Reports of Morphology最新文献

{"title":"Peculiarities of correlations between spirometric and anthropometric indicators in practically healthy young women of mesomorphic somatotype","authors":"Yu. V. Kyrychenko, L. A. Sarafyniuk, O. Khapitska, S. V. Dus, Yulia Yakusheva","doi":"10.31393/morphology-journal-2023-29(3)-09","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-09","url":null,"abstract":"The issue of studying the relationships between indicators of the external structure of the body and spirographic parameters in persons of a certain sex, age, ethno-territorial zoning remains relevant, especially from the point of view of the need to determine the reference values of indicators of external breathing. The purpose of the study was to determine the peculiarities of the relationship between spirographic parameters and indicators of the external structure of the body in practically healthy young women of the mesomorphic constitutional type of the Podilia region of Ukraine. We conducted a complex clinical and laboratory study of young women aged 16 to 20 years, which corresponds to the youthful period of ontogenesis. 109 practically healthy young women were selected, in whom no deviations in the state of health were detected according to the results of radiography, echocardiography, tetrapolar rheovasography and rheoencephalography, sonographic examination of parenchymal organs of the abdominal cavity and thyroid gland, general and biochemical blood analysis. For this group of subjects, we performed a spirographic examination using the Medgraphics Pulmonary Function System 1070 series according to the methodology of the American Pulmonology Association and the European Respiratory Society (2019). The anthropometric study was carried out according to the method of V. V. Bunak (1941), the somatotypological study – according to the calculated modification of the Heath-Carter method (1990). The assessment of the component composition of body weight was carried out according to Matiegka method (1921). After somatotyping, it was found that 32 young women had a mesomorphic type of constitution, for which Spearman’s correlation analysis was conducted in the licensed software package “Statistica 5.5”. It was established that the majority of spirographic indicators in practically healthy young women of the mesomorphic somatotype had isolated statistically significant correlations with anthropo-somatotypological parameters, with the exception of parameters that reflect lung capacities. Vital capacity at rest, had the largest number and strength of correlations, was significantly associated with the value of 24 anthropo-somatotypological indicators. Total, longitudinal and girth body dimensions, the ectomorphic component of the somatotype, muscle, bone and fat mass of the body were most often correlated with the capacity indicators of external respiration. All speed spirographic indicators and maximum peak expiratory flow were correlated with the skinfold thickness. The study of correlations is the basis of further mathematical modeling to determine the appropriate spirographic indicators in an individual representative of the female sex of the juvenile age of the mesomorphic somatotype.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125666108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the microscopic organisation of rat adrenal glands under the influence of Vipera berus berus venom","authors":"Т. S. Niyazmetov","doi":"10.31393/morphology-journal-2023-29(3)-07","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-07","url":null,"abstract":"Snakebite envenoming is a common but neglected public health problem worldwide. Annual mortality as a result of snakebites exceeds 138,000. The organs of the endocrine system are among the first to react to the effects of snake and viper toxins. Under these conditions, the adrenal glands are involved in the pathological process and contribute to the formation of the adaptation syndrome, undergoing complex structural changes. The research aims to study the changes in the microscopic organization of the adrenal glands of rats under the influence of Vipera berus berus venom. Experimental studies were carried out on white non-linear male rats. The animals were conditionally divided into a control and an experimental group of 10 individuals. Experimental rats were injected intraperitoneally in a saline solution with a semi-lethal dose (LD50) (1.576 mg/g-1) of Vipera berus berus venom. Animals of the control group were injected intraperitoneally with only saline solution. Rats were removed from the experiment 24 hours after exposure to the venom and anaesthetized by decapitation. Adrenal gland samples were taken for microscopic examination. Fixation of the material and preparation of paraffin blocks were carried out according to generally accepted