Genetics Research International最新文献_第2页

Electronic Northern Analysis of Genes and Modeling of Gene Networks Underlying Bovine Milk Fat Production. 牛乳脂生产基因的电子北方分析和基因网络建模。

Genetics Research International Pub Date : 2017-01-01 Epub Date: 2017-11-07 DOI: 10.1155/2017/1910530

Bhaskar Ganguly, Tanuj Kumar Ambwani, Sunil Kumar Rastogi

{"title":"Electronic Northern Analysis of Genes and Modeling of Gene Networks Underlying Bovine Milk Fat Production.","authors":"Bhaskar Ganguly, Tanuj Kumar Ambwani, Sunil Kumar Rastogi","doi":"10.1155/2017/1910530","DOIUrl":"https://doi.org/10.1155/2017/1910530","url":null,"abstract":"Milk fat is one of the most important economic traits in dairy animals. Yet, the biological machinery involved in milk fat synthesis remains poorly understood. In the present study, expression profiling of 45 genes involved in lipid biosynthesis and secretion was performed using a computational approach to identify those genes that are differentially expressed in mammary tissue. Transcript abundance was observed for genes associated with nine bioprocesses, namely, fatty acid import into cells, xenobiotic and cholesterol transport, acetate and fatty acid activation and intracellular transport, fatty acid synthesis and desaturation, triacylglycerol synthesis, sphingolipid synthesis, lipid droplet formation, ketone body utilization, and regulation of transcription in mammary, skin, and muscle tissue. Relative expression coefficient of the genes was derived based on the transcript abundance across the three tissue types to determine the genes that were preferentially expressed during lactation. 13 genes (ACSS1, ACSS2, ADFP, CD36, FABP3, FASN, GPAM, INSIG1, LPL, SCD5, SPTLC1, SREBF1, and XDH) showed higher expression in the mammary tissue of which 6 (ADFP, FASN, GPAM, LPL, SREBF1, and XDH) showed higher expression during adulthood. Further, interaction networks were mapped for these genes to determine the nature of interactions and to identify the major genes in the milk fat biosynthesis and secretion pathways.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2017 ","pages":"1910530"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1910530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35664810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Frequency of c.35delG Mutation in GJB2 Gene (Connexin 26) in Syrian Patients with Nonsyndromic Hearing Impairment. 叙利亚非综合征性听力障碍患者GJB2基因(连接蛋白26)c.35delG突变频率

Genetics Research International Pub Date : 2017-01-01 Epub Date: 2017-12-06 DOI: 10.1155/2017/5836525

Hazem Kaheel, Andreas Breß, Mohamed A Hassan, Aftab Ali Shah, Mutaz Amin, Yousuf H Y Bakhit, Marlies Kniper

{"title":"Frequency of c.35delG Mutation in GJB2 Gene (Connexin 26) in Syrian Patients with Nonsyndromic Hearing Impairment.","authors":"Hazem Kaheel, Andreas Breß, Mohamed A Hassan, Aftab Ali Shah, Mutaz Amin, Yousuf H Y Bakhit, Marlies Kniper","doi":"10.1155/2017/5836525","DOIUrl":"https://doi.org/10.1155/2017/5836525","url":null,"abstract":"Background: Hearing impairments (HI) are the most common birth defect worldwide. Very large numbers of genes have been identified but the most profound is GJB2. The clinical interest regarding this gene is very pronounced due to its high carrier frequency (0.5-5.4%) across different ethnic groups. This study aimed to determine the prevalence of common GJB2 mutations in Syrian patients with profound sensorineural HI.Methods: We carried out PCR, restriction enzyme based screening, and sequencing of 132 Syrian patients diagnosed clinically with hereditary deafness for different GJB2 mutations.Results: The result revealed that, in GJB2 gene, c.35delG is the most prevalent among affected studied subjects (13.64%), followed by c.457G>A (2.4%).Conclusion: The benefit of this study on the one hand is its first report of prelingual deafness causative gene mutations identified by sequencing technology in the Syrian families. It is obvious from the results that the deployment in biomedical research is highly effective and has a great impact on the ability to uncover the cause of genetic variation in different genetic diseases.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2017 ","pages":"5836525"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/5836525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35762443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The Noncell Autonomous Requirement of Proboscipedia for Growth and Differentiation of the Distal Maxillary Palp during Metamorphosis of Drosophila melanogaster. 黑腹果蝇变态过程中上颌远端触须生长和分化对鼻足的非细胞自主要求。

