J. Mirčeta, M. Pelić, B. Božić, J. Petrović, M. Urošević, B. Stankov, D. Bugarski
{"title":"Prevalence of the giant liver fluke (fascioloides magna, bassi, 1875) in red deer (cervus elaphus) in the region of floodplain forests of northern Serbia","authors":"J. Mirčeta, M. Pelić, B. Božić, J. Petrović, M. Urošević, B. Stankov, D. Bugarski","doi":"10.46784/e-avm.v11i1.13","DOIUrl":"https://doi.org/10.46784/e-avm.v11i1.13","url":null,"abstract":"This is the first study offering insights into the prevalence of giant liver fluke in the population of red deer in the territory of Serbia. Giang liver fluke (Fascioloides magna, Bassi, 1875) is the most important liver parasite among wild ruminants in Europe, especially in the region of floodplain forests along the upper watercourse of Danube river. The main objective of this research was establishing the prevalence of giant liver fluke in the region of floodplain forests of northern Serbia. In the observed regions (hunting grounds), the population prevalence rates ranged from 0 to 80% with an average prevalence in positive herds being 70.6%. The total population of red deer, from the observed hunting grounds, exposed to the giant liver fluke includes 47.9% of red deer population in Serbia, which is 0.7% of the total hunting area of Serbia. Giant liver fluke is present in north-western regions of Serbia in a narrow area of floodplain forests along the watercourse of Danube and Sava rivers next to the border with Croatia. The red deers populating the wetland basin of “Gornje Podunavlje” migrate freely through the tri-border area of Hungary, Croatia and Serbia making a consistent epizootical unit. Moreover, the game migrates freely between Croatia and Serbia in the area of Posavina forests along the river Sava. All data obtained in this research are essential for further activities aimed at preventing the spread of this parasite within red deer population and thus decreasing consequent damages and losses.","PeriodicalId":34085,"journal":{"name":"Archives of Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42428432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Živkov Baloš, N. Popov, S. Vidaković, D. Ljubojević Pelić, M. Pelić, Ž. Mihaljev, S. Jakšić
{"title":"Electrical conductivity and acidity of honey","authors":"M. Živkov Baloš, N. Popov, S. Vidaković, D. Ljubojević Pelić, M. Pelić, Ž. Mihaljev, S. Jakšić","doi":"10.46784/e-avm.v11i1.20","DOIUrl":"https://doi.org/10.46784/e-avm.v11i1.20","url":null,"abstract":"In this paper, the electrical conductivity and concentration of free acids (acidity) of various types of honey collected from the area of Vojvodina was investigated. Physicochemical analyzes of parameters such as electrical conductivity and acidity of honey have an important role in defining the overall properties of honey and assessing the quality of honey. A total of 55 samples of honey were examined. Out of the total number of samples, 5 samples did not meet the requirements of the Republic of Serbia Regulations on quality of honey due to inadequate electrical conductivity. Free acid values measured in tested samples ranged from 1.5 to 30 mEq / kg, and the values of electrical conductivity were in the range between 0.08 and 1.99 mS/cm. Our assumption that there is a correlation between the tested parameters has been confirmed only in meadow and polyfloral honey. Further research on the physicochemical properties of Serbian honey is recommended and important in order to establish certification marks and criteria for assessing the quality of Serbian honey.","PeriodicalId":34085,"journal":{"name":"Archives of Veterinary Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46902089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. H. Cvjetković, V. Milošević, T. Petrović, D. Petrić, G. Kovačević, Jelena Radovanov, Aleksandra Patić, N. Nikolic, S. Mikić, Sofija Cvjetković, D. Cvjetković
