry weight, the duration of root formation, and the number and length of adventitious roots. The data were analyzed using Analysis of Variance. The results showed that the concentration of phosphate and the ammonium:nitrate ratio significantly influenced the amount, length, fresh weight, and dry weight of Java ginseng adventitious root. The highest fresh weight (37.47 mg) and dry weight (5.53 mg) were achieved in the treatment of double phosphate concentration (KH2PO4 340 mg/L), while an ammonium:nitrate ratio of 10:20 mM was the optimum treatment to produce the highest biomass (fresh weight 73.6 mg and dry weight 8.2 mg).","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83287998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DIVERSITY, ECOLOGY AND CONSERVATION STATUS OF NEPENTHES IN WEST SUMATRA PROVINCE, INDONESIA 印度尼西亚西苏门答腊省新戊草的多样性、生态学和保护现状
Biotropia Pub Date : 2023-08-01 DOI: 10.11598/btb.2023.30.2.1896
M. Mansur, A. Salamah, E. Mirmanto, F. Brearley
{"title":"DIVERSITY, ECOLOGY AND CONSERVATION STATUS OF NEPENTHES IN WEST SUMATRA PROVINCE, INDONESIA","authors":"M. Mansur, A. Salamah, E. Mirmanto, F. Brearley","doi":"10.11598/btb.2023.30.2.1896","DOIUrl":"https://doi.org/10.11598/btb.2023.30.2.1896","url":null,"abstract":"Nepenthes is the largest carnivorous plant genus present in Indonesia. There are 39 species of Nepenthes pitcher plants recorded in Sumatra from lowland to montane forests, and 34 of them are endemic; this represents the greatest species diversity of Nepenthes after Borneo. Field studies were conducted in 2021 and 2022 to increase our knowledge of the diversity, habitats and distributions of Nepenthes in West Sumatra province. Twenty-three species of Nepenthes were recorded from the province, consisting of 15 highland species, 4 mid-elevation species and 4 lowland species. Ecophysiological studies conducted at Bukit Malalak showed clumped distributions of N. bongso, N. dubia, N. eustachya and N. rhombicaulis. Foliar and pitcher fluid nutrient concentrations were found to be similar to those cited in other recent studies although growth rates were slightly more rapid than at Gunung Talang. Bukit Malalak is a new locality for two threatened species, namely N. dubia (CR) and N. rhombicaulis (VU), enlarging their extents of occurrence. In total, nine species from West Sumatra are threatened and conservation actions are urgently needed for these and other Nepenthes species remaining on the island.","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74301284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MOLECULAR DOCKING ANALYSIS OF SELECTED CURCUMA XANTHORRHIZA CONSTITUENTS AS POTENTIAL ANTICANCER DRUG 姜黄黄根成分潜在抗癌药物的分子对接分析
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1386
Akhmad endangzainal Hasan
{"title":"MOLECULAR DOCKING ANALYSIS OF SELECTED CURCUMA XANTHORRHIZA CONSTITUENTS AS POTENTIAL ANTICANCER DRUG","authors":"Akhmad endangzainal Hasan","doi":"10.11598/btb.2023.30.1.1386","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1386","url":null,"abstract":"Stress conditions will trigger the histone hyperacetylation process due to histone acetyltransferase p300/CBP (HAT PCAF) constantly transfers acetyl groups from acetyl-CoA to conserved lysine residues on histone proteins to form ε-N-acetyllysine. This can be a cause of cancer. The purpose of this study was to investigate the potential mechanisms and inhibition of PCAF HAT by chemical components of C. xanthorrhiza namely, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and xanthorrizhol using in silico, the molecular docking method.  Results showed that the components of C. xanthorrhiza as ligands have the capability to inhibit the binding of acetyl-CoA to histone. These results can be used to predict the inhibitory mechanisms exhibited by C. xanthorrhiza components, as competitive and noncompetitive substances. We hypothesize that C. xanthorrhiza components resemble a substrate, leading to prevention of the natural substrate (histone) to bind to the enzyme, and hence block the product formation. The smallest free Gibs energy was exhibited by curcumin on chain B and by bismethoxycurcumin on chain A, with values of -8.8 and -8.4 Kcal/mol, respectively.Natural ","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78787259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