Genetics Research International Pub Date : 2017-01-01 Epub Date: 2017-03-05 DOI: 10.1155/2017/2624170

Anthony Percival-Smith, Gabriel Ponce, Jacob J H Pelling

{"title":"The Noncell Autonomous Requirement of Proboscipedia for Growth and Differentiation of the Distal Maxillary Palp during Metamorphosis of Drosophila melanogaster.","authors":"Anthony Percival-Smith, Gabriel Ponce, Jacob J H Pelling","doi":"10.1155/2017/2624170","DOIUrl":"https://doi.org/10.1155/2017/2624170","url":null,"abstract":"The Drosophila maxillary palpus that develops during metamorphosis is composed of two elements: the proximal maxillary socket and distal maxillary palp. The HOX protein, Proboscipedia (PB), was required for development of the proximal maxillary socket and distal maxillary palp. For growth and differentiation of the distal maxillary palp, PB was required in the cells of, or close to, the maxillary socket, as well as the cells of the distal maxillary palp. Therefore, PB is required in cells outside the distal maxillary palp for the expression, by some mechanism, of a growth factor or factors that promote the growth of the distal maxillary palp. Both wingless (wg) and hedgehog (hh) genes were expressed in cells outside the distal maxillary palp in the lancinia and maxillary socket, respectively. Both wg and hh were required for distal maxillary palp growth, and hh was required noncell autonomously for distal maxillary palp growth. However, expression of wg-GAL4 and hh-GAL4 during maxillary palp differentiation did not require PB, ruling out a direct role for PB in the regulation of transcription of these growth factors.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2017 ","pages":"2624170"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/2624170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34868259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Procaine Induces Epigenetic Changes in HCT116 Colon Cancer Cells 普鲁卡因诱导HCT116结肠癌细胞的表观遗传变化

Genetics Research International Pub Date : 2016-10-24 DOI: 10.1155/2016/8348450

H. Sabit, Mariam B Samy, Osama A. M. Said, M. El-Zawahri

{"title":"Procaine Induces Epigenetic Changes in HCT116 Colon Cancer Cells","authors":"H. Sabit, Mariam B Samy, Osama A. M. Said, M. El-Zawahri","doi":"10.1155/2016/8348450","DOIUrl":"https://doi.org/10.1155/2016/8348450","url":null,"abstract":"Colon cancer is the third most commonly diagnosed cancer in the world, and it is the major cause of morbidity and mortality throughout the world. The present study aimed at treating colon cancer cell line (HCT116) with different chemotherapeutic drug/drug combinations (procaine, vorinostat “SAHA,” sodium phenylbutyrate, erlotinib, and carboplatin). Two different final concentrations were applied: 3 μM and 5 μM. Trypan blue test was performed to assess the viability of the cell before and after being treated with the drugs. The data obtained showed that there was a significant decrease in the viability of cells after applying the chemotherapeutic drugs/drug combinations. Also, DNA fragmentation assay was carried out to study the effect of these drugs on the activation of apoptosis-mediated DNA degradation process. The results indicated that all the drugs/drug combinations had a severe effect on inducing DNA fragmentation. Global DNA methylation quantification was performed to identify the role of these drugs individually or in combination in hypo- or hypermethylating the CpG dinucleotide all over the genome of the HCT116 colon cancer cell line. Data obtained indicated that different combinations had different effects in reducing or increasing the level of methylation, which might indicate the effectiveness of combining drugs in treating colon cancer cells.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72689910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Screening for Subtelomeric Rearrangements in Thai Patients with Intellectual Disabilities Using FISH and Review of Literature on Subtelomeric FISH in 15,591 Cases with Intellectual Disabilities 使用FISH筛查泰国智障患者的亚端粒重排，并回顾15591例智障患者亚端粒FISH的文献

