Jurnal Ilmu Dasar最新文献_第4页

Biological Aspects of Three Species Family Gobiidae in the Bilah River, Labuhanbatu Regency, Sumatera Utara Province 苏门答腊省拉布汉巴图县比拉河中虾蛄科三种的生物学特征

Jurnal Ilmu Dasar Pub Date : 2022-07-29 DOI: 10.19184/jid.v23i2.28160

K. Khairul, Ika Nazira Lubis

{"title":"Biological Aspects of Three Species Family Gobiidae in the Bilah River, Labuhanbatu Regency, Sumatera Utara Province","authors":"K. Khairul, Ika Nazira Lubis","doi":"10.19184/jid.v23i2.28160","DOIUrl":"https://doi.org/10.19184/jid.v23i2.28160","url":null,"abstract":"The Bilah river is known to have a fairly high fish biodiversity. Some species of Gobiidae are usually caught by fishermen in the estuary area. But until now there is no data and information related to the biological aspects of the Gobiidae family. The purpose of this study was to determine aspects of the size class and long relationship of the weight of Gobiidae fish caught by fishermen in the Bilah river. This study is exploratory, where fish sampling stations are determined based on fish habitat from fishermen's information (purposive sampling). Fish are caught using traditional fishing gear in the form of splint. The fish caught is then measured in total length and weight. Then the fish obtained based on the catch are grouped in small, medium, and large sizes. Data analysis using Microsoft Excel 2010 applications. In this study, three species of fish that are included in the family Gobiidae namely Butis butis, Butis aureus, and Eleotris fusca. The small category B. butis (9-11.9 cm) fish size class is obtained as many as 2 individuals, medium size (12-14.9 cm) 9 individuals, and large size (15-17.9 cm) 2 individuals. The small size class (6-8.9 cm) of B. aureus fish obtained 2 individuals, medium (9-11.9 cm) 8 individuals, and large 12-14.9 cm) 4 individuals. Small size class (9-11.9 cm) E. fusca as many as 6 individuals, medium (12-14.9 cm) 7 individuals, and large (15-17.9 cm) 3 individuals. The growth pattern of the crazy fish (B. butis) (value b = 0.10), B. aureus (value b = 0.27), and E. fusca (value b = 0.20).","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43712764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant of Effervescent Tablet Formulated from Combination of Secang Wood and Red Ginger Extracts 四苍木与红姜提取物联合配制泡腾片的抗氧化研究

Jurnal Ilmu Dasar Pub Date : 2022-07-28 DOI: 10.19184/jid.v23i2.28136

Titik Mardiyanti Sofyah, T. Tukiran, S. Sutoyo

{"title":"Antioxidant of Effervescent Tablet Formulated from Combination of Secang Wood and Red Ginger Extracts","authors":"Titik Mardiyanti Sofyah, T. Tukiran, S. Sutoyo","doi":"10.19184/jid.v23i2.28136","DOIUrl":"https://doi.org/10.19184/jid.v23i2.28136","url":null,"abstract":"Secang wood has strong antioxidant properties because it contains phenolic compounds such as flavonoids and brazilin. Also, red ginger has antioxidant derived from nonvolatile phenolic compounds. Until now, there has been no use of a combination of secang wood extract and red ginger specifically for the manufacture of effervescent tablets. The purpose of this study was to formulate an effervescent tablet by doing combination of secang wood and red ginger extracts with using various concentrations of citric and tartaric acids. The formulations conducted were three acid sources of 20%, 25%, 30%, using wet granulation. The evaluation subjected to the formulas included the physical properties of the granules, such as the water content and flow properties of the granules. Meanwhile, the evaluation performed to the tablet included the hardness test, tablet friability, weight uniformity, dissolution time, and acidity (pH). The analysis was carried out in accordance with the requirements of the Indonesian Pharmacopoeia Edition III and BPOM. The results obtained are effervescent tablet with an acid variation of 20% with physical quality values of water content of 2.91% and granule flow properties of 6.03 s. Meanwhile, the physical quality requirements of effervescent tablets were tablet hardness of 4.02 kp; tablet friability 0.46%; weight uniformity 0.132%; 71 s dissolution time; and 6.4 degrees of acidity.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43584168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Neural Network Performance on Pakcoy Leaf Fresh Weight Model 百叶鲜重模型的人工神经网络性能研究

