A. Tokenova, G. Sitpayeva, N. Gemejiyeva, S. Suleimenova, N. Friesen, D. Batayeva
{"title":"Wild Allium longicuspis Regel is a Feral Form of Allium sativum L. in Kazakhstan: A Comparative Molecular Genetic Analysis","authors":"A. Tokenova, G. Sitpayeva, N. Gemejiyeva, S. Suleimenova, N. Friesen, D. Batayeva","doi":"10.3844/ojbsci.2023.33.43","DOIUrl":"https://doi.org/10.3844/ojbsci.2023.33.43","url":null,"abstract":": The genus Allium L comprises over 1000 species with important nutritional, medicinal and horticultural applications. The natural flora of Kazakhstan is rich in Allium species, including the wild long-pointed onion, A. longicuspis and cultivated A. sativum garlic varieties. It has been hypothesized that A. longicupis is ancestral to A. sativum . We investigated genetic polymorphism in samples of A. longicupis and the A. sativum cultivars “Niki” and “Merey” using start codon targeted polymorphism analysis. Nuclear ribosomal DNA fragments were purified, amplified and tested with 25 primers, finally selecting 12 primers that identified polymorphisms. DNA samples were sequenced, electrophoresed and cluster analysis performed by the construction of dendrograms and principal component analysis. The karyotypes of the different samples were also compared. The results showed that the samples of A. longicupis from natural populations did not differ significantly from those of the cultivated garlic varieties. The results indicate that A. longicupis can be considered a feral, rather than an ancestral, form of A. sativum . These findings have implications both for the breeding and cultivation of garlic varieties and for the taxonomy of the Allium genus.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44862896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Babich, B. Aryngaziev, L. Ovchinnikova, A. Ovchinnikov
{"title":"Features of Growth and Development in Heifers of Holstein and Black-and-White Breeds","authors":"E. Babich, B. Aryngaziev, L. Ovchinnikova, A. Ovchinnikov","doi":"10.3844/ojbsci.2022.529.538","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.529.538","url":null,"abstract":": The study of the characteristics of the growth and development of farm animals is of interest in breeding, as it will help to improve animal breeds, as well as serve for the organization of fast and economically rational raising of productive livestock. The research aims to study the characteristics of growth and development and indicators of reproduction of the Holstein and Black-and-White breeds. For research, three groups of 10-day-old heifers were formed. Body weight was considered by individual weighing at birth, at 3, 6, 9, 12, 15 and 18 months. The reproductive indices of heifers were studied according to the data of the log of inseminations and calving of cows: The age at the first insemination, the age at fertilization and the number of inseminations per 1 fruitful insemination were considered. The conducted studies led to a conclusion that the Holstein heifers had an advantage in live weight over the Black-and-White heifers at the age of 6 months by 19.5 kg and 15.5 kg (P≤0.001), at 12 months of age by 18.4 kg (P≤0.01) and 19.2 kg, at 18 months by 28.0 kg and 25.3 kg (P≤0.01), respectively and exceeded them in growth intensity during the entire study period. The Holstein animals matured faster and were inseminated 43.2-45.8 days earlier than the Black-and-White animals that were inseminated at 511 days. Profits from raising the Holstein heifers were higher by $48.79-54.79.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46696111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Vesselova, K. Makhmudova, G. Kudabayeva, B. Osmonali, V. Mikhalev
{"title":"Current Growth Conditions of Populus Diversifolia Schrenk and Populus Pruinosa Schrenk in the Syr-Darya Valley","authors":"P. Vesselova, K. Makhmudova, G. Kudabayeva, B. Osmonali, V. Mikhalev","doi":"10.3844/ojbsci.2022.425.438","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.425.438","url":null,"abstract":": In the context of urbanization of territories and the intensification of agriculture, the biological diversity of animals and plants in their habitats is degrading. The purpose of the study is to determine the edaphic factors affecting the growth of Populus diversifolia Schrenk and Populus pruinosa Schrenk, as well as anthropogenic factors affecting their conservation in the Syr-Darya valley (Kazakhstan). For identifying the range of edaphic growing conditions, the analysis of soil samples gathered in different phytocenoses with the dominance or participation of Turanga was carried out. The following results were presented: Physical and chemical analyses of soil samples for the content of humus, mobile forms of easily hydrolyzable nitrogen, phosphorus, and potassium; pH aqueous solution; type, and degree of salinization. Among the 6 sections studied, 4 belong to alluvial-meadow tugai salt marsh soils, 1-to meadow salt marshes, and 1-to alluvial-meadow strongly salt marsh soils. Populus diversifolia has been found to prefer mild (sandy) and fewer saline conditions than Populus pruinosa . Among the negative anthropogenic impacts on the Turanga groves were: Regulation of the runoff of the Syr-Darya, felling of trees, fires, and grazing. The identified cenoflora was represented by 103 species of higher vascular plants. Among them, Chenopodiaceae (18), Asteraceae (18), and Brassicaceae (12) were leading. The indicative role of Turanga was based on its connection to the places of groundwater occurrence.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45743627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Adilkhankyzy, K. Alpysbayeva, B. Nurmanov, B. Z. Naimanova, N. Bashkarayev, A. Kenzhegaliev, A. Uspanov
