Walailak Journal of Science and Technology最新文献_第2页

Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues 橡胶皮及其他农业废弃物中潜在木质纤维素降解微生物的分离与筛选

Walailak Journal of Science and Technology Pub Date : 2017-06-15 DOI: 10.14456/VOL15ISS6PP%P

Phat Sakpetch, A. H-Kittikun, A. Chandumpai

{"title":"Isolation and Screening of Potential Lignocellulolytic Microorganisms from Rubber Bark and Other Agricultural Residues","authors":"Phat Sakpetch, A. H-Kittikun, A. Chandumpai","doi":"10.14456/VOL15ISS6PP%P","DOIUrl":"https://doi.org/10.14456/VOL15ISS6PP%P","url":null,"abstract":"Lignocellulolytic microorganisms were isolated from cow manure, the soil from naturally grown bamboo, the piles of rubber bark and the composting piles. The samples were collected at 9 different locations which were 5 cm deep from the surface. The basal medium (BM) containing CMC (carboxymethyl cellulose) or xylan as a carbon source and the lignin modifying basal medium (LBM) with ABTS {2,2¢-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid} or DMP (2,6-dimethoxyphenol) as an inducer were used for the primary screening of microorganisms producing cellulase, xylanase, laccase and manganese peroxidase, respectively. Forty-five bacterial isolates (B), twenty-five isolates of actinomyces (A) and fifteen fungal isolates (F) were obtained. After that twelve isolates were selected to evaluate enzyme production in LBM containing decomposed rubber bark as a carbon source at 30 °C and at 45 °C under aerobic conditions (150 rpm shaking). The isolates F6, A2 and B15 displayed the highest enzyme activities with a CMCase activity of 0.61, 0.33 and 0.21 U/mL, respectively. Moreover, they also produced xylanase with an activity of 0.61, 0.36 and 0.32 U/mL, respectively. However, only the isolate F6 showed laccase activity and manganese peroxidase activity of 0.81 and 0.56 U/mL, respectively. The isolates B15 and A2 were identified by 16S rDNA with 99 % similarity to Bacillus subtilis and Streptomyces thermovulgaris , respectively. Aside from that, isolate F6 was identified by 18S rDNA with 100 % similarity to Trichoderma asperellum . When these microorganisms were grown in the LBM broth with 10 % decomposed rubber bark they produced the highest lignocellulolytic enzymes in the stationary phase of growth.","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"14 1","pages":"953-967"},"PeriodicalIF":0.0,"publicationDate":"2017-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66681014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Modeling Network Evolution by Colored Petri Nets 基于有色Petri网的网络演化建模

Walailak Journal of Science and Technology Pub Date : 2016-11-09 DOI: 10.14456/VOL14ISS10PP%P

Suwimon Vongsingthong, Sirapat Boonkrong, H. Unger

引用次数: 2

In Vitro Laticifer Identification in Young Shoot-Derived Callus of Hevea brasiliensis Muell. Arg. 巴西橡胶树幼芽愈伤组织乳汁管的体外鉴定。参数。

Walailak Journal of Science and Technology Pub Date : 2016-10-10 DOI: 10.14456/VOL14ISS11PP%P

Yurachat Yodyotee, Peerapat Roongsattham, C. Nualsri, U. Meesawat

引用次数: 3

Electrical Characterization by Impedance Spectroscopy of Double Perovskites of Y2NiMnO6 Ceramics 双钙钛矿Y2NiMnO6陶瓷的阻抗谱表征

Walailak Journal of Science and Technology Pub Date : 2016-04-11 DOI: 10.14456/VOL13ISS12PP%P

T. Sareein, N. Albutt

引用次数: 2

Characterization and Secretive Expression in Bacillus subtilis of Endoglucanase from Bacillus safensis Isolated from Freshwater Swamp Forest 淡水沼泽森林沙芽孢杆菌内切葡聚糖酶的鉴定及其在枯草芽孢杆菌中的隐秘表达

Walailak Journal of Science and Technology Pub Date : 2016-03-05 DOI: 10.14456/VOL14ISS5PP%P

