New Disease Reports最新文献_第8页

First report of the Fusarium arthrosporioides/avenaceum complex causing olive fruit rot in Italy 节孢镰刀菌/燕麦复合体在意大利引起橄榄果实腐烂的首次报告

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12198

F. Brugneti, S. Turco, M. I. Drais, I. Giubilei, A. Mazzaglia

引用次数: 0

First report of Cocksfoot mottle virus infecting wheat in Estonia 爱沙尼亚小麦感染鸡瘟斑驳病毒的首个报告

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12206

M. Sõmera, P. Sooväli

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Aspergillus niger causing fruit rot disease on rose apple in Malaysia 马来西亚玫瑰苹果果腐病的黑曲霉

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12216

J. R. Al‐Obaidi, N. Rahmad, N. M. Hanafi

{"title":"<i>Aspergillus niger</i> causing fruit rot disease on rose apple in Malaysia","authors":"J. R. Al‐Obaidi, N. Rahmad, N. M. Hanafi","doi":"10.1002/ndr2.12216","DOIUrl":"https://doi.org/10.1002/ndr2.12216","url":null,"abstract":"The rose apple (Syzygium samarangense) is a tropical fruit native to Southeast Asia. Rose apple cultivation occupies c. 3,300 hectares of land in Malaysia with an annual production value exceeding US$9 million (Al-Obaidi et al., 2018). However, fruit rot disease results in the loss of approximately one-third of annual production, causing significant economic hardship. Between 2017 to 2020, rose apples in many farms located in Johor, Malaysia displayed symptoms of fruit rot, characterised by dark, sunken circular lesions on ripening fruits (Figure 1). To identify the causal agent, infected fruit tissues (c. 5 × 5 mm2) were surface sterilised using 1.5% sodium hypochlorite for one minute followed by 70% ethanol for another minute. The tissue was then rinsed with sterile distilled water and placed onto potato dextrose agar (PDA). Plates were incubated at 25°C for seven days and colonies grew that initially appeared white, then turned dark green (Figure 2). Based on the morphological characteristics, particularly the range of conidia sizes (3.0 to 5.4 μm), shape (ellipsoidal) and the colour, the fungus was identified as Aspergillus niger (Silva et al., 2011; Li et al., 2020). For molecular confirmation, the internal transcribed spacer (ITS) region was amplified using ITS1 and ITS4 primers (Santos et al., 2016). The PCR amplicon was sequenced (GenBank Accession No. MF362179) and shared 100% identity with A. niger (MG228418.1, MW188561.1 and KY378943.1). To confirm the pathogenicity of the A. niger isolate (ABI-JJ2), healthy, detached rose apple fruits were surface sterilised by immersing them in a 6% sodium hypochlorite solution for five minutes, followed by washing with 70% ethanol for one minute. Wounded fruits (pin-pricked) were then inoculated with agar plugs containing mycelium of A. niger. Control fruits were inoculated with sterile PDA plugs. Four fruits were used for each treatment. The fruits were placed in plastic boxes and incubated at 25°C with a relative humidity of 80% for seven days. Three days after inoculation, all the inoculated fruits exhibited typical Aspergillus fruit rot symptoms, resembling those seen in the field. The control fruits were unaffected and showed no symptoms. Aspergillus niger was reisolated from the lesions on the inoculated fruit, fulfilling Koch's postulates. Aspergillus niger is responsible for widespread diseases in many plants and is frequently found as a contaminant in food (Lima et al., 2019). This study demonstrates the virulence of A. niger on rose apple, resulting in reduced fruit quality and production. To our knowledge, this is the first record of A. niger causing fruit rot on rose apples in Malaysia, and globally. More research is needed into the pathogenicity and epidemiology of A. niger on rose apples, to aid the development of targeted solutions and limit the effects of this disease. The authors would like to thank the National Institutes of Biotechnology Malaysia and Universiti Pendidikan Sultan I","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of a ‘Candidatus Phytoplasma aurantifolia’ (16SrII‐D) isolate associated with little leaf and stunting of Dracaena reflexa in India 在印度首次报道一种与龙血树小叶和发育迟缓有关的“Candidatus PhytopPlasma aurantifolia”（16SrII-D）分离物

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12194

K. Arunodhayam, N. Mamatha, K. Gopal, G. Sandhya, S. Susmitha, B. Srinivasulu, D. Naik, M. Reddy, B. V. B. Reddy

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First report of Alternaria alternata causing bud and blossom blight on olive in Bosnia and Herzegovina 在波斯尼亚和黑塞哥维那首次报道引起橄榄芽和花枯萎病的互花疫病

