Agrarnaia nauka EvroSeveroVostoka最新文献

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Variability and interrelation of breeding-valuable traits of varieties and lines of spring soft wheat in the conditions of the Middle Volga region 中伏尔加河地区春软小麦品种系育种价值性状的变异及相互关系
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.739-748
I. F. Demina
{"title":"Variability and interrelation of breeding-valuable traits of varieties and lines of spring soft wheat in the conditions of the Middle Volga region","authors":"I. F. Demina","doi":"10.30766/2072-9081.2023.24.5.739-748","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.739-748","url":null,"abstract":"The article presents the results of a three-year study (2020-2022) of 16 varieties and 17 breeding lines of spring soft wheat bred by the Federal Scientific Center for Bast Fiber Crops in the soil and climatic conditions of the Penza region. The crop productivity in the experiment varied between 1.77-4.15 t/ha. Seven samples demonstrated the highest productivity: Nastavnik, Eritrospermum 70/04-3, Irvita, Sensei, Eritrospermum 20/08-7, Lutestsens 1/12-19, Eritrospermum 15/08-4, which exceeded the standard variety Arkhat by 0.27-0.71 t/ha. During the studies the yield was related to the average variable trait (CV =11.9-19.5 %). The variation of the duration of the growing season of the studied spring wheat samples averaged 81-93 days. It was shorter in 2021 (from 67 to 80 days), the longest in 2022 (from 91 to 100 days). The height of spring soft wheat plants in the studies was a low-variable feature (CV = 3.4-8.5 %) and averaged 82.1-100.2 cm over the years. The ear grain content varied from 18 pieces in the Erythrospermum 25/08-11-18 in 2022 up to 40 pieces in the Nastavnik variety in 2021. The trait was medium-variable (CV = 11.3-18.5 %). The highest average productivity of an ear was noted in 2022 – 1.08 g, and the lowest in 2020 – 0.92 g. The high mass of grain per ear was noted in Nastavnik variety – 1.40 g, Eritrospermum 70/04-3 – 1,38 g, Irvita – 1.35 g, which were characterized by high yield. Eritrospermum 70/04-3, Eritrospermum 20/08-7 and Irvita selected by the genotype productivity were characterized by bulky grains of 40.6-43.2 g. A significant correlation was observed between the yield and the grain weight per plant (r = 0.908...0.924, P≤0.001). The studied patterns can be used in ecological breeding of soft spring wheat.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal patterns of changes in antioxidant and microelement parameters of milk from black-and-white cows 黑白奶牛牛奶中抗氧化剂和微量元素参数的季节性变化模式
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.858-867
A. A. Savina, D. A. Voronina, S. Yu. Zaitsev
{"title":"Seasonal patterns of changes in antioxidant and microelement parameters of milk from black-and-white cows","authors":"A. A. Savina, D. A. Voronina, S. Yu. Zaitsev","doi":"10.30766/2072-9081.2023.24.5.858-867","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.858-867","url":null,"abstract":"Studying the antioxidant status of cow's milk can provide information both on the physiological state of the productive animal and on the resulting product in terms of its functional value, taste and characteristics affecting storage. The purpose of the work is to study milk samples from Holstein cows of the black-and-white breed with different periods of lactation (up to 30-60 days, 60-90, 90-180, more than 180 days) for the activity of antioxidant protection factors during a number of months of autumn, winter and spring periods. The total amount of water-soluble antioxidants (TAWSA) of milk was determined by the amperometric method, the activity of ceruloplasmin by the modified Ravin method, the microelement composition by the method of atomic adsorption spectrometry, the concentration of vitamin C by the titrimetric method, and the catalase number by the permanganatometry method. Patterns have been identified between the seasonal dynamics of zinc and catalase in milk: in January, in a group of animals with a lactation period of 30-300 days, catalase activity and zinc content are higher by 21.6 and 17.4 % , respectively, than in cows with a lactation period of 15-30 days; in February, on the contrary, these indicators are higher in the group of animals with a lactation period of 15-30 days (by 29.6 and 30.4 %, respectively); in March, in the group of animals with a lactation period of 15-30 days, catalase activity and zinc levels are lower (by 26.3 and 10.6 %) than in cows with a lactation period of more than 30 days. There is a decrease in TAWSA with an increase in the number of lactation days. The concentration of TCWA in the milk of cows lactating for more than 180 days decreased in November and March, by 25.9 and 13.5 %, respectively, compared with a lactation period of 30-60 days, in February – by 18.8 % compared with animals lactating 60-90 days. The obtained data on TAWSA are directly proportional to changes in vitamin C and inversely proportional to ceruloplasmin. The data obtained can serve as important guidelines in determining “reference indicators” for cows’ milk.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"155 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Normalization of protein and carbohydrate nutrition for high productive cows during a dry period 高产奶牛在干旱期蛋白质和碳水化合物营养的正常化