{"title":"Integrated Protection of Tomato Crops against Tuta absoluta in Open Ground Conditions in the South-East Part of Kazakhstan","authors":"A. Adilkhankyzy, K. Alpysbayeva, B. Nurmanov, B. Z. Naimanova, N. Bashkarayev, A. Kenzhegaliev, A. Uspanov","doi":"10.3844/ojbsci.2022.539.548","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.539.548","url":null,"abstract":": There is a need to develop strategies for the protection of tomato crops that would provide high-quality produce safe for the consumer without environmental pollution. The objective of the study is to deploy an integrated system for the protection of open-field tomato crops from Tuta absoluta using biological agents ( Bracon hebetor Say., Trichogramma, biopreparations, pheromone traps) and devices in the conditions of southeast Kazakhstan to obtain environmentally safe produce. Experiments on the protection of tomato crops from pests are conducted by applying integrated protection measures with the use of entomophages, biopesticides, pheromone traps, and low-hazard pesticides on the tomato field of the Musa private farm. Per the results of the experiments on protecting open-field tomato plants from Tuta absoluta, the effectiveness of the employed instruments reaches up to 90%. The obtained results can be used to protect tomato plants accounting for pest density and the phase of crop development. Thus, based on the results obtained, it can be concluded that the primary elements of the integrated system for the protection of tomato crops against Tuta absoluta should include the application of biological preparations, entomophages and, if necessary, low-hazard insecticides, as well as the use of pheromone traps to detect the appearance of the moths and capture them en masse.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43015998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Glazunov, Yana Kabitskaya, L. Glazunova, I. Donnik, E. Boyko, Y. Vinogradova
{"title":"Participation of Dermacentor Reticulatus Imago in the Reservation of Bovine Leukemia Virus","authors":"Y. Glazunov, Yana Kabitskaya, L. Glazunova, I. Donnik, E. Boyko, Y. Vinogradova","doi":"10.3844/ojbsci.2022.456.462","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.456.462","url":null,"abstract":"","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42868972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Retraction Notice: Review of Prodigiosin, Pigmentation in Serratia marcescens","authors":"Notice","doi":"10.3844/ojbsci.2022.549","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.549","url":null,"abstract":"","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43958353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Tyrzhanova, Margarita Y Ishmuratova, S. N. Atikeeva, A. Kydyrmoldina, Meruyert Malikovna Malik
{"title":"Biological Study Scabiosa ochroleuca Seeds Germination and Development of Methods for a Long-Term Storage","authors":"S. Tyrzhanova, Margarita Y Ishmuratova, S. N. Atikeeva, A. Kydyrmoldina, Meruyert Malikovna Malik","doi":"10.3844/ojbsci.2022.512.519","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.512.519","url":null,"abstract":": The organization of a storage system for seeds of medicinal plants is an important task of introduction. Our study aimed to identify biological features during the germination of Scabiosa ochroleuca seeds and optimize the conditions for their storage and germination activation. The paper presents the results of assessing the viability of seeds of S. ochroleuca depending on the origin, size, storage time, the use of pre-sowing treatment with epin, potassium humate, the use of barbotage and stratification; the conditions of cryopreservation in liquid nitrogen have been worked out. The results showed that germination capacity and energy depended on origin and size. The best indicators were noted for large and completed seeds collected in the Buyratau Mountains. During storage, a gradual decrease in germination was noticed; the loss of viability was noted in the 4 th year of storage under normal conditions. Stratification can be used for 3 months to activate germination. To organize a long-term storage system, we recommend storing S. ochroleuca seeds in liquid nitrogen in plastic containers, using glycerol cryoprotectant at a concentration of 40% .","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46562773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Beneficial Effects of Soybean and Cassava in Local Wisdom Feed Diets on Growth Performance with Nutritional and Economic Analysis of Snakehead Fish (Channa Striata)","authors":"D. Boonkusol, W. Tongbai","doi":"10.3844/ojbsci.2022.463.468","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.463.468","url":null,"abstract":": Substituting plant protein for fish meals is an alternate protein source in the diet of reared fish. By replacing fish meal with Soybean Meal (SM) or Cassava Meal (CM), this study determined the effects of diets influenced by local wisdom containing plant protein meal, SM, CM, or a commercial formula (FM TM ) on the survival and growth rates of snakehead fish ( Channa striata ) with nutritional and economic analysis. After acclimatization, snakehead fingerlings (10 fish/tank) were randomly allocated in the nine cement tanks. Three tanks each for Soybean Meal (SM) diet; Cassava Meal (CM) diet; and commercial diet (FM TM ). Fish weights were determined every four weeks for the 12 weeks. At the end of each experiment, the Survival Rate (SR), growth performance values, and cost reduction were calculated. After 12 weeks, there were no significant differences in SR among treatment groups. The growth performance