P. Kanchanadumkerng, M. Sakka, K. Sakka, C. Wiwat

{"title":"Characterization and Secretive Expression in Bacillus subtilis of Endoglucanase from Bacillus safensis Isolated from Freshwater Swamp Forest","authors":"P. Kanchanadumkerng, M. Sakka, K. Sakka, C. Wiwat","doi":"10.14456/VOL14ISS5PP%P","DOIUrl":"https://doi.org/10.14456/VOL14ISS5PP%P","url":null,"abstract":"Bacillus safensis M3 was newly isolated from freshwater swamp forest soil in western Thailand. The endoglucanase gene of B. safensis M3, cel9A, had an open reading frame of 1,848 bp encoding a 616 amino acid protein. Initial expression in Escherichia coli yielded a low amount of soluble protein in the cytosolic and secreted fractions. Cel9A was successfully expressed by recombinant B. subtilis with a 4-fold greater total enzyme activity than from recombinant E. coli . By SDS-PAGE analysis, the molecular weight of Cel9A was estimated to be 70 kDa. The optimal temperature of Cel9A was 55 °C and the optimal pH was 5 - 8. Cel9A had the highest activity in the pH range from 5 - 8, and the highest stability in pH range 4 to 10, which is useful for industrial applications. Notably, Cel9A was able to hydrolyze both mixed linkage glucan (lichenan) and hemicellulose (konjac glucomannan and oat spelt xylan) better than carboxymethylcellulose. Cel9A also showed a tolerance to metal ions and surfactants. In addition, recombinant B. subtilis with endoglucanase activity has potential for biotechnological applications and benefits in the optimization of large scale enzyme production with minimal medium using agriculture wastes and other inexpensive feedstock materials.","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"14 1","pages":"199-213"},"PeriodicalIF":0.0,"publicationDate":"2016-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66680949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

The Development of Structural Equation Model of Critical Thinking among Nursing Students 护生批判性思维结构方程模型的发展

Walailak Journal of Science and Technology Pub Date : 2016-02-08 DOI: 10.14456/VOL14ISS1PP%P

Kanjana Srisawad, Thanin Ratanaolarn, Krissana Kiddee

{"title":"The Development of Structural Equation Model of Critical Thinking among Nursing Students","authors":"Kanjana Srisawad, Thanin Ratanaolarn, Krissana Kiddee","doi":"10.14456/VOL14ISS1PP%P","DOIUrl":"https://doi.org/10.14456/VOL14ISS1PP%P","url":null,"abstract":"These were to study the critical thinking of nursing students, and to develop and check the goodness of fit of the Structural Equation Model of critical thinking among nursing students, which was compared with an empirical data set. There were 549 first year nursing students. Multi-stage random sampling was used in this research, by cluster random sampling from 20 private higher education institutions that provide nursing courses, comprising 25 percent, and random sampling 5 private higher education institutions, in order to gain 549 samples for this study. These were critical thinking questionnaires and variable effects of critical thinking questionnaires. The data was analyzed by descriptive statistics and the Structural Equation Model. This research indicated that the goodness of fit test of the Structural Equation Model of critical thinking among nursing students was applicable. The results showed the following statistical values: Chi-square = 284.895, df = 248, p-value = 0.054, RMSEA = 0.016, RMR = 0.009, GFI = 0.968, CFI = 0.997 and c 2 /df = 1.149; this indicated that the Structural Equation Model goodness of fit matched the empirical data quite well. The Self Efficiency, Emotional Intelligence, and Learning Style variables had positively direct effects on critical thinking ( b = 0.371, 0.370 and 0.376). The Internal Locus of Control had a negatively direct effect on critical thinking ( b = -0.120). In conclusion, in theoretical and practical teaching, nursing academies should promote critical thinking among nursing students by using the following variables: self-efficiency, emotional intelligence, learning style, and internal locus of control. This model should be used to develop complementary courses on critical thinking for nursing students.","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"14 1","pages":"65-73"},"PeriodicalIF":0.0,"publicationDate":"2016-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66680942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Relationship between Poor Sleep Quality and Psychological Problems among Undergraduate Students in the Southern Thailand. 泰国南部大学生睡眠质量差与心理问题的关系

Walailak Journal of Science and Technology Pub Date : 2016-01-01

Wipawan C Pensuksan, Somrat Lertmaharit, Vitool Lohsoonthorn, Thanapoom Rattananupong, Thanawan Sonkprasert, Bizu Gelaye, Michelle A Williams

{"title":"Relationship between Poor Sleep Quality and Psychological Problems among Undergraduate Students in the Southern Thailand.","authors":"Wipawan C Pensuksan, Somrat Lertmaharit, Vitool Lohsoonthorn, Thanapoom Rattananupong, Thanawan Sonkprasert, Bizu Gelaye, Michelle A Williams","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We conducted this study to fill the noted gap in the available literature by evaluating the prevalence of poor sleep quality in this understudied population and to explore the relationship between sleep quality and psychological problems among undergraduates in Thailand. This study used a cross-sectional survey. Self-administrated questionnaires included the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale, the Depression, Anxiety and Stress Scale and the Thai General Health Questionnaire. The study group included 1,055 undergraduates, aged 18-25 years. The prevalence of poor sleep quality was 42.4%. Students classified as poor quality sleepers reported significantly more psychological problems, indicating a linear trend of progressively worse global sleep quality associated with greater mood and anxiety symptomatology. Prospective studies that include objective measures of sleep duration and quality are needed to more fully develop focused health promotion strategies for Southeast Asian undergraduates.</p>","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"13 4","pages":"235-242"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853815/pdf/nihms767001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34459638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Silk and Its Mixed Fabric for Functional Properties 丝绸及其混纺织物功能性能的研究

