Biomedicine (India)最新文献_第7页

Phytochemical analysis and antibacterial activity of traditional plants for the inhibition of DNA gyrase 传统植物抑制DNA旋切酶的植物化学分析及抑菌活性

Biomedicine (India) Pub Date : 2023-09-18 DOI: 10.51248/.v43i4.2859

Rajakumari K., Brindha Devi Parthiban, Shanmugapriya Rishikesan

{"title":"Phytochemical analysis and antibacterial activity of traditional plants for the inhibition of DNA gyrase","authors":"Rajakumari K., Brindha Devi Parthiban, Shanmugapriya Rishikesan","doi":"10.51248/.v43i4.2859","DOIUrl":"https://doi.org/10.51248/.v43i4.2859","url":null,"abstract":"Introduction and Aim: DNA gyrase is a class of Type II Topoisomerases that plays an important role in bacterial viability. It is found in all bacteria and is involved in replication, repair, recombination, and DNA transcription. Negative supercoiling of bacterial DNA by DNA gyr B is essential in replication which further influences all the metabolic activities. Staphylococcus aureus (ATCC 25923) is one of the pathogens that can modify its genome easily under multidrug resistance. In this study, the activity of medicinal compounds to inhibit DNA gyrB is explored. Plant species Solanum nigrum, Vitex negundo, and Euphorbia hirta were studied for the potential plant-based molecules. The compounds alkaloids, glycosides, flavonoids, and terpenoids were considered to have high-potential targets. The study focuses on DNA gyrase as a target and shows insights into future drug development. The research focuses on the discovery of novel plant-based therapeutic compounds to target DNA gyrase B activity. Methods and Materials: Phytochemical screening was performed to study the medication options that could inhibit DNA gyrB. Phytochemicals were determined using GC-MS. Results: Utilizing GC MS and FT-IR analysis, the phytochemical constituents of Solanum nigrum, Vitex negundo, and Euphorbia hirta were discovered. It will be simpler to do a follow-up study on discovering bioactive compounds and evaluating their effectiveness in inhibiting DNA gyrB with the help of this preliminary data from the analytical procedures. Conclusion: There are countless applications for the phytochemicals that medicinal plants produce. Staphylococcus aureus will be stopped by DNA gyrB inhibition. The study employs DNA gyrase as its target and provides information on potential therapeutic targets. The goal of the study is to identify innovative plant-based medicinal molecules that specifically target DNA gyrase B activity.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135208820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic utility of serum anti-Mullerian hormone levels in south Indian women with polycystic ovarian syndrome 南印度多囊卵巢综合征妇女血清抗苗勒管激素水平的诊断价值

Biomedicine (India) Pub Date : 2023-09-18 DOI: 10.51248/.v43i4.2312

Naveetha Lakshmi Narayanaswamy, Freethi Ramanathan, Moonishaa Thiyagarajan Manjuladevi

{"title":"Diagnostic utility of serum anti-Mullerian hormone levels in south Indian women with polycystic ovarian syndrome","authors":"Naveetha Lakshmi Narayanaswamy, Freethi Ramanathan, Moonishaa Thiyagarajan Manjuladevi","doi":"10.51248/.v43i4.2312","DOIUrl":"https://doi.org/10.51248/.v43i4.2312","url":null,"abstract":"Introduction and Aim: Polycystic ovarian syndrome (PCOS) is a prevalent endocrinopathy among women of reproductive age group, conventionally diagnosed using multiple clinical and biochemical tests. We aimed to evaluate the diagnostic utility of serum anti-Müllerian hormone (AMH) levels in PCOS. Materials and Methods: The case control study involved 60 women of 18-40 years age, further grouped using Rotterdam criteria as cases with PCOS (n = 30) and controls without PCOS (n = 30). Study variables were compared between the groups using independent t-test. Correlation analyses were performed to predict the relationship of AMH with other PCOS determinants. Simple logistic regression and ROC analyses were used to determine the diagnostic utility of AMH. Results: PCOS cases had increased levels of serum LH, testosterone, AMH, BMI, total antral follicular count (AFC), ovarian volume (OV) (p < 0.05*) than controls. Of all, serum AMH had the strongest association with PCOS (OR > 1, p < 0.05*) and showed considerable positive correlations with LH:FSH ratio, testosterone, AFC (r = 0.7768, p < 0.05*), OV (r = 0.7981, p < 0.05*). ROC analysis of AMH was significant (AUC = 0.966, p < 0.05*) with high sensitivity, specificity for cut points between 5.595 ng/mL and 5.90 ng/mL. Conclusion: Elevated serum AMH levels were strongly associated with PCOS and correlated with its routine clinical determinants. Serum AMH estimation with cut-off between 5.595 ng/mL to 5.90 ng/mL, is proposed as a useful index for PCOS diagnosis.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135208816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the molecular mechanisms underlying mutations in Mycobacterium tuberculosis RNA polymerase that confer resistance to rifampicin and its structural analogues 阐明结核分枝杆菌RNA聚合酶突变的分子机制，使其对利福平及其结构类似物产生耐药性