methods. Histological preparations of the adrenal glands were stained with hematoxylin and eosin. Histological preparations were studied using an SEO SСAN light microscope. Under the influence of Vipera berus berus venom in zona glomerulosa of the adrenal cortex, moderately pronounced pathological changes were found, including vacuolization and granularity of the cytoplasm of endocrinocytes, loss of precise contours of nuclei, their hyperchromasia, expansion of lumens of sinusoidal capillaries, accumulation of erythrocytes in them. Under these conditions, zona fasciculata is characterized by significant cell granularity and perinuclear edema. Less pronounced structural organization changes were noted in the zona reticularis of the adrenal cortex. Endocrinocytes of this zone had small sizes, eosinophilic cytoplasm and dark nuclei. In the medulla of the adrenal glands, the cells were large in size and had indistinct contours, the cytoplasm was characterized by basophilic granularity, and the nuclei were light due to the predominance of euchromatin. The most pronounced effect of Vipera berus berus venom was on the zona glomerulosa and zona fasciculata of the adrenal cortex; most of the morphological signs of pathology in which were caused by a violation of protein metabolism in the cells of the parenchymal and stromal elements of this organ.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116465071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHYSICAL AND MATHEMATICAL MODELING OF THE DISTRIBUTION OF LOAD FORCES ON THE FEMINAL COMPONENT OF AN ENDOPROSTHESIS OF THE HIP JOINT UNDER REAL CONDITIONS","authors":"V. P. Torchynskyi, T. V. Nizalov, L. V. Shmelyova, A. D. Suprun","doi":"10.31393/morphology-journal-2023-29(3)-08","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-08","url":null,"abstract":"Determination of the factors causing the development of aseptic instability of endoprosthesis components is one of the main tasks of modern traumatology and orthopedics. It is important from a scientific and medical point of view to carry out physical and mathematical modeling of the distribution of load forces and their moments on the femoral component of a hip endoprosthesis. The purpose of the study: to conduct a physical and mathematical modeling of the distribution of load forces on the femoral component of a hip endoprosthesis under real conditions of incomplete axially symmetrical contact of the femoral component of the endoprosthesis and the femur, when the surface of the lower end of the endoprosthesis is not in contact with the surface of the bone. In the work, mathematical modeling of the distribution of point load forces and their moments on the contact surface between the femoral endoprosthesis stem and the bone marrow canal of the femur in real conditions is carried out. For qualitative estimates of point distributions of the load force, based on the analysis of previous results, an estimated empirical formula was obtained for these distributions: g(λ,λ1)=Р(λ,λ1) / 140λ7/2, in which the pressure Р(λ,λ1) is taken in kilograms per square centimeter (kg/cm2), and the point force g(λ,λ1) is in kilograms (kg). It was determined that the best, from the point of view of minimizing the harmful mechanical impact of the prosthesis on the femur, is the situation when the length of the prosthesis stem is not less than half the length of the femur (λ≥0.5). In this case, the values of the point load forces do not exceed 0.1 kg, at least for the length of the area of real contact, which is not less than half the length of the prosthesis stem (λ1≥0.5λ). It has been proven that the use of a prosthesis stem that is less than a third of the length of the femur is not advisable. Since already at the length of the prosthesis stem, which is 30 % of the length of the femur (λ=0.3), point loads increase rapidly and can reach from 0.55 to 1.5 kg depending on the length of the contact area. Such point loads are undesirable for the femur in the area of contact with the prosthesis in terms of the integrity of the femur.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124748400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of nuclear DNA content and cell cycle phases in rat liver cells under chlorpromazine administration","authors":"N. A. Rykalo, O. V. Baylo","doi":"10.31393/morphology-journal-2023-29(3)-04","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-04","url":null,"abstract":"Hepatotoxicity of antipsychotic drugs remains an urgent problem of modern medicine. Therefore, the purpose of the study was to investigate the nuclear DNA content and cell cycle phases of rat liver cells under Сhlorpromazine administration at doses ranging from 3.5 mg/kg to 28 mg/kg for 30 and 60 days. The study was conducted on 60 sexually mature female rats. Chlorpromazine was administered once