Experimental Biomedical Research最新文献_第8页

The variation of 5 -carboxycytosine localization within the nucleus of normal and cancerous cells 5 -羧基胞嘧啶在正常细胞和癌细胞细胞核内定位的变化

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173851

Selcen Çelik Uzuner

引用次数: 0

Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazole-induced acute seizures in mice through GABA and glutamate mediated mechanism 选择性COX-2抑制剂美洛昔康通过GABA和谷氨酸介导的机制对戊四唑致小鼠急性癫痫发作表现出抗惊厥作用

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173850

B. Sahin, R. Akkaya, S. Karabulut

{"title":"Meloxicam, a selective COX-2 inhibitor, displays anticonvulsive effects in pentylenetetrazole-induced acute seizures in mice through GABA and glutamate mediated mechanism","authors":"B. Sahin, R. Akkaya, S. Karabulut","doi":"10.30714/j-ebr.2022173850","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173850","url":null,"abstract":"Aim: To investigate the possible anticonvulsive effect of the selective COX-2 inhibitor meloxicam in pentylenetetrazole (PTZ)-induced epileptic seizures in mice and to examine its possible role on inhibition and excitation balance in the brain. Method: 30 BALB-c albino mice (16-18 weeks old) weighing 30-33 gr were used. Animals were randomly divided into five groups (n = 6 for each group). Group 1: control, group 2: received saline (10 ml/kg, i.p.) 30 minutes before PTZ (60 mg/kg i.p.) administration, group 3: received saline (10 ml/kg, i.p.) 30 minutes after PTZ (60 mg/kg i.p.) injection, group 4: received 60 mg/kg meloxicam i.p., 30 minutes before PTZ (60 mg/kg i.p.) administration. Group 5: received meloxicam (60 mg/kg i.p.) 30 minutes after PTZ injection (60 mg/kg, i.p.). The animals were observed for 30 minutes and the seizure stages and first myoclonic jerk times (FMJ) were recorded. After 24 hours, brain tissues were removed and the cortex and hippocampus were separated for biochemical assessments. ELISA method was used to measure GABA and glutamate levels. Results: Administration of meloxicam before PTZ induced seizure, reduced seizure stages and prolonged FMJ duration (p<0.05). Pre-treatment with meloxicam increased GABA levels in the cortex and decreased glutamate levels in the hippocampus (p<0.05). Post-treatment of meloxicam after PTZ-induced seizure increased GABA levels in the hippocampus (p<0.05). Conclusion: The results of our experimental study suggest that meloxicam has anti-convulsive effects and these effects may be mediated by GABA and glutamate, which are the main indicators of inhibition and excitation balance in the brain.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"51 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132359473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of diffusion-weighted imaging on 3 tesla magnetic resonance in the clinical staging and pathological grading of clear cell renal carcinoma 3特斯拉磁共振弥散加权成像在透明细胞肾癌临床分期及病理分级中的作用

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173857

Hasan Gundogdu, R. Bedir, H. Eren, Berrin Erok

引用次数: 0

Riluzole and ranolazine application of prostate cancer: Cancer related testicular and liver tissue damage 利鲁唑和雷诺嗪在前列腺癌中的应用:癌症相关的睾丸和肝脏组织损伤

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173847

P. Aydın, I. Bugan

{"title":"Riluzole and ranolazine application of prostate cancer: Cancer related testicular and liver tissue damage","authors":"P. Aydın, I. Bugan","doi":"10.30714/j-ebr.2022173847","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173847","url":null,"abstract":"Aim: In this study, utilizing the in vivo Copenhagen rat model possessing prostate cancer, we studied the possible impact of tumorigenesis on testes and liver morphology and whether riluzole (RIL) and ranolazine (RNL) treatment would have any affect or not. Method: Male Copenhagen rats were divided into four groups: 1) Control group, 2) Cancer group, 3) Cancer + 10 μM Riluzole 4), and Cancer + 2.5 μM / 5 μM Ranolazine group. The tissue samples of testes and liver were taken and processed for light microscopy, including staining with hematoxylin and eosin. Results: In the cancer group, degenerated seminiferous tubules, cell remnants in the lumen were shown in the testis, and a decrease in the spermatogenic cell line was found. The deterioration in these parameters was milder in the treatment groups and an increase in the number of normal tubules was found. In the cancer group, pyknotic nucleus, mononuclear cell infiltration, hyperemia, vacuolization, disrupted arrangement of hepatocyte plates, sinusoidal dilatations, and degenerated hepatocytes were observed in the liver. However, there was a slight damage in cancer + 10 μM RIL, cancer + 2.5 μM RNL, and cancer + 5 μM RNL groups. Properly hepatocyte arrangement and sinusoidal enlargement were observed. Conclusions: This treatment can be considered a promising protective adjuvant candidate for testes and liver tissue in prostate cancer or cancer therapy-related damage.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122754269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bisphenol A levels in bowel endometrioma diagnosed serums: A case control study 肠子宫内膜瘤诊断血清中的双酚A水平:一项病例对照研究

