Open Journal of Regenerative Medicine最新文献

{"title":"Autologous Adipose-Derived Mesenchymal Stem Cells Embedded in Platelet-Rich Fibrin in Diabetic Foot Ulcers","authors":"C. Khalil, D. Chaker, Rawad Salameh, Yvonne-Elise Germanos, Elie El Kayem, Fady Nader, J. Habbouche, R. Chemaly, A. Azar, A. Ibrahim","doi":"10.4236/ojrm.2021.102002","DOIUrl":"https://doi.org/10.4236/ojrm.2021.102002","url":null,"abstract":"Context and Aim: Mesenchymal stem cells (MSCs) and platelet-rich fibrin (PRF) have emerged as ideal candidates for advanced therapies of various therapeutically-challenging diseases; however, their regenerative potential in diabetic foot ulcers (DFU) has not been well determined. In this study, we reviewed our clinical experience in mitigating chronic ulcer complications of diabetic foot through a conventional treatment of autologous adipose-derived MSCs embedded in PRF with pure PRF injections. Materials and Methods: The present study was carried out in 10 patients with an open DFU wound selected over a period of 1 year starting from April 2019. Patients were either injected with PRF alone (Group A) or injected with MSCs derived from adipose tissue (ADMSC) embedded in (PRF (Group B). Results: Patients in Group B had a better healing index when compared to Group A. Conclusion: Use of ADMSC embedded in PRF showed promising results to treat DFU.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131612496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cosmetic Applications of Regenerative Medicine—A New Approach to the Reduction of Neck Lines","authors":"E. R. Vickers, Haitao Wen","doi":"10.4236/ojrm.2020.92005","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92005","url":null,"abstract":"Combining different areas of regenerative medicine is considered the new standard for comprehensive tissue and organ regeneration. This approach can be further applied to cosmetic issues such as deep neck lines. This case report utilized techniques of autologous stem cell therapy and epigenetic tissue environment modulation with externally applied polyphenols and tissue penetration agents. The results showed a remarkable cosmetic improvement with a significant reduction of neck lines in a patient where employment and psychological issues were developing from the original problem. The regenerative medicine protocol was safe, efficient in disposable materials, portable benchtop equipment and surgical operating time. The patient had an excellent outcome for this difficult to treat cosmetic problem.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123227809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating 2019-nCoV Amidst the Pandemic Scare","authors":"K. Haider, Q. Hyder","doi":"10.4236/ojrm.2020.92003","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92003","url":null,"abstract":"2019-nCoV is the third consecutive coronavirus spread during the last 2 decades, but this time, unlike the previous two occasions, has achieved a pandemic proportion threatening widespread loss of human lives and a massive setback to the global economy. The situation warrants drastic measures in terms of preventing the spread of the virus and treating the virus-infected patients. The development of a new vaccine is a time-intensive option. Although efforts are underway to find possible pharmacological options, e.g., chloroquine, hydroxychloroquine, existing antiviral agents, etc., in the meanwhile we may work on the combinatorial interventional approach of combining drug therapy with passive immune therapy. It would be prudent to use a convalescent serum therapy approach with the serum from COVID-19 patients who have recovered from the infection.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127832926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal Stem Cells (MSCs) as a Novel Therapeutic Option for nCOVID-19—A Review","authors":"Madhan Jeyaraman, Rajeswari Somasundaram, T. Anudeep, Ajay Ss, Kumar V. Vinodh, R. Jain, M. Khanna","doi":"10.4236/ojrm.2020.92004","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92004","url":null,"abstract":"The novel Coronavirus Disease (nCOVID-19) is a highly contagious viral infection which emerged as “Pneumonia of Unknown Etiology” at Hubei province of Wuhan city in China. The health authorities provided a considerable empirical evidence after this outbreak and it was notified that the causative virus, named Novel Coronavirus (subsequently SARS-CoV-2) is the culprit for progressively exerting grim effects not only on individual patients but also on the International public health, with high mutational tendencies. WHO declared nCOVID-19 as a Pandemic on 11th March 2020. The spike glycoprotein of SARS-CoV-2 plays a pivotal role in the entry of virus into the cell and it further interacts with ACE-II receptors which are widely distributed on the human cell surface especially on alveolar type II cells (AT-2) and endothelium. The mortality in nCOVID-19 patients is usually preceded