Nepal Journal of Biotechnology最新文献_第5页

Thyroid Function and Thyroglobulin Level in Iodine-Deficient Children of Eastern Nepal 尼泊尔东部缺碘儿童的甲状腺功能和甲状腺球蛋白水平

Nepal Journal of Biotechnology Pub Date : 2020-04-23 DOI: 10.21203/rs.3.rs-24159/v1

S. Kunwar, S. Khatiwada, B. Gelal, S. Thapa, G. Shah, N. Baral, M. Lamsal

{"title":"Thyroid Function and Thyroglobulin Level in Iodine-Deficient Children of Eastern Nepal","authors":"S. Kunwar, S. Khatiwada, B. Gelal, S. Thapa, G. Shah, N. Baral, M. Lamsal","doi":"10.21203/rs.3.rs-24159/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-24159/v1","url":null,"abstract":"Iodine deficiency during childhood affects physical and mental development. Iodine deficiency or excess both can negatively impact thyroid function. We conducted this study to assess iodine nutrition and thyroid function in children with insufficient urinary iodine concentration. A community-based cross-sectional study was conducted among the selected schools of Udayapur district. Urinary iodine concentration (UIC) was measured in 1012 school children (6-14 years). Based on UIC data, 83 blood samples were collected to measure serum thyroglobulin (Tg), thyroid-stimulating hormone (TSH), free triiodothyronine (fT3), and free thyroxine (fT4). UIC was measured by ammonium persulfate digestion method, and Tg, TSH, fT4, and fT3 were measured using ELISA kits. The median UIE was 236 µg/L, and 11.1% of the children had insufficient UIC. The mean fT3, fT4, and TSH in children with insufficient UIC were 2.55±0.43 pg/mL, 0.96±0.28 ng/dL, and 3.60±1.44 mIU/L respectively. Among children with low UIC levels, the median Tg was 17.5 ng/mL. Overt hypothyroidism was seen in 6%, and subclinical hypothyroidism in 3.6%. The children had sufficient iodine nutrition, and the frequency of thyroid dysfunction was low among the children with insufficient UIC.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88518558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana 美洲龙舌兰的植物化学、抗菌、抗氧化活性和细胞毒性评价

Nepal Journal of Biotechnology Pub Date : 2019-12-29 DOI: 10.3126/njb.v7i1.26948

B. R. Pandey, Angela Shrestha, N. Sharma, B. Shrestha

{"title":"Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana","authors":"B. R. Pandey, Angela Shrestha, N. Sharma, B. Shrestha","doi":"10.3126/njb.v7i1.26948","DOIUrl":"https://doi.org/10.3126/njb.v7i1.26948","url":null,"abstract":"Ethnomedicinal plants are being used as a source of medicine from ancient time but they lack the proof of modern scientific evidence for their effectiveness. This study focuses on the evaluation of phytochemical, antimicrobial, antioxidant properties of one of the ethnomedicinal plant Agave americana from Dhulikhel region of Nepal. The plant extract was prepared using solvent-based warm soxhlet extraction from the leaves of the plant and antimicrobial activity against six different non-resistant clinical isolates of bacteria (Staphylococcus aureus, Shigella, Klebsiella pneumoniae, Escherichia coli, Bacillus thuringiensis, and Salmonella paratyphi) was evaluated using agar disc diffusion method along with qualitative analysis for presence/absence of phytochemicals. Antioxidant activity was measured by DPPH assay and the cytotoxicity was evaluated using MCF-7 (human breast adenocarcinoma) cancer cell. Presence of phytochemicals like alkaloids, flavonoids, reducing sugars and saponins were detected in the plant extract. The extract was found to show some level of antimicrobial activity against Staphylococcus aureus, Escherichia coli and Bacillus thuringiensis at 50, 100 and 200 mg/ml. The IC50 value of the extract was found to be 7.68 μg/ml. The extracts of Agave americana showed 50 % cell-death of MCF-7 in 12 h at 5 μg/ml. Although this study provided some scientific evidence for the medicinal value of Agave americana, further studies are still needed for the detailed evaluations of every molecule present in this plant along with screening in larger geographical area of Nepal.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83982963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Isolation and Screening of Antibiotics Producing Streptomyces spp from the Soil Collected around the Root of Alnus nepalensis from Godawari 戈达瓦里尼泊尔桤木根周土壤产抗生素链霉菌的分离与筛选

