Cilia最新文献

{"title":"Tetrahymena basal bodies","authors":"Brian A Bayless, D. Galati, C. Pearson","doi":"10.1186/s13630-016-0022-8","DOIUrl":"https://doi.org/10.1186/s13630-016-0022-8","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13630-016-0022-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current topics of functional links between primary cilia and cell cycle","authors":"I. Izawa, H. Goto, Kousuke Kasahara, M. Inagaki","doi":"10.1186/s13630-015-0021-1","DOIUrl":"https://doi.org/10.1186/s13630-015-0021-1","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13630-015-0021-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture and detection of primary cilia in endothelial cell models.","authors":"Yi Chung Lim,&nbsp;Sue R McGlashan,&nbsp;Michael T Cooling,&nbsp;David S Long","doi":"10.1186/s13630-015-0020-2","DOIUrl":"https://doi.org/10.1186/s13630-015-0020-2","url":null,"abstract":"<p><strong>Background: </strong>The primary cilium is a sensor of blood-induced forces in endothelial cells (ECs). Studies that have examined EC primary cilia have reported a wide range of cilia incidence (percentage of ciliated cells). We hypothesise that this variation is due to the diversity in culture conditions in which the cells are grown. We studied two EC types: human umbilical vein endothelial cells (HUVECs) and human microvascular endothelial cells (HMEC-1s). Both cell types were grown in media containing foetal bovine serum (FBS) at high (20 % FBS and 10 % FBS for HUVECs and HMEC-1s, respectively) or low (2 % FBS) concentrations. Cells were then either fixed at confluence, serum-starved or grown post-confluence for 5 days in corresponding expansion media (cobblestone treatment). For each culture condition, we quantified cilia incidence and length.</p><p><strong>Results: </strong>HUVEC ciliogenesis is dependent on serum concentration during the growth phase; low serum (2 % FBS) HUVECs were not ciliated, whereas high serum (20 % FBS) confluent HUVECs have a cilia incidence of 2.1 ± 2.2 % (median ± interquartile range). We report, for the first time, the presence of cilia in the HMEC-1 cell type. HMEC-1s have between 2.2 and 3.5 times greater cilia incidence than HUVECs (p < 0.001). HMEC-1s also have shorter cilia compared to HUVECs (3.0 ± 1.0 μm versus 5.1 ± 2.4 μm, at confluence, p = 0.003).</p><p><strong>Conclusions: </strong>We demonstrate that FBS plays a role in determining the prevalence of cilia in HUVECs. In doing so, we highlight the importance of considering a commonly varied parameter (% FBS), in the experimental design. We recommend that future studies examining large blood vessel EC primary cilia use confluent HUVECs grown in high serum medium, as we found these cells to have a higher cilia incidence than low serum media HUVECs. For studies interested in microvasculature EC primary cilia, we recommend using cobblestone HMEC-1s grown in high serum medium, as these cells have a 19.5 ± 6.2 % cilia incidence.</p>","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 ","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2015-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13630-015-0020-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34056063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.","authors":"Noëlle Cognard,&nbsp;Maria J Scerbo,&nbsp;Cathy Obringer,&nbsp;Xiangxiang Yu,&nbsp;Fanny Costa,&nbsp;Elodie Haser,&nbsp;Dane Le,&nbsp;Corinne Stoetzel,&nbsp;Michel J Roux,&nbsp;Bruno Moulin,&nbsp;Hélène Dollfus,&nbsp;Vincent Marion","doi":"10.1186/s13630-015-0019-8","DOIUrl":"https://doi.org/10.1186/s13630-015-0019-8","url":null,"abstract":"<p><strong>Background: </strong>Bardet-Biedl Syndrome (BBS) is a genetically heterogeneous ciliopathy with clinical cardinal features including retinal degeneration, obesity and renal dysfunction. To date, 20 BBS genes have been identified with BBS10 being a major BBS gene found to be mutated in almost 20 percent of all BBS patients worldwide. It codes for the BBS10 protein which forms part of a chaperone complex localized at the basal body of the primary cilium. Renal dysfunction in BBS patients is one of the major causes of morbidity in human patients and is associated initially with urinary concentration defects related to water reabsorption impairment in renal epithelial cells. The aim of this study was to study and compare the impact of a total Bbs10 inactivation (Bbs10 (-/-)) with that of a specific renal epithelial cells inactivation (Bbs10  (fl/fl) ; Cdh16-Cre (+/-)).