of fish in SM dietary treatment and FM TM dietary treatments had no significant difference. Although, the specific growth rate of fish fed CM diet was slightly less than SM and FM TM diet .The soybean meal or cassava was substituted for a fish meal resulting in 11.6 and 7.9% economic value (cost/100g fish product), respectively compared to the commercial diet. However, the CM diet resulted in the lowest Feed Conversion Rate (FCR) in this experiment. There were no significant differences between FCR and Protein Efficiency Rate (PER). The results revealed the beneficial effect of diets influenced by local wisdom, especially the addition of soybean, in terms of weight gain promotion and feed cost reduction. These findings can be applied to develop low-cost diets for snakehead fish, supporting the flexibility and competitiveness of the snakehead fish farming industry and reducing the number of small fish used for fishmeal.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41854624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assylbek Abdrashitovich Zhanabayev, Birzhan Yeleubaevich Nurgaliev, A. Kereyev, A. Y. Paritova, Altay Y. Ussenbayev, S. Bayantassova, Dinara M. Seitkamzina, G. K. Zhumagaliyeva, Botakoz Yelemessova
{"title":"Parasite Fauna and Infection of Sheep with Parasitosis","authors":"Assylbek Abdrashitovich Zhanabayev, Birzhan Yeleubaevich Nurgaliev, A. Kereyev, A. Y. Paritova, Altay Y. Ussenbayev, S. Bayantassova, Dinara M. Seitkamzina, G. K. Zhumagaliyeva, Botakoz Yelemessova","doi":"10.3844/ojbsci.2022.404.414","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.404.414","url":null,"abstract":": The purpose of our research was to study the parasitic fauna and infection of sheep with parasites in the northern and southern regions of the Republic of Kazakhstan. For this, we conducted a study to determine the species diversity of sheep parasites. The study was set from 2010-2020 years on infected sheep in the northern and southern parts of the Republic of Kazakhstan. The experiments were conducted at the Department of Veterinary Medicine of S. Seifullin KazATU and the KazNIVI in Almaty. The species diversity of parasites of adult sheep was studied. The materials for parasitological laboratory studies were sheep feces taken from 50 sheep. Vital diagnostics of helminthiasis were carried out by scatological studies of 50 sheep using the well-known methods of Fulleborn, Weid, and Boyahchyan. To establish the timing of infection, selective coprolarvoscopic studies of fecal samples taken from pastures were carried out. First, the invasive larvae were isolated from fecal samples by the Berman-Orlov method. Differential diagnosis of trichostrongylides was carried out according to the method of Y.Y. Shumakovich. Among the sheep of the Akmola and Karaganda regions, strongylatoses parasitizing in the gastrointestinal tract are recorded: Nematodyrosis, ostertagiosis, hemonhoz, marshallagiasis, esophagostomosis, and, less often, bunostomosis. The development of eggs and larvae of the invasive stage in pasture conditions is noted at the end of the first decade of May. The first cases of infection of lambs are observed in late May-early June. Thus, based on the three-year results obtained, it can be concluded that the absence of antiparasitic measures leads to infection of almost all livestock within 5-7 months and in subsequent years, reinfection occurs, which ultimately causes a decrease in productivity and increase mortality, especially among young animals up to 1.5 years of age.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49632433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sana Maidoumi, Mariam Ouisselsat, Charif Radouan Ouaziz, Nadia Abbassi, M. Loukid, A. Ahami, A. Pineau, A. Sedki
{"title":"Visual-Spatial Attention Abilities Among School Children Exposed to Low Environmental Lead Level","authors":"Sana Maidoumi, Mariam Ouisselsat, Charif Radouan Ouaziz, Nadia Abbassi, M. Loukid, A. Ahami, A. Pineau, A. Sedki","doi":"10.3844/ojbsci.2022.439.447","DOIUrl":"https://doi.org/10.3844/ojbsci.2022.439.447","url":null,"abstract":": Lead (Pb) is an intrusive toxic matter which harms the neurocognitive and behavioral development of children even with doses considered normal. The present paper aims at evaluating the visual-spatial attention function among 142 schoolchildren living in a mining area (cases) in comparison to 177 living in the Chouiter area (control group) in Marrakech. We used the Bell Dam Test (BDT) for the assessment of their visual attentional abilities and unilateral spatial neglect. Interviews with parents were conducted to identify co-variables related to Pb exposure. Agricultural soil, drinking, and groundwater were collected from the surrounding area of the children's school. The determination of Pb content in the environment was performed by ICP-MS. The results demonstrated that cases' scores in the test of visual attentional abilities and in scanning procedures were significantly worse in comparison to the control group (p = 0.05 and p = 0.002, respectively). In cases, soil Pb level was higher (67.14±10.13 g/g) than in controls (41.73±13.6 g/g). Pb levels in groundwater and drinking water were low with a significant difference between the two areas in groundwater. The mean scores of visual attentions were significantly lower in children exposed to environmental Pb contamination compared to those who are not exposed (p = 0.008). These findings suggest that Pb is probably incriminated in the genesis of visual attention deficit.","PeriodicalId":35048,"journal":{"name":"OnLine Journal of Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48469918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}