Clinical and Experimental Morphology最新文献

{"title":"Endometrial implantation failure in cycles of in vitro fertilization in patients with chronic endometritis","authors":"T. Tral, G. Tolibova, I. Kogan","doi":"10.31088/cem2023.12.1.24-33","DOIUrl":"https://doi.org/10.31088/cem2023.12.1.24-33","url":null,"abstract":"Introduction. Chronic endometritis is one of the most frequent gynecological diseases which negatively impacts the main pathogenetic links in the morphogenesis of secretory and gestational endometrial transformation. These links determine implantation failure. The study aimed to verify the expression of estrogen receptors (ER) and progesterone receptors (PR), progesterone-induced blocking factor (PIBF) and stromal cell-derived factor-1 (SDF-1) in the endometrium of patients having chronic endometritis with ineffective IVF cycles or undeveloped pregnancies occurred after assisted reproductive technologies in their histories. Materials and methods. We formed 3 study groups: group I included samples of the endometrium of patients with ineffective IVF cycles (n=50); group II contained samples received from patients with a history of undeveloped pregnancy after IVF (n=50); and group III (the control group) included endometrial tissue from healthy patients (n=25). We carried out routine histological evaluation followed by an immunohistochemical assay with ER, PR, PIBF, and SDF-1 in the endometrial glands and stroma in the middle secretion phase. esults. The middle stage of the secretion phase of the menstrual cycle in endometrial samples of groups I and II was detected in only 46% and 42% of cases, respectively. We showed a decrease in the ER and PR expression in more than 92% of cases. Statistically significant decrease was detected in PIBF and SDF-1 expression in the endometrial glands and stroma within the implantation window in patients with a history of reproductive loss. Conclusion. Violation of implantation viability with an imbalance of chemokines, cytokines, cellular factors, and structural and functional characteristics of the endometrium was found in patients with revealed chronic endometritis and history of ineffective IVF cycles and miscarriage after ART. Keywords: endometrium,IVF, missed abortion after IVF, estrogen receptor, progesterone receptor, progesterone-induced blocking factor, stromal cell-derived factor-1","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69280919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and morphological diagnosis of eosinophilic esophagitis","authors":"K.S. Maslenkina, L.M. Mikhaleva, E.N. Motylev, M.U. Gushchin, V.O. Kaibysheva Kaibysheva, D.A. Atyakshin, Y.Y. Kudryavtseva, G.Y. Kudryavtsev","doi":"10.31088/cem2023.12.3.5-18","DOIUrl":"https://doi.org/10.31088/cem2023.12.3.5-18","url":null,"abstract":"Eosinophilic esophagitis (EoE) is an immune-mediated disease that presents with dysphagia and esophageal bolus obstruction and is characterized by predominant intraepithelial eosinophilic infiltration at histology. The aim of our literature review was to delineate clinical and morphological EoE features in terms of differential diagnosis with other esophageal diseases, i.e., gastroesophageal reflux disease (GERD), inflammatory bowel diseases (IBDs), autoimmune diseases, and rare esophageal disorders. Clinical features of dysphagia and esophageal bolus obstruction and endoscopic criteria (according to EREFS) are typical of EoE. Nevertheless, eosinophilia of the esophageal mucosa is not a specific marker of mucosal injury and is not sufficient for EoE diagnosis. Therefore, eosinophilic esophagitis histological scoring system (EoEHSS) was developed. It involves assessment of intraepithelial eosinophilic infiltration, basal zone hyperplasia, eosinophilic abscesses, eosinophil surface layering, dilated intercellular spaces, surface epithelial alteration, dyskeratotic epithelial cells, and lamina propria fibrosis. Complex clinical, endoscopic, and histological evaluation allows accurate diagnosis of EoE and other causal factors of esophageal eosinophilia. Keywords: eosinophilic esophagitis, esophageal eosinophilia, gastroesophageal reflux disease, celiac disease, inflammatory bowel diseases, achalasia, atopy","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135840831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and morphological assessment of uterine scars after cesarean section in patients with gynecological and extragenital diseases","authors":"N.M. Markaryan, R. Vandysheva, N. Nizyaeva, Z. Gioeva, S. A. Mikhalev, M. Khamoshina, L. Mikhaleva","doi":"10.31088/cem2023.12.1.34-45","DOIUrl":"https://doi.org/10.31088/cem2023.12.1.34-45","url":null,"abstract":"Introduction. The article studies excised scars on the uterus after Gusakov’s and Stark’s cesarean sections, with an assessment of the impact of gynecological and extragenital diseases on the viability of the scar. Cesarean scar pregnancy is known to be a frequent