mjlh klyh Tb lkndy最新文献

筛选
英文 中文
Thyroidectomy in elderly ; is it safe? 老年人甲状腺切除术;安全吗?
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.878
Mumtaz Khudhur Hanna Alnaser, Z. Kamal, W. Wardia, Bashar Hazim Basheer
{"title":"Thyroidectomy in elderly ; is it safe?","authors":"Mumtaz Khudhur Hanna Alnaser, Z. Kamal, W. Wardia, Bashar Hazim Basheer","doi":"10.47723/kcmj.v18i3.878","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.878","url":null,"abstract":"Background: The prevalence of thyroid nodules rise with age and different data available about the risks of thyroid surgery in old age people. In general, old age could be a predictor of perioperative mortality and morbidity. The aim of this study is to prove if there is increased risk accompanying thyroidectomy in elderly patients.\u0000Subjects and Methods: Prospective study of one surgeon of study sample undergoing thyroid surgery at Al-Kindy teaching hospital and Saint Raphael hospital .This study was including two groups; group (A) involved patients 65 years and older, group (B) involved patients below 65 years old who were subjected to thyroidectomy. Taking in consideration histopathology results, indications of surgery (compressive symptoms, suspicious or confirmed malignancy, toxic goiter and recurrent goiter) and complications (including rates of temporary and permanent hypocalcaemia, temporary and permanent RLN paralysis, postoperative hematoma, wound infection and seroma), in addition to the risk of perioperative mortality. \u0000Results: There were 574 patients below 65 years and 127 elderly patients (>=65 years) who underwent thyroidectomy between January 2015 and December of 2018. There were no deaths in either group; no one had bilateral RLN paralysis. Old age patients had a lower frequency of complications in comparison to the younger counterparts, including transient hypocalcaemia (3.1% vs 14.8%, respectively) and temporary RLN injury (0% vs 0.69%, respectively), in addition to permanent RLN injury (0% vs 0.34%, respectively).\u0000Conclusions: Thyroidectomy in elderly is safe as compared to younger patients regarding perioperative complications","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45053620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Association between CRP Levels with Comorbidities, Species, and Complications of Severe Malaria CRP水平与严重疟疾合并症、种类和并发症的关系
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.867
Salih Abdelwahab, Abdelsalam MA Nail, G. Modawe
{"title":"The Association between CRP Levels with Comorbidities, Species, and Complications of Severe Malaria","authors":"Salih Abdelwahab, Abdelsalam MA Nail, G. Modawe","doi":"10.47723/kcmj.v18i3.867","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.867","url":null,"abstract":"Background: Malaria remains a leading cause of mortality in sub-Saharan Africa (including Sudan). C-reactive protein (CRP) is useful as   a marker of severity in malaria. African studies have shown that serum CRP levels correlate with parasite burden and complications in malaria, especially falciparum. However, there are no data on CRP levels in Sudanese malaria patients.\u0000This study aims to evaluate the association between CRP levels with comorbidities, species, and complications of severe malaria\u0000Subjects and Methods: A cross-sectional study enrolled 65 severe malaria patients at Khartoum state hospitals during the period from April to June2021. Manifestations of severe malaria were defined according to WHO criteria. Data regarding demographics, presenting symptoms & signs, laboratory investigations, complications, length of hospital stay and outcomes were collected. CRP was classified as elevated when the measured level was >10 mg/l.\u0000Results: Among 65 patients, 33(50.8%) were females and 32(49.2%) were males, and mean age was 48±21 years. The main manifestation of severe malaria diagnosis criteria was anemia in 26(40%) patients. Most of the patients had density 1+ (n=53; 81.5%) and were infected by P. falciparum (n=61; 93.8%). The overall case fatality rate for malaria was 8% (n=15 patients). The mean of CRP was 72±57 mg/L and 84% (n=55) of patients had elevated levels above 10 mg/L (ranged from 10-100 mg/L in 52%, and above 100 mg/L in 32%). The elevated CRP levels were significantly DM (P= 0.048), high malaria parasite density in blood film (P= 0.001), P. falciparum (P= 0.33), presence of complications (P= 0.001) and death (P= 0.003)\u0000Conclusion: Elevated CRP levels were found in a considerable proportion of severe malaria patients. CRP is an effective biomarker in assessing malaria severity and poor prognosis in term of complications development and mortality.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49647954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pulmonary CT findings in Patients Recovered from COVID-19 Pneumonia COVID-19肺炎恢复期患者肺部CT表现
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.828
Sura Abass Fadhil, Muna Abdulghani
