Experientia supplementum (2012)最新文献_第7页

Inflammasomes in the Kidney. 肾脏中的炎性小体。

Experientia supplementum (2012) Pub Date : 2018-01-01 DOI: 10.1007/978-3-319-89390-7_8

Holly L Hutton, Maliha A Alikhan, A Richard Kitching

引用次数: 9

Specific Systems for Imaging. 成像专用系统。

Experientia supplementum (2012) Pub Date : 2018-01-01 DOI: 10.1007/978-3-319-78259-1_3

Chi Hong Sum, Samantha Marisha Shortall, Jessica Antoinetta Nicastro, Roderick Slavcev

引用次数: 0

Genetics of Inflammasomes. 炎性小体的遗传学。

Experientia supplementum (2012) Pub Date : 2018-01-01 DOI: 10.1007/978-3-319-89390-7_14

Wanessa Cardoso da Silva, Edione C Reis, Telma M Oshiro, Alessandra Pontillo

引用次数: 1

Computational Systems Biology of Metabolism in Infection. 感染代谢的计算系统生物学。

Experientia supplementum (2012) Pub Date : 2018-01-01 DOI: 10.1007/978-3-319-74932-7_6

Müberra Fatma Cesur, Ecehan Abdik, Ünzile Güven-Gülhan, Saliha Durmuş, Tunahan Çakır

引用次数: 6

On the role of co-inhibitory molecules in dendritic cell: T helper cell coculture assays aimed to detect chemical-induced contact allergy. 共抑制分子在树突状细胞中的作用:T辅助细胞共培养检测化学诱导的接触性过敏。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0726-5_9

Matthias Peiser, Manuel Hitzler, Andreas Luch

{"title":"On the role of co-inhibitory molecules in dendritic cell: T helper cell coculture assays aimed to detect chemical-induced contact allergy.","authors":"Matthias Peiser, Manuel Hitzler, Andreas Luch","doi":"10.1007/978-3-0348-0726-5_9","DOIUrl":"https://doi.org/10.1007/978-3-0348-0726-5_9","url":null,"abstract":"T cells play a pivotal role in sensitization and elicitation of type IV allergic reactions. While T helper cells sustain and maintain the differentiation of further effector cells, regulatory T cells are involved in control of cytokine release and proliferation, and T killer cells execute cellular lysis, thereby leading to certain levels of tissue damage. According to their central role, the widely applied and OECD-supported test method for the assessment of the sensitization potential of a chemical, i.e., the local lymph node assay (LLNA), relies on the detection of the immune-responsive proliferation of lymphocytes. However, most sensitization assays recently developed take advantage of the initiators of sensitization, dendritic cells (DCs) or DC-like cell lines. Here, we focus on inhibitory molecules expressed on the surface of DCs and their corresponding receptors on T cells. We summarize insight into the function of CTLA-4, the ligands of inducible co-stimulators (ICOSs), and on the inhibitory receptor programmed death (PD). The targeting of immune cell surface receptors by inhibitory molecules holds some promise with regard to the development of T cell-based sensitization assays. Firstly, a broader and more sensitive dynamic range of detection could be achieved by blocking inhibitors or by removing inhibiting regulatory T cells from the assays. Secondly, the actual expression levels of inhibitory molecules could be also a valuable indicator for the process of sensitization. Finally, inhibitory molecules in coculture test systems are supposed to have a major influence on DCs by reverse signaling, thereby affecting their differentiation and maturation status in a feedback loop. In conclusion, inhibitory ligands of DC surface receptors and/or their cognate receptors on T cells could serve as useful tools in cell-based assays, directly influencing toxicological endpoints such as sensitization. ","PeriodicalId":36906,"journal":{"name":"Experientia supplementum (2012)","volume":"104 ","pages":"115-35"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-3-0348-0726-5_9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31851385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

T cell responses to drugs and drug metabolites. T细胞对药物和药物代谢物的反应。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0726-5_10

C J Earnshaw, T Pecaric-Petkovic, B K Park, D J Naisbitt

引用次数: 7

The use of T cells in hazard characterization of chemical and drug allergens and integration in testing strategies. Foreword. T细胞在化学和药物过敏原危险鉴定中的应用以及测试策略的整合。前言。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0726-5_1