daily for 30 and 60 days at doses of 3.5 mg/kg, 7 mg/kg, 14 mg/kg, 21 mg/kg and 28 mg/kg. The DNA content in the nuclei of rat liver cells was determined by flow cytometry. Cytological analysis of cells was performed using FloMax software (Partec, Germany), where the percentage of nuclei in the G0G1 interval of the cell cycle, in the S phase, G2M interval, and the apoptosis index – SUB-G0G1 area on DNA histograms were determined. Statistical processing of the results was performed using the Mann-Whitney U test. The results of the study showed that Сhlorpromazine has a dose-dependent hepatotoxic effect: with an increase in the dose of this drug in rats from 7 to 28 mg/kg, the percentage of fragmented nuclei in liver tissue significantly increased, which is a sign of hepatocyte death by apoptosis. It was found that Сhlorpromazine at a dose of 3.5 mg/kg did not increase hepatocyte apoptosis, while at a dose of 21 and 28 mg/kg the drug showed the highest hepatotoxicity, increasing the level of apoptosis by 1.9 and 2.1 (p˂0.05) times, respectively. The hepatotoxic effect is enhanced by the use of Сhlorpromazine for 60 days, which is manifested in a significant increase in hepatocyte nuclear DNA fragmentation, which, in our opinion, should be taken into account when conducting long-term therapy in patients.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129424449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological reasoning of the efficiency of application “Iruksan” in the experiment","authors":"S. D. Khimich, V. P. Rautskis","doi":"10.31393/morphology-journal-2023-29(3)-05","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-05","url":null,"abstract":"Treatment of infected and purulent wounds remains an actual problem nowadays. Scientific and practical interest is caused by the use of collagenase enzyme for wound debridement. Aim: to study morphological changes in contaminated and purulent wounds when using “Iruksan” ointment containing collagenase. To reproduce a wound infection, the wound was contaminated with a pathogenic strain of Staphylococcus aureus in combination with Pseudomonas aeruginosa. Microbiological, histological and statistical studies were conducted during the experiment. Control of contamination and identification of pathogens took place in all rats after contamination before use of ointment and at the day of exclusion from experiment. Due to microbiological findings, number of microorganisms in the wounds of animals of the control group remained practically unchanged (p>0.05) until the 10th day of observation, and in the experimental group (“Iruksan” ointment was used in the treatment of wounds), the number of microorganisms in the wounds has already decreased by the 7th day of the experiment (p<0.05). The obtained data correlate with the results of a morphological findings, especially with the severity of the inflammatory process. We noticed reduction of signs of the inflammatory process and the improvement of epithelization of the wound defect in the group of animals that were treated with “Iruksan” ointment containing collagenase. Due to the results of histological studies, complete coverage of the wound surface with newly formed epithelium was revealed on the 7th day of the experiment in the experimental group, in contrast to the control group where, on the 7th day, typical signs of the wound process for this time period were determined. In the control group of animals, epithelization of wounds was observed on the 10th day. The obtained data demonstrate expedience of use collagenase-based ointment for the treatment of wounds in the first phase of the wound process. It results in faster wound bed cleaning from necrotized tissues and enhance epithelialization.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116387138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in markers of collagen metabolism in the blood serum of white rats during the filling of femur defects with implants based on polylactide and tricalcium phosphate with allogeneous mesenchymal stem cells","authors":"N. Gontar","doi":"10.31393/morphology-journal-2023-29(3)-02","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-02","url":null,"abstract":"The use of synthetic materials in combination with osteogenesis stimulators is one of the advanced directions of the development of traumatology. The purpose of the study: based on the analysis of biochemical markers of collagen metabolism in the blood serum of laboratory rats with a femur defect, to evaluate the course of bone remodeling after filling the defect with implants based on polylactide and tricalcium phosphate with simultaneous and delayed administration of allogeneic mesenchymal stem cells (MSCs). On the model of a defect in the metaphysis of the femur in white rats, the indicators of collagen exchange in blood serum