{"title":"Pulmonary CT findings in Patients Recovered from COVID-19 Pneumonia","authors":"Sura Abass Fadhil, Muna Abdulghani","doi":"10.47723/kcmj.v18i3.828","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.828","url":null,"abstract":"Background: The COVID-19 infection is a more recent pandemic disease all over the world and studying the pulmonary findings on survivors of this disease has lately commenced.\u0000Objective: We aimed to estimate the cumulative percentage of whole radiological resolution after 3 months from recovery and to define the residual chest CT findings and exploring the relevant affecting factors.\u0000Subjects and Methods: Patients who had been previously diagnosed with COVID-19 pneumonia confirmed by RT-PCR test and had radiological evidence of pulmonary involvement by Chest CT during the acute illness were included in the present study. The radiological data of chest CT scan of all patients were collected and analyzed after recovery (confirmed by negative RT- PCR) three months after their initial diagnosis of having COVID 19 pneumonia.\u0000Results: A total of 40 patients who had a second CT scans were assessed; there were 27 (67.5%) male and 13 (32.5%) female, with a mean age of 40.3 years old. The collective percentage of whole radiological resolution was 65% (26 patients). Patients >40 years old showed a significantly lower cumulative percentage of complete radiological resolution than patients ≤40 years old at the 3 months follow-up. The predominant patterns of abnormalities observed at discharge were ground-glass opacity (GGO), fibrous stripe and reticular opacities.\u0000Conclusion: Lung findings in COVID-19 pneumonia patients can be resolved completely during medium-term follow up with no sequelae. The older age and co-morbidities are the main significant risk factors for residual radiological findings of COVID-19 disease.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41397563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changing the Trends in Surgery during the COVID-19 Times: An Experience from the Eastern Uttar Pradesh State, India 新冠肺炎时期外科手术趋势的变化:印度北方邦东部的经验
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.870
P. Pandey, S. Bharat, Ashutosh Gupta
{"title":"Changing the Trends in Surgery during the COVID-19 Times: An Experience from the Eastern Uttar Pradesh State, India","authors":"P. Pandey, S. Bharat, Ashutosh Gupta","doi":"10.47723/kcmj.v18i3.870","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.870","url":null,"abstract":"Background: The COVID-19 pandemic has an immense effect not only on the social and economic lives of people but also on the surgical lives of surgeons, residents, nursing staff, and patients as well as ground level staff. Amidst this COVID pandemic, emergency surgeries were being done but at a decreased rate, whereas elective cases depended on the will of hospitals, surgeons, and patients. Study aims to promulgate a \"Neo–Surgical Check Box\" by amalgamating the WHO surgical checklist and the results obtained from the questionnaires.\u0000Subjects and Methods: After receiving ethical clearance from the Institute Ethical Committee, an online questionnaire with 50 questions divided into three sections was distributed to 235 surgeons in Uttar Pradesh.\u0000Results:Two hundred and eight surgeons had responded, out of which 154 were male and 54 were female. Only 41.4% (86) were actually conducting the surgery. The emergency surgery rate was 60.3%, whereas 18.6% of elective surgeries were done, 11.8% of surgeries were avoided, and the rate of minimal access surgery was 9.3%.\u0000Conclusion: The \"Neo-Surgical Check Box\" will not only increase the number of surgeries per day but also provide protection to the healthcare workers while handling not only COVID-positive patients but also any airborne communicable diseases.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45923472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Microbiology in Clinical Practice: Current and Future Applications 分子微生物学在临床实践中的应用现状与展望
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i2.857
Tracy Biney-Assan, M. Kron
{"title":"Molecular Microbiology in Clinical Practice: Current and Future Applications","authors":"Tracy Biney-Assan, M. Kron","doi":"10.47723/kcmj.v18i2.857","DOIUrl":"https://doi.org/10.47723/kcmj.v18i2.857","url":null,"abstract":"Technological advances have yielded new molecular biology-based methods for the diagnosis of infectious diseases.  The newest and most powerful molecular diagnostic tests are available at regional and national reference laboratories, as well as at specialized centers that are certified to conduct metagenomic testing.  Metagenomic assays utilize advances in DNA extraction technology, DNA sequence library construction, high throughput DNA sequencing and automated data analysis to identify millions of individual strands of DNA extracted from clinical samples.  At present, metagenomic assays are only possible at a small number of special research, academic and commercial laboratories.  