Biomedicine (India) Pub Date : 2023-09-18 DOI: 10.51248/.v43i4.3453

Varalakshmi Vummidi

{"title":"Elucidating the molecular mechanisms underlying mutations in Mycobacterium tuberculosis RNA polymerase that confer resistance to rifampicin and its structural analogues","authors":"Varalakshmi Vummidi","doi":"10.51248/.v43i4.3453","DOIUrl":"https://doi.org/10.51248/.v43i4.3453","url":null,"abstract":"Tuberculosis (TB) is a major global health problem caused by Mycobacterium tuberculosis (Mtb), and is responsible for significant morbidity and mortality worldwide. Rifampicin and its structural analogues are essential first-line anti-TB drugs that inhibit RNA synthesis by binding to the beta-subunit of Mtb RNA polymerase (RNAP). However, the emergence of rifampicin resistant Mtb strains poses a major challenge for TB control efforts. Mutations in the rpoB gene encoding the beta-subunit of RNAP are the most common cause of rifampicin resistance in Mtb. Understanding the molecular mechanisms underlying these mutations and their effects on RNAP function is crucial for developing new drugs and combination therapies to overcome rifampicin resistance in Mtb. This review discusses the molecular mechanisms underlying rifampicin resistance in Mtb RNAP, including the genetic basis and identification of mutations. It can be hypothesized that rifampicin resistance in Mtb RNAP is a multifactorial phenomenon involving structural, biochemical, and genetic factors. The review highlights strategies for developing new drugs and combination therapies to overcome rifampicin resistance in Mtb and future directions for research on the molecular mechanisms underlying rifampicin resistance in Mtb RNAP.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"177 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135208819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis 圆草通过诱导DNA断裂和凋亡对乳腺腺癌细胞的体外抗癌作用

Biomedicine (India) Pub Date : 2023-09-18 DOI: 10.51248/.v43i4.2309

Hema Nidugala, Ashwini Prabhu, Ramakrishna Avadhani, Ravishankar B.

{"title":"In vitro anticancer efficacy of Cyperus rotundus (L.) on breast adenocarcinoma cells via the induction of DNA fragmentation and apoptosis","authors":"Hema Nidugala, Ashwini Prabhu, Ramakrishna Avadhani, Ravishankar B.","doi":"10.51248/.v43i4.2309","DOIUrl":"https://doi.org/10.51248/.v43i4.2309","url":null,"abstract":"Introduction and Aim: Cyperus rotundus (L.), commonly known as nutgrass, has been in use in Indian medicine traditional system against several ailments. This investigation was performed to assess the in vitro antineoplastic activity of extracts prepared from water and ethanol with C. rotundus rhizomes. Materials and Methods: Toxic effects induced by the C. rotundus extracts on MCF-7 breast adenocarcinoma cells was evaluated using Sulphorhodamine- B (SRB) Assay, Trypan blue assay, Hoechst nuclear staining assay, Acridine Orange-Ethidium bromide (AO-EB) differential fluorescence staining assay and DNA fragmentation assays. Results: The results of SRB assay indicated the IC50 value of aqueous extract as 510.887 µg/mL and that of ethanol extract as 122.98 µg/mL. Trypan blue assay also indicated the similar results, wherein ethanol extract exerted higher cytotoxicity compared to the aqueous extract. MCF-7 cells treated with aqueous and ethanol extracts of C. rotundus rhizomes were exposed to Hoechst staining and AO-EB nuclear staining assays, wherein and ethanol extract induced clear apoptotic bodies in the tested cells. Further, the cells were treated with the ethanol extract and DNA fragmentation was confirmed by DNA ladder assay. MCF-7 cells administered with ethanol extract were subjected to DNA fragmentation assay for assessing the apoptosis induction nature of the extract. Conclusion: Results from our study indicate a promising approach into the possibility of developing C. rotundus ethanol extract into an effective anticancer moiety after appropriate pre-clinical and clinical validations.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"226 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135208817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exploring the therapeutic potential of green tea phytocompounds for pancreatic cancer: An mRNA differential gene expression and pathway analysis study 探索绿茶植物化合物对胰腺癌的治疗潜力:mRNA差异基因表达和途径分析研究