were studied: the content of protein-bound, free fractions, the amount of hydroxyproline (HOP), the ratio of the content of protein-bound HOP to free PB/F was calculated. Comparison of the results of different groups was performed using the Student-Fisher method. The difference was considered statistically significant if p<0.05. Filling the defect with implants led to a decrease in the content of protein-bound HOP by 16.69 % and 14.34 % on the 15th and 90th days (p<0.05), an increase in the content of free HOP by 74.96 %; 67.31 % and 56.74 % (p<0.001), the content of the amount of HOP by 25.37 %; 23.66 % and 18.28 % (p<0.05), and reduction of PB/F by 52.20 %; 49.90 % and 45.30 % (p<0.001) relative to intact on the 15th; 30th and 90th days. Addition of MSCs to the implants at the same time as the installation caused a decrease in the content of free HOP by 63.90 %; 54.63 % and 42.76 % on the 15th; on the 30th and 90th days (p<0.001), the total metabolite by 21.87 % and 18.58 % on the 15th and 30th days (p<0.05) and a decrease in PB/F by 47, 50 %; 43.20 %; 37.60 % on the 15th; on the 30th and 90th days (p<0.001) relative to the intact. Postponing the introduction of MSCs increased the content of free HOP by 40.48 % (p<0.001); 20.49 % and 16.58 % (p<0.05) with a lower PB/F by 38.20 %; 25.80 % and 23.40 % (p<0.05) on the 15th; 30th and 90th days relatively intact. When the defect was filled with implants without MSCs, a moderate inhibition of anabolism and rapid activation of collagen destruction was observed. With the simultaneous use of implants and MSCs, the rate of formation and intense destruction of collagen was observed. When the introduction of MSCs was delayed, a moderate rate of destruction was recorded, which most contributed to collagen metabolism.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115824958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of immediate adaptation of the circulatory system to static load in persons with different body mass index","authors":"V. Pastukhova, O. O. Bakunovsky, S. B. Drozdovska, M. M. Filippov, V. M. Ilyin, S. Krasnova, T. Oliinyk","doi":"10.31393/morphology-journal-2023-29(3)-06","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-06","url":null,"abstract":"In the vast majority of sports, muscle activity is dynamic in nature, however, recently sports physiologists have been paying considerable attention to static exercises. The deep mechanisms of the phenomenon of static efforts have been studied since its discovery, but there are still many gaps in biological knowledge regarding the issue of the impact of static load on the functioning parameters of the circulatory system in individuals with different anthropometric characteristics. The purpose of the work is to study the influence of dosed static load on the parameters of the circulatory system during the early recovery period in people with different body mass indexes. During the study, 36 young men were examined, who were divided into two groups according to the body mass index (BMI) criterion – young men with a normal BMI (nBMI group, the average value of BMI in the group – 21.6), as well as young men with an increased BMI (group iBMI, the average value of BMI in the group is 28.1). Static load (SL) was modeled by maintaining a force equal to 50 % of the maximum static force for 15 seconds on a static dynamometer DS-200. Before exercise and within 3 minutes after its completion, the main parameters of central hemodynamics were measured in the examined persons using tetrapolar thoracic rheoplethysmography on the computerized diagnostic complex \"Cardio+\" (Ukraine). Statistical data processing was carried out using the computer program IBM SPSS Statistics (version 26), using non-parametric methods of evaluating the obtained results. We have established that in the initial state, young men with a normal body mass index differ from persons with an increased body mass index by a lower value of heart rate, total peripheral resistance and blood pressure, as well as higher values of stroke and minute blood volumes, stroke and cardiac indices. The dosed static load leads to the same nature of changes in the parameters of the circulatory system in all examined persons, regardless of the body mass index. Differences in body mass index affect only the degree of manifestation of certain changes. A more significant manifestation of the Lingard phenomenon was recorded in young men with an increased body mass index, as evidenced by a greater degree of deviation of the measured parameters of the circulatory system in response to static exercise.