Journal of the International Society for Plastination最新文献

Chicken plastination: its role in teaching avian anatomy 鸡塑化:在禽类解剖学教学中的作用

Journal of the International Society for Plastination Pub Date : 2023-10-14 DOI: 10.56507/gxlg9124

{"title":"Chicken plastination: its role in teaching avian anatomy","authors":"","doi":"10.56507/gxlg9124","DOIUrl":"https://doi.org/10.56507/gxlg9124","url":null,"abstract":"This paper describes the preparation of six plastinated chicken dissections for Veterinary Anatomy teaching at the University of Pretoria, South Africa. Specimens were fixed in 10% formalin for seven to ten days, depending on the size of the specimen. Room temperature acetone baths were used for dehydration over a period of six weeks. Impregnation in S10 took place over a period of three weeks at -16 °C. The specimens were cured with S6. A peristaltic pump was used during preparation to ensure the intestines were rinsed properly, and the chemicals penetrated the whole specimen. In the curing phase, a compressor was used to inflate the duodenum and small intestines to show the digestive and reproductive tracts. The plastinated specimens were then labelled. Three chicken hearts in different stages of dissection (one intact, one from the ventrum/cranial, one from the dorsum/caudal aspect) were prepared. A muscle dissection was carried out on a whole chicken with its intestines intact. It was found to be preferable to carry out the dissection while the specimen was stored in the acetone bath. When the dissection was complete, the body wall of the chicken was cut through with an oscillating saw before impregnation in S10. Another adult chicken was used for the skeleton. All the muscle was removed, and the skeleton placed in formalin for fixation. After a week, the skeleton was placed in acetone for dehydration. The aim with this skeleton was to show how the ligaments maintain skeletal integrity. The ovary and reproductive tract were also dissected. The plastinated specimens are now available to the students in the anatomical museum. Now students, at their own pace, may study and learn the anatomy of the chicken more intensely.","PeriodicalId":343741,"journal":{"name":"Journal of the International Society for Plastination","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A digital resource based on plastinated specimens for learning dilated cardiomyopathy in dogs 基于塑化标本的犬扩张型心肌病学习数字资源

Journal of the International Society for Plastination Pub Date : 2023-10-14 DOI: 10.56507/opvs3701

{"title":"A digital resource based on plastinated specimens for learning dilated cardiomyopathy in dogs","authors":"","doi":"10.56507/opvs3701","DOIUrl":"https://doi.org/10.56507/opvs3701","url":null,"abstract":"In this study, plastinated specimens were used for creating a learning model to assist students’ understanding of a common cardiac pathology of dogs. Dilated cardiomyopathy is a myocardial disease, causing a progressive dilatation of all four cardiac chambers, with concomitant changes in systolic function. Two plastinated sections of canine hearts were used, one from a healthy dog, and the other belonging to a dog affected by dilated cardiomyopathy. Plastinated sections were photographed and digitized, and then a static image animation program was used to simulate the heartbeat, in a reliable manner. The digital materials were used to create a video tutorial, in which the structures of the heart were simultaneously highlighted in both anatomical and ultrasound images. The video tutorial was accessed via QR code, which was given to a set of students of the Veterinary Degree Program with previous basic training in ultrasound diagnosis. A satisfaction survey was used to monitor each student's perception of the importance of clinical anatomy and the usage and learning experience with the video tutorial. The results reported that most students considered anatomy as a fundamental subject for their professional career, although their knowledge about it at the end of the degree was not too extensive. The overall score of the video tutorial was very high, as it apparently facilitated the understanding of ultrasound imaging related to dilated cardiomyopathy. It is concluded that the combined use of plastinated material with e-learning improved the subjective perception of learning and understanding of the dilated cardiomyopathy by the students.","PeriodicalId":343741,"journal":{"name":"Journal of the International Society for Plastination","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different traditional methods of cleaning skeletal material: Preliminary evaluation based on scanning electron microscopy 不同传统方法清洗骨骼材料的效果:基于扫描电镜的初步评价

Journal of the International Society for Plastination Pub Date : 2023-10-14 DOI: 10.56507/ffwh1619