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.27283

R. Rusdiana, L. I. Widuri, D. P. Restanto

{"title":"Artificial Neural Network Performance on Pakcoy Leaf Fresh Weight Model","authors":"R. Rusdiana, L. I. Widuri, D. P. Restanto","doi":"10.19184/jid.v23i2.27283","DOIUrl":"https://doi.org/10.19184/jid.v23i2.27283","url":null,"abstract":"The growth of leaf biomass can be predicted from an increase in the surface area and thickness of the leaves. Measurements of leaf biomass are approached with the fresh weight of the leaves. The relationship between biomass and leaf surface area commonly performed by regression analysis. The analysis requires assuming linear relationship between dependent variables and independent variables. Artificial Neural Network (ANN) is alternative that can be used to analyze the relationship of leaves and leaf biomass without requiring linear relationships. The research aimed to evaluate ANN performance in determining the fresh weight of pakcoy leaves based on leaf area parameters. Datasets in the study included leaf area datasets and length-width datasets. ANN architecture used Multi Layer Perceptron (MLP) with backpropagation. Ramsey’s test results showed that leaf area datasets is linier model and length-width datasets is nonlinier model. ANN performs well in predicting leaf fresh weight data on both nonlinear and linear models. The best ANN architecture for modeling the leaf fresh weight with leaf area is MLP (1-3-1) while the leaf fresh weight model with length and width is MLP (2-3-1).","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48638550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Pattern and Infill Percentage in 3D Printer for Phantom Radiation Applications 3D打印机中图案和填充率对幻影辐射应用的影响

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.27256

A. P. Hariyanto, Kurnia Hastu Christianti, A. Rubiyanto, N. Nasori, M. Haekal, E. Endarko

{"title":"The Effect of Pattern and Infill Percentage in 3D Printer for Phantom Radiation Applications","authors":"A. P. Hariyanto, Kurnia Hastu Christianti, A. Rubiyanto, N. Nasori, M. Haekal, E. Endarko","doi":"10.19184/jid.v23i2.27256","DOIUrl":"https://doi.org/10.19184/jid.v23i2.27256","url":null,"abstract":"3D printing technology was capable of fabricating phantoms to enhance quality assurance in radiation therapy. The ideal phantom has properties equivalent to the real tissue. However, 3D Printing has the limits to mimicking the attenuation properties of various tissues because during 3D printing there can be only one type of material. The purpose of this study was to evaluate the effect of infill percentage and infill patterns of 3D printing technology to simulate various types of tissue. This study used 25 samples measuring 5 × 5 × 1 cm3 from PETG material. The 20 samples were printed using variations infill percentages from 5 - 100% and the infill pattern in lines. The five samples were then printed with the infill percentage constant at 50% and used the infill pattern triangles, grid, gyroid, octet, and concentric. We used Computed Tomography (CT) to determine the Hounsfield Unit (HU) value for each sample and evaluated the suitability of each sample for phantom applications in radiation therapy and radiology. However, none of the samples was able to simulate compact bone. As a result, we found that PETG material could simulate the properties of soft tissue, fat, lung, kidney, liver, pancreas, and spongy bone. Thus, the study had shown promising potential for the fabrication of the anthropomorphic phantom of radiation therapy.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42718646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized Purification of CIDRα-PfEMP1 Plasmodium falciparum Recombinant Protein with Affinity Chromatography 亲和层析法纯化恶性疟原虫cdr α- pfemp1重组蛋白的优化

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.24127

Eqiel Navadz Akthar Alami, E. Sulistyaningsih, I. Kusuma, Sheilla Rachmania

{"title":"Optimized Purification of CIDRα-PfEMP1 Plasmodium falciparum Recombinant Protein with Affinity Chromatography","authors":"Eqiel Navadz Akthar Alami, E. Sulistyaningsih, I. Kusuma, Sheilla Rachmania","doi":"10.19184/jid.v23i2.24127","DOIUrl":"https://doi.org/10.19184/jid.v23i2.24127","url":null,"abstract":"Interaction of Cysteine-rich Interdomain Region (CIDR)α-Plasmodium falciparum Erythrocyte Membrane Protein (PfEMP1) and Endothelial Cell Receptors especially CD36 on host cells is main malaria pathogenesis, makes this domain as a malaria vaccine candidate. Recently, the development of the malaria vaccine is conducted by recombinant technology, and the purification of the CIDRα-PfEMP1 recombinant protein is a pivotal step. This study aimed to determine an optimal condition to purify the CIDRα-PfEMP1 recombinant protein by affinity chromatography through imidazole and NaCl concentration. The purified recombinant protein was visualized using SDS-PAGE and its concentration was measured using Image J software and Bradford Assay. The data were analyzed using SPSS 26 software, and the Paired T-Test analysis was conducted to compare the concentration of purified recombinant protein from two different methods. The result showed that thetarget band of purified recombinant protein was 27 kDa. The thickest target protein band was observed in purified recombinant protein using 140 mM imidazole and 300 mM NaCl. The recombinant protein concentration using Image J software was 0.025 µg/µL, while the Bradford Assay was 0.56 µg/µL. The Paired T-Test analysis has a significance value of 0.010 (p<0.05), meaning there was a significant difference between the concentration measurement using Image J software and Bradford Assay. In conclusion, the optimized condition to purify the CIDRα-PfEMP1 recombinant protein by affinity chromatography was using 140 mM imidazole and 300 mM NaCl. It is suggested to measure the purified CIDRα-PfEMP1 recombinant protein concentration using the Bradford Assay method due to its convenience and sensitivity.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49093279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flooded Area Mapping and Its Relationship to the Land Use, Soil Type, and Rainfall in North Konawe Regency 北科纳威县洪泛区制图及其与土地利用、土壤类型和降雨的关系