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"293 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116250209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations of the dermatoscopic index with anthropometric and somatotypological parameters of men with benign nevi","authors":"N. B. Y. Haddad, H. V. Chaika, І. М. Kyrychenko, О. М. Shapoval, V. G. Dronenko","doi":"10.31393/morphology-journal-2023-29(3)-01","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-01","url":null,"abstract":"Malignant transformation of benign nevi is one of the factors that leads to increased scientific interest in the study of the pathogenesis of their occurrence, the influence of external factors on them, etc. One of the still unsolved issues is the study of the relationship between the features of nevi indicators and the anthropometric parameters of the human body. The purpose of the work is to investigate the peculiarities of correlations of the dermatoscopic index with anthropometric and somatotypological indicators of Ukrainian men with benign nevi. A clinical, laboratory and pathogistological examination of 34 Ukrainian men of first adulthood with melanocytic benign simple nevi, 27 with melanocytic benign dysplastic nevi, 14 with melanocytic benign congenital nevi, and 17 with non-melanocytic benign nevi was carried out. The dermatoscopic index was calculated according to the “ABCD rule of dermatoscopy”. Anthropometric examination was carried out according to Bunak’s scheme. The Heath-Carter mathematical scheme was used to assess the somatotype. Matiegka formulas were used to calculate fat, bone and muscle components of body mass. In addition, the muscle component of body weight was estimated according to the method of the American Institute of Nutrition. The assessment of correlations between the dermatoscopic index and body parameters was carried out in the license package “Statistica 6.0” using the non-parametric method of Spearman. An analysis of multiple reliable and average strength of unreliable correlations between the value of the dermatoscopic index and anthropo-somatotypological indicators in men with benign nevi was performed. In men with simple melanocytic nevi, only moderately strong direct (r = from 0.30 to 0.34), mostly unreliable, correlations with all sizes of the pelvis were established. In men with melanocytic dysplastic nevi, inverse correlations of medium strength, mostly reliable (r = from -0.38 to -0.52) were established with all dimensions of the pelvis and almost all transverse dimensions of the trunk, as well as with almost half of the indicators of the skinfold thickness. In men with melanocytic congenital nevi, direct, mostly unreliable (r = from 0.30 to 0.47) correlations were established with all dimensions of the pelvis, with almost all transverse dimensions of the trunk, with almost half of the girth of the body and indicators of the skinfold thickness and almost all indicators of the component composition of body weight. In men with non-melanocytic nevi, direct, mostly unreliable (r = from 0.30 to 0.47) correlations were established with all transverse dimensions of the trunk and pelvis, most of the total and girth dimensions of the body. Quantitative analysis of reliable and average strength of unreliable correlations between the value of the dermatoscopic index and anthropo-somatotypological indicators was also carried out. The established features of the correlations expand modern ideas about the risk of be","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121962592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural changes in the heart tissue of rats under conditions of acute intoxication with Vipera berus berus venom","authors":"О. Maievskyi, А. М. Bobr, I. V. Gunas","doi":"10.31393/morphology-journal-2023-29(3)-03","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-03","url":null,"abstract":"Among all poisonous animals, snakes attract the special attention of mankind. Cases of poisoning by their toxins are extremely common and are an unsolved public health problem worldwide. It has been established that among a number of complications due to snakebites, an important role is played by disturbances in the normal functioning of the organs of the cardiovascular system. The aim of the research is to study the features of histological changes in the heart tissue of rats under conditions of acute intoxication with Vipera berus berus venom. Experimental studies were carried out on white non-linear male rats. The animals were conditionally divided into two groups – a control and an experimental group of 10 individuals each. Experimental rats were injected intraperitoneally with a semi-lethal dose (LD50) (1.576 mg/g-1) of Vipera berus berus venom in saline solution. Animals of the control group were injected intraperitoneally with only saline solution. Rats were removed from the experiment 24 hours after exposure to the venom, anesthetized by cervical dislocation. Heart samples were taken for microscopic examination. Fixation of the material and preparation of paraffin blocks