{"title":"Activation of apoptotic cell death by skin mucus from Asian swamp eel (Monopterus albus) against human lung cancer cell line","authors":"A. Hilles, S. Mahmood, M. Kaderi, R. Hashim","doi":"10.24200/jams.vol24iss0pp39-43","DOIUrl":"https://doi.org/10.24200/jams.vol24iss0pp39-43","url":null,"abstract":"Asian swamp eel (Monopterus albus) is a freshwater fish which distributed widely in the East of India mainly across Malay Peninsula and Indochinese Peninsula, it is also broadly distributed in the Southern areas of East Asia including, southeastern China, Western Japanese Archipelago, and Korean Peninsula. It lives in muddy places, rice paddies, and slow-flowing currents areas. It has a unique morphological elongated body which is similar to snake and covered with a thick layer of the mucus. The objective of this study is to screen the cytotoxic activity of eel skin mucus extracts and to evaluate the mechanism of cell death whether it is apoptosis or necrosis based on caspases activity assays. For this purpose; eel skin mucus was collected from healthy fresh eels to prepare aqueous and methanol extracts. Preliminary cytotoxicity study was demonstrated against Non-small-cell lung carcinoma cell line (A549) using cell viability assay which revealed that methanol extract is more potent than aqueous extract; the revealed data was based on the response of ESM methanol and aqueous extracts to the relevant IC50 concentrations as IC50 values were 621±0.09 µg/mL and 845 ± 0.25 µg/mL respectively. Subsequently, the mechanism of cell death induced cell death through triggering apoptosis by the activation of caspase-3/7, 8 and 9. In conclusion, the present study has revealed the cytotoxic potentials of eel skin mucus which may lead to the development of new anticancer agents. ","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131673941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Alsaffar, Abdulrahman Bin Hassan, T. Dashti, Ali Taqi, A. Al‐Mousawy, A. Alalawi
{"title":"The importance of the intertidal area for the Mullet Liza klunzingeri","authors":"A. Alsaffar, Abdulrahman Bin Hassan, T. Dashti, Ali Taqi, A. Al‐Mousawy, A. Alalawi","doi":"10.24200/JAMS.VOL23ISS0PP68-75","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP68-75","url":null,"abstract":"The mullet Liza klunzingeri, locally known as maid, is an important commercial species in Kuwait and accounts for up to 16% of the total finfish landings. To determine the seasonal and spatial distributions of juvenile maid, a small-mesh seine net was employed monthly at seven selected coastal sites during the period from November 2013 through October 2014. Semicircular sweeps of the 30 m long, two-meters high seine covered an area of approximately 1,400 m2. Juvenile maid were captured at most of the sampling sites during the entire investigation period, but mainly in spring and summer, indicating a wide coastal distribution of this species. The highest catches of juveniles occurred in May with an average of 30.2 kg per sweep, corresponding to a density of 22 g/m2. Catches of juveniles from sampling sites in or just south of the Kuwait Bay, near prime maid fishing grounds, were much higher than those from more southerly sampling sites. The captured maid ranged in total length (TL) from 18 to 179 mm, with a high peak at 41 to 50 mm. By-catch included thirty species, with hasoom (Sillago sihama), Imchawah (Diplodus sargus kotschyi), and yowaff (Nematalosa nasus) being the most common. The by-catch consisted mostly of juveniles with lengths less than100 mm TL, showing the importance of the intertidal and coastal waters for many species.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126154133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Overview of Decadal Ecosystem Changes in the Western Arabian Sea and the Occurrence of Algal Blooms","authors":"P. Harrison, S. Piontkovski, K. Al-Hashmi","doi":"10.24200/JAMS.VOL23ISS0PP11-23","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP11-23","url":null,"abstract":"Gradual decadal changes have taken place in the Western Arabian Sea over the last 50 years. These changes have affected wind speeds, atmospheric and sea surface temperature, thermohaline stratification, shoaling of the oxycline, and dust/iron inputs. A decrease in nitrate supply of the photic layer have caused an increase in annual frequency of harmful algal blooms and fish kills. Along with that, a decrease in diatom biomass and a shift from red Noctiluca to green Noctiluca during the northeast monsoon was observed during the last two decades, Even though these are the same species they have very different nutritional modes. The red one is a heterotroph with a preference for grazing diatoms, while the green one has a symbiont and thus it is a mixotroph. Recent results suggest that this shift may be caused by the shoaling oxycline since the green one grows better under low oxygen because the symbiont produces oxygen for its host. The western Arabian Sea is temporally and spatially complex. With the recent advances in remote sensing of the ocean, a further understanding of these temporal and spatial changes can be gained through analyzing frequent images with opportunistic ground-truthing.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123796380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. A. Al-Qartoubi, S. Bose, H. Al-Masroori, A. Govender