indication for surgical delivery. However, if the uterine scar is stable, it is advisable to deliver the baby through the natural birth canal. At present, natural delivery of pregnant women with a scar on the uterus through natural childbirth is an important task in modern obstetrics. Thus, the aim of our study was to perform a multifaceted clinical and morphological evaluation of uterine scars after cesarean section in patients with gynecological and extragenital diseases. Materials and methods. We analyzed samples of scar tissue on the uterus after cesarean section performed in 68 patients. A pathomorphological study was carried out with Mallory staining with hematoxylin and eosin. Immunohistochemical study was performed with antibodies to collagen IV, vimentin, desmin, and von Willebrand factor. Results. In 56 out of 68 puerperas (82.3%), the scars on the uterus were stable with complete replacement of the defect with muscle (63.2%) or connective tissue (19.1%), regardless of the operation duration and the suturing technique. This was confirmed by additional diagnostic methods (Mallory stain and IHC with an antibody panel). We found that connective tissue dysplasia, which is indirectly indicated by the presence of myopia in 5 patients with wealthy fibrous scars, can affect excessive connective tissue in the scar. Twelve out of 68 puerperas (17.6%) had the scars (according to pathomorphological criteria) with severe edema, hemorrhages, tissue with different fibers, and uneven thinning. These patients had extragenital diseases, such as type 2 diabetes mellitus (36.4%) and anemia (18.2%). We did not reveal any influence of existing gynecological diseases on the scar stability. Conclusion. The healing of the postoperative uterine wound was mostly influenced by extragenital diseases (type 2 diabetes mellitus, anemia, connective tissue dysplasia) associated with metabolic disorders and having systemic effects on the body. The Gusakov’s or Stark’s incision closure techniques did not affect the quality of the scar. However, the scars after cesarean section using the Stark incision technique had significantly more connective than muscular tissue. Keywords: cesarean section, incompetent uterine scar, type 2 diabetes mellitus, anemia, connective tissue dysplasia, pathological examination, immunohistochemical study","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69281035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the content of ATP synthase in brain neurons during experimental cerebral ischemia","authors":"E. V. Uzlova, S. Zimatkin, E. Bon","doi":"10.31088/cem2023.12.1.68-76","DOIUrl":"https://doi.org/10.31088/cem2023.12.1.68-76","url":null,"abstract":"Introduction. ATP synthase is a key component of ATP synthesis. The study of its content in brain neurons in experimental cerebral ischemia may reflect changes in the functional state of different neurons and their sensitivity to the pathological effect. The study aimed to reveal patterns in ATP synthase immunoreactivity in neurons of various parts of the rat brain during subtotal ischemia of various durations. Materials and methods. Modeling of subtotal cerebral ischemia (SCI) was carried out by ligation of both common carotid arteries (n=12: control group – n=4, 30-minute SCI – n=4, 3-hour SCI – n=4); the control animals underwent a sham surgery. Frontal paraffin sections were stained according to the Nissl method to identify brain structures and immunohistochemically for ATP synthase. ATP synthase immunoreactivity was expressed in units of optical density ×103. Results. Changes in the immunoreactivity of ATP synthase in brain structures occur to varying degrees and not in all studied structures. The most pronounced and rapid decrease in the content of ATP synthase was observed in telencephalon, namely in the temporal and retrosplenial agranular cortex. The least pronounced changes in the cortex were in the structures of the hippocampus, in the neurons of layer II of the CA2 field. In the structures of the thalamus, hypothalamus, and midbrain the dynamic varied. In the structures of the medulla oblongata, the decrease in immunoreactivity occurs more slowly and less pronounced. Conclusion. During experimental cerebral ischemia the degree and rate of change in the content of ATP synthase in rat brain structures vary greatly and depend both on the brain region and the neurotransmitter nature of neurons. Keywords: ATP synthase, ischemia, immunohistochemistry, brain, rat","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69281117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of neuroendocrine cells and splenic macrophages to tumor development in the colon","authors":"M.N. Mikhailova, O.M. Arlashkina, G.Yu. Struchko, L.M. Merkulova, I.S. Stomenskaya, O.Yu. Kostrova","doi":"10.31088/cem2023.12.3.72-81","DOIUrl":"https://doi.org/10.31088/cem2023.12.3.72-81","url":null,"abstract":"Introduction. High cancer incidence requires