Ian Kimber, Marc Pallardy

引用次数: 2

Using fluorescence to study actomyosin in yeasts. 用荧光法研究酵母中的肌动球蛋白。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0856-9_13

Daniel P Mulvihill

{"title":"Using fluorescence to study actomyosin in yeasts.","authors":"Daniel P Mulvihill","doi":"10.1007/978-3-0348-0856-9_13","DOIUrl":"https://doi.org/10.1007/978-3-0348-0856-9_13","url":null,"abstract":"This year marks the 30th anniversary of the first description of the cellular distribution of actin within a yeast cell. Since then advances in both molecular genetics and imaging technologies have ensured research within these simple model organisms has blazed a trail in the field of actomyosin research. Many yeast proteins and their functions are functionally conserved in human cells. This, combined with experimental speed, minimal cost and ease of use make the yeasts extremely attractive model organisms for researching diverse cellular processes, including those involving actomyosin. In this chapter, current state-of-the-art fluorescence methodologies being applied to yeast actomyosin research, together with an honest appraisal of their limitations, such as the pitfalls that should be considered when fluorescently labelling proteins interacting within a dynamic cytoskeleton, will be discussed. Papers describing the established techniques developed for yeast localisation studies will be highlighted. This will provide the reader with an informed overview of the arsenal of imaging techniques available to the yeast actomyosin researcher and encourage them to consider novel ways these simple unicellular eukaryotes could be used to address their own research questions. ","PeriodicalId":36906,"journal":{"name":"Experientia supplementum (2012)","volume":"105 ","pages":"277-98"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32563041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence methods in the investigation of the DEAD-box helicase mechanism. 荧光法在DEAD-box解旋酶机理研究中的应用。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0856-9_8

Alexandra Z Andreou, Dagmar Klostermeier

{"title":"Fluorescence methods in the investigation of the DEAD-box helicase mechanism.","authors":"Alexandra Z Andreou, Dagmar Klostermeier","doi":"10.1007/978-3-0348-0856-9_8","DOIUrl":"https://doi.org/10.1007/978-3-0348-0856-9_8","url":null,"abstract":"DEAD-box proteins catalyze the ATP-dependent unwinding of RNA duplexes and accompany RNA molecules throughout their cellular life. Conformational changes in the helicase core of DEAD-box proteins are intimately linked to duplex unwinding. In the absence of ligands, the two RecA domains of the helicase core are separated. ATP and RNA binding induces a closure of the cleft between the RecA domains that is coupled to the distortion of bound RNA, leading to duplex destabilization and dissociation of one RNA strand. Reopening of the helicase core occurs after ATP hydrolysis and is coupled to phosphate release and dissociation of the second RNA strand.Fluorescence spectroscopy provides an array of approaches to study intermolecular interactions, local structural rearrangements, or large conformational changes of biomolecules. The fluorescence intensity of a fluorophore reports on its environment, and fluorescence anisotropy reflects the size of the molecular entity the fluorophore is part of. Fluorescence intensity and anisotropy are therefore sensitive probes to report on binding and dissociation events. Fluorescence resonance energy transfer (FRET) reports on the distance between two fluorophores and thus on conformational changes. Single-molecule FRET experiments reveal the distribution of conformational states and the kinetics of their interconversion. This chapter summarizes fluorescence approaches for monitoring individual aspects of DEAD-box protein activity, from nucleotide and RNA binding and RNA unwinding to protein and RNA conformational changes in the catalytic cycle, and illustrates exemplarily how fluorescence-based methods have contributed to understanding the mechanism of DEAD-box helicase-catalyzed RNA unwinding. ","PeriodicalId":36906,"journal":{"name":"Experientia supplementum (2012)","volume":"105 ","pages":"161-92"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-3-0348-0856-9_8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32561475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

T cell responses to contact allergens. T细胞对接触过敏原的反应。

Experientia supplementum (2012) Pub Date : 2014-01-01 DOI: 10.1007/978-3-0348-0726-5_4

Hans Ulrich Weltzien, Stefan F Martin, Jean-François Nicolas

引用次数: 8