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.2869

Bhavana Sunkadakatte Venugopal, Susha Dinesh, Sameer Sharma, Srivarshini Govinda Srinivasan, Dinesh Sosalagere Manjegowda

{"title":"Exploring the therapeutic potential of green tea phytocompounds for pancreatic cancer: An mRNA differential gene expression and pathway analysis study","authors":"Bhavana Sunkadakatte Venugopal, Susha Dinesh, Sameer Sharma, Srivarshini Govinda Srinivasan, Dinesh Sosalagere Manjegowda","doi":"10.51248/.v43i4.2869","DOIUrl":"https://doi.org/10.51248/.v43i4.2869","url":null,"abstract":"Introduction and Aim: Pancreatic adenocarcinoma, which develops from the exocrine pancreas, is the most prevalent and aggressive type of pancreatic tumour having increased global prevalence, and has more than doubled over the 30 years. The aim of this study is to explore therapeutic potential of Camellia sinensis for pancreatic cancer using mRNA datasets and molecular docking technology. Materials and Methods: The purpose of the current study is to use computational methods to assess the effectiveness of several Camellia sinensis phytochemicals against Pancreatic cancer. The IMMPAT databank is utilized to retrieve the possible ligands. Molecular docking was methodically carried out using the virtual screening tool PyRx and the BIOVIA Discovery Studio Visualizer to forecast the binding affinity among the phytochemicals and the targeted proteins. Using ADMET filters, the ligands' pharmacological assessment was completed. Results: The fifty-two phytochemicals identified from Camellia sinensis were initially screened based on their affinity towards the targeted proteins. The ligand binding affinity score suggested that the phytochemical Vitamin E had the greater affinity towards the two targeted proteins and can be a promising therapeutic potential for the study of pancreatic cancer. Conclusion: The outcomes of this investigation suggested that the phytocompound Vitamin E, upon molecular docking exhibited the highest binding affinity which can be used as a drug candidate for pancreatic cancer.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136241291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of structural and functional alterations following traumatic brain injury in neurons and glial cells of the sensorimotor cortex 外伤性脑损伤后感觉运动皮层神经元和神经胶质细胞结构和功能改变的特征

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.3121

Elmira Mamytova, Nurbek Monolov, Bakytbek Turganbaev, Anara Toktomametova, Kunduz Karbozova, Aliya Kadyrova, Tamara Abaeva, Mira Zhanganaeva, Nursulu Chekirbaeva, Zhanara Altynbekova

{"title":"Characteristics of structural and functional alterations following traumatic brain injury in neurons and glial cells of the sensorimotor cortex","authors":"Elmira Mamytova, Nurbek Monolov, Bakytbek Turganbaev, Anara Toktomametova, Kunduz Karbozova, Aliya Kadyrova, Tamara Abaeva, Mira Zhanganaeva, Nursulu Chekirbaeva, Zhanara Altynbekova","doi":"10.51248/.v43i4.3121","DOIUrl":"https://doi.org/10.51248/.v43i4.3121","url":null,"abstract":"Introduction and Aim: Traumatic brain injury (TBI) is regarded as a significant worldwide health issue and a significant contributor to mortality and disability. The objective of this study was to examine the parameters and nature of the regenerative and destructive processes that occur in the rat cerebral cortex depending on the degree and time of the TBI. Materials and Methods: The experiments were carried out on 24 sexless adult mice weighing 180–220 g. The primary group of mice had severe TBI. To assess the severity of the TBI model, histological data, animal survival rates, and motor and cognitive dysfunctions were examined. Both light and electron microscopy were used to study the animal brains in each group. Results: The areas of injury were filled with blood, and microscopic examination revealed that the foci of contusion had destroyed brain tissue in the form of tissue and blood vessel fragments. Most dystrophic neuronal changes in mice with severe TBI between 1 and 21 days after the injury were acute neuronal swelling, hydropic dystrophy of nerve cells with clear cytoplasmic vacuolization, localized and complete chromatolysis, hyperchromatism, and homogeneous cytoplasm. Conclusion: Brain tissue lesions develop in the early stages of a TBI through rapid necrotic cell death.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136241552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic mutation rs972283 of the KLF14 gene and the incidence of gastric cancer KLF14基因突变rs972283与胃癌发生的关系

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.3112

Sabah Bresam, Rasha Majid Abd Ulameer Alhumairi, Istikrar M. Hade, Bahaa Abdullah Laftaah Al-Rubaii

