Tsitologiya最新文献_第10页

Роль родительского происхождения хромосом в нестабильности соматического генома клеток мозга дрозофилы и формировании памятного следа в норме и при стрессе 染色体父母在果蝇脑细胞的躯体基因组不稳定和压力形成记忆痕迹方面的作用是正常的。

Tsitologiya Pub Date : 2019-01-01 DOI: 10.1134/s0041377119120071

С. А. Васильева, Е. В. Токмачева, А. В. Медведева, А. А. Ермилова, Е. А. Никитина, Б. Ф. Щеголев, С. В. Сурма, Е. В. Савватеева-попова

引用次数: 0

Онкосупрессорные свойства раково-тестикулярных антигенов SEMG1 и SEMG2 в клеточной модели карциномы поджелудочной железы человека MIA-PACA2 肿瘤-睾丸抗原SEMG1和SEMG2在细胞模型中作为人类胰腺癌-PACA2。

Tsitologiya Pub Date : 2019-01-01 DOI: 10.1134/s0041377119110099

О. Ю. Шувалов, А. И. Кизенко, А. В. Петухов, С. Е. Парфеньев, О. А. Федорова, А. А. Дакс, Н. А. Барлев

引用次数: 0

Чувствительность культивируемых нейральных стволовых клеток мыши к действию ионизирующего излучения 老鼠培养的神经干细胞对电离辐射作用的敏感度

Tsitologiya Pub Date : 2019-01-01 DOI: 10.1134/s0041377119100067

Галина Ароновна Посыпанова, Мария Григорьевна Ратушняк, Юлия Павловна Семочкина, А. А. Абишева, Елизавета Юрьевна Москалева

引用次数: 0

PATHOMORPHOLOGICAL CHANGES OF THE SKELETAL MUSCLES UNDER FORMATION OF THE REPERFUSION SYNDROME. 再灌注综合征形成时骨骼肌的病理形态学改变。

Tsitologiya Pub Date : 2017-01-01

L V Anisimova, A V Kubyshkin, L L Aliev, Ye Yu Bessalova, V Z Kharchenko

{"title":"PATHOMORPHOLOGICAL CHANGES OF THE SKELETAL MUSCLES UNDER FORMATION OF THE REPERFUSION SYNDROME.","authors":"L V Anisimova, A V Kubyshkin, L L Aliev, Ye Yu Bessalova, V Z Kharchenko","doi":"","DOIUrl":"","url":null,"abstract":"Analysis of pathomorphological changes which taking place in muscle tissue after reperfusion of previously\u0000ischemic rats limbs allowed to identify three phases of the experimental reperfusion syndrome (RS): the\u0000first or ischemic, the second or initial reperfusional, the third or late reperfusional. Morphological changes of\u0000the skeletal muscles in the first stage are characterized by presence of dystrophic-necrotic processes and reflect\u0000the compensatory-adaptive reaction of the organism to hypoxia. In the third stage one can see the progress of\u0000morphological damages, which develop during the ischemic period against a background of exhaustion of\u0000proteinase inhibitors. This indicates the intensity of endogenous intoxication of the organism with the products\u0000of disturbed metabolism and determines the irreversibility of destructive processes and probability of multiple\u0000organ failure. Proceeding from the character of the pathomorphological changes and the state of proteinase-inhibitor system one can suppose, that the optimal time for medical measures is the first stage and the first hours of the second stage (to increase the ischemic tolerance of the skeletal muscles). Taking into account the direct relation between the intensity of pathomorphological injuries and the imbalance of proteinase-inhibitor system, the usage of proteinase-inhibiting medicines for correction of RS-development and reduction of the destructive changes during first and second stages is substantiated. When reperfusion syndrome lasts for a long time, medical measures become ineffective due to the high degree of pathological changes.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36463139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE STRUCTURAL CHANGES OF MACROPHAGES INFECTED WITH TICK-BORNE ENCEPHALITIS VIRUS. 蜱传脑炎病毒感染巨噬细胞的结构变化。