POTENCY OF YEAST AS A BIOCONTROL AGENT OF OCHRATOXIN A-PRODUCING FUNGI AND ITS EFFECT ON ARABICA COFFEE TASTE 酵母作为产赭曲霉毒素a真菌的防效及其对阿拉比卡咖啡口感的影响
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1379
O. S. Dharmaputra, I. Retnowati, N. Nurfadila
{"title":"POTENCY OF YEAST AS A BIOCONTROL AGENT OF OCHRATOXIN A-PRODUCING FUNGI AND ITS EFFECT ON ARABICA COFFEE TASTE","authors":"O. S. Dharmaputra, I. Retnowati, N. Nurfadila","doi":"10.11598/btb.2023.30.1.1379","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1379","url":null,"abstract":"Biocontrol agents can be used to control mycotoxigenic fungi, which include different species of yeast. The objectives of this research were to select yeast isolates that can inhibit the growth of ochratoxin A (OTA)-producing fungi (Aspergillus ochraceus BIO 37310) and to increase the taste of Arabica coffee processed using wet and semi-wet methods. Twenty-two yeast isolates (KA, KA2, KB, KB2, KC, KD, Endomyces decipiens BIO 131215, E. fibuliger BIO 132216, BIO 132217, BIO 13218, BIO 132219, BIO 132220, Candida krusei (= Issatchenkia orientalis) BIO 211285, BIO 211286, BIO 211287, BIO 211288, BIO 211289, BIO 211290, BIO 211291, Saccharomyces cerevisiae BIO 341363, BIO 341364, and BIO 341365) were screened for their antagonistic property against A. ochraceus BIO 37310 in vitro using well (dip) test method. The results showed that C. krusei (BIO 211287, BIO 211288, and BIO 211289) inhibited A. ochraceus BIO 37310. In vivo the highest yeast population was found in coffee beans processed using a semi-wet method inoculated with C. krusei BIO 211288 (46,222 ± 9,576 cfu/g), which was not significantly different from that of the coffee beans inoculated with C. krusei BIO 211287 (36,333 ± 14,000 cfu/g). The three yeast isolates were also able to grow either in coffee beans processed using wet or semi-wet methods inoculated with A. ochraceus BIO 37310 and each yeast isolate. Interaction between the three yeast isolates and A. ochraceus BIO 37310 resulted in E-type interaction, i.e. the fungus was not able to grow anymore, while the yeasts grew further. The total cupping scores of coffee beans inoculated with the three yeast isolates were higher than those of coffee beans uninoculated and inoculated with commercial lactic acid bacteria. The three yeast isolates could be used as biocontrol agents of A. ochraceus BIO 37310 and increase the sensorial quality of coffee beverages.","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"142 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76795187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TEA SAPONIN MODULATES IN VITRO RUMEN FERMENTATION PROFILE AND REDUCES METHANE PRODUCTION: A META-ANALYSIS 茶皂素调节体外瘤胃发酵剖面并减少甲烷产生:一项荟萃分析
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1805
Y. R. Yanza, A. Jayanegara, A. Fitri, C. Hidayat
{"title":"TEA SAPONIN MODULATES IN VITRO RUMEN FERMENTATION PROFILE AND REDUCES METHANE PRODUCTION: A META-ANALYSIS","authors":"Y. R. Yanza, A. Jayanegara, A. Fitri, C. Hidayat","doi":"10.11598/btb.2023.30.1.1805","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1805","url":null,"abstract":"Saponins are secondary plant metabolites binding hydrophobic non-sugar components that can reduce ruminant methane (CH4) production. Previous studies revealed that tea saponin is one of the common sources of saponins used to reduce enteric CH4 production. However, the effect of different levels of dietary tea saponin on rumen