Acta Seismologica Sinica最新文献

{"title":"ANTIBACTERIAL ACTIVITY AND MECHANISM OF GARDENIA JASMINOIDES ESSENTIAL OIL AGAINST STREPTOCOCCUS AGALACTIAE IN ACROSSOCHEILUS FASCIATUS","authors":"Shang-Ze SONG, Xian-Qi PENG, Lei Huang, Lingyun Lin, Yu CHEN, Huilin He, Liang CHEN, Yu-Long LIU, Ze-Ping LÜ, Jiayun Yao","doi":"10.3724/1000-3207.2025.2025.0320","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0320","url":null,"abstract":"This study aimed to investigate the antibacterial activity and underlying mechanism of <italic>Gardenia jasminoides</italic> essential oil (GEO) against <italic>Streptococcus agalactiae</italic> (GBS). GEO was extracted using supercritical CO₂ fluid extraction, and its chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). Results showed that GEO was primarily composed of alcoholic and olefinic compounds. <italic>In vitro</italic> assays revealed that the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GEO against GBS were 3.13 and 12.50 mg/mL, respectively. GEO at 1/2 MIC significantly inhibited bacterial growth, disrupted cell membrane integrity, and led to increased protein leakage and alkaline phosphatase activity (<italic>P</italic>&lt;0.05). Observations via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated that GEO at 1/2 MIC induced ultrastructural damage, including membrane rupture, cytoplasmic leakage, and vacuolization. Moreover, GEO at 1/2 MIC markedly suppressed biofilm formation and reduced the production of the quorum-sensing signal molecule autoinducer-2 (AI-2). <italic>In vivo</italic> challenge tests demonstrated that dietary supplementation with GEO (2 g/kg) significantly enhanced the survival rate of <italic>Acrossocheilus fasciatus</italic> upon GBS infection and notably reduced the bacterial load in the liver and brain tissues. These findings suggest that GEO possesses notable antibacterial activity against GBS, likely through the disruption of cellular structures, and exhibits potential for development as a green antibacterial agent in aquaculture.","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"50 4","pages":"042612-111"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciengine.com/doi/pdf/18FB9AC08FFD4C11A582E4835D2C1660","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIBACTERIAL AND PREVENTIVE EFFECTS OF TERMINALIA CHEBULA AND METHYL GALLATE AGAINST AEROMONAS HYDROPHILA ISOLATED FROM ALOSA SAPIDISSIMA","authors":"Hai-Jun TANG, Yao ZHENG, Yi Sun, Bingwen Xi, Kai Chen, Gangchun Xu","doi":"10.3724/1000-3207.2025.2025.0358","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0358","url":null,"abstract":"To explore effective approaches for preventing and controlling <italic>Aeromonas hydrophila</italic> infection in <italic>Alosa sapidissima</italic>, the pathogenic strain YML1 was first isolated from diseased fish and confirmed as an <italic>A. hydrophila</italic> resistant to β-lactam and tetracycline antibiotics. Antimicrobial susceptibility tests revealed that the water extract of <italic>Terminalia chebula</italic> and methyl gallate produced inhibition zone diameters of (21.00±0.75) and (24.00±1.50) mm, respectively, indicating high sensitivity. The minimum inhibitory concentration (MIC) were 5.00 mg/mL for <italic>T. chebula</italic> and 0.16 mg/mL for methyl gallate, while the minimum bactericidal concentration (MBC) was 40.00 mg/mL and 5.00 mg/mL respectively, demonstrating favorable in vitro antibacterial activity. Further investigation was conducted on the effects of dietary supplementation with <italic>T. chebula</italic> and methyl gallate on immune and antioxidant functions of <italic>A. sapidissima</italic> following challenge with <italic>A. hydrophila</italic>. The results showed that both treatments significantly improved survival rate, with <italic>T. chebula</italic> exhibiting more pronounced effects. Methyl gallate demonstrated potential as an immunostimulant, synergistically upregulating immune-related genes (<italic>lyz</italic>, <italic>tap2a</italic>, <italic>psmb9a</italic>) and antioxidant genes (<italic>gss</italic>, <italic>nrf1</italic>, <italic>cat</italic>), while simultaneously activating the complement system (C3, C4), lysozyme (LZM), and antioxidant enzyme activities (SOD, GSH-Px), thereby establishing a multi-level defense network. <italic>T. chebula</italic> enhanced