S. Mandel, S. Menon, W. Harshawardhan, Q. Su, R. Grobe
{"title":"Numerical solution techniques to the time-dependent Maxwell for highly scattering media","authors":"S. Mandel, S. Menon, W. Harshawardhan, Q. Su, R. Grobe","doi":"10.1364/ecbo.2001.4431_165","DOIUrl":"https://doi.org/10.1364/ecbo.2001.4431_165","url":null,"abstract":"We discuss how a spectral-domain method in combination with a split-operator technique can be used to calculate exact solutions of the time-dependent Maxwell We apply this technique to study the propagation of a light pulse through an inhomogeneous medium consisting of practically arbitrarily shaped dielectric and metallic materials.","PeriodicalId":377673,"journal":{"name":"Photon Migration, Optical Coherence Tomography, and Microscopy","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126737731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Webb, D. Elson, Jesse E. Siegel, S. Lévêque-Fort, Y. Gu, D. Parsons-Karavassilis, M. Cole, P. French, M. Lever, L. O. Sueharov, M. Neil, R. Juškaitis, T. Wilson
{"title":"‘5-D’ fluorescence microscopy","authors":"S. Webb, D. Elson, Jesse E. Siegel, S. Lévêque-Fort, Y. Gu, D. Parsons-Karavassilis, M. Cole, P. French, M. Lever, L. O. Sueharov, M. Neil, R. Juškaitis, T. Wilson","doi":"10.1364/ecbo.2001.4431_87","DOIUrl":"https://doi.org/10.1364/ecbo.2001.4431_87","url":null,"abstract":"We report a whole-field fluorescence imaging microscope that combines 3-D spatial resolution by optical sectioning, using structured illumination, with fluorescence lifetime imaging and spectrally-resolved imaging. We show the potential of this technique in the elimination of common artefacts in fluorescence lifetime imaging and apply it to study the dependence of the lifetime on the emission wavelength in biological tissue.","PeriodicalId":377673,"journal":{"name":"Photon Migration, Optical Coherence Tomography, and Microscopy","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131614304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-Photon Fluorescence Imaging through Biological Tissue and Image Reconstruction","authors":"X. Gan, M. Gu","doi":"10.1364/ecbo.2001.4431_226","DOIUrl":"https://doi.org/10.1364/ecbo.2001.4431_226","url":null,"abstract":"In this paper, image formation under single-photon (1-p), two-photon (2-p) and three-photon (3-p) fluorescence imaging through turbid media which consist of different sized scatterers has been investigated in detail. It has been demonstrated that the size of scattering particles plays an important role in determining whether to use 1-p, 2-p, or 3-p excitation. For small scatterers, where Rayleigh scattering is dominant, multi-photon excitation provides significantly better resolution. Such improvement reduces dramatically for large scatterers, where Mie scattering becomes dominant. Another disadvantage of using multi-photon fluorescence excitation in highly scattered media is that penetration depth is limited by fast dropping of signal strength in deep tissue imaging. In this paper, we introduce a deconvolution method with a novel concept of the effective point spread function, which is effective in restoring the loss of imaging resolution caused by multiple scattering in a tissue medium.","PeriodicalId":377673,"journal":{"name":"Photon Migration, Optical Coherence Tomography, and Microscopy","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131452155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction for in-homogeneously distributed absorbers in spatially resolved diffuse reflectance spectroscopy","authors":"R. V. van Veen, H. Sterenborg","doi":"10.1364/ecbo.2001.4431_192","DOIUrl":"https://doi.org/10.1364/ecbo.2001.4431_192","url":null,"abstract":"Absorption and reduced scattering coefficients calculated from spatially resolved diffuse reflectance measurements are usually assumed to be unreliable for wavelengths lower than approximately 600 nm. A correction factor was developed for non-homogenous distribution of absorbers concentrated in discrete cylindrical blood vessels. The concept of an effective absorption coefficient extends the applicability of diffusion theory too much lower wavelength regions.","PeriodicalId":377673,"journal":{"name":"Photon Migration, Optical Coherence Tomography, and Microscopy","volume":"2006 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124989363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Radhakrishnan, D. Reeves, J. Lass, D. Bardenstein, A. Rollins, V. Westphal, Jonathan E. Roth, J. Izatt
{"title":"Non-invasive characterization of anterior segment structures using real-time optical coherence tomography at 1310nm","authors":"S. Radhakrishnan, D. Reeves, J. Lass, D. Bardenstein, A. Rollins, V. Westphal, Jonathan E. Roth, J. Izatt","doi":"10.1364/ecbo.2001.4431_43","DOIUrl":"https://doi.org/10.1364/ecbo.2001.4431_43","url":null,"abstract":"Real-time optical coherence tomography (OCT) was used to visualize and quantify structures in the anterior segment of the eye. Current results of ongoing clinical trials are presented. Preliminary data indicates strong potential for the use of real time OCT as a tool for noninvasive characterization of the anterior chamber angle and for anterior segment biometry.","PeriodicalId":377673,"journal":{"name":"Photon Migration, Optical Coherence Tomography, and Microscopy","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129364591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}