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.23898

B. E. Cahyono, Ervina Ikke Septiyas Putri, A. T. Nugroho

{"title":"Flooded Area Mapping and Its Relationship to the Land Use, Soil Type, and Rainfall in North Konawe Regency","authors":"B. E. Cahyono, Ervina Ikke Septiyas Putri, A. T. Nugroho","doi":"10.19184/jid.v23i2.23898","DOIUrl":"https://doi.org/10.19184/jid.v23i2.23898","url":null,"abstract":"The flood incident in North Konawe Regency, Southeast Sulawesi that occurred on June 2nd, 2019 was the largest flood disaster in that area since the last 42 years, so it is interesting to study. As part of disaster risk management, it is necessary to do flood mapping to determine the distribution of flooded areas and identify areas that have potential for flooding. Mapping of flood inundation areas was carried out using Sentinel-1 data. Land use, rainfall and soil types are used as an analysis of their were relationship to the distribution of flood. The distribution of flood based on the identification of the presence of inundation covered 3 sub-districts, namely Oheo District, Asera District and Andowia District. Correlation of flood distribution to the land use, rainfall and soil type was identified using Pearson correlation value (r). The correlation between flood distribution and land use was -0.59 that indicates the correlation is moderate. Moreover, the correlation of flood distribution to the rainfall was 0 which means the correlation was very weak, and lastly, the correlation value of the flood distribution with soil type was 0.88 or the correlation was very strong.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42667252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The 32 kDa Outer Membrane Proteins of Klebsiella pneumoniae Acts as A Bacterial Adhesin 肺炎克雷伯菌32 kDa外膜蛋白作为细菌粘附素

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.25435

D. Agustina, M. A. Shodikin, Bima Setia Sandya Nugraha, D. Mufida, E. Suswati, Bagus Hermansyah

{"title":"The 32 kDa Outer Membrane Proteins of Klebsiella pneumoniae Acts as A Bacterial Adhesin","authors":"D. Agustina, M. A. Shodikin, Bima Setia Sandya Nugraha, D. Mufida, E. Suswati, Bagus Hermansyah","doi":"10.19184/jid.v23i2.25435","DOIUrl":"https://doi.org/10.19184/jid.v23i2.25435","url":null,"abstract":"Klebsiella pneumoniae is a bacterium that often causes infection in the human body. At present K. pneumoniae can resist some of the antibiotics it has associated with modification of one of the virulence factors possessed by K. pneumoniae. One virulence factor of K. pneumoniae as pathogen bacteria is Outer Membrane Protein (OMP). The study of adhesin factors in K. pneumoniae involving hemagglutinin and adhesin proteins that have been found in the OMP 20 kDa and 40 kDa, but there is still no research that discusses the role of 32 kDa OMP as a hemagglutinin protein and adhesin. The purpose of this study is to determine the role of 32 kDa outer membrane of K. pneumoniae as hemagglutinin and adhesin proteins. After isolation of Outer Member Protein (OMP) from the K. pneumoniae, which then carried out a hemagglutination test using mice erythrocyte cells and adhesion test using mice enterocyte cells. The results of the hemagglutination test using mice erythrocyte cells obtained the highest hemagglutination titer for the molecular weight of 32 kDa in titers 1/4. The adhesion index with dilution titer has a significant relationship, with a conversion coefficient of 0,813 which means the dilution titer with the OMP adhesion index has a strong relationship with the direction of a positive relationship. The regression test results obtained an R-value of 0,813 which shows a strong relationship, while the R2 value is 66.1%. Conclusion in this study is 32 kDa outer membrane proteins of K. pneumoniae acts as a bacterial adhesin.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42388230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Profile of Reproductive Hormones of Nypa Palm Worm Namalycastis rhodochorde (Polychaeta: Neriedidae) 棕榈线虫(Namalycastis rhodochorde)生殖激素谱(多毛目:蚕科)

