Biological Models Research and Technology最新文献

{"title":"3r s applied to in vivo biological activity of recombinant human erythropoietin assay","authors":"Ana Paula Arévalo, Tatiana Basika, Sergio Ancheta, Karen Perelmuter, Alejandro Ricciardi, Mariela Bollati-Fogolín, Martina Crispo","doi":"10.4322/2675-9225.00012023","DOIUrl":"https://doi.org/10.4322/2675-9225.00012023","url":null,"abstract":"An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia ( Ph. Eur. ) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal’s used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system.","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134158163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of general activity and anxietylike behavior in mice following tramadol and meloxicam administration for managing immediate post-operative pain","authors":"A. T. F. B. Antiorio, Jilma Alemán-Laporte, M. S. Garcia-Gomes, Dennis A. Zanatto, P. Yamamoto, Danilo Wadt, L. Cintra, M. Bernardi, C. Mori","doi":"10.4322/2675-9225.00182021","DOIUrl":"https://doi.org/10.4322/2675-9225.00182021","url":null,"abstract":"After analgesic administration, we evaluated general activity in the Open-Field and anxiety-like behavior in the Elevated Plus Maze of vasectomized mice. We divided C57BL/6J male mice into eight groups: saline, three non-operated control groups treated with 10 mg/kg meloxicam, 20 mg/kg tramadol, or both intraperitoneally, and four vasectomized mice groups treated with the same analgesic protocol as the control groups. One group of vasectomized mice received both treatments and an additional 10 mg/kg lidocaine at the incision site. We conducted the vasectomy via scrotal approach under isoflurane inhalation anesthesia and performed behavioral tests after full anesthesia recovery. Mice treated with meloxicam demonstrated low ambulation, spontaneous activity, and rearing frequency. Mice treated with tramadol showed spontaneous behavior compared with the saline control. Due to behavior changes demonstrated by meloxicam controls, we were unable to identify whether meloxicam provided adequate analgesia. Vasectomized mice treated with tramadol showed general activity behavior similar to their control but displayed significantly less rearing, suggesting that they were under potential signs of pain or discomfort. In conclusion, the Open Field test and the Elevated Plus Maze can usefully pre-evaluate analgesic protocols to identify possible interference caused by adverse drug effects. For future directions, an appropriate regimen of meloxicam and tramadol for enhancing mice welfare post vasectomy should be better investigated. exploration to the controls. observed a preference for thigmotaxis in the OFT and reduced time spent in closed arms in the EPM. The vasectomized significantly reduced rearing behavior. Only VM10 and VT20 reduced grooming behavior was a limiting those measures indicate pain or discomfort after the surgical procedure 16,17 our presented a caused by the adverse effects of meloxicam. We of its anti-inflammatory analgesic properties meloxicam pharmacokinetics demonstrated an elimination half-life 38-40 half-life than repeated, stressful adequate active antinociceptive tolerance in Swiss mice meloxicam and present potential dose-dependent antinociceptive effects synergism, reducing doses of both and minimizing the side of","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130171044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teste de preferência entre enriquecimentos ambientais para promoção do bem-estar de fêmeas LG/J","authors":"Natalia Marques Rosa, Nathália Sayuri Servulo Masuki, A. C. Peripato","doi":"10.4322/2675-9225.00072022","DOIUrl":"https://doi.org/10.4322/2675-9225.00072022","url":null,"abstract":"Animal experimentation allows advanced studies in basic and applied research in the most different areas. The use of animals is still necessary to obtain unknown biological responses, especially using animal model such as mice. However, for the research to be carried out satisfactorily, it is necessary to follow ethical procedures, principally by using the humanitarian principle (Reduce, Refine and Replace) and looking for animal welfare. This paper investigated, by using preference test, the environmental enrichment selected by female LG/J mice. Thus, females (n=18) in different stages of development (Pubescents, Virgins Adults","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115017270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Percepção do uso de animais de laboratório entre alunos do ensino médio de 11 escolas da região Centro-Leste do estado de São Paulo","authors":"Sara Bonara Ferreira, Andressa Valim Parca, D. Martins","doi":"10.4322/2675-9225.00172021","DOIUrl":"https://doi.org/10.4322/2675-9225.00172021","url":null,"abstract":"The use of animals is an important step in scientific and technological development in biomedical research. Animal those kept in suitable conditions for scientific purposes can be considered laboratory animals. However, most people are unaware of ethical concepts used in laboratory animals science such as Animal Welfare and the principle of the 3Rs (replacement, reduction and refinement). Dissemination of this knowledge is necessary during the student educational, considering the students as scientific communicators in society. The aimed was evaluate the knowledge of high school students from private and public schools in the in the East-Center region of São Paulo state. The analysis was performed using an online survey form about the laboratory animals science issue of basic knowledge, ethics principles , animal welfare, 3Rs and the use of animals in scientific research. Forty students answered the online survey form. Twenty five percent adolescents did not know the purpose of using animals in research, 90% had never visited or been to a laboratory that works with biomedical","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130151597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scopulariopsis brevicaulis fungal outbreak in laboratory rats","authors":"G. C. A. Mazzarotto, R. Cruz, D. Demolin, R. Gilioli, M. Frajblat","doi":"10.4322/2675-9225.00042022","DOIUrl":"https://doi.org/10.4322/2675-9225.00042022","url":null,"abstract":"This study describes an outbreak of alopecia in laboratory rats caused by the saprophytic fungi Scopulariopsis brevicaulis in a Brazilian lab animal facility. Initially, lesions were identified only in females during the lactation period, which showed circumscribed areas with alopecia without pruritus extensively at the abdomen and the medial side of the thighs. Later during the course of the infection, skin lesions affected both genders reaching about 10% of the colony. Skin scrapings, hair, and environmental samples from the facility were sent for analysis. The cultures were carried out in Sabouraud agar and over seven days fast-growing colonies varying in color from white to brown were observed. Micromorphology showed mycelium and characteristic ringworms of the fungus S. brevicaulis . There is a similar report of an outbreak of this fungus in Turkey. To date, this is the first report in the literature of the fungus S. brevicaulis causing disease in laboratory rats in Brazil. Environmental contamination with S. brevicaulis was observed in the dirty and breeding rooms of the facility. All animals that presented lesions had positive cultures for S. brevicaulis . The presence of S. brevicaulis was also observed in animals without any clinical sign which indicates the possibility of positive carriers in their coat. Similarly, one study reported that the S. brevicaulis was found in 36 animals out of 79 asymptomatic animals analyzed 22 .","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115709120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eficácia do método de graham para o diagnóstico de Syphacia obvelata (Rudolphi, 1802) no monitoramento sanitário de camundongos Swiss Webster de um biotério de criação","authors":"Incerlande Soares dos Santos, Carla Suelania da Silva, Cleber Hooper da Silva, T. O. Silva, Iarine Fiuza, S. Ramos, Lilian Gonçalves de Carvalho, Márcia Cristina Ribeiro Andrade","doi":"10.4322/2675-9225.00092022","DOIUrl":"https://doi.org/10.4322/2675-9225.00092022","url":null,"abstract":"","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121481657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information management in laboratory animal facilities for non-human primates: what we have been using in Brazil","authors":"Natália Francelin Gomes Cordeiro de Lima, K. S. M. Silva, Igor Machado de