basal immunity and pathogen recognition capacity of <italic>A. sapidissima</italic> through a multi-pathway, progressive mode by concurrently upregulating antioxidant genes (<italic>gss</italic>, <italic>cat</italic>), innate immune genes (<italic>lyz</italic>, <italic>tlr3</italic>), and antigen processing genes (<italic>tap2a</italic>), and significantly boosting their corresponding enzyme activities (T-SOD, GSH-Px, LZM, C3, C4). This approach effectively established robust defenses while avoiding excessive stress. In conclusion, both <italic>T. chebula</italic> and methyl gallate can effectively alleviate the stress and damage caused by <italic>A. hydrophila</italic> infection by modulating the immune-biochemical indicators and related gene expression in <italic>A. sapidissima</italic>, showing promise as potential feedadditives for bacterial disease prevention in this species.","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"50 4","pages":"042614-130"},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciengine.com/doi/pdf/91B6CBBB16584DAE8B4A5541B1FBE855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FUNCTIONAL STUDY OF 18-HEPE AND RVE1 IN LPS-INDUCED INFLAMMATORY RESPONSES OF HEAD KIDNEY MACROPHAGES IN LARGE YELLOW CROAKER","authors":"Ting Xu, Xiao Tang, Qinghui Ai","doi":"10.3724/1000-3207.2025.2025.0195","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0195","url":null,"abstract":"","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 11","pages":"102513-131"},"PeriodicalIF":0.0,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR CHARACTERIZATION AND TISSUE EXPRESSION OF THE &amp;lt;italic&amp;gt;BIM&amp;lt;/italic&amp;gt; GENE IN &amp;lt;italic&amp;gt;MEGALOBRAMA AMBLYCEPHALA&amp;lt;/italic&amp;gt; AND ITS RELATIONSHIP VERIFICATION WITH MAM-MIR-24-3","authors":"Si-Yu DING, Ya-Hui FENG, Kemmadzong Foning LOIC, Jie Chen, Guo‐Dong Zheng, Shu‐Ming Zou","doi":"10.3724/1000-3207.2025.2025.0077","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0077","url":null,"abstract":"To elucidate the structure and function of the <italic>bim</italic> gene in <italic>Megalobrama amblycephala</italic>, this study obtained the full-length coding sequence (CDS) of <italic>bim</italic> through TA cloning and systematically analyzed the primary, secondary, and tertiary structural characteristics of Bim protein by using bioinformatics tools. Multiple sequence alignment revealed that the Bim protein of <italic>Megalobrama amblycephala</italic> exhibited the highest homology (98.34%) with that of <italic>Chanodichthys erythropterus</italic>, and a Neighbor-Joining phylogenetic tree further supported their closest evolutionary relationship. Tissue expression profiling demonstrated that both the <italic>bim</italic> gene and its putative regulatory factor mam-miR-24-3 were predominantly expressed in gill tissues compared to other organs. After 24h of hypoxia stress, their expression levels in gills were significantly upregulated, suggesting potential synergistic roles in hypoxia adaptation. Dual-luciferase reporter assays confirmed that mam-miR-24-3 directly bound to the 3′-untranslated region (3′-UTR) of <italic>bim</italic> mRNA, leading to reduced luciferase activity. When combined with fluorescence co-localization analysis using green fluorescent protein, these results indicated that mam-miR-24-3 negatively regulated <italic>bim</italic> expression at the post-transcriptional level. Subcellular localization experiments revealed that Bim protein predominantly localized to the nucleus under both normoxic and hypoxic conditions, implying its potential involvement in nuclear signaling pathways to directly regulate downstream gene transcription. This study provides a theoretical foundation for elucidating the molecular mechanisms by which <italic>bim</italic> contributes to hypoxia adaptation in <italic>Megalobrama amblycephala</italic> and lays the groundwork for screening molecular targets in hypoxia-tolerant breeding strategies.","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 10","pages":"102511-113"},"PeriodicalIF":0.