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12214

A. Crnogorac, A. Mandić, S. Godena, E. Petrović, S. Matić

{"title":"First report of <i>Alternaria alternata</i> causing bud and blossom blight on olive in Bosnia and Herzegovina","authors":"A. Crnogorac, A. Mandić, S. Godena, E. Petrović, S. Matić","doi":"10.1002/ndr2.12214","DOIUrl":"https://doi.org/10.1002/ndr2.12214","url":null,"abstract":"During 2020 and 2021, symptoms of withering on olive (Olea europaea) followed by necrosis on buds and flowers were observed in the Ljubuški and Mostar regions of western Herzegovina. These symptoms were observed in two of the ten orchards surveyed and on 83 olive (cv. Oblica) trees in the affected orchards (Figure 1), reaching a disease incidence of 60%. Small pieces from lesion margins were plated on potato dextrose agar and after five days' incubation at 25°C under a 12 hr light/dark regime, a single fungus consistently developed. The 10-day-old monoconidial cultures of two isolates (FAZ HZ and KRA HZ) were plated on potato carrot agar (PCA) and DRYES medium for morphological characterisation (Andersen et al., 2001; Simmons, 2007). Developed fungal colonies were olivaceous and cultures grown on PCA produced brown, branched and septate conidiophores with ellipsoid or ovoid conidia. Mean conidial size was 24.8 ±1.4 × 10.1 ±0.6 μm (Figure 2). Observed morphological features identified the fungus as Alternaria section alternata (Woudenberg et al., 2015). Fungal DNA from two isolates was amplified by PCR using primers for ITS (White et al., 1990), rpb2, endoPG, and Alt a 1 (Woudenberg et al., 2015). Amplicons of 540 bp (ITS), 904 bp (rpb2), 460 bp (endoPG), and 492 bp (Alt a 1) were produced from the FAZ HZ and KRA HZ isolates and sequenced (GenBank Accession Nos. OP010267 and OP010268 for ITS, OP038921 and OP038922 for rpb2, OP972863 and OP972864 for endoPG, and OP972865 and OP972866 for Alt a 1, respectively). A BLASTn analysis of these sequences showed 100% identity with the reference strain CBS 117.44 of A. alternata (Fries) Keissler in the ITS (KP124303), rpb2 (KP124772), endoPG (KP124001), and Alt a 1 (KP123854) regions. Phylogenetic analyses were done using the Maximum Likelihood method based on concatenated data set and reference CBS strains using MEGA 11 software (Tamura et al., 2021). Both olive isolates clustered in the same group as A. alternata and were clearly separated from closely related Alternaria species (Figure 3), thus confirming the morphological identification and BLASTn analyses. Thirty three-year-old seedlings of olive cv. Oblica were inoculated by suspension spraying (105 conidia/ml) in three different tests and maintained in a greenhouse for 10 days at 25- 28°C with a photoperiod of 12 hr light/12 hr dark. The same number of control plants were inoculated with sterilised distilled water. Necrotic flowers and buds appeared on infected seedlings, and A. alternata was consistently reisolated (Figure 4). No symptoms appeared on the control plants. This is the first report of A. alternata causing bud and blossom blight on olive in Bosnia and Herzegovina and, to the best of our knowledge, the first report of the fungus on any plant species in the country. Lagogianni et al. (2017) have previously reported A. alternata causing blight on olive in Greece. Since Mostar and Ljubuski are the major olive-producing areas and the increasi","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of Avocado sunblotch viroid in avocado in Kenya 肯尼亚牛油果类日斑病病毒首次报道

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12212

P. Kibwage, E. Avedi, H. Kipkoech, E. Muthomi, M. Oronje, I. Macharia, T. Mutui

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First report of Pyrenophora avenicola causing leaf spot disease on barley 大麦叶斑病病原菌的首次报道

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12213

P. C. C. Rodrigues, P. Angelotti‐Zampar, G. Seron, H. R. Feksa, D. Tessmann

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First report of Snowdrop virus Y and Turnip yellows virus in Narcissus sp. in the United Kingdom Snowdrop病毒Y和萝卜黄病毒在英国水仙花属中的首次报道

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12200

S. Forde, V. Harju, A. Skelton, I. Adams, A. Fowkes, H. Pufal, S. McGreig, C. Conyers, R. Ward, L. Frew, A. Buxton-Kirk, M. Kelly, A. Fox