Tsitologiya Pub Date : 2017-01-01

N G Plekhova, E V Pustovalov, L M Somova, G N Leonova, E I Drobot, I N Lyapun

{"title":"THE STRUCTURAL CHANGES OF MACROPHAGES INFECTED WITH TICK-BORNE ENCEPHALITIS VIRUS.","authors":"N G Plekhova, E V Pustovalov, L M Somova, G N Leonova, E I Drobot, I N Lyapun","doi":"","DOIUrl":"","url":null,"abstract":"Macrophages belong to the innate immune cells and play a key role in the pathogenesis of viral infections.\u0000The results of ultrastructural study of macrophages infected with tick-borne encephalitis virus (TBEV), the Flavivirus\u0000family, pathogens of human infections, affecting the nervous system, were presented. With the assistance\u0000of virological methods was found that the TBEV are absorbed by macrophages and replication in them. An\u0000ultrastructural study has shown that the virus enters into the cytoplasm by local destruction of plasmalemma\u0000and newly synthesized virus particles exited from the cell by same. Simultaneously there is a seal of perinuclear\u0000cytoplasm space, where found in a large number of ribosomes, microfilaments, ribonucleoprotein fibers and viral\u0000special structure: nucleocapsids, tubular formations and viral layers (fabrics). On the surface of last structures\u0000the newly synthesized virus particles were visualized. Thus, the evidence shows that macrophages play a role\u0000in the spread of TBEV, being for their the target cell. As active antigen presenting cells the macrophages can\u0000modulate the protective response of the body and influence on the pathogenesis of tick-borne encephalitis.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36463763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRODUCTION OF SINGLE CHAIN FRAGMENT VARIABLE (scFv) ANTIBODY AGAINST PARANOSEMA LOCUSTAE ALPHA/BETA-HYDROLASE AND IMMUNOLOCALIZATION OF MICROSPORIDIAN ENZYME IN THE INFECTED HOST CELL. 单链片段可变抗体(scFv)的产生和微孢子虫酶在被感染宿主细胞中的免疫定位。

Tsitologiya Pub Date : 2017-01-01

V V Dolgikh, A A Tsarev, I V Senderskiy, S A Timofeev

{"title":"PRODUCTION OF SINGLE CHAIN FRAGMENT VARIABLE (scFv) ANTIBODY AGAINST PARANOSEMA LOCUSTAE ALPHA/BETA-HYDROLASE AND IMMUNOLOCALIZATION OF MICROSPORIDIAN ENZYME IN THE INFECTED HOST CELL.","authors":"V V Dolgikh, A A Tsarev, I V Senderskiy, S A Timofeev","doi":"","DOIUrl":"","url":null,"abstract":"Microsporidia is a widespread group of fungi-related intracellular parasites. Direct contact of the most microsporidia species with host cytoplasm suggests that these parasites may control physiological processes of infected cells by secretion of various proteins. In previous experiments, secretion of significant amounts of microsporidia Paranosema locustae alpha/beta-hydrolase into infected cells of Locusta migratoria fat bodies was demonstrated using polyclonal antibodies against the enzyme. However, heterologous expression of microsporidian hydrolase in yeast Pichia pastoris cells was not accompanied by its secretion. In this study, we have constructed library of recombinant single chain antibodies (scFv-fragments) against proteins of fat bodies of infected locusts and isolated mini-antibody specifically recognizing the studied enzyme using phage display technology. Immunoblotting and immunofluorescent microscopy with selected scFv-fragment confirmed secretion of two different in size forms of P. locustae alpha/beta-hydrolase into infected host cell. Prospects of scFv-fragment use to explore the role of microsporidian hydrolase in host-parasite relations and mechanism of its secretion are discussed in the paper.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36467366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE. ITLN1、PPARg和TNFa基因在内脏脂肪组织中的表达。

Tsitologiya Pub Date : 2017-01-01

T S Usenko, V V Miroshnikova, E A Bazhenova, M A Nikolaev, D L Brovin, A E Kopytova, A A Panteleeva, J He, I A Semenova, N D Razgildina, A E Neimark, O A Berkovich, O D Belyaeva, E I Baranova, S N Pchelina