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciengine.com/doi/pdf/47D93DC7F571405A8D93D8AC256D68FA","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR CHARACTERISTICS AND FUNCTIONAL ANALYSIS OF ANTIOXIDANT-RELATED GENES &amp;lt;italic&amp;gt;NRF2, SIRT1,&amp;lt;/italic&amp;gt; AND &amp;lt;italic&amp;gt;OXR1&amp;lt;/italic&amp;gt; IN HYBRID PUFFERFISH (&amp;lt;italic&amp;gt;TAKIFUGU RUBRIPES&amp;lt;/italic&amp;gt; &amp;times; &amp;lt;italic&amp;gt;TAKIFUGU OBSCURUS&amp;lt;/italic&amp;gt;)","authors":"Yun-Long YAO, C. Wang, Yuan-Ye GAO, Shuo SHI, Mengmeng Wang, Zhe Zhao, Yan Shi","doi":"10.3724/1000-3207.2025.2024.0433","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2024.0433","url":null,"abstract":"<italic>Takifugu</italic>, commonly known as pufferfish, belongs to the family Tetraodontidae within the order Tetraodontiformes and is a warm-water marine benthic fish. This species is distributed along the coasts of China and in the western North Pacific. While primarily found in marine environments, some populations also inhabit freshwater. The fish is renowned for its rich, succulent skin, which contains spines and melts in the mouth, offering an aromatic and exquisite flavor, especially in wild specimens. However, with the increasing demand and production of pufferfish, intensive aquaculture practices have been associated with oxidative stress, leading to economic losses. To investigate the response mechanisms of hybrid pufferfish (<italic>Takifugu rubripes</italic> × <italic>Takifugu obscurus</italic>) to oxidative stress, this study cloned the cDNA sequences of key antioxidant genes <italic>Nrf2</italic>, <italic>SIRT1</italic>, and <italic>OXR1</italic>, followed by molecular characterization and functional analysis of these genes. Quantitative real-time PCR was employed to assess the tissue distribution of these genes. Results indicated that <italic>Nrf2</italic> was highly expressed in the liver, spleen, and muscle, while <italic>SIRT1</italic> exhibited high expression levels in the heart, liver, brain, and spleen. <italic>OXR1</italic> was predominantly expressed in the liver, brain, and eyes. Structural prediction analyses of the Nrf2 protein revealed a conserved Neh1 functional domain, suggesting its function may be similar across species. Further investigations using eukaryotic expression assays demonstrated that the transfection of <italic>Nrf2</italic> could induce the upregulation of the <italic>ho-1</italic> gene in cells. Under oxidative stress conditions, this led to enhanced cell viability and a reduction in reactive oxygen species levels, indicating that <italic>Nrf2</italic> possesses antioxidant functions. The findings of this study enhance the understanding of antioxidant genes in pufferfish and contribute to the elucidation of the physiological and biochemical mechanisms employed by these fish in response to oxidative stress.","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 9","pages":"092507-55"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREPARATION OF HIGHLY ACTIVE HeiCas9 PROTEIN AND ITS APPLICATION IN FISH GENE EDITING","authors":"Jiang-Yue LEI, Hou-Peng WANG, Kai Liu, Qian Wang, Ding Ye","doi":"10.3724/1000-3207.2025.2025.0041","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0041","url":null,"abstract":"To improve the efficiency of the CRISPR/Cas9 gene editing system, we designed and optimized Cas9 protein, and purified HeiCas9 protein with high activity was obtained by <italic>E. coli</italic> codon optimization, prokaryotic expression, immobilized metal affinity chromatography, molecular sieve chromatography, and ultrafiltration centrifugation concentration. In this study, HeiCas9 protein, Cas9 protein (T brand), and <italic>zcas9</italic> mRNA were combined with the gRNA of <italic>tyr</italic>, the key gene for melanin synthesis in zebrafish, to knockout <italic>tyr</italic> gene in zebrafish embryos at different concentration gradients, and the <italic>tyr</italic> gene editing efficiency was compared in different groups. At a working concentration of 400 ng/µL, HeiCas9 demonstrated significantly higher gene editing efficiency than T brand Cas9 protein while maintaining lower embryotoxicity. We further used HeiCas9 protein to knockout the genes <italic>dnd</italic> of <italic>Gobiocypris rarus</italic> and <italic>prkdc</italic> of <italic>Carassius auratus</italic>, with promising results. In summary, we have successfully generated highly active HeiCas9 protein, and validated its utility for efficient gene editing in multiple small fish embryos models