by acute respiratory distress syndrome (ARDS) because of the cytokine storm. Advanced molecular biology and regenerative sciences renders a breakthrough in the treatment of severely ill nCOVID-19 patients with Mesenchymal Stem Cells (MSCs). Autologous or allogenic MSCs attenuate cytokine storm, improvise lung compliance, regulate inflammatory response, maintain functional alveoli microenvironment, promote endogenous regeneration and repair with no or minimal side effects. MSCs are naturally resistant to this novel Coronavirus. Even though it is corroborated with evidences from current clinical trials and pilot study, we emphasize the need for conducting more clinical trials with ethical consideration to prove the efficacy and safety of MSCs in combating nCOVID-19 infection and its complications.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130801577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Medical Devices Using Spongy Sheet Composed of Hyaluronic Acid and Collagen","authors":"Y. Kuroyanagi, Haruka Ohno, Ryusuke Suzuki, Misato Kuroyanagi, I. Takeuchi","doi":"10.4236/ojrm.2020.92008","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92008","url":null,"abstract":"This research aims to \u0000obtain useful information for development of medical devices such as wound \u0000dressing and tissue anti-adhesive product, using a spongy sheet composed of \u0000hyaluronic acid (HA) and collagen (Col). The spongy sheets were manufactured by \u0000freeze vacuum drying of HA and Col aqueous solution, followed by UV irradiation \u0000to introduce intermolecular crosslinks between Col molecules. These spongy \u0000sheets are referred to as Sponge-A (ratio of HA/Col = 5/1) and Sponge-B (ratio \u0000of HA/Col = 5/5). Both surfaces of Sponge-A and Sponge-B treated with UV \u0000irradiation for 15 minutes are referred to as Sponge-A-15 and Sponge-B-15, \u0000respectively. The weight change of spongy sheet was determined by immersing a \u0000peace of spongy sheet in water at 37°C. The weight of sponge-A-15 collected 1/2, 1, 3, 7 days after immersion \u0000in water was 63.5%, 62.1%, 56.6%, 54.4% of the original weight, respectively. \u0000The weight of Sponge-B-15 was 78.3%, 76.7%, 79.1%, 71.9% of the original \u0000weight, respectively. The weight change of spongy sheet was determined by \u0000immersing a peace of spongy sheet in water containing collagenase at 37°C. The weight of Sponge-A-15 collected 6, 8, 10, 12 \u0000hours after immersion in water containing collagenase (0.0005 w/v%) was 65.7%, 59.8%, \u000057.9%, 55.2% of the original weight, respectively. The weight of Sponge-B-15 \u0000was 63.5%, 52.1%, 42.0%, 43.2% of the original weight, respectively. This \u0000spongy sheet is considered to have the unique structure, where HA molecules are \u0000entrapped in an intermolecular cross-linked network structure of Col molecules. \u0000When immersed in water containing collagenase, the weight loss of spongy sheet \u0000is accelerated by easy extraction of HA molecules from the enzymatic degraded \u0000Col network structure. The performance of wound dressing and tissue anti-adhesive \u0000product is considered to depend on appropriate ratio of HA and Col, and also on \u0000appropriate rate of intermolecular crosslinks between Col molecules. These \u0000findings obtained in this study provide useful information for product \u0000development such as wound dressing and tissue anti-adhesive product.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132872086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Drug Therapy to Combat Coronavirus","authors":"Shouguo Wang, Wei Liu, Y. Song, Tao Xia, Xianqin Lu, Li Song, Qingwei Li, Jian Sun, Xu Yin, Xianqi Feng, Yunjun Yang, Taotao Sun","doi":"10.4236/ojrm.2020.92007","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92007","url":null,"abstract":"Novel coronavirus is threatening the health of people all over the world and all possible treatment strategies are in urgent. The sensitive of virus to heat proposed temperature treatment as an effective method against coronavirus. Here, we proposed several non-drug therapies including far-infrared, thermal air, thermal oxygen, ozone, hydrogen therapy and plasma physical therapy for immediate clinical trial and implementation. Compared with the drug therapy, these methods are safe without side effects, the highest benefit-to-risk ratio, user-friendly, and low implementation costs. It is urgent and significant to start these non-drug therapeutic design and implementation as early as possible, to determine the safety and effectiveness of these therapeutic methods.