{"title":"Circle hook versus J-hook: A case study of the Sultanate of Oman","authors":"I. A. Al-Qartoubi, S. Bose, H. Al-Masroori, A. Govender","doi":"10.24200/JAMS.VOL23ISS0PP29-39","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP29-39","url":null,"abstract":"This paper analyses the operational efficiency and economic performance of the circle hook and the J-hook, commonly used by traditional fishers in the demersal longline fishery of the Sultanate of Oman. A longline experiment was conducted at three fishing locations at Masirah Island of Al-Sharqiyah Governorate. A total of 6,120 baited J-hooks and circle hooks were deployed over a 17-day period. The findings from this experimental research suggest that the overall performance (measured under various operational yardsticks such as hooking status and location, catch composition and quality, catching efficiency, and time and cost efficiency) of the circle hook is better than its counterpart. The results also indicate that the use of circle hooks has the potential to yield better financial returns. It is acknowledged that the reliance on three fishing locations may restrict the scientific generalizations. However, it is hoped that the results from this study will provide insight into the design of future experiments to ensure the validity of the present results and make effective regulatory decisions.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125740561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"2nd GCC Marine Biotechnology conference: emerging opportunities and future perspectives","authors":"S. Dobretsov","doi":"10.24200/JAMS.VOL23ISS0PP48-51","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP48-51","url":null,"abstract":"2nd GCC Marine biotechnology conference was held at Sultan Qaboos University, 31 October – 1 November 2017. The conference was organized by the Center of Excellence in Marine Biotechnology, SQU. The conference focused on four major themes, including Genetics and biodiversity, Marine biofouling and its prevention, Marine natural products, and Aquaculture and fisheries. In total, 160 delegates from 23 different countries were registered for this conference and 106 delegates attended the conference. One plenary lecture and three keynote addresses were given, 39 oral and 30 poster presentations were made. The final session reviewed national and regional issues and identified some of the main challenges in marine biotechnology in the region.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"134 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120940729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laila Al-Naamani, T. Muthukrishnan, J. Dutta, S. Dobretsov
{"title":"Antifouling properties of chitosan coatings on plastic substrates","authors":"Laila Al-Naamani, T. Muthukrishnan, J. Dutta, S. Dobretsov","doi":"10.24200/JAMS.VOL23ISS0PP92-98","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP92-98","url":null,"abstract":"In the current study, chitosan coatings were fabricated on plastic substrata and investigated for their antifouling activities. Scanning Electron Microscopy (SEM) and water contact angle measurement (WCA) of the fabricated chitosan films showed smooth and hydrophilic surface with WCA below 60°. Chitosan coating on plastic substrate showed 88% reduction in settlement of bryozoan Bugula neritina larvae compare to the control after 3 hours incubation in dark conditions with no larval mortality during the experiment. The antimicrobial activity of chitosan was evaluated by coating of plastic panels with the prepared chitosan solution and immersing the coated samples in seawater with controlled environmental conditions. Biofilms scraped from immersed chitosan coated panels showed no bacteria after 1 week immersion. After the second week of immersion, less than 1500 bacteria/mm2 was observed on the chitosan-coated panels compared to more than 105 bacteria/mm2 on uncoated ones. Thus, this study prove the efficiency of chitosan coatings against micro- and macro-fouling.