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173849

Filiz Ardic, Humeyra Celik, H. Yeşilyurt, S. Otcu

引用次数: 0

The relationship of monocyte to high density lipoprotein-cholesterol ratio and complete blood count parameters with radiologic staging of knee osteoarthritis 单核细胞与高密度脂蛋白-胆固醇比值及全血细胞计数参数与膝骨关节炎放射学分期的关系

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173853

M. Yaşar, Elif Yakşi

{"title":"The relationship of monocyte to high density lipoprotein-cholesterol ratio and complete blood count parameters with radiologic staging of knee osteoarthritis","authors":"M. Yaşar, Elif Yakşi","doi":"10.30714/j-ebr.2022173853","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173853","url":null,"abstract":"Aim: To evaluate the predictive ability of bioindicators derived from complete blood count (CBC) parameters and monocyte-high density lipoprotein-cholesterol ratio (MHR) in the diagnosis of radiological stage of knee osteoarthritis (OA) in this study. Method: This cross-sectional retrospective study was carried out between November 2017 and June 2021, in our physical therapy and rehabilitation clinics. 65 patients knee x-rays and routine laboratory results were included in the study. Each knee x-rays were assigned a grade from 0 to 4 (Kellgren-Lawrence Classification System (KL). Patients were divided into two groups according to severity of the knee OA as follows. Group 1: Mild-moderate OA (KL Grade1-2), Group 2: Severe OA (KL Grade 3-4). Results: Independent T test and Mann Whitney U test were used to assess whether there was a difference in CBC parameters and their derivatives between two groups. Platelet lymphocyte ratio (PLR), red cell distribution width (RDW) to platelet ratio (RPR) and platelet (PLT) counts indicated statistically significant differences between the groups, p-values were 0.04, 0.03 and 0.04 respectively. There were no significant differences in terms of MHR score between the groups. Conclusions: We could not find a relationship between MHR and radiological degree of knee osteoarthritis. However, there is a correlation between radiological stage of knee osteoarthritis and hemogram parameters like PLT and their derivatives such as PLR and RPR.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116825922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the ATP-dependent K ( )-channel effectors pinacidil and glibenclamide on liver tissue in an experimental model of epilepsy: A histopathological study atp依赖性K通道效应剂pinacidil和格列本脲对实验性癫痫模型肝组织的影响:组织病理学研究

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173846

S. Duzcu, A. Çetinkaya, Hayriye Soyturk, Ş. Demir

引用次数: 0

Evaluation of the effect of intravenous ibuprofen use on postoperative pain and opioid consumption after abdominoplasty operation 静脉注射布洛芬对腹部成形术术后疼痛和阿片类药物消耗的影响

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173854

Mustafa Turkoglu, I. Yildiz, A. Gokkaya, Akın Dişikirik, A. Demirhan

{"title":"Evaluation of the effect of intravenous ibuprofen use on postoperative pain and opioid consumption after abdominoplasty operation","authors":"Mustafa Turkoglu, I. Yildiz, A. Gokkaya, Akın Dişikirik, A. Demirhan","doi":"10.30714/j-ebr.2022173854","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173854","url":null,"abstract":"Aim: Abdominoplasty is a common cosmetic procedure that is one of the most painful aesthetic surgery and has been used increasingly in recent years. Ibuprofen is a non-steroidal anti-inflammatory (NSAID) with antipyretic and analgesic effects. In this study, we aimed to evaluate the effectiveness of the intravenous (IV) form of ibuprofen on postoperative pain control and opioid requirement in patients who underwent abdominoplasty. Methods: The patients were divided into 3 groups as Group 1 (Tramadol), Group 2 (Ibuprofen) and Group 3 (Tramadol HCL + Ibuprofen). Tramadol HCL was given continuous infusion at a concentration of 4mg / ml via IV Patient Controlled Analgesia to Group 1. Ibuprofen 800 mg IV was administered to Group 2 at 30 minutes before the end of the operation. Patients were followed up by administering 800 mg IV every 6 hours for 24 hours. In Group 3, 30 minutes before the end of the operation, tramadol was administered via PCA with continuous infusion at a concentration of 4mg / ml and 800 mg IV ibuprofen was administered as 4x1. Results: VAS values were found to be significantly lower in Group 3 compared to Group 2 at every hour and at the 4th hour compared to Group 1. Group 3 was found to be significantly lower than Group 1 in total analgesic consumption in all time zones. Conclusion: We think that IV ibuprofen, which will be given in addition to tramadol after abdominoplasty, can provide effective analgesia and reduce analgesic consumption.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129037109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of silent cerebral ischemia and cognitive functions in young and middle- aged patients with metabolic syndrome 中青年代谢综合征患者无症状性脑缺血及认知功能的评价

