Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology最新文献

{"title":"Determination of doxorubicin amount conjugated to mPEG-b-PCL copolymer via pH sensitive hydrazone bond","authors":"Gülhan Işik, A. Tezcaner, N. Hasirci, A. Kiziltay","doi":"10.5505/turkhijyen.2022.04317","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.04317","url":null,"abstract":"Objective: The aim of this study was to find the efficient medium to bind an anticancer drug, Doxorubicin (DOX), to a synthesized polymer, methoxy poly(ethylene glycol)-block-polycaprolactone (mPEG-b-PCL) via pH sensitive hydrazone bonds and to determine the amount of conjugated drug. Methods: DOX conjugation was carried out in two different media [dimethyl sulfoxide (DMSO) and methanol-trifluoroacetic acid (MeOH-TFA)]. The amount of conjugated drug was determined with two different methods. One method was applied the dissolution of the conjugate in chloroform: methanol (Ch: MeOH, 1: 1 v/v) solution without considering pH responsiveness, and the other method was after breaking pH sensitive hydrazone bonds in acidic medium [using three different media as 0.1 M hydrochloric acid (HCl), concentrated HCl (12 M HCl) and concentrated sulfuric acid (18.3 M H2SO4)]. Results: The highest conjugation efficiency was obtained when the conjugation was achieved in MeOH-TFA solution, and for the polymer-drug conjugates after the treatment with concentrated sulfuric acid. recognized by immune system rapidly (12). On the other hand, PEG is hydrophilic and can escape from the immune system. Scientists prepared or used copolymers or tri-block-polymers of PEG and PCL and prepared either nanoparticles or micelles as drug delivery vehicles and evaluated in vitro and in vivo properties (13). Gong et al studied affinity of PEG-PCL-PEG micelles (14). Hu et al prepared micelles and polymersomes from PCL-PEG-PCL polymers to be used as drug carriers (15). Cytotoxic properties of Doxorubicin loaded PEG-PCL micelles prepared with functionalized PEGs were evaluated by Chen et al. and promising results were reported (16). An accurate determination of drug conjugation is important since higher dose given to a patient may create some unwanted symptoms like cardiomyopathy. In this study, methoxy konjugasyonu Conclusion: It was concluded that, MeOH-TFA method was a good method for conjugation of DOX to M) method was better other present in literature for determination of the amount of DOX linked to the polymer via","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cross-protection of Androctonus crassicauda antivenom against Leiurus abdullahbayrami venom.","authors":"Mehmet Ali Kanat, D. Altun, Kübra Kiliç, Edibe Nurzen Namlı Bozkurt, Ertuğrul Turan, G. Cengi̇z, İlhan Bozyi̇ği̇t","doi":"10.5505/turkhijyen.2022.34445","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.34445","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella Typhimurium and Salmonella Hessarek in wild birds","authors":"E. Günaydın, Özlem Kardoğan, G. Goncagül, Yavuz Çokal, Pınar Mursaloğlu Kaynar","doi":"10.5505/turkhijyen.2022.34538","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.34538","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autacoids in the inflammation","authors":"S. Kemer, Sefa Meti̇n, Gökçe Sural, E. Demirel Yilmaz","doi":"10.5505/turkhijyen.2022.66742","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.66742","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Molecular Mechanisms in Targeted Therapy Strategies in Ovarian Cancer","authors":"B. Çolak, D. Cansaran Duman, E. Yilmaz","doi":"10.5505/turkhijyen.2022.60362","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.60362","url":null,"abstract":"Cancer is a disease that occurs in tissues and organs as a result of uncontrolled cell divisions. There are numerous types of cancer related with different frequencies and mortality rate. Ovarian cancer is the seventh common type of cancer worldwide and is the fifth cause of cancer-related death. Although there are standard treatment methods for cancer types, people give different responses to these treatment methods. In spite of all the treatment strategy developed, ovarian cancer still has a high mortality rate. For this reason, it is very important to better understand the drug resistance mechanisms that prevent the effecttiveness of today’s treatment methods, to develop new treatment methods and the determination of molecular characterization. In recent years, research has focused on methods containing pathway analysis within the scope of developing targeted therapy strategies for ovarian cancer. In this context, the activity status of onco-proteins in pathways such as WNT, Hedgehog and NOTCH signaling pathways were evaluated. In addition, the innovative treatment efficiency of Nanog protein and photodynamic therapy options on ovarian cancer has been detailed in recent years. It will be possible to increase the quality of the healing process of ÖZET Kanser, kontrol edilemeyen hücre bölünmeleri sonucunda doku ve organlarda meydana gelen hasarların bütünüdür. Farklı görülme sıklığı ve ölüm oranları ile ilişkili birçok kanser türü vardır. Yumurtalık kanseri dünya genelinde yaygınlık olarak yedinci sırada gelmekte olup kansere bağlı ölüm nedeni olarak beşinci sırada gelmektedir. Kanser türlerinin kendi içinde standart tedavi yöntemleri olmasına rağmen bireyler bu tedavi yöntemlerine farklı cevaplar vermektedir. Geliştirilen tüm tedavi stratejilerine rağmen yumurtalık kanseri