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22337

A. Baniya, Sushma Singh, Minu Singh, Pragya Nepal, M. Adhikari, S. Aryal, A. Adhikari

{"title":"Isolation and Screening of Antibiotics Producing Streptomyces spp from the Soil Collected around the Root of Alnus nepalensis from Godawari","authors":"A. Baniya, Sushma Singh, Minu Singh, Pragya Nepal, M. Adhikari, S. Aryal, A. Adhikari","doi":"10.3126/NJB.V6I1.22337","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22337","url":null,"abstract":"Actinomycetes are considered as the most invaluable prokaryotes whose genome mining show a great number of putative secondary metabolite biosynthesis pathways as well as gene clusters ranging from 20 to 50 per genome. The genus Streptomyces has been explored for its ability to produce 60% antibiotics worldwide. Alnus nepalensis (Alder) has been found to harbor diverse Eubacteria in its rhizosphere. To evaluate the antibiotic production potential from Actinomycetes, we collected soil samples from rhizosphere (5-7 cm deep) of Alder tree. Primary screening was done by cross-streak method against Multidrug Resistant (MDR) such as Methicillin resistant Staphylococcus auereus (MRSA), Vancomycin resistant Enterococcus feacalis (VRE), Imepenem resistant Acinetobacterbaumannii, Vancomycin resistant Klebsiella pneumonia and Imepenem resistant E. coli as well as Non-MDRs (E. coli, Bacillus subtilis, Klebsiella pneumoniae, S. aeureu and Enterococcus feacalis). Extraction of antibiotics was done using rota-vapour from extract obtained by solid-substrate fermentation technique followed by solvent extraction. Secondary screening was done using well diffusion assay against MDRs. Among total of 40 isolates of Actinomycetes recovered, 14 showed remarkable zone of inhibition (ZOI) to various MDRs. NASA 303 showed 26 mm of ZOI against VRE, NASA 101 had ZOI of 34 mm against MRSA, NASA 319 had 33.7 mm ZOI against Imepenem resistant E. coli, NASA 306 had 36 mm of ZOI against Vancomycin resistant Klebsiella pneumoniae, and NASA 108 showed ZOI of 29.6 mm against Imepenem resistant E. faecalis. This investigation revealed that the Actinomycetes found in Rhizosphere of Alder tree had MDR killing potent antibiotics, which needs to be further explored.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86089623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

In Vitro Comparative Study of Antioxidant and Antibacterial Activity of Selected Dietary Plants 选定膳食植物抗氧化和抗菌活性的体外比较研究

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22336

Ashok Kumar, Palvi Sharma, A. Mahajan, Z. Begum

引用次数: 5

Development of Cost Optimized Horizontal Gel Electrophoresis Running Unit for Developing Countries (Nepal) 面向发展中国家的成本优化水平凝胶电泳运行装置的研制(尼泊尔)

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22333

Sajesan Aryal, Aroj Hada, S. Hamal, Abhishek Prajapati, Paras Mani Timilsina, S. Adhikari

{"title":"Development of Cost Optimized Horizontal Gel Electrophoresis Running Unit for Developing Countries (Nepal)","authors":"Sajesan Aryal, Aroj Hada, S. Hamal, Abhishek Prajapati, Paras Mani Timilsina, S. Adhikari","doi":"10.3126/NJB.V6I1.22333","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22333","url":null,"abstract":"The use of expensive lab techniques has left many high schools and even university students unacquainted with the basic experimental procedures and protocols in developing country including Nepal. Horizontal gel electrophoresis is one of the expensive protocols, which every student in the laboratory may not get an equal chance to access individually. However, this technique, being indispensable and inevitable in molecular biology principles, is of abounding importance for students to be familiar with. Thus, realizing its importance, we present an extremely simple and inexpensive design of gel-electrophoresis unit, which emulates electrophoresis analysis with the use of nichrome and aluminum wires as a substitute for platinum wires, together with daily used plastic materials. Because of these factors, the approximate cost of unit design is significantly reduced to an amount of 10 USD. The efficiency of the substitute wires was confirmed and it resulted in satisfactory data characterized by good resolution of the DNA fragments. The inexpensive nature, good results and simplicity of the device make it an ideal unit for teaching and learning in developing countries.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"196 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77083622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative Antioxidant, Antimicrobial and Phytochemical Assesments of Leaves of Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f.