Castro, T. Kugelmeier","doi":"10.4322/2675-9225.00082022","DOIUrl":"https://doi.org/10.4322/2675-9225.00082022","url":null,"abstract":"In digital curation","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121650794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-capillary lane-maze test in flies: exploratory studies","authors":"Fabiola Boz Eckert, Dhiozer de Brittos Valdati, José Marino, Daniela Cristina de Toni, Cilene Lino de Oliveira","doi":"10.4322/2675-9225.00142022","DOIUrl":"https://doi.org/10.4322/2675-9225.00142022","url":null,"abstract":"One-capillary","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127372826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing an anesthetic protocol for refinement of intracerebral inoculation procedure","authors":"Juliana Ferreira Souza Conceição, Gabriele Bin Alves Pereira, Amanda Siena, C. Mori, Keila Iamamoto Nogi, K. C. S. Ferreira, W. Fahl, E. Mori, K. M. Asano","doi":"10.4322/2675-9225.00192021","DOIUrl":"https://doi.org/10.4322/2675-9225.00192021","url":null,"abstract":"Mouse inoculation test (MIT) is a technique widely used for rabies diagnosis and must be liable to refinement due to animal welfare. The present study aims to compare five different anesthetic associations to stablish a protocol to improve the MIT procedure suitable for animal welfare and safe for a routine of viral isolation in newly weaned mice (3 weeks of age). 80 Swiss-Webster mice ( Mus musculus ) - 40 females and 40 males, 3-week-old, weight ranging from 11 to 14 grams – were used to conduct all procedures. Five anesthetic associations were tested: KX (Ketamine 100 mg/kg and Xylazine 10 mg/kg), KXA (Ketamine 80 mg/kg, Xylazine 5 mg/kg, and Acepromazine 1 mg/kg), KXT (Ketamine 80 mg/kg, Xylazine 5 mg/kg, and Tramadol 5 mg/kg), KXAT (Ketamine 100 mg/kg, Xylazine 10 mg/kg, Acepromazine 2 mg/kg and Tramadol 5 mg/kg) and ATI (Acepromazine 1 mg/kg + Tramadol 5 mg/kg + Isoflurane 5% - 0.5 L/min for induction and 2.5% - 0.5L/min for maintenance). Injectable anesthesia was administered intraperitoneally. We monitored the respiratory rate and body temperature. Response to anesthesia was evaluated according to the induction, surgical anesthesia, and recovery periods. The KXAT and ATI protocols induced surgical anesthesia, with the ATI protocol being the most appropriate and safe to perform the MIT procedure with 100% efficiency, absence of mortality, and rapid recovery of respiratory rate and temperature in the period after the procedure.","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127968023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen supply as a refining technique for injectable anesthesia in laboratory rats","authors":"Jilma Alemán-Laporte, A. Ambrósio, Dennis A. Zanatto, M. S. Garcia-Gomes, A. T. F. B. Antiorio, L. A. Bandini, P. Yamamoto, D. Fantoni, P. Navas-Suárez, Gilbert Alvarado, C. Mori","doi":"10.4322/2675-9225.00142021","DOIUrl":"https://doi.org/10.4322/2675-9225.00142021","url":null,"abstract":"Well-controlled anesthesia is critical to reducing potential surgical complications and ensuring safe and successful procedures. Respiratory depression, inducing hypoxia, and hypercapnia are adverse effects of injectable anesthesia in laboratory rats. This study aimed to determine the effect of oxygen supply in laboratory rats anesthetized with the combination of ketamine (K) and xylazine (X) plus acepromazine (A) or methadone (Me). The results showed that oxygenation allowed adequate levels of SO 2 and paO 2 , avoiding hypoxemia. However, all anesthetized rats showed respiratory acidosis with low pH and high paCO 2 levels, which was not reversed after oxygen administration. The acidosis could be related to hypoventilation due to respiratory depression induced by the XKMe association, as well as absorption atelectasis with the CO 2 accumulation during anesthesia. Despite respiratory acidosis, oxygen administration was beneficial for anesthetized rats preventing hypoxemia. This makes it possible to prevent all the metabolic alterations that cause cell death by hypoxia, improving the well-being of anesthetized rats, as well as the quality of the results obtained.","PeriodicalId":374164,"journal":{"name":"Biological Models Research and Technology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116813811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}