hala yüksek ölüm oranına sahiptir. Bu sebeple günümüzdeki tedavi yöntemlerinin etkinliğini engelleyen ilaca karşı oluşan direnç mekanizmalarını kavramak, yeni tedavi yöntemleri geliştirmek ve moleküler karakterizasyonunu belirlemek oldukça önemlidir. Son yıllarda oldukça gündeme gelen yumurtalık kanserinde hedefe yönelik terapi stratejileri geliştirilmesi kapsamında yolak analizleri üzerine odaklanılmıştır. Bu kapsamda onkoproteinlerin WNT, Hedgehog ve NOTCH sinyal yolağı gibi yolaklardaki etkinlik durumları değerlendirilmiştir. Ayrıca, son yıllarda etkinliği belirlenmiş Nanog proteini ve fotodinamik tedavi seçeneklerinin yumurtalık kanseri üzerindeki yenilikçi tedavi etkinliği detaylandırılmıştır. Yumurtalık kanseri tedavisinde mevcut ve geliştirilmesi Türk Hijyen ve Deneysel Biyoloji Dergisi 1Ankara Üniversitesi Biyoteknoloji Enstitüsü, Ankara Geliş Tarihi / Received : Kabul Tarihi / Accepted : İletişim / Corresponding Author : Demet CANSARAN DUMAN Ankara Üniversitesi, Biyoteknoloji Enstitüsü Merkez Laboratuvarı, Tandoğan / Ankara Türkiye E-posta / E-mail : dcansaran@yahoo.com 24.02.2021 16.04.2021 Çolak B, Cansaran Duman D, Yılmaz E. Yumurtalık kanserinde hedefe yönelik te","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70677085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Certified Health Education Programs Under the Coordination of The Türkiye Ministry of Health by The Participants","authors":"Gülsen Topaktaş, Emine Çetin Aslan, Hüseyin Aslan","doi":"10.5505/turkhijyen.2022.02256","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.02256","url":null,"abstract":"","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of tDCS on Depression and Anxiety Disorders Induced by Sub-Chronic Stress","authors":"G. Akcay, N. Derin","doi":"10.5505/turkhijyen.2022.06641","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.06641","url":null,"abstract":"Objective: Stress causes neuropsychiatric diseases such as depression and anxiety in the person by affecting emotions, opinions and behaviors. In this study, the aim was to investigate the therapeutic effects of tDCS on depression and anxiety disorders that occur after sub-chronic stress. Methods: 40 male Wistar rats weighing 290-310 g were divided into four groups as control, tDCS, stress and stress+tDCS. Restraint stress was applied for 7 days (2 h/day) as a stress model. Rats in the tDCS and stress+tDCS groups were performed the stimulation of 1 mA anodal tDCS for three consecutive days from the 5th day of stress exposure. On the 7th day of the experiment, the anxiety and depression behaviors of the rats were evaluated ANKSIYETE BOZUKLUKLARI indicator of anxiety (p<0.001). In the stress + tDCS group compared to the control group, there was a significant decrease in the data indicator of anxiety following tDCS application (p <0.05), but no significant difference was found in the results of locomotor activity. Conclusion: In conclusion, our data showed that tDCS stimulation may have an effect on sub-chronic stress-induced anxiety and","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of fungi for the production of biotechnological products in the field of health","authors":"Şule Aybüke Yavuz, Ü. Gül","doi":"10.5505/turkhijyen.2022.06982","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.06982","url":null,"abstract":"Biotechnology is an interdisciplinary science that aims to produce products of high economic value using biological materials. Fungi are used as a source in many industrial processes such as the production of enzymes, vitamins, polysaccharides, polyhydric alcohols, pigments, lipids and glycolipids. These products are potentially valuable in terms of biotechnological studies as well as the economic value provided by their commercial production. The first biotechnological products include alcoholic beverages such as wine and beer also, basic nutrients such as bread and cheese produced by the use of yeast and fungi. Especially with the discovery of the first antibiotic, the possibilities of using fungi for the production of drugs and similar substances in health biotechnology have been started to be investigated. Fungal secondary metabolites are extremely important for our health and nutrition and also have a significant economic impact. In recent years, modified organisms have been used in biotechnological production as a result of the use of techniques developed by molecular biology and genetic studies. Today, there has been a significant increase in production and marketing activities for microbial enzymes and pharmaceutical üretimi ürünler ticari üretimleriyle mantarlar, sağlık products produced by recombinant DNA technology, including yeasts and other fungi as hosts. Today, fungal biology is an important participant in the global industry. Genomic and proteomic studies on newly discovered species are ongoing. The aim of this study is to examine the information in the literature about the use of fungi in health biotechnology. According to the results of this study, yeasts and fungi are widely used in health biotechnology, especially in the production of pharmaceutical products. Fungi and yeasts are used in the production of various pharmaceutical products such as antitumor and antimicrobial drug active substances, vaccines and hormones. In addition, it has been determined that new fungi and yeast species developed using recombinant DNA technologies can be used both more efficiently and effectively. In addition, the existence of