Continued research in human and pathogen genomic organization and host-pathogen interactions, represent important future goals that will maximize the information obtained from metagenomic assays.  To illustrate the power and limitations of metagenomics, we report on a previously healthy 27 year old woman with work related exposure to ill animals, and who developed a rapidly progressive, severe diffuse interstitial pneumonitis that ultimately ended in the need for a double lung transplant.   Metagenomic testing on DNA extracted from pleural fluid and nasopharyngeal swabs demonstrated the presence of expected normal bacterial flora along with some unexpected herpesvirus and non-HIV retroviral elements integrated into the patients DNA.  Although no specific pathogen was ultimately identified to explain this patient’s severe disease, the sample preparation and data analysis methods detailed herein illustrate the powerful benefits and limitations of metagenomic testing.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47516227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gingival Pigmentation Pattern in Correlation to Skin Color in a Group of Kurdish People in Sulaimani City 苏莱曼尼市一群库尔德人的牙龈色素沉着模式与肤色的关系
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.874
S. Amin, S. H. Azeez
{"title":"Gingival Pigmentation Pattern in Correlation to Skin Color in a Group of Kurdish People in Sulaimani City","authors":"S. Amin, S. H. Azeez","doi":"10.47723/kcmj.v18i3.874","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.874","url":null,"abstract":"Background: Melanin pigmentation of the gingiva appears in all ethnicities. Excessive pigmentation is an esthetic concern that has increased awareness about depigmentation procedures. This epidemiological study aims to find the correlation between skin color and gingival pigmentation in Sulaimani Governorate, Kurdistan/Iraq.\u0000Subjects and Methods: A total of 820 apparently healthy and non-smokers, including 338 males and 482 females with healthy gingiva, aged between (18-40 years old) were enrolled in this study. Clinical examination on the participants’ gingivae was performed to assess color, and the distribution of pigmentations. Afterward the of participants skin color were visually examined and divided into six groups according to the Fitzpatrick scale.\u0000Results: Skin color of (53%) of our participants was medium to light brown. Most of the pigmentations were observed in the attached gingiva and interdental papilla (26.83%) and least being in the marginal gingiva and interdental papilla (9.27%). A statistically significant correlation was found between the participants’ skin color, and the intensity of their gingival pigmentations  \u0000Conclusion: Darker-skinned subjects haveeavieringival pigmentation compared toair-skinned people in Sulaimani Governorate, Kurdistan/ Iraq. The intensity of gingival pigmentation increases with age with the highest rate of gingival pigmentation was in the upper and lower second premolar to premolar area.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49244606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative study on Various Tube Voltages and Contrast Media Doses in CT Pulmonary Angiography to detect Pulmonary Thromboembolism 不同管电压和造影剂剂量在CT肺血管造影中检测肺血栓栓塞的比较研究
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.808
Shaymaa Khalid Abdul-Qader, A. A. Fadhil
{"title":"Comparative study on Various Tube Voltages and Contrast Media Doses in CT Pulmonary Angiography to detect Pulmonary Thromboembolism","authors":"Shaymaa Khalid Abdul-Qader, A. A. Fadhil","doi":"10.47723/kcmj.v18i3.808","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.808","url":null,"abstract":"Background: Lowering the amount of iodinated contrast material and tube voltage may increase pulmonary artery opacification and thrombus identification without compromising picture quality.\u0000Objectives: To explore the efficiency of using lower tube voltage and a lower contrast medium dose for conducting computed tomography for pulmonary angiography (CTPA) aiming to increase its accuracy in detecting pulmonary thromboembolism (PTE).\u0000Subjects and Methods:100 patients scheduled for CTPA with a preoperative diagnosis of PTE were grouped into two: group A, (50 patients) got 1 mL/kg at 120 kV and group B, (50 patients) received 0.5 mL/kg at 80 kV.The technique of bolus tracking was implemented.Values of midpoint of multiple pulmonary artery divisions were used to assess attenuation.Significant values are those that exceed 300 Hounsfield units (HU). The dosage of radiation received by the patient, defined as the effective dose, was compared between groups.\u0000Results: Total mean attenuationdata for the pulmonary arteries have shown to be substantially greater in group-B compared to group-A (39110.4HU vs. 335.97.9HU, P=0.001). Moreover, total dose length product (DLP) values were substantially lower in group-B (934.9 mGy.cm vs. 384.443 mGy.cm, P=0.001). While effective dose values have shown to be significantly lower in group-B (2.90.3 mSv vs. 13.21.8 mSv, P 0.001).\u0000Conclusion: Low-kilovoltage technique and low quantity of iodinated contrast are useful in conducting (CTPA) with high accuracy in detection of pulmonary thromboembolism.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41364253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lifestyle Medicine: A Promising tool to Restoring Health 生活方式医学:恢复健康的有希望的工具