{"title":"Effects of different traditional methods of cleaning skeletal material: Preliminary evaluation based on scanning electron microscopy","authors":"","doi":"10.56507/ffwh1619","DOIUrl":"https://doi.org/10.56507/ffwh1619","url":null,"abstract":"Biological collections are unique repositories of biodiversity. Ideally, institutions should have standardized protocols for preparation, storage, and conservation of materials, designed to minimize deterioration over time and to ensure that comparable results could be obtained from them. Eleven cleaning treatments, frequently used in scientific collections, were performed on Wistar rat femurs, consisting of burial (60 days), and enzymatic and chemical digestion. For the last two techniques, ten combinations of concentration of the agents (enzymes, potassium hydroxide [KOH]), temperature, and exposure time were tested. After treatment, bone integrity and percentage of surface covered by soft tissues were evaluated using images obtained by scanning electron microscopy. Good results, in terms of cleaning parameters (muscle and fat removal) were obtained with burial and with the KOH 10%/40 °C/2h and KOH 5%/40 °C/4h combinations; however, superficial desquamation, cracking, and porosity (parameters of bone surface damage) were observed in all cases. Other KOH combinations seemed to be less efficient to clean the surface, but the bones were better preserved. In enzymatic treatments, bone integrity was less affected but more residues persisted; the amount of tissue remaining appears to be related to temperature (treatments at 70 °C were more effective than at 25 °C). Damage caused by burial and KOH coincided with that observed by other authors, although enzymatic treatments left greater amounts of tissue than previously reported. The preliminary information gathered provides a starting point to implement conservative cleaning of skeletal material and will surely constitute an important advance for the establishment of protocols in biological collections.","PeriodicalId":343741,"journal":{"name":"Journal of the International Society for Plastination","volume":"234 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetone recycling: Do molecular sieves cause acetone breakdown to acetaldehyde? 丙酮回收:分子筛会导致丙酮分解为乙醛吗?

Journal of the International Society for Plastination Pub Date : 2023-10-14 DOI: 10.56507/qisd7419

{"title":"Acetone recycling: Do molecular sieves cause acetone breakdown to acetaldehyde?","authors":"","doi":"10.56507/qisd7419","DOIUrl":"https://doi.org/10.56507/qisd7419","url":null,"abstract":"Plastination is a process that is used to preserve biological tissue. In the plastination process, one of the major steps involves dehydrating the tissue with acetone. During this process, large amounts of acetone are used. In order to reduce lab costs, acetone is routinely recycled. This process uses a commercial acetone recycler to remove the majority of impurities that are present in acetone after the dehydration process. Once recycled, the acetone is exposed to molecular sieves in order to remove the water from the acetone. Molecular sieves work by binding to the smaller molecules (water) as the acetone molecules are exposed to them. When exposed to the sieves, the recycled acetone can reach a purity level approaching 100%. The use of molecular sieves in acetone recycling is thought to change the chemical makeup of acetone to acetaldehyde. Acetaldehyde is more reactive than acetone because it has less steric hindrance with the hydrogen compared to the methyl group. Acetaldehyde has a lower melting point (-120℃) and boiling point (20℃) than acetone (Mp -95℃ and Bp 56℃). Because of this phenomenon, it has been recommended that molecular sieves should not be used to recycle acetone. In order to observe the veracity of this assertion, we utilized NMR (nuclear magnetic resonance) to analyze the recycled acetone for the presence of acetaldehyde. The samples used included pure (new) acetone, used acetone before recycling, used acetone after recycling, and used acetone after molecular sieves. From the preliminary and subsequent NMR spectra studies, there were no aldehyde groups present in any of the sample groups. Based on our NMR results, the use of molecular sieves does not appear to alter the chemical makeup of the acetone during the recycling and purification process. Since acetone is not affected by this process, we conclude that the use of molecular sieves is an effective and cost-saving method of acetone recycling for its use in the plastination process.","PeriodicalId":343741,"journal":{"name":"Journal of the International Society for Plastination","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curing Times of P40 Exposed to Different Light Sources P40在不同光源下的固化时间

Journal of the International Society for Plastination Pub Date : 2008-07-31 DOI: 10.56507/vgpg1291

R. Reed, L. Helms

引用次数: 0

Silicone Plastination Procedure for Producing Thin, Semi- transparent Tissue Slices: A Study Using the Pig Kidney 用猪肾制备薄的半透明组织片的硅酮塑化工艺研究

Journal of the International Society for Plastination Pub Date : 2008-07-31 DOI: 10.56507/jdqp3855

L. Pendovski, V. Petkov, F. Popovska-Perčinić, V. Ilieski, Lazar Pop Trajkov

引用次数: 6

Evaluation of Imidazole for Color Reactivation of Pathological Specimens of Domestic Animals 咪唑对家畜病理标本颜色恢复作用的评价

Journal of the International Society for Plastination Pub Date : 2008-07-31 DOI: 10.56507/hztr8339

B. Méndez, R. Romero, F. Trigo, R. Henry, A. Candanosa

引用次数: 5

Acetone Discoloration of Epoxy Reaction-Mixture 环氧树脂反应混合物的丙酮变色

Journal of the International Society for Plastination Pub Date : 2008-07-31 DOI: 10.56507/udsz2853

R. Reed, R. J. Whaley

引用次数: 0

Silicone Plastination of Biological Tissue: Cold-temperature Technique Biodur© S10/S15 Technique and Products 生物组织硅酮塑化:低温技术Biodur©S10/S15技术和产品

Journal of the International Society for Plastination Pub Date : 2007-07-31 DOI: 10.56507/zlmj7068

K. DeJong, R. Henry

引用次数: 65

Polyester Plastination of Biological Tissue: P35 Technique 生物组织聚酯塑化:P35技术

Journal of the International Society for Plastination Pub Date : 2007-07-31 DOI: 10.56507/mfed4472

W. Weber, A. Weiglein, R. Latorre, R. Henry

引用次数: 15