Genetics Research International Pub Date : 2016-10-16 DOI: 10.1155/2016/9153740

Chariyawan Charalsawadi, Jariya Khayman, V. Praphanphoj, P. Limprasert

{"title":"Screening for Subtelomeric Rearrangements in Thai Patients with Intellectual Disabilities Using FISH and Review of Literature on Subtelomeric FISH in 15,591 Cases with Intellectual Disabilities","authors":"Chariyawan Charalsawadi, Jariya Khayman, V. Praphanphoj, P. Limprasert","doi":"10.1155/2016/9153740","DOIUrl":"https://doi.org/10.1155/2016/9153740","url":null,"abstract":"We utilized fluorescence in situ hybridization (FISH) to screen for subtelomeric rearrangements in 82 Thai patients with unexplained intellectual disability (ID) and detected subtelomeric rearrangements in 5 patients. Here, we reported on a patient with der(20)t(X;20)(p22.3;q13.3) and a patient with der(3)t(X;3)(p22.3;p26.3). These rearrangements have never been described elsewhere. We also reported on a patient with der(10)t(7;10)(p22.3;q26.3), of which the same rearrangement had been reported in one literature. Well-recognized syndromes were detected in two separated patients, including 4p deletion syndrome and 1p36 deletion syndrome. All patients with subtelomeric rearrangements had both ID and multiple congenital anomalies (MCA) and/or dysmorphic features (DF), except the one with der(20)t(X;20), who had ID alone. By using FISH, the detection rate of subtelomeric rearrangements in patients with both ID and MCA/DF was 8.5%, compared to 2.9% of patients with only ID. Literature review found 28 studies on the detection of subtelomeric rearrangements by FISH in patients with ID. Combining data from these studies and our study, 15,591 patients were examined and 473 patients with subtelomeric rearrangements were determined. The frequency of subtelomeric rearrangements detected by FISH in patients with ID was 3%. Terminal deletions were found in 47.7%, while unbalanced derivative chromosomes were found in 47.9% of the rearrangements.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"81 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89576890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Unique AGG Interruption in the CGG Repeats of the FMR1 Gene Exclusively Found in Asians Linked to a Specific SNP Haplotype 亚洲独有的与特定SNP单倍型相关的FMR1基因CGG重复序列中独特的AGG中断

Genetics Research International Pub Date : 2016-03-02 DOI: 10.1155/2016/8319287

P. Limprasert, J. Thanakitgosate, Kanoot Jaruthamsophon, T. Sripo

{"title":"Unique AGG Interruption in the CGG Repeats of the FMR1 Gene Exclusively Found in Asians Linked to a Specific SNP Haplotype","authors":"P. Limprasert, J. Thanakitgosate, Kanoot Jaruthamsophon, T. Sripo","doi":"10.1155/2016/8319287","DOIUrl":"https://doi.org/10.1155/2016/8319287","url":null,"abstract":"Fragile X syndrome (FXS) is the most common inherited intellectual disability. It is caused by the occurrence of more than 200 pure CGG repeats in the FMR1 gene. Normal individuals have 6–54 CGG repeats with two or more stabilizing AGG interruptions occurring once every 9- or 10-CGG-repeat blocks in various populations. However, the unique (CGG)6AGG pattern, designated as 6A, has been exclusively reported in Asians. To examine the genetic background of AGG interruptions in the CGG repeats of the FMR1 gene, we studied 8 SNPs near the CGG repeats in 176 unrelated Thai males with 19–56 CGG repeats. Of these 176 samples, we identified AGG interruption patterns from 95 samples using direct DNA sequencing. We found that the common CGG repeat groups (29, 30, and 36) were associated with 3 common haplotypes, GCGGATAA (Hap A), TTCATCGC (Hap C), and GCCGTTAA (Hap B), respectively. The configurations of 9A9A9, 10A9A9, and 9A9A6A9 were commonly found in chromosomes with 29, 30, and 36 CGG repeats, respectively. Almost all chromosomes with Hap B (22/23) carried at least one 6A pattern, suggesting that the 6A pattern is linked to Hap B and may have originally occurred in the ancestors of Asian populations.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88338527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Induced Pluripotent Stem Cell as a New Source for Cancer Immunotherapy 诱导多能干细胞作为肿瘤免疫治疗的新来源