under laboratory conditions.","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 9","pages":"092508-65"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DIFFERENT SOURCES OF DIETARY CHROMIUM ON GROWTH, IMMUNITY, AND GLUCOSE AND LIPID METABOLISM OF &amp;lt;italic&amp;gt;MACROBRACHIUM NIPPONENSE&amp;lt;/italic&amp;gt;","authors":"Ziming Zhao, Weixin Wu, Dong Zhou, Zihan Li, Youqin Kong","doi":"10.3724/1000-3207.2025.2025.0025","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2025.0025","url":null,"abstract":"This experiment was designed to explore the impacts of varying chromium sources on the growth, immunity, antioxidant capacity, and glucose metabolism of juvenile &lt;italic&gt;Macrobrachium nipponense&lt;/italic&gt;. The experimental design comprised four groups: a control group fed a basal diet devoid of chromium supplementation, and three treatment groups, each receiving a basal diet supplemented with 0.6 mg/kg of chromium in the forms of chromium chloride (CrCl&lt;sub&gt;3&lt;/sub&gt;), chromium-yeast (Cr-Yst), and chromium picolinate (Cr-Pic), respectively. Juvenile &lt;italic&gt;M. nipponense&lt;/italic&gt;, with an initial body weight of (0.120±0.023) g, were subjected to these dietary regimens over a period of 56d. The findings revealed that Chromium addition significantly increased the final weight (FW), weight gain (WG), specific growth rate (SGR), and protein efficiency (PER) of &lt;italic&gt;M. nipponense&lt;/italic&gt; compared to the control group (&lt;italic&gt;P&lt;/italic&gt;&lt;0.05). Moreover, prawns in the Cr-Yst and Cr-Pic groups exhibited significantly higher growth than those in the CrCl&lt;sub&gt;3&lt;/sub&gt; group (&lt;italic&gt;P&lt;/italic&gt;&lt;0.05). Conversely, the feed conversion ratios (FCR) of the prawns in Cr-Yst and Cr-Pic groups were the lowest (&lt;italic&gt;P&lt;/italic&gt;&lt;0.05). No statistically significant differences were observed in the survival rate across the groups (&lt;italic&gt;P&gt;&lt;/italic&gt;0.05). The addition of chromium also led to a significant reduction in the serum glucose (Glu) content of juvenile &lt;italic&gt;M. nipponense&lt;/italic&gt; (&lt;italic&gt;P&lt;&lt;/italic&gt;0.05). Furthermore, the ingestion of organic chromium significantly increased the activities of phosphatases (AKP, ACP) and concurrently decreased the activities of transaminases in the serum (ALT, AST) (&lt;italic&gt;P&lt;&lt;/italic&gt;0.05). Except for ACP, there were no significant differences in the activities of AKP, AST, and ALT between the CrCl&lt;sub&gt;3&lt;/sub&gt; group and the control group (&lt;italic&gt;P&gt;&lt;/italic&gt;0.05). Meanwhile, the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the hepatopancreas of the organic chromium groups were elevated compared to thosein the control group, whereas the malondialdehyde (MDA) content in the hepatopancreas was reduced (&lt;italic&gt;P&lt;&lt;/italic&gt;0.05). The supplementation of chromium was found to up-regulate the mRNA expression of glucose transporter 4 (&lt;italic&gt;glut4&lt;/italic&gt;), with the Cr-Yst and Cr-Pic groups exhibiting significantly higher levels than the control group (&lt;italic&gt;P&lt;&lt;/italic&gt;0.05), although no significant difference was observed to the CrCl&lt;sub&gt;3&lt;/sub&gt; group (&lt;italic&gt;P&gt;&lt;/italic&gt;0.05). Furthermore, dietary supplementation with chromium significantly increased the activity of key enzymes in the glycolytic pathway including hexokinase (HK) and phosphofructokinase (PFK), and diminished the activity of phosphoenolpyruvate carboxykinase (PEPCK) involving gluconeogenic pathway in the hepatopancreas of &lt;italic&gt;M. nipponense&lt;/italic&gt;. In comparison to the control group, the","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 9","pages":"092514-128"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciengine.com/doi/pdf/8DF3BDD2064C45EABBF41AC34CCE0B10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"氨氮和亚硝酸盐胁迫对大口黑鲈幼鱼抗氧化酶活性及相关基因表达的影响","authors":"Yanjie Yang, Jing Chen, Lei Huang, Xianqi Peng, Jin-Biao JIAO, Chen Niu, S H Yi, Jia-Yun YAO","doi":"10.3724/1000-3207.2025.2024.0355","DOIUrl":"https://doi.org/10.3724/1000-3207.2025.2024.0355","url":null,"abstract":"To investigate the effects of ammonia-N (NH&lt;sub&gt;3&lt;/sub&gt;-N) and nitrite (${rm{NO}}^-_2 $) on the antioxidant