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22331

Pragya Nepal, Minu Singh, A. Baniya, Sushma Singh, Hari Krishna Sainju, R. Shrestha

{"title":"Comparative Antioxidant, Antimicrobial and Phytochemical Assesments of Leaves of Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f.","authors":"Pragya Nepal, Minu Singh, A. Baniya, Sushma Singh, Hari Krishna Sainju, R. Shrestha","doi":"10.3126/NJB.V6I1.22331","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22331","url":null,"abstract":" Nepal is rich in varieties of religious plants. The locally used religious plants also carry medicinal importance. Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f. are three plants belonging to the family Poaceae having religious significance in different practices of Hinduism. They were also used as traditional medicines by our ancestors but nowadays they are underutilized. In this research, our core objective was to validate the traditional assumption of use of these plants in medicinal purposes by carrying out the assessments like antimicrobial assessment, antioxidative assessment and phytochemical assessment. Methanolic extracts produced from leaves of all three plants were examined for antimicrobial activities through agar well diffusion method. The same extracts were also assessed for determining their antioxidative potentials with the use of DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical scavenging assay followed by qualitative phytochemical analysis and GCMS (Gas Chromatography Mass Spectroscopy). Most promising antimicrobial activity was shown by Desmostachya bipinnata L. against Salmonella typhimurium and Staphylococcus aureus, Drepanostachyum falcatum (Nees) Keng f. against Salmonella typhimurium and Klebsiella pneumoniae and Hordeum vulgare L. against Salmonella typhmurium and Staphylococcus aureus. The antioxidant activity of the plant extracts were observed in descending order of Hordeum vulgare L.>Desmotachya bipinnata L. > Drepanostachyum falcatum (Nees) keng f. and phytochemical assessment of the extracts indicated the presence of alkaloids, glycosides, sterols, Triterpenes, Saponins, Flavonoids, Coumarins, Phlobatanin and reducing sugars. Through this project, we can clarify that the above mentioned plants have bioactive compounds which contributed for the presence of antimicrobial and antioxidative property in the plants.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90397689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods 两种泡桐生长统计的比较及生根方法的优化

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22330

H. K. Saiju, Abhishesh Bajracharya, Brishav Rajbahak, S. Ghimire

引用次数: 2

Fecal carriage of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella spp. among School Children in Pokhara, Nepal 产大肠杆菌和克雷伯氏菌的广谱β-内酰胺酶(ESBL)在尼泊尔博卡拉学龄儿童中的粪便携带情况

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22334

B. Baral, B. Sharma, K. Gurung, R. Gurung, H. S. Supram, B. Bhattachan

{"title":"Fecal carriage of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella spp. among School Children in Pokhara, Nepal","authors":"B. Baral, B. Sharma, K. Gurung, R. Gurung, H. S. Supram, B. Bhattachan","doi":"10.3126/NJB.V6I1.22334","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22334","url":null,"abstract":"Extended-spectrum β-lactamases (ESBL) producing microbes in recent years have been a major problem in developing countries like Nepal, with limited treatment options. This study aimed to determine the prevalence of ESBL producing E. coli and Klebsiella spp. in school children in Pokhara, Nepal. The study was conducted from June to October, 2015 at the microbiology laboratory of Manipal Teaching Hospital, Pokhara, Nepal. Antibiotic Susceptibility Test (AST) was done after isolation and identification of bacterial isolates. Then, presence of ESBL enzymes in E. coli and Klebsiella spp. were tested by combination disc diffusion test using cefotaxime and ceftazidime alone and with clavulanic acid. Out of total 309 school children, 211 (68%) bacterial isolates were detected from stool samples. Among them, E. coli and Klebsiella spp. were detected in 97 (46%) and 39 (19%) stool samples respectively. Bacteria isolated from 14 (5%) stool samples were multi-drug resistant (MDR) positive. After applying combined disk method, 88 (29%) isolates were found to be ESBL producer. Emerging prevalence rate of ESBL producing E. coli and Klebsiella spp. are major problem in medical history. Therefore, rapid need of surveillance for effective management of such MDR-strain is required.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83944577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Isolation of Bacillus spp. Bacteria from Soil for Production of Cellulase 从土壤中分离生产纤维素酶的芽孢杆菌

