{"title":"Metabolic Profiling and Antibacterial Assessment of Calotropis procera extract against Streptococcus group B","authors":"","doi":"10.34091/ajls.3.1.4","DOIUrl":"https://doi.org/10.34091/ajls.3.1.4","url":null,"abstract":"Calotropis procera is shrub of Asclepiadaceae family and is a source of antimicrobial metabolites against a greater range of bacteria including Streptococcus Group B (SGB). In the present study, different metabolic compounds were extracted from C. procera using 75% methanol. The extract of C. procera was assessed against different bacteria of the SGB family by estimating the zone of inhibition. Moreover, minimal inhibitory concentration (MIC) of different concentrations e.g., 0.001, 0.01, 0.1, 1.0 and 10 mg/mL were determined through zone of inhibition. Gas chromatography mass spectrometry (GC-MS) analysis of plant extract revealed 24 compounds. The most important compounds of the GC-MS array were including undecane, terephthalic acid, Cyclohexane, dimethyl-propane-thiosulfinate, Fluorobenzoic acid Octadecenoic acid and others. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound. The molecular characterization of different bacteria of SGB used in the study revealed the bacteria were consisted of antibiotic resistant genes against.","PeriodicalId":371643,"journal":{"name":"Abasyn Journal Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129540341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunoassay Technique for Diagnosis of Hepatitis B and C Virus Among Drivers and Conductors of long Route Heavy Vehicles","authors":"","doi":"10.34091/ajls.3.1.2","DOIUrl":"https://doi.org/10.34091/ajls.3.1.2","url":null,"abstract":"It is reported from various studies that Drivers and Conductors of heavy vehicles are highly exposed to HCV and HBV. The present study is conducted to investigate the frequency and percentage of HCV and HBsAg in drivers and conductor’s community of heavy vehicles in Pakistan. Data of about 200 subjects from District Bannu, Khyber-Pakhtunkhwa was collected for this study, in which 63% were drivers and 37% were conductors. They were screened for both HCV antibodies and HBsAg. The screening method used during the study was immunochromatographic technique (ICT), which showed that about 19.8% of the drivers were found to be HBsAg positive, while 5.5% were HCV positive. Also, 5.4% of the conductors were found to be HBsAg positive and 4.05% were HCV positive. This study revealed that chances of HCV and Hepatitis B is high in unmarried drivers and conductor as compared to married one. Also, the present study concluded the maximum prevalence of Hepatitis B and C in persons of age 35-45.","PeriodicalId":371643,"journal":{"name":"Abasyn Journal Life Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129642327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunochromatographic Technique for Seropositivity and PCR Based Molecular Diagnosis for Hepatis B and C Virus in a General Population of Mansehra Pakistan","authors":"","doi":"10.34091/ajls.3.1.1","DOIUrl":"https://doi.org/10.34091/ajls.3.1.1","url":null,"abstract":"Hepatitis B and C infections are known to be the major public health issue around the world. Our focus is to investigate the predominance of HBV and HCV infections in the general population of district Mansehra, Khyber Pakhtunkhwa, Pakistan. Blood samples were taken from 652 individuals and were shifted to Alkhedmat laboratory Mansehra and Department of Microbiology Hazara University Mansehra. Serum were isolated from blood samples and screen it by ICT device Kit (Accurate Diagnostics, Houston, Texas, USA). The ICT positive samples were then refined by PCR method. Total 652 individuals [males (368), females (284)] were screened for HBV and HCV infections in which total 37 (5.67%) [males (6.52%) and females (4.57%)] were found positive for HBsAg and 42 (6.44%) [males (7.33%) and females (5.28%)] were found positive for Anti HCV respectively. The ratio of these viral infections is more predominant as compare to other viral infections in Mansehra, Pakistan. The rate of HBV and HCV prevalence is increasing day by day in these areas. Communities with more than 5% HBV and HCV disease, mass vaccination and awareness programs should be undertaken as a matter of urgency.","PeriodicalId":371643,"journal":{"name":"Abasyn Journal Life Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124029207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica","authors":"","doi":"10.34091/ajls.3.1.3","DOIUrl":"https://doi.org/10.34091/ajls.3.1.3","url":null,"abstract":"Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartii and 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds.","PeriodicalId":371643,"journal":{"name":"Abasyn Journal Life Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114353941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and Identification of Fungal Pathogens Associated with Diseases of Onion\u0000Crop in District Swat, Pakistan","authors":"M. Uddin, Nasrullah, Muddasir Khan","doi":"10.34091/ajls.2.2.6","DOIUrl":"https://doi.org/10.34091/ajls.2.2.6","url":null,"abstract":"Onion is an important vegetable and a source of income for formers in\u0000District Swat, Pakistan. However, the crop is attacked by several pathogens\u0000i.e. fungi, bacteria, viruses, and nematodes. Therefore, a study was carried\u0000out for the estimation of fungal diseases of onion crop in district Swat. Within\u0000this study, about 50 samples of diseased onion crop were collected from 17\u0000different regions of District Swat. The most prevalent fungal borne onion\u0000diseases were black mold (38%), onion rust (32%), Blue Mold Rot (10%) and\u0000seedling. Fungal species were consistently isolated from all infected\u0000samples. In which Aspergillus was isolated from 82% samples, Puccinia Alli\u0000was from 70%, Alternaria porri from 52%, Fusarium spp from 42%\u0000Penicillium spp from 40%, Peronospora destructor from 35%, Pyhtaium\u0000from 23% and Rhizoctonia solani from 18% of samples. These fungal species\u0000consistently isolated and cultured from diseased onion plants of different\u0000fields in Swat.\u0000Keywords: Onion crop, Fungal diseases, Aspergillus.","PeriodicalId":371643,"journal":{"name":"Abasyn Journal Life Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129013302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}