Journal of Animal Reproduction and Biotechnology最新文献

{"title":"miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1","authors":"Haorong He, Dongmei Li, Yongtong Tian, Qinyao Wei, Felix Kwame Amevor, Congjiao Sun, Chunlin Yu, Chaowu Yang, Huarui Du, Xiaosong Jiang, Menggen Ma, Can Cui, Zhichao Zhang, Kai Tian, Yao Zhang, Qing Zhu, Huadong Yin","doi":"10.1186/s40104-022-00697-0","DOIUrl":"https://doi.org/10.1186/s40104-022-00697-0","url":null,"abstract":"BACKGROUND: The egg production performance of chickens is affected by many factors, including genetics, nutrition and environmental conditions. These factors all play a role in egg production by affecting the development of follicles. MicroRNAs (miRNAs) are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after transcription. In the animal reproduction process, miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells (GCs). RESULTS: In this study, we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy chickens. We identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken follicles. The function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p mimics. In the study, we used qPCR, western blot and flow cytometry to detect granulosa cell apoptosis, autophagy and steroid hormone synthesis. Confocal microscopy and transmission electron microscopy are used for the observation of autophagolysosomes. The levels of estradiol (E2), progesterone (P4), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by ELISA. The results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells, and also contributed in steroid hormones and reactive oxygen species (ROS) production. In addition, the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of miR-30a-5p. The rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory pathway. CONCLUSIONS: In summary, after deep miRNA sequencing on healthy and atretic follicles, the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"13 1","pages":"55-55"},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jasbsci.biomedcentral.com/track/pdf/10.1186/s40104-022-00697-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibiting nuclear factor erythroid 2 related factor 2-mediated autophagy in bovine mammary epithelial cells induces oxidative stress in response to exogenous fatty acids","authors":"Renxu Chang, Xudong Sun, Hongdou Jia, Qiushi Xu, Zhihao Dong, Yan Tang, Shengbin Luo, Qianming Jiang, Juan J. Loor, Chuang Xu","doi":"10.1186/s40104-022-00695-2","DOIUrl":"https://doi.org/10.1186/s40104-022-00695-2","url":null,"abstract":"BACKGROUND: In early lactation, bovine mammary epithelial cells undergo serious metabolic challenges and oxidative stress both of which could be alleviated by activation of autophagy. Nuclear factor erythroid 2 related factor 2 (NFE2L2), a master regulator of cellular redox homeostasis, plays an important role in the regulation of autophagy and oxidative stress. Thus, the objective of this study was to investigate the role of NFE2L2-mediated autophagy on oxidative stress of bovine mammary epithelial cells in response to exogenous free fatty acids (FFA). RESULTS: Exogenous FFA induced linear and quadratic decreases in activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD), and increases in the contents of reactive oxygen species (ROS) and malondialdehyde (MDA). Protein abundance of LC3-phosphatidylethanolamine conjugate (LC3-II) and the number of autophagosomes and autolysosomes decreased in a dose-dependent manner, while protein abundance of p62 increased in cells challenged with FFA. Activation of autophagy via pre-treatment with Rap attenuated the FFA-induced ROS accumulation. Importantly, FFA inhibited protein abundance of NFE2L2 and the translocation of NFE2L2 into the nucleus. Knockdown of NFE2L2 by siRNA decreased protein abundance of LC3-II, while it increased protein abundance of p62. Furthermore, sulforaphane (SFN) pre-treatment attenuated the FFA-induced oxidative stress by activating NFE2L2-mediated autophagy. CONCLUSIONS: The data suggested that NFE2L2-mediated autophagy is an important antioxidant mechanism in bovine mammary epithelial cells experiencing increased FFA loads.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"13 1","pages":"48-48"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jasbsci.biomedcentral.com/track/pdf/10.1186/s40104-022-00695-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of alpha-linolenic acid and essential amino acids on the proliferation and differentiation of C2C12 myoblasts","authors":"Dongjie Zhou, Xiao-Han Li, Song-Hee Lee, Geun Heo, X. Cui","doi":"10.12750/jarb.37.1.17","DOIUrl":"https://doi.org/10.12750/jarb.37.1.17","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81527035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro maturation on a soft agarose matrix enhances the developmental ability of pig oocytes derived from small antral follicles","authors":"Ji Eun Park, S. T. Lee, Geun-Shik Lee, Eunsong Lee","doi":"10.12750/jarb.37.1.34","DOIUrl":"https://doi.org/10.12750/jarb.37.1.34","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74010470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological remodeling in mouse vagina due to hormonal hypersecretion","authors":"Min-Gee Oh, Sang-Hwan Kim","doi":"10.12750/jarb.37.1.42","DOIUrl":"https://doi.org/10.12750/jarb.37.1.42","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83791764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved choleretic effect of Benachio-F®-based formula enriched with fennel extracts","authors":"Hye Jin Cho, Jun Su Im, Y. Kwon, K. Kang, Tae Min Kim","doi":"10.12750/jarb.37.1.27","DOIUrl":"https://doi.org/10.12750/jarb.37.1.27","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81546086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of rooster semen quality using CBB dye based staining method","authors":"S. Kim, Jae-Yeong Lee, Chan-Lan Kim, Yeong Gyu Ko, Bongki Kim","doi":"10.12750/jarb.37.1.55","DOIUrl":"https://doi.org/10.12750/jarb.37.1.55","url":null,"abstract":"The acrosome cap allows sperm to penetrate the egg membrane and produce male pronuclei within female chicken eggs, facilitating successful fertilization. Given this, it is important to establish practical methods for evaluating the integrity of the acrosome cap and thus the quality of the rooster’s sperm. There are several established methods for evaluating the acrosomes of mammalian sperm, but none of these methods are suitable for evaluating the acrosome status of rooster spermatozoa. Therefore, a simplified method for evaluating the rooster acrosome is needed. Here we evaluated the usefulness of CBB (coomassie brilliant blue) staining of the acrosome at concentrations of 0.04%, 0.08%, and 0.3% CBB solutions. Our data revealed a clear staining pattern for intact acrosome caps at 0.04% and 0.08% CBB but not at 0.3% CBB. This protocol revealed differences in acrosome integrity between fresh and frozen rooster sperm smears suggesting that CBB staining may facilitate easier semen evaluation in roosters. This protocol allows for the accurate differential staining of acrosome cap in","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82836336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The theranostic roles of extracellular vesicles in pregnancy disorders","authors":"I. Saadeldin, B. Tanga, S. Bang, Xun Fang, Ki-Young Yoon, Sanghoon Lee, Jongki Cho","doi":"10.12750/jarb.37.1.2","DOIUrl":"https://doi.org/10.12750/jarb.37.1.2","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86492946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of matrix metalloproteinases secreted by human hepatocarcinoma HepG2 cells","authors":"Young Jae Lee, Keun Cheon Kim, J. Lim, S. T. Lee","doi":"10.12750/jarb.37.1.62","DOIUrl":"https://doi.org/10.12750/jarb.37.1.62","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79385261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mental stress of animal researchers and suggestions for relief","authors":"Na Ahn, Jaehak Park, S. Roh","doi":"10.12750/jarb.37.1.13","DOIUrl":"https://doi.org/10.12750/jarb.37.1.13","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72701725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}