newly isolated yeast and fungi species with many features not fully identified is promising for the production of new products in","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70675625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of gram negative bacteria isolated from blood culture and antibiotic resistance rates","authors":"Nihan Çeken, Hülya Duran, Tuğba Kula Atik","doi":"10.5505/turkhijyen.2022.57983","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.57983","url":null,"abstract":"Objective: Bacteremia is a serious condition that causes hospitalization and increases mortality. Gram negative bacteria are frequently isolated as causative agents. The aim of this study was to evaluate the distribution and antimicrobial resistance of the gram negative bacteria isolated from blood cultures in our hospital. Methods: Gram negative bacteria isolated from blood culture samples sent to the microbiology laboratory between 2016-2019 were retrospectively analyzed. The blood cultures were performed by BacT/ALERT 3D (bioMérieux, France) and Render-BC128 (China). Bacterial identification and antibiotic susceptibility tests were performed using conventional methods and BD Phoenix 100 (BD Phoenix System, Beckton Dickinson, ABD) automated systems. Results: A total of 10,315 blood cultures obtained from hospitalized patients between (95.4%), Pseudomonas aeruginosa to ciprofloxacin (26.1%). The most susceptible antibiotics were carbapenems and amikasin for Enterobacterales , amikasin and gentamycin for P. aeruginosa , amikasin and trimethoprim-sulfamethaxazole for A.baumannii . Conclusion: In our study, the frequency of gram-negative bacteria growth in blood culture was found to be 27.5%, similar to literature data. It was observed that the determined bacterial distribution was compatible with different studies. Blood culture is the most important test in the diagnosis of bacteremia and in directing the right treatment. The distribution of microorganisms isolated in bacteremia and their antibiotic resistance rates changes by center. For this reason, it was think that each hospital should determine its own data at regular intervals and guide antibiotic selection according to these results.","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of diabetes mellitus in the progression and prognosis of COVID-19","authors":"Filiz Yıldırım, H. Karageçili, Reyhan Öztürk, Zuhal Yıldırım","doi":"10.5505/turkhijyen.2022.58708","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.58708","url":null,"abstract":"Objective: The COVID-19 pandemic, caused by SARSCoV-2 of Coronaviruses types, is a highly infectious disease caused by SARS-CoV-2, which first appeared in China. The presence of comorbid diseases, especially diabetes, and advanced age are determinants of the mortality and morbidity of the disease. In this study, it was aimed to examine the possible role of Diabetes mellitus (DM) in the course of the novel coronavirus disease (COVID-19). Methods: In this study, the data of 81 patients who applied to Ankara Polatlı Duatepe State Hospital between January 01 and May 05, 2021 and were confirmed to have COVID-19 and were hospitalized in the COVID-19 service for 5 to 20 days were analyzed. 39 female and 42 male patients were included in the study and the patients were divided into three groups. COVID-19 group (n=26;10 female, 16 male), COVID-19+DM group (n=28;13 female, 15 male), and COVID-19+hypertension (HT) group (n=27;16 female, 11 male). Demographic, clinical, radiological and laboratory records of the patients were reviewed retrospectively. Results: There was a statistically significant difference between the groups when they matched for age and gender (p<0.05). The mean age of the COVID-19+DM and COVID-19+HT groups was higher than the COVID-19 group (p<0.05). COVID-19 group 55.96±15.545 years, COVID-19+DM 68.29±12.849 years, COVID-19+HT 71.48±11.416 years. Only 32 patients had positive PCR tests, and the rate was 39.5%. The number of patients with CT is 69, and the rate is 85.2%. The number of CT-positive patients is 56 and the positivity rate is 81%. The PCR test positivity rate is lower than the CT positivity rate. The serum fasting blood glucose (FBG) and C-reactive protein (CRP) levels were significantly higher in the COVID-19+DM group when compared to the COVID-19 group and COVID-19+HT group (p<0.05). The serum sodium (Na) and chlorine (Cl) levels were significantly lower in the COVID-19+DM group when compared to the COVID-19 group and COVID-19+HT group (p<0.05). The serum creatinine and phosphorus (P) levels were significantly higher in the COVID-19+DM group when compared to the COVID-19 group (p<0.05).The serum hemoglobin (HGB) and hematocrit (HCT) levels were considerably higher in the COVID-19 group compared to the COVID-19+DM group (p<0.05). Conclusion: When all the data we obtained in our study are evaluated;we determined that the comorbidity effect of DM is important in the clinical course of patients infected with the SARS-CoV-2 virus. We think that SARSCoV-2 pneumonia patients with diabetes may be more severe than those without diabetes in terms of organ damage, and inflammatory variables, and are more likely to evolve to a worse prognosis, regardless of whether the additional comorbidities were present or not. © 2022,Turk Hijyen ve Deneysel Biyoloji Dergisi. All Rights Reserved.","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70677146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}