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124004636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integrated management of the Fusarium vascular wilt disease of Cucurbita pepo in Iraq","authors":"S. Hussein","doi":"10.24200/jams.vol23iss0pp40-47","DOIUrl":"https://doi.org/10.24200/jams.vol23iss0pp40-47","url":null,"abstract":"Fusarium vascular wilt disease is one of the most harmful disease that affected broad range of plant species including zucchini (Cucurbita pepo). The objective of the research was to investigate the presence of the phytopathogenic gungus Fusarium oxysporum the causal agent of the disease in zucchini fields. Forty five isolates of F. oxysporum were isolated from four locations in province of Dyala during 2014-2015. Isolate Foq9 was most virulent in the pathogenicity test in vitro. Twenty nine isolates of them amplified their DNA positively with the universal primer of F. oxysporum in the polymerase chain reaction technique (PCR). In vitro significant suppression efficiency were observed of the fungicides Topsin M 70 WP (Tm) and Tecto 500 SC (Tc) and two botanical extract solution extracted from Garlic (Gr) and Ginger (Gn) against the pathogen on the potato sucrose agar (PSA). In greenhouse experiments all of the agents decreased the percentage of disease incidence and severity significantly, while the tetra-inoculum (Tm+Tc+Gr+Gn) was superior which exhibited 0% disease incidence and severity compared to the negative control which was 95%, 79% respectively, also the combined application of the botanical extracts (Gr+Gn) reduced the disease incidence and severity significantly. All of the treatment increased plant growth criteria represented by dry weight of the plant compared to the control.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123741205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buthaina Al Khaziri, A. A. Sulimani, Najla Al Mandhari, G. Yoon, Azhar Al-Busaidi
{"title":"Bacterial Infection in Farmed Barramundi Juveniles, Lates calcarifer","authors":"Buthaina Al Khaziri, A. A. Sulimani, Najla Al Mandhari, G. Yoon, Azhar Al-Busaidi","doi":"10.24200/JAMS.VOL23ISS0PP76-80","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP76-80","url":null,"abstract":"In 2016, Barramundi, Lates calcaifer juveniles imported from Thailand to the aquaculture station at Sultan Qaboos University exhibited remarkable external clinical signs of losing scales, severe muscle necrosis, and ascites. Fin rot was also observed in the diseased fish. The mortality reached more than 90 % and it was assumed that certain bacterial species were associated with the diseased fish. The objective of the present study was to investigate the infection route of disease, revealing the causative agent and finding the most effective antibiotic treatment. The suspected pathogen vehicle was mosquito larvae in the outlet of the culture tank as bacterium was isolated inside the mosquito larvae. Primary, traditional phenotypic tests and the vitic test confirmed that the bacteria were pathogenic Aeromonas sobria and Lactococcus garvieae. Eight of the most commonly used antibiotics in the aquaculture industry was used for antibiotic susceptibility test. It showed that that Gentamycin was the most effective antibiotic while the most effective environmentally friendly source was henna, Lawsonia inermis, at a concentration of 10%.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117074519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Q. Al-MAWAALI, A. Al-Sadi, F. Al-Said, Mohammed Shafiur Rahman, I. Al-Zakwani, Amanat Ali, M. Al-Yahyai, M. Deadman
{"title":"Effect of grafting on resistance to vine decline disease, yield and fruit quality in muskmelon cv. Sawadi","authors":"Q. Al-MAWAALI, A. Al-Sadi, F. Al-Said, Mohammed Shafiur Rahman, I. Al-Zakwani, Amanat Ali, M. Al-Yahyai, M. Deadman","doi":"10.24200/JAMS.VOL23ISS0PP2-10","DOIUrl":"https://doi.org/10.24200/JAMS.VOL23ISS0PP2-10","url":null,"abstract":"A study was conducted to evaluate graft success, resistance to vine decline disease and effect of grafting on quality and yield of muskmelon. Two field experiments, conducted in Oman, showed that muskmelon cv. Sawadi grafted on six cucurbit rootstocks gave high grafting success: 97.6-99.1% (avg. 98.6%) and 92.4-96.9% (avg. 95.3%) under field conditions in fall 2012 and spring 2013 respectively. No significant differences were observed between seasons among the six treatments and a non-grafted control in consumer preference for odor and firmness, fruit shape, flesh vitamin C, micronutrient content and TSS (sucrose %) or pH in spring 2013 (P > 0.05). The concentration of phosphorus and sodium significantly decreased in both seasons in all treatments in comparison to the control (P > 0.05). In both seasons potassium content significantly increased when Rsscih7458 and Mubyeongjangsoo rootstocks were used (P < 0.05). Strong Tosa rootstocks showed zero graft failure, high resistance to vine decline disease, high yield production and higher TSS (sucrose %) as compared to other rootstocks. Strong Tosa and Tetsukabuto rootstocks showed significantly higher consumer acceptance for rind color, flesh color and overall consumer acceptance in spring 2013 and was also less affected by seasonal changes. Results suggested that by grafting muskmelon cv. Sawadi some quality attributes may be improved in addition to the increased level of resistance to disease. However, additional trials are required to make final recommendations for the farming community.","PeriodicalId":355315,"journal":{"name":"Journal of Agricultural and Marine Sciences [JAMS]","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124091738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}