finding new ways for comprehensive studying car-cinogenesis. Therefore, it is crucial to understand immune organ cell response and cell interaction in tumor development. The aim of the research was to study Synaptophysin+-, CD68+-cells, and biogenic amines in rat spleens during tumor development in the colon during dysplasia stages and adenocarcinoma formation. Materials and methods. Spleen histological slides of 110 mature male rats were studied 1 and 4 months after 1,2-dimethylhydrazine carcinogen administration using immunohistochemical, morphometric, and luminescent histochemical methods. Results. We found imbalanced production of biogenic amines (serotonin, histamine, and catecholamines) in the spleen and, therefore, a decrease in the cellular activity of the germinal centers of the lymphoid nodules. We also observed activation of periarteriolar lymphoid sheath (PALS) and red pulp in rats with precancerous colon lesions (1 month after carcinogen administration). At the same time, there was an increase in the number of CD68+ macrophages and Synaptophysin+ cells in the red pulp. In animals with adenocarcinoma (4 months after carcinogen introduction), the level of catecholamines in the luminescent granular cells of the PALS and the functional activity of these cells increased significantly. Simultaneously, the number of macrophages decreased in all the studied spleen compartments. Amid the decreased level of all biogenic amines in the red pulp, the quantity of Synaptophysin+ cells grew even more. Conclusion. The cells of all spleen compartments react to colon carcinogenesis, with reactivity of PALS cells and the red pulp being the most pronounced. The population of spleen macrophages undergoes rapid changes: their number increases in the red pulp in animals with precancerous lesions, while it decreases in all the splenic structures of rats with adenocarcinoma. Synaptophysin+ neuroendocrine cells of the red pulp play an important role in the reaction of the spleen to tumor development, and the number of these cells rises over time. Biogenic amines participate in the interaction of spleen cells with each other and with tumor-associated cells. Keywords: spleen, biogenic amines, neuroendocrine cells, synaptophysin, carcinogenesis","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135840352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological features of the internal organs in mice after prolonged microplastics consumption","authors":"N.A. Zolotova, D.Sh. Dzhalilova, I.S. Tsvetkov, A.V. Sentyabreva, O.V. Makarova","doi":"10.31088/cem2023.12.3.82-92","DOIUrl":"https://doi.org/10.31088/cem2023.12.3.82-92","url":null,"abstract":"Introduction.Microplastics (MP) pollute the environment and can pose a danger to living organisms including humans. Experimental studies on mice and rats demonstrated that microplastics can enter the internal environment, causing structural damage to and dysfunction of various organs. However, data on this issue are scarce and contradictory. The aim of this paper was to characterize morphological changes in the internal organs in mice after prolonged MP consumption in different doses. Materials and methods. We formed four groups (n=5 each): three experimental and one control group. Mice of the experimental groups were given a 5-μm polystyrene particle suspension in distilled water at a concentration of 0.1, 1, and 10 mg/l for 4 weeks. The daily consumption doses averaged 0.023, 0.23, and 2.3 mg of microparticles per kg of animal body weight, respectively. The control group received distilled water throughout the experiment. We conducted a morphological examination of the colon, spleen, liver, kidneys, lungs, thymus, mesenteric lymph nodes, brain, heart, testicles, and thigh muscles. In the colon, morphometric methods were used to evaluate inflammatory infiltration, the number of endocrine and goblet cells, and the content of neutral and highly sulfated mucins in the latter. Results. On morphological examination under the influence of 5-μm polystyrene particle consumption in different doses, we revealed pathological changes only in the spleen and colon. However, a decrease in the goblet cell volume fraction in the colon mucosa was found in the group with low dose of microplastics (0.023 mg/kg/day). The group that consumed 2.3 mg/kg/day of microplastics showed the activation of both spleen compartments, an increased endocrine cells count, and a decreased proportion of highly sulfated mucins in goblet cells. Conclusion. Polystyrene microparticles with a 5-μm diameter consumed for 4 weeks at doses of 0.023–2.3 mg/kg/day cause adaptive morphological and functional changes in the colon and spleen. Keywords: microplastics, polystyrene, spleen, colon, internal organs","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135841703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astrocyte lineage differentiation profiles of the fetal human