fermentation remains inconclusive. This study aimed to investigate the effect of tea saponin on in vitro rumen fermentation and methane production through a meta-analysis approach. A total of 6 articles were selected and included in the meta-analysis database, continued by the statistical analysis using SAS software through mixed model formula. The results showed that tea saponin levels lowered in vitro organic matter degradability (IVOMD) and in vitro dried matter degradability (IVDMD; P=0.01) by a linear response. Furthermore, increased tea saponin levels in the diet reduced enteric CH4 emission expressed as CH4/DM substrate and CH4/IVOMD by a quadratic (P=0.01) and a linear response (P=0.04), respectively. Increased tea saponin levels also tended to decrease gas production linearly (P=0.08). Tea saponins inclusion also decreased pH levels linearly (P=0.04) but increased NH3 quadratically (P=0.01). Moreover, total VFA concentration in the rumen also raised quadratically, followed by the increased acetate (C2) and propionate (C3) proportion by a linear response (P<0.05). As a result, the C2/C3 ratio was decreased in a linear response (P=0.01) by the increased tea saponin levels, where the protozoa population was also diminished by a quadratic response (P=0.01). In conclusion, tea saponin levels directly reduced enteric CH4 production by diminishing microbial populations. ","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"24 11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82682689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PUERTO PRINCESA SUBTERRANEAN RIVER NATIONAL PARK-MARINE TURTLE CONSERVATION PROGRAM: CHALLENGES, AND OPPORTUNITIES DURING PANDEMIC 公主港地下河国家公园-海龟保护计划:大流行期间的挑战与机遇
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1915
Liwayway H. Acero
{"title":"PUERTO PRINCESA SUBTERRANEAN RIVER NATIONAL PARK-MARINE TURTLE CONSERVATION PROGRAM: CHALLENGES, AND OPPORTUNITIES DURING PANDEMIC","authors":"Liwayway H. Acero","doi":"10.11598/btb.2023.30.1.1915","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1915","url":null,"abstract":"The Puerto Princesa Subterranean River National Park (PPSRNP)-Marine Turtle Conservation Program (MTCP) can be found in West Coast of Palawan Philippines. Geographical location is located in the global center of marine biodiversity, the Coral Triangle. This study described the profile of PPSNRP-MTCP in terms of location, species of marine turtles, number of hatchlings released per year, management organizational structure, conservation activities and, determined the perceived management challenges and opportunities encountered by employees during COVID 19 pandemic. Documentary analysis, net-notgraphy and focal group virtual discussion and structured questionnaires were used in data gathering. There are 3 species that were recorded in the conservation site; Chelonia mydas, Eretmochelys imbricata and Lepidochelys olivacea. Hatchling success from 2015 to 2021 is at an average of 82%, and the mean hatchlings per year is 1659.4 (+/- sd.699.31). Lowest was 697 in 2014-2015, highest in 2019-2020 with 2,476 hatchlings. MTCP is under the supervision of Protected Area Superintendent (PASu). The management activities are; regular monitoring of egg nesting and hatching of the eggs, implementation of easement zone, community coastal clean-up, releasing of hatchlings and conservation awareness campaign. Debris left by typhoon Odette is the perceived very high challenge while the absence of noise during nesting season is perceived at a very high opportunity.","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"374 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76758580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carbon Stored on Seagrass Beds in Gili Maringkik, Lombok, Indonesia 印尼龙目岛Gili Maringkik海草床上储存的碳
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1776
F. Rahman, I. Qayim, Y. Wardiatno
