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Journal of Integrated OMICS最新文献

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Ultrasound-Assisted Extraction- and Liquid Chromatography-Based Method Development and Validation for Obtaining and Qualitative Determination of Apple Pomace Three Triterpene Acids using Analytical Quality by Design 基于超声辅助萃取和液相色谱法的苹果渣三萜酸定性检测方法的开发与验证--采用 "质量源于设计 "的分析方法
Journal of Integrated OMICS Pub Date : 2024-06-12 DOI: 10.5584/jiomics.v14i2.232
I. Rubashvili, Mzia Tsitsagi, M. Chkhaidze, K. Ebralidze
{"title":"Ultrasound-Assisted Extraction- and Liquid Chromatography-Based Method Development and Validation for Obtaining and Qualitative Determination of Apple Pomace Three Triterpene Acids using Analytical Quality by Design","authors":"I. Rubashvili, Mzia Tsitsagi, M. Chkhaidze, K. Ebralidze","doi":"10.5584/jiomics.v14i2.232","DOIUrl":"https://doi.org/10.5584/jiomics.v14i2.232","url":null,"abstract":"Background: Apple pomace has garnered significant attention within the life sciences domain due to its underutilized status as a waste material from apple processing. It represents a cost-effective and abundant source of triterpene acids due to its multifunctional clinical, nutritional, and pharmaceutical benefits. \u0000Purpose: The present study aimed to develop and validate a new, selective, effective, robust and reproducible laboratory methodology based on extraction, purification and analytical procedures to obtain and determining three major triterpene acids – Ursolic acid (UA), Oleanolic acid (OA) and Betulinic acid (BA) into the dry extracted product from apple pomace. \u0000Method: A new, cost-efficient, rapid, selective and high-yield two-stage ultrasound-assisted extraction procedure was developed and the effect of critical parameters: ultrasonic power, extraction time, solvent volume, temperature, and the amount of raw material on the extraction process were investigated. The dry column vacuum chromatography technique was used for purification to remove unwanted non-polar and polar impurities from the target bioactive compounds; A new, effective, specific, sensitive, and rapid HPLC analytical procedure was developed using analytical quality by design (AQbD) approach and validated according to ICH guidelines. \u0000Conclusion: The method has a good accuracy (the mean recovery >95 %) and linearity (R2>0.999).  The limit of quantitation (LOQ) is 0.0001 mg/mL for UA, 0.00005 mg/mL for OA and 0.000025 mg/mL for BA.  The validation results confirm that the method is specific, precise and robust. The purity of the extracted and purified target product from apple pomace is not less than 93 %. The developed laboratory methodology is capable of being considered for industrial purposes and through the appropriate technology transfer process can be successfully transferred to the industrial scale.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141350932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vol 14 NO. 2 (2024) 第 14 卷第 2 期（2024 年）
Journal of Integrated OMICS Pub Date : 2024-06-12 DOI: 10.5584/jiomics.v14i2.235
José Luís Capelo Martínez
{"title":"Vol 14 NO. 2 (2024)","authors":"José Luís Capelo Martínez","doi":"10.5584/jiomics.v14i2.235","DOIUrl":"https://doi.org/10.5584/jiomics.v14i2.235","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vol. 14 No. 1 (2024) 第 14 卷第 1 期（2024 年）
Journal of Integrated OMICS Pub Date : 2024-04-15 DOI: 10.5584/jiomics.v14i1.230
J. Capelo
{"title":"Vol. 14 No. 1 (2024)","authors":"J. Capelo","doi":"10.5584/jiomics.v14i1.230","DOIUrl":"https://doi.org/10.5584/jiomics.v14i1.230","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
DTT protein equalization and Tryptophan protein quantification as a powerful tool in analytical proteomics. DTT 蛋白平衡和色氨酸蛋白定量是分析蛋白质组学的有力工具。
Journal of Integrated OMICS Pub Date : 2024-04-15 DOI: 10.5584/jiomics.v14i1.229
Inês F. Domingos, Luís B. Carvalho, J. Capelo, C. Lodeiro, Hugo M. Santos
{"title":"DTT protein equalization and Tryptophan protein quantification as a powerful tool in analytical proteomics.","authors":"Inês F. Domingos, Luís B. Carvalho, J. Capelo, C. Lodeiro, Hugo M. Santos","doi":"10.5584/jiomics.v14i1.229","DOIUrl":"https://doi.org/10.5584/jiomics.v14i1.229","url":null,"abstract":"Assessing total protein levels in biological samples is a common procedure in biochemistry and molecular biology. In this study, we compare tryptophan fluorescence (WF) with Bradford and BCA assays to determine total protein in serum samples. Our results indicate that tryptophan fluorescence spectrometry is an efficient, sensitive, and straightforward technique for quantifying proteins in serum. We observed minimal variation between the three methods: BCA de one with the lowers LOD and LOQ. The tryptophan method offers the possibility of reusing the intact sample that does not need colourimetric reagents for quantification. Consequently, free tryptophan serves as a reliable universal standard. This assay can be performed using a conventional fluorescence spectrometer with cuvettes or in a 96-well plate format with a plate reader. The method was successfully used as proof of concept, using serum from patients diagnosed with myeloma and serum from healthy donors.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Navigating the molecular diversity of SARS-CoV-2: early pandemic insights from comparative phylogenetic analysis 探索 SARS-CoV-2 的分子多样性：从比较系统发育分析中洞察早期大流行的原因
Journal of Integrated OMICS Pub Date : 2024-04-15 DOI: 10.5584/jiomics.v14i1.228