Walailak Journal of Science and Technology Pub Date : 2015-11-19 DOI: 10.14456/VOL13ISS11PP%P

Jeyakodi Moses Jeyaraj, M. Arumugam, Vellingiri Kulandaiappan

引用次数: 5

Performance and Carcass Characteristics of Rabbits Fed Oil Supplemented Diets 油饲粮对家兔生产性能和胴体特性的影响

Walailak Journal of Science and Technology Pub Date : 2015-10-23 DOI: 10.14456/VOL13ISS1PP%P

J. A. Omar, A. Zaza

{"title":"Performance and Carcass Characteristics of Rabbits Fed Oil Supplemented Diets","authors":"J. A. Omar, A. Zaza","doi":"10.14456/VOL13ISS1PP%P","DOIUrl":"https://doi.org/10.14456/VOL13ISS1PP%P","url":null,"abstract":"The objective of this study is to investigate the effects of feeding different dietary fat supplements in the finisher rations of Baladi rabbits, including sesame oil (SO), olive oil sediments (OOS), and poultry grease (PG), in comparison to the traditional oil supplement, the soybean soap stock oil (SS), on growth performance, blood lipid profile, dressing percentage and carcass cut, and meat quality: water holding capacity (WHC) and cell forming unit (CFU). A total of 48 Baladi rabbits were used, with individual body weights (BW) of 519 ± 22 g at the beginning of the experiment. Rabbits were randomly divided into 4 groups of 12, and those in each group were individually fed cereal grain-soybean meal (SBM) with a fixed amount ( i.e . 30 g/kg dry matter (DM)) of oil, being either soybean oil (SOY), olive oil sediments (OOS), recycled restaurant oil (RRO), or poultry grease (PG). All rations were isonitrogenous and contained iso-metabolizable energy (ME). At the end of the 44 day feeding trial, all animals were slaughtered. Rabbits fed a SOY supplemented diet consumed more (P < 0.05) feed than those fed the OOS, RRO, or PG supplemented diets. However, rabbits fed the SOY had a better (P < 0.05) feed conversion ratio than rabbits fed the OOS, PG, or RRO diets. Oil source had no effect on carcass components weights. Liver was heavier (P < 0.05) in rabbits fed the SOY supplemented diet. However, the RRO fed rabbits had heavier (P < 0.05) small intestine, large intestine and cecum. In conclusion, the positive effects of the tested oil supplements ( i.e. RRO, OOS, PG) on the studied performance and carcass traits is encouraging, but more investigation is needed to identify the optimal levels for these supplements in various diets of local rabbits. doi: 10.14456/WJST.2016.10","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"10 1","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"2015-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66680435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genetic Diversity in Slender Walking Catfish (Clarias nieuhofii) Populations: Implications for Population Management 细长行走鲶鱼(Clarias nieuhofii)种群遗传多样性:种群管理意义

Walailak Journal of Science and Technology Pub Date : 2015-10-23 DOI: 10.14456/VOL13ISS6PP%P

Jamjun Pechsiri, A. Vanichanon

{"title":"Genetic Diversity in Slender Walking Catfish (Clarias nieuhofii) Populations: Implications for Population Management","authors":"Jamjun Pechsiri, A. Vanichanon","doi":"10.14456/VOL13ISS6PP%P","DOIUrl":"https://doi.org/10.14456/VOL13ISS6PP%P","url":null,"abstract":"Random amplified polymorphic DNA (RAPD) markers were applied to assess the genetic diversity and population structure of the slender walking catfish ( Clarias nieuhofii , Clariidae) from 3 wild populations in peat swamp forests in southern Thailand (Surat Thani , Narathiwat, and Phatthalung). The selected 14 RAPD primers produced 105 RAPD bands, ranging from 6 to 11 bands per primer, and ranging in size between 400 and 3,000 bp. The percentage of polymorphic loci, gene diversity and Shannon’s information index values were 75.00 %, 0.2252, and 0.3443 for Surat Thani; 86.59 %, 0.2982, and 0.4441 for Narathiwat, and 96.25 %, 0.3371, and 0.5049 for Phatthalung, respectively. Among the 3 populations, the highest genetic distance (0.2213) was found between the Narathiwat and Surat Thani populations. High genetic differentiation ( G st = 0.2815) was detected in 3 populations with low gene flow ( Nm = 1.2762) among the overall populations. The clustering pattern obtained by the unweighted pair-group (UPGMA) method separated the C. nieuhofii samples into 3 groups, but 2 clusters. The results indicated a high level of genetic variation and genetic differentiation among C. nieuhofii from different populations in southern Thailand. This information would be useful to construct appropriate breeding programs, and could help conserve populations used as potential sources for stock management, restocking programs, and sustainable uses.","PeriodicalId":38275,"journal":{"name":"Walailak Journal of Science and Technology","volume":"13 1","pages":"511-519"},"PeriodicalIF":0.0,"publicationDate":"2015-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66680099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1