{"title":"Carbon Stored on Seagrass Beds in Gili Maringkik, Lombok, Indonesia","authors":"F. Rahman, I. Qayim, Y. Wardiatno","doi":"10.11598/btb.2023.30.1.1776","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1776","url":null,"abstract":"Seagrass beds are an important vegetation for reducing the impact of global warming besides the function of forest and mangroves vegetation. The research aims to find the composition of seagrass species, density, seagrass coverage, seagrass standing biomass, seagrass standing carbon and substrate carbon. The research was conducted in Gili Maringkik, Lombok, Indonesia. The research included observation of species composition and the sample was collected from 0.5 ×0.5 m plot area. Total plot area was 25 on five lanes with the space between plots 25 m and between lanes 100 m. The researcher found that there were eight seagrass species (two families, six genera): Cymodocea rotundata, Cymodocea serrulata, Enhalus acoroides, Halodule pinifolia, Halophila minor, Halophila spinulosa, Syringodium isoetifolium and Thalassia hemprichii. Cymodocea rotundata (506.40±187.809 stand.m-2) was the species of highest density and while the species of Thalassia hemprichii (36.52±30.004 %) was the species of highest coverage. Total of seagrass standing biomass in Gili Maringkik was 1081.85 g.DW.m-2 with the carbon stock of seagrass beds was 483.86 g.C.m-2 and substrate carbon content was 0.09%–0.49%. the total carbon stock of seagrass beds in Gili Maringkik has 153.96 ton.C (4.84 ton.C.Ha-1).","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80708305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
POLLINATION ECOLOGY, BREEDING SYSTEMS AND SEED DISPERSAL IN PASSIFLORA FOETIDA L. (PASSIFLORACEAE), A PERENNIAL HERBACEOUS CLIMBER WEED IN SOUTHERN PARTS OF ANDHRA PRADESH, INDIA. 印度安得拉邦南部多年生草本攀援植物西番莲的授粉生态学、繁殖系统和种子传播。
Biotropia Pub Date : 2023-04-17 DOI: 10.11598/btb.2023.30.1.1675
Mallikarjuna Rao Medabalimi
{"title":"POLLINATION ECOLOGY, BREEDING SYSTEMS AND SEED DISPERSAL IN PASSIFLORA FOETIDA L. (PASSIFLORACEAE), A PERENNIAL HERBACEOUS CLIMBER WEED IN SOUTHERN PARTS OF ANDHRA PRADESH, INDIA.","authors":"Mallikarjuna Rao Medabalimi","doi":"10.11598/btb.2023.30.1.1675","DOIUrl":"https://doi.org/10.11598/btb.2023.30.1.1675","url":null,"abstract":"Passiflora foetida L. (Passifloraceae) is an herbaceous climber weed; grows up to 1.5 - 6.5m tall occur in riverbeds, wastelands, forest floors, crop fields, and roadsides, ascend about 4 to 10 m (13 to 32 feet) tall, exhibit peak flowering in July to December; produce white flowers marked with a ring of radially oriented purple streaks nectar guide, the flower opens along with sunrise and closes the same day noon. It exhibits three kinds of stigma positions in the same plant with different frequencies which determined the fertility rate of flower 1. Stigma below the anthers 64% (fully curved), 2. Stigmas above the anthers 16% (no curvature), 3. Stigmas above anthers 20% (partially curved). P. foetida flower shows both self-pollination and Cross-pollination. Xylocopa latipas sp. visits the flowers frequently among all of the forages and the Stigmas curved below the anthers produce height fruit set (68%) and seed set (82.14%). The fruits and seeds spread far destinations by animals (Zoochory). It cultivates in agricultural fields for their ornamental flowers, edible fruits, and pharmaceutical uses. The present study explored ecological importance, reproductive ecology and the pollinator interaction of the plant.            \u0000  \u0000Keywords \u0000  \u0000herbaceous climber; fertility rate; self-pollination; cross-pollination; fruit set, seed set; edible fruit. ","PeriodicalId":38783,"journal":{"name":"Biotropia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76020075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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