functions in largemouth bass (&lt;italic&gt;Micropterus salmoides&lt;/italic&gt;), acute stress experiments were carried out under controlled laboratory conditions in this study. The 96-hour lethal concentration (LC&lt;sub&gt;50&lt;/sub&gt;) for NH&lt;sub&gt;3&lt;/sub&gt;-N was determined to be 45.057 mg/L, with a safe concentration set at 4.506 mg/L. For ${rm{NO}}^-_2 $, the LC&lt;sub&gt;50&lt;/sub&gt; was 1365.514 mg/L, and the corresponding safe concentration was established at 136.551 mg/L. These safe concentrations were used as the maximum thresholds to evaluate the short-term exposure effects of NH&lt;sub&gt;3&lt;/sub&gt;-N and ${rm{NO}}^-_2 $ on the antioxidant enzyme activities and related gene expression levels in largemouth bass. The results showed that as the concentration of NH&lt;sub&gt;3&lt;/sub&gt;-N and ${rm{NO}}^-_2 $ increased, the weight gain rate of largemouth bass decreased. Additionally, under ${rm{NO}}^-_2 $ stress in isolation, malondialdehyde (MDA), glutathione (GSH), and total antioxidant capacity (T-AOC) activities decreased significantly. The relative expression levels of glutathione S-transferase (&lt;italic&gt;gst&lt;/italic&gt;) and glutathione peroxidase (&lt;italic&gt;gpx&lt;/italic&gt;) were significantly increased in gills, while superoxide dismutase (&lt;italic&gt;sod&lt;/italic&gt;) expression was significantly upregulated in low- and high-concentration groups in gills and in the spleen of the medium-concentration group. The relative expression of catalase (&lt;italic&gt;cat&lt;/italic&gt;) was significantly elevated in the spleen of the low- and high-concentration groups and in the gills of the high-concentration group. Under NH&lt;sub&gt;3&lt;/sub&gt;-N stress in isolation, the activity of total SOD (T-SOD) decreased in the high-concentration group, MDA activity decreased in the low-concentration group, and T-AOC and GSH activities decreased in the medium-concentration group. Under ammonia nitrogen stress alone, the relative expression levels of &lt;italic&gt;gst&lt;/italic&gt;, &lt;italic&gt;gpx&lt;/italic&gt;, &lt;italic&gt;sod&lt;/italic&gt; and &lt;italic&gt;cat&lt;/italic&gt; were significantly increased in the gills. Under dual stress conditions involving NH&lt;sub&gt;3&lt;/sub&gt;-N and ${rm{NO}}^-_2 $, MDA and T-SOD activities increased in the high-concentration group, while T-SOD, MDA, GSH, and T-AOC activities significantly decreased in the medium-concentration group. MDA activity decreased in the low-concentration group. Additionally, the expression levels of &lt;italic&gt;gst&lt;/italic&gt;, &lt;italic&gt;gpx&lt;/italic&gt;, &lt;italic&gt;sod&lt;/italic&gt;, and &lt;italic&gt;cat&lt;/italic&gt; were significantly elevated in liver tissue across all groups, as well as in the gills of the medium- and high-concentration groups. Consequently, acute stress caused by NH&lt;sub&gt;3&lt;/sub&gt;-N and ${rm{NO}}^-_2 $ induces oxidative stress in largemouth bass, and changes in antioxidant enzymes and antioxidant-related genes may play a role in scavenging reactive oxygen species, thereby mitigating potential damage caused by environmental fluctua","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"49 8","pages":"082506-1"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciengine.com/doi/pdfView/725B89A8138B462D9B22AA74FE7EEB6C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis Control of Al-Doped SrTiO₃ and Its Photocatalytic Performance for Overall Water Splitting","authors":"Yaling Luo, Zhanyang Zhuang, Fengtao Fan, Can Li","doi":"10.6023/a25050148","DOIUrl":"https://doi.org/10.6023/a25050148","url":null,"abstract":"","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"83 6","pages":"608-608"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sioc-journal.cn/Jwk_hxxb/EN/article/downloadArticleFile.do?attachType=PDF&id=351350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifunctional Electrocatalysts of Mn-doped Co₃O₄ for Oxygen Reduction and Oxygen Evolution Reactions in Alkaline Medium","authors":"Ziyi Shui, Sile Yu, Wei Lu, Liuyun Xu, Qingye Liu, Wei Zhao, Yilun Liu","doi":"10.6023/a24050152","DOIUrl":"https://doi.org/10.6023/a24050152","url":null,"abstract":"","PeriodicalId":35695,"journal":{"name":"Acta Seismologica Sinica","volume":"82 10","pages":"1039-1039"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}