{"title":"Genetic mutation rs972283 of the KLF14 gene and the incidence of gastric cancer","authors":"Sabah Bresam, Rasha Majid Abd Ulameer Alhumairi, Istikrar M. Hade, Bahaa Abdullah Laftaah Al-Rubaii","doi":"10.51248/.v43i4.3112","DOIUrl":"https://doi.org/10.51248/.v43i4.3112","url":null,"abstract":"Introduction and Aim: Genetic factors and family gene clustering constitute an important ratio for gastric cancer. Kruppel Like Factor 14 (KLF14) gene has a carcinogenic role and a clear role in metabolic diseases, but how this gene regulates these metabolic traits is still obscure. Previous studies proposed that the accumulation of single nucleotide polymorphisms (SNPs) in KLF14 may be associated with gastric cancer. The current study aimed to investigate whether single nucleotide polymorphisms (SNP) rs972283 in KLF14 is associated with an increased risk of gastric cancer in the Iraqi population.\u0000 \u0000Materials and Methods: The SNP was genotyped using tetra primer ARMS-PCR in 101 (79 men and 22 women) gastric cancer patients who did not receive chemotherapy, and 80 healthy controls (53 men and 27 women). All patient samples were taken from the Baghdad Hospital of Gastroenterology and Hepatology laboratories. Patient records included age, sex, histological type, and H. pylori infection status\u0000 \u0000Results: The KLF14 rs972283 genotype was significantly different between the gastric cancer and control groups. The heterozygous AG genotype and A mutant allele were significantly higher in gastric cancer patients compared to controls (56.4% vs 38.7%, p<0.01 and 61% vs 40.6%, p<0.01, respectively). In contrast, the GG wildtype genotype and G wildtype allele were significantly higher in controls (40% vs 11%, p<0.01 and 59.4% vs 39%, p<0.01, respectively). The AA homozygous mutant genotype also showed a weak correlation with increased gastric cancer risk. These results indicate the A allele is a risk factor while the G allele has a protective effect for gastric cancer.\u0000 \u0000Conclusion: the KLF14 polymorphism rs972283 exhibits a significant association with gastric cancer risk in our Iraqi cohort. The SNP may serve as a useful prognostic marker, pending validation in larger studies.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136241554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of fish on biochemical markers: A comparative study among male subjects of fish eaters and vegetarians 鱼类对生化指标的影响:男性食鱼者与素食者的比较研究

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.3345

Asha Prabhu, Kedilaya H. P., Manjula Shantaram

{"title":"Influence of fish on biochemical markers: A comparative study among male subjects of fish eaters and vegetarians","authors":"Asha Prabhu, Kedilaya H. P., Manjula Shantaram","doi":"10.51248/.v43i4.3345","DOIUrl":"https://doi.org/10.51248/.v43i4.3345","url":null,"abstract":"Introduction and Aim: Cardiovascular diseases (CVD) are the major causes of morbidity and mortality in India. Intake of a high or moderate amount of fish has shown a decreased risk of CVD. This is due to the presence of long-chain n-3 polyunsaturated fatty acids (PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oils. According to past studies, regularly consuming these fatty acids lowers the risk of heart failure, myocardial infarction, endothelial dysfunction, inflammation, arrhythmias, and sudden death. The beneficial effects of a fish-rich diet appear to conflict with PUFA's susceptibility to oxidation. As a result, the study aimed to compare the lipid profile, cardiac markers, and lipid peroxidation status in male adults who consume moderate amounts of fish regularly. Materials and Methods: Total cholesterol (T.C.), HDL-cholesterol (HDL-C), triglycerides (T.G.), high sensitive C-reactive protein (hs-CRP), Lipoprotein (a) [Lp(a)], Malondialdehyde (MDA) and antioxidant activity (AOA) were measured in healthy male individual of 85 fish eaters and 77 vegetarians in the age group of 25-40 years. The atherogenic indices TC/ HDL-C, LDL-C/HDL-C, a Student's 't' test, and non-HDL-C/ HDL-C were determined. The two groups' parameters were compared using the Student's t-test. Results: In Fish eating male subjects, the lipid profile and cardiac markers were decreased except for HDL-C (which was increased). Fish eaters exhibited significant decreases in atherogenic indices, and significant variation was not observed in the oxidant status of the study groups (p>0.05). Conclusion: According to the present study's findings, regular intake of moderate amounts of fish is linked to a decrease in lipid profiles and cardiac markers without affecting the individual's oxidation status.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136241556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of hepatic transaminases with cortisol levels in type 2 diabetes 2型糖尿病患者肝转氨酶与皮质醇水平的相关性