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.932
E. Hameed, Laith Thamer Al-Ameri
{"title":"Lifestyle Medicine: A Promising tool to Restoring Health","authors":"E. Hameed, Laith Thamer Al-Ameri","doi":"10.47723/kcmj.v18i3.932","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.932","url":null,"abstract":"Lifestyle Medicine is the application of evidence-based lifestyle approaches for the prevention, treatment, and even the reversal of lifestyle-related chronic diseases such as diabetes, hypertension, heart disease, obesity, polycystic ovarian diseases, dementia, arthritis, and cancers","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44405758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Efficiency of Corn Solution as a Cytological Fixative in Buccal Smear 玉米溶液作为口腔涂片细胞学固定剂的效果
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.839
Jalal Eldein Mahmoud Nour Wara, Mohammed Abdulelah Abuzeid Abdulrahem, Tasabeeh Mustafa Alzain Salem, Waad Akram Albager Mohammed, Walaa Tamim Eldar Khalaf Allah Abuzeid, Maha Hamid Omer Bushara, Amna Mohammed Hassan, A. Idris
{"title":"The Efficiency of Corn Solution as a Cytological Fixative in Buccal Smear","authors":"Jalal Eldein Mahmoud Nour Wara, Mohammed Abdulelah Abuzeid Abdulrahem, Tasabeeh Mustafa Alzain Salem, Waad Akram Albager Mohammed, Walaa Tamim Eldar Khalaf Allah Abuzeid, Maha Hamid Omer Bushara, Amna Mohammed Hassan, A. Idris","doi":"10.47723/kcmj.v18i3.839","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.839","url":null,"abstract":"Background: Corn Syrup is food syrup higher of carbohydrate, depending on grade. The study aimed to assess efficiency of Corn syrup as cytological fixative.\u0000Subjects and methods: This was laboratory based study, it has been conducted at Elrazi University included   apparently 30 healthy students have been involved in this study.\u0000Results: Out of 30 smears fixed with 95% alcohol, 76.7% (n=23) shows excellent nuclear stain, 23.3% (n= 7)   shows good nuclear stain. 70% (n=21) show excellent cytoplasmic stain, 26.7% (n=8) shows good cytoplasmic stain, 3.3% (n=1) shows poor cytoplasmuc stain.\u0000   Out of 30 smears fixed with corn solution, 60% (n=18) shows excellent nuclear stain, 40% (n=12) good nuclear stain, 3.3 % (n=1) shows excellent cytoplasmic stain, 83.3% (n=25) shows good cytoplasmic stain, 13.3% (n=4) shows bad cytoplasmic stain.\u0000Conclusion: Study concluded that Corn syrup can be used as cytological fixative alternatively to 95% ethyl alcohol.","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42888915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Streptocin SH3, a Bacteriocin produced by Streptococcus sanguinis isolated from Human Dental Plaque 从人牙菌斑中分离的血链球菌产生的一种细菌素——链球菌SH3的评价
mjlh klyh Tb lkndy Pub Date : 2022-12-31 DOI: 10.47723/kcmj.v18i3.843
Shahad Fadhil Al-Taie, Muna T. Al- Musawi, Zaid S. Rasheed
{"title":"Evaluation of Streptocin SH3, a Bacteriocin produced by Streptococcus sanguinis isolated from Human Dental Plaque","authors":"Shahad Fadhil Al-Taie, Muna T. Al- Musawi, Zaid S. Rasheed","doi":"10.47723/kcmj.v18i3.843","DOIUrl":"https://doi.org/10.47723/kcmj.v18i3.843","url":null,"abstract":"Background: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity. Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries. \u0000Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined.  After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography using Sephadex G-50 column. Finally, physical characteristics were also studied and determined the bacteriocin stability.\u0000Results: Among all streptococcal isolates, the S. sanguinis SH3 isolate with batter antimicrobial activity was selected, and used in further experiments. The optimum conditions for bacteriocin production were in Todd Hewitt broth (THB) supplemented with 1% glucose at 37°C for 24h under anaerobic conditions and inoculum size of (1.2 ˟ 109CFU/ml) and pH 7, where the inhibition zone diameter reached to 29 mm against Enterococcus faecalis. The purified bacteriocin had high inhibitory activity against S. pneumoniae, S. pyogenes, E. faecalis, E. faecium and  Leuconostoc mesenteroides. Streptocin SH3 keeps its effectiveness within the pH range 3-10, and temperature until 80ºC for 20 min.\u0000Conclusion: Bacteriocin produced from S. sanguinis has high activity against many pathogens and has high stability over wide ranges of temperature and pH, which makes it a good alternative in the medical and food preservation fields","PeriodicalId":34748,"journal":{"name":"mjlh klyh Tb lkndy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44619294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信