S. Webb, D. Elson, Jesse E. Siegel, S. Lévêque-Fort, Y. Gu, D. Parsons-Karavassilis, M. Cole, P. French, M. Lever, L. O. Sueharov, M. Neil, R. Juškaitis, T. Wilson
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We report a whole-field fluorescence imaging microscope that combines 3-D spatial resolution by optical sectioning, using structured illumination, with fluorescence lifetime imaging and spectrally-resolved imaging. We show the potential of this technique in the elimination of common artefacts in fluorescence lifetime imaging and apply it to study the dependence of the lifetime on the emission wavelength in biological tissue.
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