Genetics Research International Pub Date : 2016-02-25 DOI: 10.1155/2016/3451807

Farzaneh Rami, Halimeh Mollainezhad, Mansoor Salehi

{"title":"Induced Pluripotent Stem Cell as a New Source for Cancer Immunotherapy","authors":"Farzaneh Rami, Halimeh Mollainezhad, Mansoor Salehi","doi":"10.1155/2016/3451807","DOIUrl":"https://doi.org/10.1155/2016/3451807","url":null,"abstract":"The immune system consists of cells, proteins, and other molecules that beside each other have a protective function for the host against foreign pathogens. One of the most essential features of the immune system is distinguishability between self- and non-self-cells. This function has an important role in limiting development and progression of cancer cells. In this case, the immune system can detect tumor cell as a foreign pathogen; so, it can be effective in elimination of tumors in their early phases of development. This ability of the immune system resulted in the development of a novel therapeutic field for cancer treatment using host immune components which is called cancer immunotherapy. The main purpose of cancer immunotherapy is stimulation of a strong immune response against the tumor cells that can result from expressing either the immune activator cytokines in the tumor area or gene-modified immune cells. Because of the problems of culturing and manipulating immune cells ex vivo, in recent years, embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) have been used as new sources for generation of modified immune stimulatory cells. In this paper, we reviewed some of the progressions in iPSC technology for cancer immunotherapy.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90448494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Association of RBP4 Genotype with Phenotypic Reproductive Traits of Sows RBP4基因型与母猪表型繁殖性状的关系

Genetics Research International Pub Date : 2016-01-14 DOI: 10.1155/2016/4940532

A. Marantidis, G. Laliotis, M. Avdi

{"title":"Association of RBP4 Genotype with Phenotypic Reproductive Traits of Sows","authors":"A. Marantidis, G. Laliotis, M. Avdi","doi":"10.1155/2016/4940532","DOIUrl":"https://doi.org/10.1155/2016/4940532","url":null,"abstract":"PCR-RFLP was applied to a commercial crossbred pig population in order to investigate the association between polymorphism (SNP) of Retinol-binding protein 4 (RBP4) gene and reproductive performance. 400 sows were genotyped and 2000 records of reproductive traits were used in order to retrieve information about the allele frequencies and the association of the RBP4 gene with main reproductive characteristics of the population. A deviation from the Hardy-Weinberg equilibrium was observed as a result of the AB genotype excess. In addition, the AA genotype saw statistically significant higher values of (i) the total number of born piglets (p < 0.05), (ii) the number of piglets born alive (p < 0.01), and (iii) the number of weaned piglets (p < 0.01). The number of the mummified piglets and the number of the piglets born dead did not differ between the various RBP4 genotypes. Interestingly, the AA genotype had a negative impact (p < 0.05) on the number of piglets born dead, resulting indirectly in a larger litter size. In conclusion, the AA genotype and in extension the A allele of RBP4 gene are in favor of producing larger litter size, suggesting that the RBP4 gene may be used in Marker-Assisted Selection (MAS) programs for a rapid improvement of the reproductive characteristics in pigs.","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4940532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72515354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Molecular Characterization of a Novel Germline VHL Mutation by Extensive In Silico Analysis in an Indian Family with Von Hippel-Lindau Disease. 一个印度Von Hippel-Lindau病家族中一种新的种系VHL突变的分子特征