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173858

Gulmine Dundar Yalcinkaya, S. Basaran

{"title":"Evaluation of silent cerebral ischemia and cognitive functions in young and middle- aged patients with metabolic syndrome","authors":"Gulmine Dundar Yalcinkaya, S. Basaran","doi":"10.30714/j-ebr.2022173858","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173858","url":null,"abstract":"Aim: Metabolic syndrome (MetS) risk factors and accompanying silent cerebral ischemia (SCI) may cause cognitive decline. The aim of this study is to investigate the characteristics and association between SCIs and cognitive functions in young and middleaged patients with MetS. Methods: A total of 91 patients who were aged between 20-60 years with a diagnosis of metabolic syndrome were prospectively enrolled. Silent cerebral infarcts (SCIs) were analyzed by performing 3 Tesla magnetic resonance imaging (MRI) of the brain. Attention and executive functioning were assessed with the Wechsler Adult Intelligent scale-Revised (WAIS-R), verbal fluency, Stroop, and clock drawing test. Wechsler Memory Scale-Revised (WMS-R) visual reproduction and Öktem verbal memory processes tests were conducted to determine visual and verbal memory performance, whereas visuospatial functions were evaluated by the Benton judgment of line orientation test. Results: Diagnosis of hypertension and diabetes mellitus were significantly higher, while educational level was lower in patients with SCIs than without SCIs. (p <0.001, p=0.034, p=0.05, respectively). Cognitive test scores regarding attention and executive functioning, verbal, visual, and visuospatial memory showed no significant difference between patients with SCIs and without SCIs. However, hyperhomocysteinemia was negatively correlated with executive functions in MetS patients with SCI (p= 0.038). Conclusions: SCIs may be associated with hypertension and diabetes mellitus in young and middle-aged patients with MetS. In addition, controlling homocysteine levels might be beneficial in MetS patients with SCI in terms of improving cognitive functions.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"22 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120906044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association between postoperative corneal edema and phacoemulsification level 术后角膜水肿与超声乳化水平的关系

Experimental Biomedical Research Pub Date : 2022-01-01 DOI: 10.30714/j-ebr.2022173855

Enes Uyar

{"title":"The association between postoperative corneal edema and phacoemulsification level","authors":"Enes Uyar","doi":"10.30714/j-ebr.2022173855","DOIUrl":"https://doi.org/10.30714/j-ebr.2022173855","url":null,"abstract":"Aim: To evaluate the effect of phacoemulsification level on postoperative central corneal thickness (CCT) differences and to assess the effects of demographic characteristics, biometric values and surgical parameters of patients on postoperative corneal edema. Methods: This prospective study included 232 eyes of 232 patients who underwent phacoemulsification surgery. Biometric values, phacoemulsification time, phacoemulsification power and effective phacoemulsification time (EPT) were evaluated. CCT measurements were repeated at follow-ups on days 1, 7 and 30 after surgery. Phaco-chop technique was performed in all patients and phacoemulsification level changes that occurred during surgery were recorded. Patients were grouped as Group 1: >75% of lens nucleus emulsified in capsular bag; Group 2: >75% of lens nucleus emulsified in iris plane; and Group 3: >50% of lens nucleus emulsified in anterior chamber. Results: Increase in the mean CCT values on the first day (incCCT1) and at the first week after surgery were more pronounced in Group 3 as compared with both Groups 1 and 2 (p < 0.05 for all). In addition, incCCT1 was higher in Group 2 than Group 1 (p=0.040). In the final model of multiple linear regression (R=0.31), it was determined that EPT, aqueous depth (AD) and phacoemulsification level significantly affected incCCT1 (p range: <0.001 to 0.009). Conclusions: The present study showed that more anterior phacoemulsification levels than the capsular bag caused higher CCT increase postoperatively. In addition, AD, EPT and phacoemulsification level were found as the most significant factors that influence postoperative corneal edema.","PeriodicalId":348987,"journal":{"name":"Experimental Biomedical Research","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130807212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1