{"title":"Diagnosis of west Nile neuroinvasive disease in humans","authors":"I. H. Cvjetković, V. Milošević, T. Petrović, D. Petrić, G. Kovačević, Jelena Radovanov, Aleksandra Patić, N. Nikolic, S. Mikić, Sofija Cvjetković, D. Cvjetković","doi":"10.46784/e-avm.v11i1.19","DOIUrl":"https://doi.org/10.46784/e-avm.v11i1.19","url":null,"abstract":"West Nile virus (WNV) is arbovirus distributed all around the world. In humans, 80% of infection cases are asymptomatic. In 20% of infected people, a febrile self-limiting illness is reported. WNV has the potential for fatal neuroinvasive disease. In 1% of cases, the infection may result in neuroinvasive disease with permanent neurological consequences or death outcome. Neurological forms may vary presenting with encephalitis, meningitis, meningoencephalitis or acute flaccid paralysis. Outbreaks with neurological forms of WNV infection were recorded in different areas of Greece, Italy, Romania, Hungary and Serbia. During the period from 2013 to 2016, 114 samples of cerebrospinal fluid and 107 serum samples were taken from 114 patients suspected of WNV neuroinvasive disease (WNND). The presence of specific anti-WNV IgM and IgG antibodies in cerebrospinal fluid (CSF) and sera samples were tested by WNV IgM and IgG ELISA (Euroimmun, Germany). In addition, 48 samples of CSF or/and serum of people with suspected WNV infection were examined by commercial molecular tests - real time RT-PCR (WNV Real-TM, Sacace biotechnologies, Italy). The IgM antibodies against WNV were present in 25.4% (29/114) of CSF samples, and in 31.8% (34/107) of serum samples tested from 114 patients suspected of WNND. The IgG antibodies against WNV were detected in 3.5% (4/114) of CSF samples, and in 11.2% (12/107) of serum samples. The WNV RNA was detected by real time RT-PCR test in 7 out of 48 (14.6%) CSF or/and serum samples. In this study, detection of IgM antibodies in CSF is more frequent than detection of WNV RNA in CSF or serum samples. WNV RNA detection in CSF is confirmatory diagnostic test but has limited utility in the diagnosis of WNV neuroinvasive disease due to low viremia level at the time of clinical presentation of the disease. The limitations in the use of ELISA IgM test are linked to cross - reactivity among flaviviruses and long persistence of IgM antibodies in the serum and CSF.","PeriodicalId":34085,"journal":{"name":"Archives of Veterinary Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49607215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Lazarov, G. Zhelev, Mihni Lytzkanov, K. Koev, V. Petrov
{"title":"Dynamics of microbial contamination in a poultry hatchery","authors":"I. Lazarov, G. Zhelev, Mihni Lytzkanov, K. Koev, V. Petrov","doi":"10.46784/e-avm.v11i1.15","DOIUrl":"https://doi.org/10.46784/e-avm.v11i1.15","url":null,"abstract":"The hatcheries may become contaminated with pathogenic bacteria which could spread in the hatchery through the air. That is why the implementation of an effective cleaning and disinfection program and the maintenance of good hygiene are extremely important for the normal course of production and for reducing the spread of infectious agents. In this study, data on the degree and dynamics of bacterial contamination in a poultry hatchery are presented. In the incubation sector, bacterial contamination on the surfaces was found to be low-level ranging from 0.25 to 4.43x101 CFU/ cm2 but in the air it was strongly influenced by the hatching. In the hatchery sector, bacterial contamination on the surface and in the air was high, with the highest values found on the egg shells (1.77x106 CFU/cm2), on the floor (3.2x104 CFU/cm2) and in the air (1.77x105 CFU/cm3) of hatcher cabinets during hatching. The results obtained show that the most important source of microbial contamination in the hatchery is the hatchery sector, especially during hatching, when highly contaminated materials as fluff, shells and dried secretions are released. In case of poor organization of working process, the bacteria could spread by air and contaminate the other sectors of the hatchery. The study confirms the importance of a different approach in the development of preventive measures, depending on the degree of risk in different zones in the hatchery, which is the basis for the effective management practice aimed at decreasing microbiological hazards in hatcheries.","PeriodicalId":34085,"journal":{"name":"Archives of Veterinary Medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46619933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}