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.2734

Sujina S. S., Vinod Chandran, Poornima Manjrekar, Aradhana Marathe, Neelam Pawar

{"title":"Correlation of hepatic transaminases with cortisol levels in type 2 diabetes","authors":"Sujina S. S., Vinod Chandran, Poornima Manjrekar, Aradhana Marathe, Neelam Pawar","doi":"10.51248/.v43i4.2734","DOIUrl":"https://doi.org/10.51248/.v43i4.2734","url":null,"abstract":"Introduction and Aim: Aminotransferases are the markers of liver function and cortisol is the hyperglycemic stress hormone, both have been associated with insulin resistance and type 2 diabetes. Hypothalamic pituitary adrenal axis is known to control many functions of the liver. This study was aimed to evaluate the correlation of cortisol with hepatic transaminases; AST and ALT also with fasting blood glucose (FBG) and HbA1c in type 2 diabetic patients. Also, the AST, ALT and cortisol levels of participants were compared based on their diabetic duration and gender. Materials and Methods: This prospective study included patients with type 2 diabetes (n=89) with a mean diabetic duration of 7.66± 6.871 years, of which 66.3% were males and 33.7% were females. Data for the above parameters except cortisol were collected from the data management system of the central lab. Morning serum cortisol levels were estimated by enzyme-linked immunosorbent assay (ELISA) method. Karl Pearson’s correlation coefficient and independent student’s t-test were applied to find correlation and comparison of AST, ALT and cortisol levels of participants based on their diabetic duration and gender respectively. Results: The comparison based on diabetic duration shows significant differences (p<0.005) with AST while, not with ALT and cortisol. Also, they did not show a significant difference in either of the gender. Among hepatic transaminases, the probability of association of ALT with FBS levels and HbA1c levels was significant (p<0.05). However, though a positive trend is seen in the AST association, there is no strong correlation observed. Likewise, the association of serum cortisol levels, with AST, ALT and HbA1c was not significant but the probability of association was significant with FBS levels. Conclusion: Findings from the present study suggest that liver transaminases are positively correlated with serum cortisol levels in type 2 diabetic patients.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136240805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The antagonistic effect of Anabaena circinalis on some dermatophytes 鱼腥鱼对几种皮肤植物的拮抗作用

Biomedicine (India) Pub Date : 2023-08-30 DOI: 10.51248/.v43i4.3026

Zainulabdeen H. A. Al-Khafaji

{"title":"The antagonistic effect of Anabaena circinalis on some dermatophytes","authors":"Zainulabdeen H. A. Al-Khafaji","doi":"10.51248/.v43i4.3026","DOIUrl":"https://doi.org/10.51248/.v43i4.3026","url":null,"abstract":"Introduction and Aim: Anabaena circinalis, a photosynthetic cyanobacterium belonging to the Gram-negative group, is widely distributed in freshwater ecosystems across the world. The scientific focus on A. circinalis primarily stems from its ability to produce numerous cyanotoxins, which have the potential to be hazardous. The objective of this study was to assess the antifungal efficacy of A. circinalis against the dermatophytes Trichophyton rubrum and Trichophyton mentagrophytes, with the aim of discovering a new antifungal agent. Methodology: The identification of Anabaena circinalis in this study was conducted through the observation of its morphological characteristics. Molecular identification and confirmation of the algae was done using the polymerase chain reaction (PCR) method, employing a specific set of primers targeting the PCbetaand PCalpha genes. Gas chromatography-mass spectrometry was employed in identifying A. circinalis antifungal compounds. The ability of 70% ethanolic and methanolic extracts produced from A. circinalis to inhibit the dermatophyte fungi Trichophyton rubrum and Trichophyton mentagrophytes was tested by in vitro tests. Results: The findings indicate that the hot ethanolic extract of Anabaena circinalis exhibited a significant inhibitory effect (100% inhibition) on the growth of dermatophyte fungi tested. Gas chromatography-mass spectrometry studies identified several antifungal compounds in A. circinalis extracts, which included Hexadecen-1-ol, Phthalic acid, Octadecenoic acid, Octadecynoic acid, Hexadecanoic acid, Pentadecadien, Tetradecenal, and Octadecadien-1-ol. Conclusion: Overall finding suggests hot ethanolic or methanolic extracts of Anabaena circinalis contain phyto components, which could be used as an antifungal agent in treating dermatophyte fungi-related infections.","PeriodicalId":35655,"journal":{"name":"Biomedicine (India)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136241292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1