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.820-829
L. N. Kuzmina, A. P. Kartashova, S. S. Kuzmin
{"title":"Normalization of protein and carbohydrate nutrition for high productive cows during a dry period","authors":"L. N. Kuzmina, A. P. Kartashova, S. S. Kuzmin","doi":"10.30766/2072-9081.2023.24.5.820-829","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.820-829","url":null,"abstract":"The article provides the results of study of protein and carbohydrate nutrition for high productive cows with milk yield of 10 thousand kg during dry period. There has been carried out the research for determining the feed protein disintegration and effectiveness of its use in cow rations. The carbohydrate nutrition for Holstein-Kholmogory cows during dry period taking into account quantity and quality of neutral detergent fiber (NDF) and acid detergent fiber (ADF) was studied. The science-economic experiment was conducted in the “Polyarnaya Zvezda”, Murmansk region in 2015 and 2021. The disintegration of feed crude protein (DP) was determined by the in sacco method, by incubation of average samples of single feeds in nylon bags in the rumen during 12 hours. Content of crude protein in feed samples before and after incubation was determined by the content of total nitrogen by the Kjeldahl method. Digestibility in the intestine of feed protein not disintegrated in rumen was determined by the mobile synthetic bag method. NDF and ADF were determined by the method of Van Soest and Southgate modified by N. N. Semina. In the experiment of protein nutrition, the content of crude protein in the ration of control and experimental groups of cows was 16 %. The content of disintegrated protein in the control group was 59.0 %, in the experimental group – 67.0 %. In the experiment of carbohydrate nutrition, in the control group crude fiber was 19.4 %, neutral detergent fiber – 38.9 %, acid detergent fiber – 23.0 % of dry matter. In the ration of experimental group of cows, the content of crude fiber was 22.4 %, neutral detergent fiber – 47.2 %, acid detergent fiber – 27.6 %. Animals of the experimental group whose ration had a crude protein disintegration of 67.0% used nitrogen more efficiently, utilized a nutritious of ration better. During the research it has been established, that the content of neutral detergent fiber of up to 47.2 % and acid detergent fiber of up to 27.6 % results in increase in digestibility of ration nutritious, improves nitrogen metabolism, intensify microbiological activity in cow rumen.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mycotoxins of phytopathogenic fungi and mycotoxicosis: a historical essay (review) 植物病原真菌的真菌毒素与真菌中毒:一篇历史论文(综述)
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.703-719
I. V. Artamonov
{"title":"Mycotoxins of phytopathogenic fungi and mycotoxicosis: a historical essay (review)","authors":"I. V. Artamonov","doi":"10.30766/2072-9081.2023.24.5.703-719","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.703-719","url":null,"abstract":"Mold fungi and the mycotoxins they produce have invariably accompanied humans, since the basis of its existence was agriculture, which allows to get more products than was necessary for consumption, as well as requiring the storage of crops between seasons. This provided a favorable ecological niche for molds, and stored agricultural products became the main source of mycotoxins in the diets of humans and farm animals. Since then, mycotoxins have been the cause of many large-scale poisonings and have caused significant damage to agriculture. The article provides a brief historical sketch and the most significant stages of the study of mycotoxins, which are the cause of frequent and large-scale lesions, reveals the specific circumstances that caused the researchers' keen interest in this group of substances. For a long time, the immediate cause of poisoning caused by mycotoxins remained unknown due to the weak technical equipment of researchers and the isolation of active substances was impossible, which, however, was not an obstacle to the development of methods to combat infection with mold fungi and poisoning caused by mycotoxins. The improvement of the technical base of research in the middle of the 20th century led to an increase in the number of discovered mycotoxins and the discovery of new toxic effects of already known compounds. Mycotoxins remain one of the most dangerous contaminants in both farm animal feed and food, despite the fact that in many countries they are subject to strict regulation and control. In the context of the increasing intensification of agriculture, a decrease in the species diversity of agricultural crops, the problem of the spread of mold fungi and, as a result, the degree of mycotoxin damage to agricultural products does not lose its importance, and the study of the history of the study of mycotoxins and their producers can provide important information on the spread of mold fungi in new economic conditions.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"1 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of sunflower rust pathogen biotypes in the regions of the Russian Federation 俄罗斯联邦地区向日葵锈病病原菌生物型的多样性