were carried out according to generally accepted methods. Histological heart preparations were stained with Picro Sirius Red/Fast Green. Histological preparations were studied using a SEO SСAN light microscope. Thus, under the conditions of acute intoxication with Vipera berus berus venom in experimental rats, microscopic examination of the heart tissue revealed pathological shifts in the structural organization of all layers of the organ. In the epicardium, an increase in the volume of collagen fibers and thickening of vessel walls were characteristic. The most pronounced histological changes were observed in the myocardium. Swelling, disorganization of muscle fibers, their fragmentation, lysis, destruction, loss of characteristic transverse striations, necrosis were detected in it. In addition, an increase in the number of fibroblasts, their activation and, as a result, the growth of connective tissue components, including collagen fibers, in the myocardial tissue were revealed. The endocardium of experimental rats was distinguished by desquamation of the endothelial lining and an increase in the number of active fibroblasts in the outer connective tissue layer.","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"12 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126793656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of anthropometric indicators and dimensions of the coronary sinus and relationships between these indicators in patients without coronary artery pathology","authors":"Ye. M. Liulka, S. Bilash","doi":"10.31393/morphology-journal-2023-29(3)-10","DOIUrl":"https://doi.org/10.31393/morphology-journal-2023-29(3)-10","url":null,"abstract":"Recently, in Ukraine, there has been a significant increase in the possibilities of invasive treatment of cardiovascular pathologies, the number of open-heart cardiosurgical operations and endovascular interventions is increasing. Each of these interventions requires endovascular catheterization of the coronary venous sinus of the heart, the variety of anatomical features of which requires a detailed study of the anatomy of the venous system of the heart. The purpose of the work is to establish the features of the coronary sinus dimensions and anthropometric indicators in men and women without coronary artery pathology, as well as the relationships between these indicators. Weight, body length, body mass index, anterior-posterior size of the chest, as well as the dimensions of the coronary sinus were determined (the length of the coronary sinus from the mouth to the oblique atrial vein, the transverse size of the coronary sinus in the area of the mouth in the sagittal and axial planes, the transverse size of the coronary sinus in the middle third in the sagittal and axial plane, the transverse dimension of the coronary sinus at the level of the oblique vein of the atrium in the sagittal and axial plane) in 15 men and 9 women aged 44 to 60 years (average age according to the age classification of the World Health Organization) without pathology of the coronary arteries, who underwent CT coronary angiography at the “Amosov National Institute of Cardiovascular Surgery NAMS of Ukraine”. Statistical processing of the obtained results was carried out in the license package “Statistica 6.0” using non-parametric estimation methods. As a result of the conducted research, the limits of the percentile range of anthropometric indicators and the size of the coronary sinus in men and women without coronary artery pathology were established. When analyzing the value of these indicators between men and women, only significantly higher values of body mass and length were established in men. Therefore, when analyzing the relationship between the dimensions of the coronary sinus and the anthropometric parameters of the body, the distribution of indicators by sex was not carried out. When analyzing the correlations between age, sex, weight, body length, body mass index, signs of body mass index (indicates underweight, equivalent to normal body weight, indicates the presence of excess weight, or is a sign of obesity) and the anterior-posterior size of the chest with almost all dimensions of the coronary sinus, multiple reliable direct strong (r=0.64 and r=0.67) and medium strength (r=0.44 and r=0.47), as well as unreliable medium strength direct correlations with the anterior-posterior size of the chest were established (r=0.40 in both cases). In addition, multiple nonreliable average strength direct correlations of more than half of coronary sinus dimensions were established with body weight (r= from 0.32 to 0.35). When analyzing the correlations between the sizes o","PeriodicalId":364875,"journal":{"name":"Reports of Morphology","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128500706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}