Chahinez Triqui, Ilyes Zatla, Wafaa Lemerini, Nora Benmadani, Mohammed Charaf Eddine Houari, S. Gaouar, N. Soulimane
{"title":"Navigating the molecular diversity of SARS-CoV-2: early pandemic insights from comparative phylogenetic analysis","authors":"Chahinez Triqui, Ilyes Zatla, Wafaa Lemerini, Nora Benmadani, Mohammed Charaf Eddine Houari, S. Gaouar, N. Soulimane","doi":"10.5584/jiomics.v14i1.228","DOIUrl":"https://doi.org/10.5584/jiomics.v14i1.228","url":null,"abstract":"The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in December 2019 precipitated the onset of the COVID-19 pandemic, which swiftly spread across more than 214 countries and territories, posing a significant global health crisis. In response, laboratories worldwide have embarked on extensive efforts to characterize the genomic landscape of the virus, employing a myriad of sophisticated genomic analysis techniques. This study endeavors to undertake a comprehensive exploration into the genetic diversity, geographical distribution, and virulence determinants of SARS-CoV-2 clades across 11 diverse countries, employing advanced computational biology methodologies. Leveraging molecular data sourced from prominent international databases, the analysis aims to unravel the intricate phylogenetic relationships and mutational dynamics exhibited by various viral strains circulating worldwide. The findings of this investigation promise to yield invaluable insights into the evolutionary trajectory of SARS-CoV-2, shedding light on potential therapeutic targets and informing strategies for mitigating the impact of the ongoing pandemic on global public health. Results highlight significant genetic diversity among SARS-CoV-2 strains across different countries, with phylogenetic analysis revealing distinct subclass groupings within each country. A manual comparison of sequences identified numerous mutations, with certain mutations associated with increased virulence. Comparison of clade G and clade O sequences revealed differences in mutation profiles, suggesting potential links to virulence and transmissibility. These findings underscore the dynamic nature of SARS-CoV-2 evolution and the importance of monitoring genetic changes for public health interventions.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vol 13 NO. 2 (2023) 第 13 卷第 2 期（2023 年）
Journal of Integrated OMICS Pub Date : 2023-12-30 DOI: 10.5584/jiomics.v13i2.225
J. Capelo
{"title":"Vol 13 NO. 2 (2023)","authors":"J. Capelo","doi":"10.5584/jiomics.v13i2.225","DOIUrl":"https://doi.org/10.5584/jiomics.v13i2.225","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139141511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibiotic resistance and the one health approach. The importance of teaching OMICS at Universities. 抗生素耐药性与统一健康方法。OMICS 教学在大学中的重要性。
Journal of Integrated OMICS Pub Date : 2023-12-30 DOI: 10.5584/jiomics.v13i2.224
Gilberto Igrejas, J. Capelo, Pedro Campos, Hugo Santos, C. Lodeiro, Luis Carvalho, E. Oliveira, Patricia Alexandra Curado Quintas Diniz Poeta
{"title":"Antibiotic resistance and the one health approach. The importance of teaching OMICS at Universities.","authors":"Gilberto Igrejas, J. Capelo, Pedro Campos, Hugo Santos, C. Lodeiro, Luis Carvalho, E. Oliveira, Patricia Alexandra Curado Quintas Diniz Poeta","doi":"10.5584/jiomics.v13i2.224","DOIUrl":"https://doi.org/10.5584/jiomics.v13i2.224","url":null,"abstract":"Letter to the editor","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139140678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vol 13 NO. 1 (2023) 第十三卷第一号1 (2023)
Journal of Integrated OMICS Pub Date : 2023-07-31 DOI: 10.5584/jiomics.v13i1.222
José Luis Capelo
{"title":"Vol 13 NO. 1 (2023)","authors":"José Luis Capelo","doi":"10.5584/jiomics.v13i1.222","DOIUrl":"https://doi.org/10.5584/jiomics.v13i1.222","url":null,"abstract":"","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135358104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
INSIGHTS INTO THE USE OF MICROSAMPLING FOR OMICS STUDIE 微采样在组学研究中的应用
Journal of Integrated OMICS Pub Date : 2023-07-31 DOI: 10.5584/jiomics.v13i1.221
Laura Mercolini, Michele Protti, Luis B. Carvalho, Pedro A.D. Teigas-Campos, Sarah Palano, Elisa Milandri, Claudia Volpi, Carlos Lodeiro, Hugo M. Santos, José L. Capelo, Roberto Mandrioli
{"title":"INSIGHTS INTO THE USE OF MICROSAMPLING FOR OMICS STUDIE","authors":"Laura Mercolini, Michele Protti, Luis B. Carvalho, Pedro A.D. Teigas-Campos, Sarah Palano, Elisa Milandri, Claudia Volpi, Carlos Lodeiro, Hugo M. Santos, José L. Capelo, Roberto Mandrioli","doi":"10.5584/jiomics.v13i1.221","DOIUrl":"https://doi.org/10.5584/jiomics.v13i1.221","url":null,"abstract":"No abstract needed for Letters to the Editor.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135358103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Computational Nanomedicine: Helping Us Get Through COVID-19 计算纳米医学:帮助我们度过COVID-19
Journal of Integrated OMICS Pub Date : 2022-12-31 DOI: 10.5584/jiomics.v12i2.213
Thomas Webster
{"title":"Computational Nanomedicine: Helping Us Get Through COVID-19","authors":"Thomas Webster","doi":"10.5584/jiomics.v12i2.213","DOIUrl":"https://doi.org/10.5584/jiomics.v12i2.213","url":null,"abstract":"This is a letter to the editor so there is no astract.","PeriodicalId":37675,"journal":{"name":"Journal of Integrated OMICS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44486611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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