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.792-798
N. M. Araslanova, T. S. Antonova, S. L. Saukova, M. V. Iwebor
{"title":"Diversity of sunflower rust pathogen biotypes in the regions of the Russian Federation","authors":"N. M. Araslanova, T. S. Antonova, S. L. Saukova, M. V. Iwebor","doi":"10.30766/2072-9081.2023.24.5.792-798","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.792-798","url":null,"abstract":"The spread and development of sunflower rust in the regions of the Russian Federation is due to the emergence of new biotypes of the pathogen, which have overcome the resistance of cultivated varieties and hybrids. The aim of the research was to identify isolates of the sunflower rust pathogen collected in 2020-2022 in 8 regions of crop cultivation according to the internationally accepted classification: Krasnodar, Volgograd, Saratov, Voronezh, Ulyanovsk, Penza, Orenburg, and Tambov regions of the Russian Federation. Differential lines of sunflower rust resistance (CM 90, CM 29, R-386, HAR-1, HAR-2, HAR-3, HAR-4, HAR-5) and the variety VNIIMK 8833 susceptible to all races of the pathogen were used for testing. A total of 280 isolates of urediniospores of the parasite collected from infected plants of different sunflower genotypes were identified according to the internationally accepted classification. In this sample, 27 biotypes of rust pathogens were differentiated. There have been identified fifteen biotypes not previously found in Russia, their code numbers are 320, 701, 703, 710, 720, 721, 730, 742, 744, 747, 761, 765, 766, 771 and 777. The most virulent biotype 777 was detected in 2022 among isolates from the Volgograd region. The biotype with the code number 700 was detected in all the regions presented, accounting for 50 % of the sample of isolates studied. It is advisable to carry out sunflower breeding for its resistance.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"39 10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135936042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of agronomically valuable synergistic effects in binary cultures of soil streptomycetes 土壤链菌二元培养协同增效效应的研究
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.799-809
N. A. Bokov, R. I. Abubakirova, I. G. Shirokikh
{"title":"Study of agronomically valuable synergistic effects in binary cultures of soil streptomycetes","authors":"N. A. Bokov, R. I. Abubakirova, I. G. Shirokikh","doi":"10.30766/2072-9081.2023.24.5.799-809","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.799-809","url":null,"abstract":"Co-culturing of microorganisms can be an effective way to control their enzymatic activity and synthesis of secondary metabolites based on the phenomenon of synergism. The effect of co-culturing in different combinations of four local Streptomyces isolates on their cellulase activity, antiphytopathogenic and phytoregulatory effects was evaluated. Strains S. antimycoticus 8Al3 and Streptomyces sp. H 27-25 exhibited an antagonistic effect against fungi of the genus Fusarium and Alternaria. Binary cultures were composed so that the cellulolytic and antagonist strains were present. Significantly higher cellulase activity in comparison with monoculture (3800 units/10 min/g) was demonstrated by the binary association of strains S. griseoaurantiacus Mb 4-2 + S. antimycoticus 8Al3 (13215 units/10 min/g). When these isolates were co-cultured, an increase in the antifungal activity of the binary culture (18.76±6.1 mm) compared to that of its constituent S. antimycoticus 8Al3 (11.09±6.39 mm) was also noted. The mean value of the growth inhibition zones of phytopathogenic fungi (18.76±6.1 mm) was comparable with that of the reference preparation, the commercial antimycotic terbinafine (19.8±6.2 mm). Binary artificial associations in tests for phytoregulatory action, as well as monocultures of their constituent streptomycete isolates, had no significant effect on germination and morphometry of wheat seedlings. The absence of phytoinhibitory effect of the binary culture S. griseoaurantiacus Mb 4-2 + S. antimycoticus 8Al3, combined with agronomically valuable properties such as cellulase activity and antagonism to phytopathogenic fungi give grounds for its use for the development of a soil-improving biopreparation. Further study of the properties of the binary association, in particular, the possibility of its combination with PGPR-bacteria (Plant Growth-Promoting Rhizobacteria), will constitute the subject of further research.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method of ISSR-marking in the study of genetic diversity of flax varieties 亚麻品种遗传多样性研究中的issr标记方法