telencephalon","authors":"A.S. Kharlamova, E.G. Otlyga, O.S. Godovalova, O.A. Junemann, S.V. Saveliev","doi":"10.31088/cem2023.12.3.50-63","DOIUrl":"https://doi.org/10.31088/cem2023.12.3.50-63","url":null,"abstract":"Introduction. Brain functioning is kept by both neuronal cell activity and macroglia, i.e., astrocytes and oligodendrocytes. The current data on the prenatal development of the human brain are scarce, and gliogenesis is less studied than cortical neurogenesis. Normal limits and variations and spatiotemporal patterns of glial differentiation in human brain development remain poorly studied. Materials and methods. We used human fetal autopsy samples from the Collection of the Laboratory of Nervous System Development of Avtsyn Research Institute of Human Morphology. For immune and morphological analysis, samples of 38 fetal cerebral hemispheres at stages from 8 postconceptional weeks to birth were chosen. Results. We provided the results of the pilot comparative immune and morphological study with the panel of markers (GFAP, ALDH1L1, FABP-7) of the fetal human telencephalon in prenatal ontogenesis. Specific differentiation and maturation of the astrocyte population on the telencephalon start before early fetal period (12–13 gestational weeks). GFAP+ and ALDH1L1+ astrocyte populations in early human telencephalon are still to be studied for their homology. Analysis of GFAP+ and ALDH1L1+ glioblast distribution proposes dorsal proliferative zone as a source for fibrous cortical astrocytes. Comparative immune and morphological analysis of FABP-7+ neuroblasts in the fetal telencephalon questions whether FABP-7 cells belong to astrocyte population at early prenatal human ontogenesis. Conclusion. In the telencephalon, temporal and/or spatiotemporal translational profiles of these three antigens differ, which indicates that the astrocyte population is heterogeneous in early ontogenesis. Keywords: human brain development, telencephalon, glial differentiation, astrocytes, astrocyte fate lineage, GFAP, ALDH1L1, FABP-7","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135844568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant expression of p53 in gastric carcinoma and its association with HER2 status","authors":"N. Danilova, A. Chayka, V. Khomyakov, N. Oleynikova, V. Kakotkin, D. Vychuzhanin, Y. Andreeva, P. Malkov","doi":"10.31088/cem2023.12.1.46-58","DOIUrl":"https://doi.org/10.31088/cem2023.12.1.46-58","url":null,"abstract":"Introduction. p53 is a tumor suppressor, whose expression is actively studied in many tumors. However, scientists argue about aberrant p53 expression criteria and whether tumoral p53 expression correlates with various clinical and morphological parameters of gastric cancer and has a prognostic role. No data were published in Russia on the incidence of tumors with p53 overexpression. It remains unclear whether aberrant p53 expression is an independent prognostic sign in gastric cancer. The study aimed to evaluate the frequency of p53 expression in gastric adenocarcinomas in the Russian population, to give the definition for aberrant p53 expression, and to clarify the relationship between p53 expression, clinical and morphological tumor characteristics, HER2 status, and the impact of p53 expression on the prognosis. Materials and methods. We studied surgical pathology samples from 310 patients with verified gastric cancer. The age of the patients ranged from 22 to 85 years (mean 63 years). Each sample was stained immunohistochemically with antibodies to p53 (clone DO-7) and the HercepTest kit (Dako/Agilent Technologies). We compared the results with the main clinical and morphological characteristics of gastric cancer and patient survival data. Results.The frequency of aberrant p53 expression was 49.4%. Aberrant expression should include both cases with overexpression and cases with a complete absence of marker expression. In contrast to the normal p53 expression group, the group with aberrant p53 expression was characterized by more frequent proximal tumor location; fewer diffuse and infiltrative carcinoma forms; a significant predominance of tubular, papillary, and mixed histological types (p=0.000); significantly higher degrees of differentiation (p=0.011); a significantly lower number of cases with identified signet ring cells in tumors (p=0.000); a lower frequency of stage IV in patients; and a significant predominance of the intestinal and intermediate subtype according to P. Lauren classification (p=0.000). The overall five-year survival in patients with aberrant p53 expression was not significantly lower (p=0.392) than in patients with normal p53 expression (35.6%, median 36 months and 42.5%, median 51 months, respectively). According to the Cox proportional hazards regression model, the p53 expression level is not a significant prognostic sign (HR=1.281; CI: 0.818–2.008, p=0.280). In the group with aberrant p53 expression, a positive HER2 status was