</p><p><strong>Results: </strong>We generated the Bbs10 (-/-) and Bbs10  (fl/fl) ; Cadh16-Cre (+/-) mouse model and characterized them. Bbs10 (-/-) mice developed obesity, retinal degeneration, structural defects in the glomeruli, polyuria associated with high circulating arginine vasopressin (AVP) concentrations, and vacuolated, yet ciliated, renal epithelial cells. On the other hand, the Bbs10  (fl/fl) ; Cadh16-Cre (+/-)mice displayed no detectable impairment.</p><p><strong>Conclusions: </strong>These data highlight the importance of a systemic Bbs10 inactivation to trigger averted renal dysfunction whereas a targeted absence of BBS10 in the renal epithelium is seemingly non-deleterious.</p>","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 ","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2015-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s13630-015-0019-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34091141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-throughput genome-wide siRNA screen for ciliogenesis identifies new ciliary functional components and ciliopathy genes","authors":"K. Szymanska, G. Wheway, D. Doherty, M. Schmidts, D. Mans, Tmt Nguyen, K. Boldt, G. Tödt, Z. Abdelhamed, K. Wunderlich, S. Natarajan, D. Parry, C. Logan, W. Herridge, N. Sorusch, U. Wolfrum, M. Ueffing, R. Roepman, H. Mitchison, C. Johnson","doi":"10.1186/2046-2530-4-S1-O12","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-O12","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"O12 - O12"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-O12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65815883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crumbs proteins control ciliogenesis and centrosome organization: what about the mechanism?","authors":"M. Barthélémy-Requin, JP Chauvin, CL Baron-Gaillard, P. Rashbass, D. Massey‐Harroche, A. Bivic","doi":"10.1186/2046-2530-4-S1-P23","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-P23","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"P23 - P23"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-P23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65816993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rescue of corpus callosum agenesis in two ciliary mutants by the short repressor isoform of Gli3","authors":"C. Laclef, I. Anselme, L. Besse, M. Catala, D. Baas, M. Paschaki, B. Durand, S. Schneider-Maunoury","doi":"10.1186/2046-2530-4-S1-P36","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-P36","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"P36 - P36"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-P36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65818056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-box promoter motif searches: from C. elegans to humans to novel candidate ciliopathies","authors":"G. Lauter, K. Tammimies, A. Bieder, R. Torchet, H. Matsson, M. Peyrard, J. Burghoorn, E. Castrén, J. Kere, I. Tapia-Páez, P. Swoboda","doi":"10.1186/2046-2530-4-S1-P56","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-P56","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"P56 - P56"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-P56","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65818636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of upper airway ciliary beat by coupling isolated and collective cilia motion analysis","authors":"M. Bottier, S. Blanchon, M. Filoche, D. Isabey, A. Coste, E. Escudier, J. Papon, Louis","doi":"10.1186/2046-2530-4-S1-P86","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-P86","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"P86 - P86"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-P86","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65820705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systems biology approach towards the prediction of ciliopathy mechanisms","authors":"K. Koutroumpas, J. V. Dam, G. Toedt, Q. Lu, J. V. Reeuwijk, K. Boldt, T. Gibson, R. Roepman, M. Ueffing, RB Russell, M. Huynen, M. Elati, F. Képès","doi":"10.1186/2046-2530-4-S1-P88","DOIUrl":"https://doi.org/10.1186/2046-2530-4-S1-P88","url":null,"abstract":"","PeriodicalId":38134,"journal":{"name":"Cilia","volume":"4 1","pages":"P88 - P88"},"PeriodicalIF":0.0,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2046-2530-4-S1-P88","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65820991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}