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.767-776
T. A. Bazanov, I. V. Ushapovsky, N. N. Loginova, E. V. Smirnova, P. D. Mikhaylova
{"title":"Method of ISSR-marking in the study of genetic diversity of flax varieties","authors":"T. A. Bazanov, I. V. Ushapovsky, N. N. Loginova, E. V. Smirnova, P. D. Mikhaylova","doi":"10.30766/2072-9081.2023.24.5.767-776","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.767-776","url":null,"abstract":"The genetic diversity of crop varieties should be assessed using modern molecular markers. One of the most promising and simple methods of plant genetic analysis is intermicrosatellite labeling (ISSR). The purpose of this research is to study the polymorphism of varieties of fiber flax and linseed flax included in the State Register of Breeding Achievements of the Russian Federation, as well as local forms of fiber flax using ISSR markers. The objects for the research were 83 samples from the collection of the Federal Research Center for Bast Fiber Crops, among them 24 varieties of linseed flax, 56 varieties of fiber flax of domestic and foreign breeding, and three local forms from the Central Non-Black Earth Region. Genetic analysis was carried out by PCR method using a set of 25 ISSR primers. Detection of amplification products was carried out using electrophoresis in 1.5% agarose gel. Based on intraspecific selection of flax varieties to oilseed and fiber forms, a cluster analysis was performed according to the results of ISSR labeling independently in each of two groups that allowed to increase the reliability of conclusions on the existing dependences. Dendrograms of the genetic similarity of the studied samples of linseed flax and fiber flax were studied independently from each other. As a result of the analysis, linseed flax varieties were divided into five clusters, which made it possible to establish dependencies related to their origin (originator of the variety) and oil content in the seed. The dendrogram which included varieties of fiber flax and its local forms, revealed twelve subclusters forming three uneven groups. Almost every group had its own characteristic feature, not related to the geography of origin. The grouping of varieties occurred mainly on the basis of their various characteristics in terms of flax fiber content, ripening time and resistance to environmental stress factors. The results of the research show the applicability of using the ISSR-markers as a valid and accessible method for developing the elements of marker-associative breeding of bast fiber crops.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Search for genetic markers for selection and breeding aimed at increasing birth weight of piglets 为提高仔猪出生体重的选择和育种寻找遗传标记
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.839-848
E. A. Romanets, T. S. Romanets, O. L. Tretyakova, L. V. Getmantseva
{"title":"Search for genetic markers for selection and breeding aimed at increasing birth weight of piglets","authors":"E. A. Romanets, T. S. Romanets, O. L. Tretyakova, L. V. Getmantseva","doi":"10.30766/2072-9081.2023.24.5.839-848","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.839-848","url":null,"abstract":"With the intensive increase in the fertility traits of sows, the problem of decreasing the weight of piglets at birth has arisen. In this connection, the search for genetic variants associated with the birth weight of piglets is of particular relevance and scientific significance. The aim of the work was to identify genetic variants associated with piglet weight at birth and test them to select optimal genetic markers for selection and breeding work to improve reproductive performance of pigs on the basis of full genomic genotyping data using the Fst method. The studies were conducted in 2020-2022 on Large White pigs (n=239) bred at CJSC Plemzavod-Yubileiny in the Tyumen Region. Genotyping was performed using GeneSeek® GGP Porcine HD Genomic Profiler v1 (Illumina Inc., USA). Genomic data were filtered according to the following parameters --geno 0.1, -mind 0.1, -maf 0.05, -hwe 1e-7, --indep-pairwise 50 5 0.8. To identify genomic regions associated with piglet birth weight, there was used Fst statistics comparing genetic variants in pigs between two groups with high and low indices. Those in which the Fst values exceeded the quantile level of 0.999 were considered significant variants. Student's test was used to evaluate the significance of the effects of variant genotypes on the birth weight and number of piglets at birth. The results showed that there was a moderate negative relationship (-0.351) between piglet birth weight and number of piglets at birth. 17 SNPs associated with birth weight of piglets were identified, 9 of which were located in the KIF13A, STK24, FDFT1, ADGRD1, STX2, TMEM132D, ENSSSCG00000054866, ENSSSCG00000058459 genes, as well as SNPs rs81450496, rs80887103 in intergenic regions have been identified as promising genetic markers for increase in birth weight of piglets. The results obtained can be used to create domestic breeding technologies that improve the efficiency of pig breeding.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Loci of the genotyping panel by sequencing using AgriSeq technology in the Manych Merino breed 利用AgriSeq技术对曼尼奇美利奴品种的基因分型面板进行测序
