Biomolecular Detection and Quantification最新文献

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How to make Mathematics Biology's next and better microscope 如何使数学生物学成为下一个更好的显微镜
Biomolecular Detection and Quantification Pub Date : 2014-09-01 DOI: 10.1016/j.bdq.2014.09.001
Jim Huggett, Justin O’Grady, Stephen Bustin
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引用次数: 4
Determining lower limits of detection of digital PCR assays for cancer-related gene mutations 确定数字PCR检测癌症相关基因突变的下限
Biomolecular Detection and Quantification Pub Date : 2014-09-01 DOI: 10.1016/j.bdq.2014.08.001
Coren A. Milbury , Qun Zhong , Jesse Lin, Miguel Williams, Jeff Olson, Darren R. Link, Brian Hutchison
{"title":"Determining lower limits of detection of digital PCR assays for cancer-related gene mutations","authors":"Coren A. Milbury ,&nbsp;Qun Zhong ,&nbsp;Jesse Lin,&nbsp;Miguel Williams,&nbsp;Jeff Olson,&nbsp;Darren R. Link,&nbsp;Brian Hutchison","doi":"10.1016/j.bdq.2014.08.001","DOIUrl":"10.1016/j.bdq.2014.08.001","url":null,"abstract":"<div><p>Digital PCR offers very high sensitivity compared to many other technologies for processing molecular detection assays. Herein, a process is outlined for determining the lower limit of detection (LoD) of two droplet-based digital PCR assays for point mutations of the epidermal growth factor receptor (<em>EGFR</em>) gene. Hydrolysis probe mutation-detection assays for <em>EGFR</em> p.L858R and p.T790M mutations were characterized in detail. Furthermore, sixteen additional cancer-related mutation assays were explored by the same approach. For the <em>EGFR</em> L8585R assay, the assay sensitivity is extremely good, and thus, the LoD is limited by the amount of amplifiable DNA that is analyzed. With 95% confidence limits, the LoD is one mutant in 180,000 wild-type molecules for the evaluation of 3.3<!--> <!-->μg of genomic DNA, and detection of one mutant molecule in over 4 million wild-type molecules was achieved when 70 million copies of DNA were processed. The measured false-positive rate for the <em>EGFR</em> L8585R assay is one in 14 million, which indicates the theoretical LoD if an unlimited amount of DNA is evaluated. For the <em>EFGR</em> T790M assay, the LoD is one mutant in 13,000 for analysis of a 3.3<!--> <!-->μg sample of genomic DNA, and the dPCR assay limit sensitivity approaches one mutant in 22,000 wild-type molecules.</p></div>","PeriodicalId":38073,"journal":{"name":"Biomolecular Detection and Quantification","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bdq.2014.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54133400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 146
Aims & Scope 目标及范围
Biomolecular Detection and Quantification Pub Date : 2014-09-01 DOI: 10.1016/S2214-7535(14)00007-2
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引用次数: 0
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