Jurnal Ilmu Dasar Pub Date : 2022-07-27 DOI: 10.19184/jid.v23i2.24095

Junardi Junardi, T. Setyawati, A. H. Yanti

引用次数: 0

Vibration Test Fixtures Design for Payload of Tubular Telemetry System 管式遥测系统载荷振动测试夹具设计

Jurnal Ilmu Dasar Pub Date : 2022-01-17 DOI: 10.19184/jid.v23i1.23894

M. Muksin, M. G. P. P. Pratama, Y. Nugroho, Yusuf Giri Wijaya, N. Ula, A. Wirawan

{"title":"Vibration Test Fixtures Design for Payload of Tubular Telemetry System","authors":"M. Muksin, M. G. P. P. Pratama, Y. Nugroho, Yusuf Giri Wijaya, N. Ula, A. Wirawan","doi":"10.19184/jid.v23i1.23894","DOIUrl":"https://doi.org/10.19184/jid.v23i1.23894","url":null,"abstract":"Vibration testing on telemetry system payload is needed to determine its resistance to vibration during operation. Vibration testing of telemetry system payloads requires a test fixture. Test fixture is used to transmit vibration from shaker to telemetry system payload. The payload of telemetry system to be tested is a tubular. The test fixture in this study was designed using SolidWorks software. The material used for manufacture of test fixture is aluminum. Aluminum material was chosen because it has good rigidity with a relatively light mass. Stiffness and mass properties affect to natural frequency value of test fixture, which must be greater than the frequency range of operating telemetry system. Data collection uses two accelerometers placed on test fixture. Sine sweep vibrations are given from 5 Hz to 2000 Hz with a constant amplitude of 1 g and a sweep rate of 1 octave/minute. The test produces three peaks that they are candidates as natural frequencies, i.e at a frequency of 1532 Hz with an amplitude of 2.898 g, a frequency of 1706 Hz with an amplitude of 6.582 g, and at a frequency of 1806 Hz with an amplitude of 6.472 g. From those three natural frequencies, the second natural frequency at 1706 Hz is the most critically because it has the largest response value.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41556854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Black Soybean (Glycine soja) Extract on Ovarian Histology of Swiss Webster Mice (Mus musculus L) Unilateral Ovariectomy 黑大豆提取物对瑞士韦氏小鼠（Mus musculus L）单侧卵巢切除后卵巢组织学的影响

Jurnal Ilmu Dasar Pub Date : 2022-01-14 DOI: 10.19184/jid.v23i1.10787

M. Mahriani, E. Utami, Nindita Fitria Primasari

{"title":"Effect of Black Soybean (Glycine soja) Extract on Ovarian Histology of Swiss Webster Mice (Mus musculus L) Unilateral Ovariectomy","authors":"M. Mahriani, E. Utami, Nindita Fitria Primasari","doi":"10.19184/jid.v23i1.10787","DOIUrl":"https://doi.org/10.19184/jid.v23i1.10787","url":null,"abstract":"Estrogen production can be reduced due to menopause and ovariectomy. Decreased estrogen levels in the body cause physiological changes in the female reproductive system. Therefore, the intake of estrogen from outside the body is needed, in this case phytoestrogens from soy bean extract can be used to replace the role of endogenous estrogen. The study used 30 Swiss Webster mice (Mus musculus) aged 90 days weighing 35 grams which were unilateral ovariectomy. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice are ready to be used as test animals after an estrogen deficiency period of 60 days. Mice were divided into five groups: negative control, positive control, dose 0.21, 0.42, and 0.63 g/mL/day . Data obtained from the results of the study were analyzed using the One Way ANOVA test with a confidence level of 99% or α = 0.01. Based on the results of the study, the administration of black soybean flour extract in unilateral ovariectomy mice can increase the average number of primordial follicles at doses of 0.42 g/ml/day and dosage 0.63 g/ml/day, primary follicles at a dose of 0.42 g/ml/day and doses of 0.63 g/ml/day, and secondary follicles at a dose of 0.63 g / ml / day, and tend to increase the average number of primordial follicles at doses of 0.21 g/ml/day, follicles primary at doses of 0.21 g/ml/day, secondary follicles at doses of 0.21 g/ml/day and 0.42 g/ml/day, de Graaf follicles at doses of 0.21, 0. 42 and 0.63 g/ml/day.","PeriodicalId":33444,"journal":{"name":"Jurnal Ilmu Dasar","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46997924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0