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.849-857
A. Yu. Krivoruchko, A. A. Likhovid, A. A. Kanibolotskaya, T. Yu. Saprikina, M. Yu. Kuharuk, O. A. Yatsyk
{"title":"Loci of the genotyping panel by sequencing using AgriSeq technology in the Manych Merino breed","authors":"A. Yu. Krivoruchko, A. A. Likhovid, A. A. Kanibolotskaya, T. Yu. Saprikina, M. Yu. Kuharuk, O. A. Yatsyk","doi":"10.30766/2072-9081.2023.24.5.849-857","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.849-857","url":null,"abstract":"Using the genotyping of sheep of the Manych Merino breed on the basis of Illumina BeadChip Ovine 600K, loci suitable for genotyping by sequencing animals of this breed were found. Single nucleotide polymorphisms with a high frequency of occurrence in the range of 0.2850-0.3149 homozygotes of both wild and mutant variants were identified. Heterozygous variants of these substitutions occurred with a frequency of 0.379±0.012. The number of polymorphisms corresponding to the selected criteria was 521. Analysis of the location of the detected SNPs in the sheep genome showed their presence along the entire length of the genotyped DNA region. The largest number of polymorphisms were found on chromosomes 1, 2, 3, 17 and X. The least polymorphisms were detected on chromosomes 18, 21, 24 and 25. The resulting set of substitutions will effectively solve the problems of confirming the authenticity of the origin of sheep of the Manych Merino breed, accurately identify animals in the process of breeding work, and account for inbreeding in the population. The proposed set of SNPs is recommended both for use in genotyping by sequencing of a new generation, and for customization of SNP biochips.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"829 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparing the productivity of potato plants when growing mini-tubers in the conditions of air hydroponics and in the pots with soil substrate 比较空气水培和有基质盆栽条件下马铃薯微型块茎的产量
Agrarnaia nauka EvroSeveroVostoka Pub Date : 2023-10-31 DOI: 10.30766/2072-9081.2023.24.5.757-766
O. S. Khutinaev
{"title":"Comparing the productivity of potato plants when growing mini-tubers in the conditions of air hydroponics and in the pots with soil substrate","authors":"O. S. Khutinaev","doi":"10.30766/2072-9081.2023.24.5.757-766","DOIUrl":"https://doi.org/10.30766/2072-9081.2023.24.5.757-766","url":null,"abstract":"The article provides the results of comparative tests on growing mini-tubers of potato in greenhouse culture using the pots with soil substrate and in the conditions of air hydroponics without hard substrates by treatment of root system with water-air nutrient mixture. The results of field testing of seed qualities of mini-tubers obtained by various methods are given. The experiments were carried out in Moscow region in potato varieties Fioletovy and Severnoe siyanie. On the basis of the research, it has been established that using the method of obtaining mini-tubers in the conditions of air hydroponics it is possible to increase the seed reproduction coefficient raising the quantitative characteristics of the yield. When growing in the conditions of air hydroponics in the Severnoe siyanie variety there was recorded two time increase of reproduction coefficient and obtained 19.2 mini-tubers per plant. At the same time, in greenhouse culture the average yield was 9.2 mini-tubers per plant. In Fioletovy variety, the reproduction coefficient in air hydroponic plants was 1.8 times higher than in greenhouse culture, 29.1 and 16.1 mini-tubers per plant, respectively. In the yield of greenhouse mini-tubers there were no seeds of small fractions, while in air hydroponic parts there were 25 % of them in Severnoe siyanie variety and 19 % in Fioletovy one. The number of fractions of large seeds more than 30 mm in size was much higher in greenhouse parts than in air hydroponic ones, 49.9 % against 1.82 % in Severnoe siyanie variety and 38.5 % against 1.3 % in Fioletovy variety. By comparative field tests of plants obtained from seed greenhouse and air hydroponic mini-tubers there has not been established significant differences in their yield and reproduction coefficient.","PeriodicalId":33301,"journal":{"name":"Agrarnaia nauka EvroSeveroVostoka","volume":"3 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135935328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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