{"title":"ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE.","authors":"T S Usenko, V V Miroshnikova, E A Bazhenova, M A Nikolaev, D L Brovin, A E Kopytova, A A Panteleeva, J He, I A Semenova, N D Razgildina, A E Neimark, O A Berkovich, O D Belyaeva, E I Baranova, S N Pchelina","doi":"","DOIUrl":"","url":null,"abstract":"The adipose tissue is considered today as an endocrine organ in which tissue-specific regulation of gene expression plays a key role in the processes of development of obesity and comorbidities, such as diabetes and cardiovascular disease. The present study is focused on ITLN1, PPARã and TNFá gene expression in intra-abdominal adipose tissue and its effect on the serum levels of omentin 1 and TNFa in individuals with different body mass. It has been shown that serum TNFa level is significantly higher in the subgroup of patients with overweight and obesity (BMI ≥ 25 kg/m2) as compared to individuals with normal body weight (BMI < 25 kg/m2)( p < 0.03). We have demonstrated that the expression level of the PPARã gene is positively correlated with the ITLN1 gene expression level in the intra-abdominal adipose tissue (r = 0.516, p = 0.020). Serum level of omentin 1 positively correlates with PPARã mRNA and protein levels in intra-abdominal adipose tissue (r = 0.550, p < 0.05 and r = 0.581, p < 0.03, respectively). For the subgroup of patients with overweight and obesity, we have shown negative correlation of the level of TNFá mRNA with PPARã and ITLN1 mRNA levels was shown (r = –0.549, p < 0.05 and r = –0.475, p < 0.05, respectively). This study is the first to show a correlation relationship between PPARã gene expression level in the intra-abdominal adipose tissue and the expression and secretion levels of omentin 1.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36467845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROLE OF Wip1-p53 AXIS IN RESPONSE OF MURINE CELLS TO TREATMENT WITH SODIUM BUTYRATE AND MEK/ERK SIGNALLING PATHWAY INHIBITOR. Wip1-p53轴在小鼠细胞对丁酸钠和MEK/ERK信号通路抑制剂的反应中的作用。

Tsitologiya Pub Date : 2017-01-01

E ayu Kochetkova, O N Demidov

{"title":"ROLE OF Wip1-p53 AXIS IN RESPONSE OF MURINE CELLS TO TREATMENT WITH SODIUM BUTYRATE AND MEK/ERK SIGNALLING PATHWAY INHIBITOR.","authors":"E ayu Kochetkova, O N Demidov","doi":"","DOIUrl":"","url":null,"abstract":"The use of histone deacetylase inhibitors and inhibitors of MEK/ERK-pathway is proposed as a novel potential approach in cancer treatment. Here we studied the effects of histone deacetylase inhibitor, sodium butyrate, and MEK/ERK-pathway inhibitor, PD0325901, on cells with modifications in genes involved in anti-cancer therapy response, Wip1 phosphatase and p53. We have investigated the effect of these agents on cell cycle of wild-type cells, Wip1 knockout cells and cells with double deletion of Wip1 and p53. Our results showed that more severe changes in S and G2/M phases were observed in response to sodium butyrate in Wip1-defecient cells than in wild-type cells. Meanwhile, PD0325901 treatment led to G1 arrest. At the same time, a «sodium butyrate type» response dominated the response to combined treatment with both drugs in Wip1-deficient cells, while the response of Wip1–/–/p53–/– cells to combined treatment was similar to the single use of PD0325901. Wip1–/– and Wip1–/–/p53–/– cells were more sensitive to the use of PD0325901 than wild-type cells. Obtained results suggest that Wip1 deficiency sensitizes cells to sodium butyrate and to MEK/ERK inhibitors independently from Wip1 main target protein — p53. Data acquired give insights into role of Wip1 in cellular responses to treatment with HDAC and MEK/ERK inhibitors.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36468475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

REORGANIZATION OF INTERPHASE MICROTUBULES IN ROOT CELLS OF MEDICAGO SATIVA L. DURING ACCLIMATION TO OSMOTIC AND SALT STRESS CONDITION. 渗透和盐胁迫下紫花苜蓿根细胞间期微管的重组。