Genetics Research International Pub Date : 2016-01-01 Epub Date: 2016-03-16 DOI: 10.1155/2016/9872594

Gautham Arunachal, Divya Pachat, C George Priya Doss, Sumita Danda, Rekha Pai, Andrew Ebenazer

{"title":"Molecular Characterization of a Novel Germline VHL Mutation by Extensive In Silico Analysis in an Indian Family with Von Hippel-Lindau Disease.","authors":"Gautham Arunachal, Divya Pachat, C George Priya Doss, Sumita Danda, Rekha Pai, Andrew Ebenazer","doi":"10.1155/2016/9872594","DOIUrl":"https://doi.org/10.1155/2016/9872594","url":null,"abstract":"Von Hippel-Lindau [VHL] disease, an autosomal dominant hereditary cancer syndrome, is well known for its complex genotype-phenotype correlations. We looked for germline mutations in the VHL gene in an affected multiplex family with Type 1 VHL disease. Real-Time quantitative PCR for deletions and Sanger sequencing of coding regions along with flanking intronic regions were performed in two affected individuals and one related individual. Direct sequencing identified a novel heterozygous single nucleotide base substitution in both the affected members tested, segregating with VHL phenotype in this family. This variant in exon 3, c.473T>A, results in substitution of leucine, a highly conserved acid, to glutamine at position 158 [p.L158Q] and has not been reported thus far as a variant associated with disease causation. Further, this variant was not observed in 50 age and ethnicity matched healthy individuals. Extensive in silico prediction analysis along with molecular dynamics simulation revealed significant deleterious nature of the substitution L158Q on pVHL. The results of this study when collated support the view that the missense variation p.L158Q in the Elongin C binding domain of pVHL may be disease causing. ","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2016 ","pages":"9872594"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9872594","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34394808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Leveraging Comparative Genomics to Identify and Functionally Characterize Genes Associated with Sperm Phenotypes in Python bivittatus (Burmese Python). 利用比较基因组学鉴定和功能表征与双维蟒蛇(缅甸蟒蛇)精子表型相关的基因。

Genetics Research International Pub Date : 2016-01-01 Epub Date: 2016-04-20 DOI: 10.1155/2016/7505268

Kristopher J L Irizarry, Josep Rutllant

{"title":"Leveraging Comparative Genomics to Identify and Functionally Characterize Genes Associated with Sperm Phenotypes in Python bivittatus (Burmese Python).","authors":"Kristopher J L Irizarry, Josep Rutllant","doi":"10.1155/2016/7505268","DOIUrl":"https://doi.org/10.1155/2016/7505268","url":null,"abstract":"Comparative genomics approaches provide a means of leveraging functional genomics information from a highly annotated model organism's genome (such as the mouse genome) in order to make physiological inferences about the role of genes and proteins in a less characterized organism's genome (such as the Burmese python). We employed a comparative genomics approach to produce the functional annotation of Python bivittatus genes encoding proteins associated with sperm phenotypes. We identify 129 gene-phenotype relationships in the python which are implicated in 10 specific sperm phenotypes. Results obtained through our systematic analysis identified subsets of python genes exhibiting associations with gene ontology annotation terms. Functional annotation data was represented in a semantic scatter plot. Together, these newly annotated Python bivittatus genome resources provide a high resolution framework from which the biology relating to reptile spermatogenesis, fertility, and reproduction can be further investigated. Applications of our research include (1) production of genetic diagnostics for assessing fertility in domestic and wild reptiles; (2) enhanced assisted reproduction technology for endangered and captive reptiles; and (3) novel molecular targets for biotechnology-based approaches aimed at reducing fertility and reproduction of invasive reptiles. Additional enhancements to reptile genomic resources will further enhance their value. ","PeriodicalId":37545,"journal":{"name":"Genetics Research International","volume":"2016 ","pages":"7505268"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/7505268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34500938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0