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22338

Amika Ahmed Manzum, A. A. Mamun

{"title":"Isolation of Bacillus spp. Bacteria from Soil for Production of Cellulase","authors":"Amika Ahmed Manzum, A. A. Mamun","doi":"10.3126/NJB.V6I1.22338","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22338","url":null,"abstract":"Cellualse is one of the most important enzymes used in textile, detergent, paper, food and feed industries. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples. 24 soil samples were analyzed and 54 presumptive Bacillus isolates were isolated after heating the soil samples at 80°C for 10 min. Among them 45 isolates showed enzyme activity ranging from 0.003 to 0.17 U/ml in test tubes containing 5 ml medium composed of (g/L) glucose 0.5 gm, peptone 0.75 gm, FeSO4 0.01 gm, KH2PO4 0.5 gm, and MgSO4 0.5 gm at 120 rpm, 37° C and pH 7. Among them 1RW, 2WS, 3YR, 4WT, 6 RR, and 9SS showed 0.17, 0.15, 0.14, 0.15, 0.147 and 0.14U/ml enzyme activities, respectively. Production of cellulase by these isolates was further scaled up to shake culture containing 50 ml medium similar to that used in test tube culture. Among the isolates 1 RW showed the maximum activity. This 1 RW was identified by API kit and showed that 59 % belongs to Bacillus licheniformis strain (51% confirmation) or Bacillus subtilis (31% confirmation). Further gene analysis is required to confirm the species. The genetic improvement study will make the isolate a good source of cellulase.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"58 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75390908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors 尼泊尔博卡拉输血中心献血者中乙型和丙型肝炎病毒感染:血清阳性率及其相关危险因素

Nepal Journal of Biotechnology Pub Date : 2019-01-15 DOI: 10.3126/NJB.V6I1.22335

G. Shah, K. Gurung, B. Baral, R. Gurung, B. Bhattachan

{"title":"Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors","authors":"G. Shah, K. Gurung, B. Baral, R. Gurung, B. Bhattachan","doi":"10.3126/NJB.V6I1.22335","DOIUrl":"https://doi.org/10.3126/NJB.V6I1.22335","url":null,"abstract":"Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) infections lead to chronic diseases and are the most common causes of liver cirrhosis and cancer in developing countries like Nepal. The study is carried out to determine the seroprevalence of HBV and HCV by using a Rapid kit method and Elisa Method to find out its risk factors. The cross-sectional study was done among blood donating people from 16th August 2016 to 19th November 2016. Blood donors in Pokhara Valley were screened for anti-HCV antibodies, anti-HBV antibodies using third generation ELISA kits and automated ELISA Processor in serology laboratory at Central Blood Transfusion Service (CBTS) of Nepal Red Cross Society (NRCS) in Pokhara, Nepal. 1777 (87.2%) units were male blood donors and 260 (12.6%) units were female donors out of 2037 participants. Gender wise, the ratio between male and female was 1:0.1. HBV and HCV infection rate in blood donors were detected at 0.7% (15/2037) and 0.5% (8/2037) respectively. HBV infection rate in volunteer blood donor people was 0.7% (14/1881) which was higher than the replacement donors i.e. 0.6% (1/156). Similarly, in HCV infection rate in volunteer donor were 0.4% (8/1881). HBV infected people are detected higher than the HCV infected people among the blood donors. In addition, there was no significant relationship between positive results of HBV and HCV tests with the gender, age, tattoo, donor type.","PeriodicalId":34186,"journal":{"name":"Nepal Journal of Biotechnology","volume":"277 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77550082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1