detected significantly more often (13.7%) than in the group with normal p53 expression (1.9%, p=0.000). Conclusion. The group with aberrant p53 expression can be considered as an immunohistochemical analog of the chromosomally unstable gastric cancer subtype (according to TCGA) and the MSS/TP53- subtype (according to ACRG). In the group with aberrant p53 expression, cases with a positive HER2 status were significantly more common. Keywords: р53, HER2/neu, ERBB2, gastric cancer, gastric adenocar","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69281044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluation of hibernating myocardium function","authors":"M. Popov, D. Shumakov, L. Gurevich, D. Fedorov, D. Zybin, V. E. Ashevskaya, P. A. Korosteleva, V. Tyurina","doi":"10.31088/cem2023.12.1.59-67","DOIUrl":"https://doi.org/10.31088/cem2023.12.1.59-67","url":null,"abstract":"Introduction.Currently, there are different approaches to assessing changes that occur in ischemic myocardium in patients with chronic coronary artery disease (CAD). Researchers argue about the timing and completeness of the restoration of myocardial dysfunction areas. We aimed to assess hibernating myocardium in the zones of hypokinesia in patients with CAD. Materials and methods. We performed a morphological and immunohistochemical study of left ventricular myocardial biopsies of 25 patients who underwent surgical reconstruction of the left ventricle with surgical revascularization. Results. Morphological and immunohistochemical studies revealed violated morphological structure of cardiomyocytes. It correlates with the accumulation of MMP9 in the cytoplasm of cardiomyocytes in the areas of affected myocardium in ischemia against the background of partial or complete destruction of CM basement membranes formed by type IV collagen. It also correlates with long-term consequences of treatment. Conclusion. As a result of the destructed structure of sarcomeres and cardiac basement membrane hibernating myocardium is unable to provide a contractile function in the future. Morphological examination showed that viable cells were likely to function only as a protective mechanism in early scar formation. Keywords: left ventricular remodeling, hibernating myocardium, basement membrane, cardiomyocytes, matrix metalloproteinase 9, type IV collagen","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69281063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The content of neuroglobin in neurons of the parietal cortex and hippocampus of rats with cerebral ischemia of varying severity","authors":"E.I. Bon, N.E. Maksimovich, O.A. Karnyushko, V.F. Lazarev, S.M. Zimatkin, M.A. Nosovich, K.A. Khrapovitskaya","doi":"10.31088/cem2023.12.3.64-71","DOIUrl":"https://doi.org/10.31088/cem2023.12.3.64-71","url":null,"abstract":"Introduction. The role of neuroglobin ischemia is still unclear. Some studies indicate its neuroprotective effect due to increased expression of endothelial NOS. Other evidence refutes its significance for neuronal survival under oxygen-deficient conditions, as neuroglobin deficiency appears to increase HIF-1α expression. Materials and methods. The experiments were performed on 56 male outbred white rats weighing 258±18 g. Total cerebral ischemia was simulated by decapitation of animals, whereas the subtotal one was simulated by simultaneous ligation of both carotid arteries. Stepwise subtotal cerebral ischemia was performed by ligating both carotid arteries with an interval of 7 days (subgroup 1), 3 days (subgroup 2), or 1 day (subgroup 3). Results. The study found significant differences in neuroglobin content across three subgroups. In subgroup 1, there was a notable increase in neuroglobin content compared to the control group, with a 13% increase in the parietal cortex (p<0.05) and a 14% increase in the hippocampus (p<0.05). However, subgroup 2 showed a decrease in neuroglobin content, with a 13% decrease in the parietal cortex (p<0.05) and a 7% decrease in the hippocampus (p<0.05). The most significant decrease in neuroglobin content was observed in subgroup 3, with a 31% decrease (p<0.05) in the parietal cortex and a 33% decrease (p<0.05) in the hippocampus. In subgroup 3, the parietal cortex showed a 40% decrease in neuroglobin content compared to subgroup 1 (p<0.05) and a 21% decrease compared to subgroup 2 (p<0.05). Similarly, the hippocampus exhibited a 42.6% decrease in neuroglobin content compared to subgroup 1 (p<0.05) and a 28% decrease compared to subgroup 2 (p<0.05). Conclusion. Thus, the most pronounced disorders of the prooxidant-oxidant balance decreased neuroglobin were observed during a 1-day total cerebral ischemia. Keywords: neuroglobin, ischemia, pyramidal neurons, hippocampus, parietal cortex","PeriodicalId":36062,"journal":{"name":"Clinical and Experimental Morphology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135841723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}