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115450393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 Pandemic: Its Origin, Implications and Treatments","authors":"P. Law","doi":"10.4236/ojrm.2020.92006","DOIUrl":"https://doi.org/10.4236/ojrm.2020.92006","url":null,"abstract":"This is a succinct and current review of pertinent literature to guide developing serum therapy as an emergent treatment to save human lives at times of natural or genetically engineered viral/bacterial pandemics. The origin of 2019-nCoV and implications of COVID-19 are discussed using direct quotes of published scientific literature to avoid misinterpretation on this very important event that has caused great loss of human lives and international social economy. It is the goal of this review to warn against and to correct international misunderstanding created by deliberate falsification of scientific documentations and events. This misunderstanding may lead to further destruction of life, economy, and political relations. People should not be blind-sighted when making life decisions.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124230479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"System Engineering of Emergent Serum Therapy to Combat COVID-19 and Other Pathogenic Pandemics","authors":"P. Law","doi":"10.4236/ojrm.2020.91002","DOIUrl":"https://doi.org/10.4236/ojrm.2020.91002","url":null,"abstract":"Life-saving procedures of an emergency protocol to combat fatal ambushes of viral or bacterial pathogens are listed below for immediate dissemination and implementation. This unique protocol of serum therapy commands the highest benefits-versus-risks ratio, and is safe, efficacious, user-friendly and inexpensive to implement. A formal evaluation is presented to support upgrading convalescent plasma therapy to serum therapy in the context of presenting fewer donor antigens, thus evoking less lethal sensitization to obtain greater safety and efficacy in treatment.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125930226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concerns about Diabetic Foot Wounds","authors":"D. Solomon","doi":"10.4236/OJRM.2019.81001","DOIUrl":"https://doi.org/10.4236/OJRM.2019.81001","url":null,"abstract":"This paper in a medical sense can be described as a case report. The diabetic foot wounds of a relative, over 70 years of age, with Type I diabetes were so grievous and the medical treatment was so protracted that success could not be assured. There was a danger of amputation. The one outstanding feature was that the private application of an antifungal spray, between the toes of both feet without prescribed oral mycologic agents, seemed to make an inexplicable difference to the final outcome. In addition, a viewpoint drawn was that the efficacy of antibiotic therapies may be compromised by the presence of invisible fungal etiology.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132752821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erythrocyte Membrane Protein Band 3 Predicts Interferon Ribavirin-Induced Anemia","authors":"E. Altıntaş, S. Yalın, O. Sezgin, E. Uçbilek, A. Tombak, A. Kanık","doi":"10.4236/ojrm.2019.82002","DOIUrl":"https://doi.org/10.4236/ojrm.2019.82002","url":null,"abstract":"Aim & Background: It was proposed that the differences in erythrocyte membrane protein contents—especially band 4—take a role in the serious anemia related to interferon plus ribavirin (I/R). The aim of this study is to evaluate whether the erythrocyte membrane protein contents predict anemia related to I/R or not. Methods: 180 mcg interferon α 2a once a week and weight adjusted ribavirin daily were given for 48 weeks to fifty patients with chronic hepatitis C. It was diagnosed as anemia when haemoglobin concentration was <10 mg/dL. In the beginning, the erythrocyte membrane protein contents of all patients were separated by “Sodium Dodecyl Sulphate Polyacrylamide Gel Disc Electrophoresis (SDS-PAGE)” and haemoglobin concentrations were measured. Results: Anemia developed in 17 patients (34%). The levels of erythrocyte membrane proteins were as; spectrin: 20.468 ± 2.5902, ankyrin: 4.576 ± 1.2706, B3: 19.240 ± 2.8358, B4.1: 5.628 ± 1.8832, and B4.2: 5.848 ± 1.8030. When the relation between the development of anemia and erythrocyte membrane proteins was investigated, a relation was only found at B3 which was not statistically significant (p = 0.058). When ROC analysis was performed, 95% CI p = 0.035 for B3 (0.517 - 0.792) was found. In patients whose B3 level was below 17.7%, the sensitivity of anemia development risk was calculated as 64.7% and the specificity thereof was calculated as 66.7%. Erythrocyte membrane protein contents by gender were only different at B3 (p = 0.042). Anemia developed in 17 patients (34%). 14 of these patients were of female and 3 were of male gender; the gender played a significant role in terms of anemia (p = 0.003). Conclusions: Erythrocyte membrane protein B3 is not only useful in predicting the patient under the risk of developing anemia, but also it may be useful in preventing it and it may explain why women inclined to anemia.","PeriodicalId":351416,"journal":{"name":"Open Journal of Regenerative Medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130112101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}