Tsitologiya Pub Date : 2017-01-01

E M Lazareva, E N Baranova, E A Smirnova

{"title":"REORGANIZATION OF INTERPHASE MICROTUBULES IN ROOT CELLS OF MEDICAGO SATIVA L. DURING ACCLIMATION TO OSMOTIC AND SALT STRESS CONDITION.","authors":"E M Lazareva, E N Baranova, E A Smirnova","doi":"","DOIUrl":"","url":null,"abstract":"We have investigated the organization of microtubule system in interphase cells of Medicago sativa L. roots during acclimation to salt and osmotic stress at different concentrations of NaCl, Na2SO4 and mannitol. We have identified several morphological changes in tubulin cytoskeleton that appear during the acclimation to salt and osmotic stress in the cells of different root tissues: 1) decreased density of cortical microtubule network, 2) random orientation of cortical microtubule bundles, 3) non-uniform density of the bundles, 4) thickening of the bundles, 5) fragmentation of the bundles, 6) formation of centers of converging microtubule. Reduced density of the microtubule network and thickening of the bundles was detected during osmotic and salt stress, yet random orientation of cortical microtubules was observed under osmotic stress and not found during salt stress. Fragmentation of microtubule bundles was apparent during salt stress and less evident at high concentration of mannitol. Formation of centers of converging microtubule was common under prolonged action of sodium sulfate, less common under sodium chloride and not found after mannitol treatment. Our data show that cortical microtubules in alfalfa root cells rearrange not only in response to different ions, but also to osmotic pressure. Thus, the signaling pathways and molecular mechanisms inducing reorganization of the microtubule system may be triggered not only by sodium cations but also by sulfate and chloride anion at the concentrations that do not cause irreversible cell damage. Our study show that the osmotic and salt stress differently affect the cortical microtubules, and their reorganization in response to stress depends on the salt cations as well as anions might also show additional effect under salt stress.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36467846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE ROLE OF LMNA MUTATIONS IN MYOGENIC DIFFERENTIATION OF PRIMARY SATELLITE CELLS AND C2C12 CELLS. lmna突变在原代卫星细胞和c2c12细胞成肌分化中的作用。

Tsitologiya Pub Date : 2017-01-01

K I Perepelina, N A Smolina, A S Zabirnik, R I Dmitrieva, A B Malashicheva, A A Kostareva

{"title":"THE ROLE OF LMNA MUTATIONS IN MYOGENIC DIFFERENTIATION OF PRIMARY SATELLITE CELLS AND C2C12 CELLS.","authors":"K I Perepelina, N A Smolina, A S Zabirnik, R I Dmitrieva, A B Malashicheva, A A Kostareva","doi":"","DOIUrl":"","url":null,"abstract":"Nuclear lamins form nuclear lamina localized under the inner nuclear membrane. It was previously considered\u0000that the nuclear lamina predominantly plays a structural role, however, its involvement have been recently\u0000described in the regulatory processes such as chromatin organization and gene transcription. It is known that\u0000mutations in the LMNA gene lead to the development of a large number of diseases, laminopathies, which mainly\u0000affect mesenchymal tissue. Nowadays, the mechanisms by which the lamina can regulate cell differentiation\u0000remain incompletely understood. In the present work, we have studied the effect of LMNA gene mutations on\u0000the process of muscle differentiation of primary satellite cells and Ñ2Ñ12 cell line. The genome of satellite cells\u0000and Ñ2Ñ12 cell line was modified by the introduction of lentiviral constructs encoding LMNA G232E associated\u0000with the development of muscular dystrophy Emery—Dreyfus and LMNA R571S associated with the development\u0000of dilated cardiomyopathy. The morphology of the cells was estimated using immunofluorescence, the\u0000expression level of myogenic genes were analyzed by qPCR. We have shown that the analyzed mutations reduce\u0000the ability of cells to differentiate, to fuse and to form myotubes. We have suggested that it is due to enhanced\u0000expression of markers at the early stages and to reduced expression markers at the late stages of myogenesis.\u0000Therefore, mutations in nuclear lamins can influence the process of muscle differentiation.","PeriodicalId":35517,"journal":{"name":"Tsitologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36477548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0