BioedusciencePub Date : 2023-08-24DOI: 10.22236/jbes/11699
Rizky Nurhayati, Suparti
{"title":"The Effect of Banana Stem Powder on the Productivity of the Brown Oyster Mushroom (Pleurotus cystidiosus)","authors":"Rizky Nurhayati, Suparti","doi":"10.22236/jbes/11699","DOIUrl":"https://doi.org/10.22236/jbes/11699","url":null,"abstract":"Background: Brown Oyster Mushroom (Pleurotus cystidiosus) is a type of mushroom that has different advantages from other oyster mushrooms. Generally, farmers make planting media for brown oyster mushrooms made from sawdust of sengon wood. The availability of raw materials for sawdust of sengon wood is increasingly difficult to obtain. So we need other alternative materials as a source of nutrition, one of which is banana stem waste. This study aims to determine the effect of banana stem flour on the diameter of the cap, the number of fruiting bodies, and the wet weight of the fungus. Methods: This study used a completely randomized design (CRD) which consisted of one factor. This study used 4 treatments and 3 replications. Data was tested by quantitative descriptive analysis using the Analysis of Variance (ANOVA) test. Results: Banana stem flour has a significant effect on all parameters. The JM2 treatment resulted in a cap diameter of 10.3 cm, mushroom fruiting bodies of 10.6 and wet mushroom weight of 103.8 g. Conclusion: The addition of banana stem powder can increase productivity in the parameters of mushroom cap diameter, number of mushroom fruiting bodies, and wet weight of mushrooms, which can be used by readers or researchers. Utilization of waste such as sawdust of sengon wood and waste of banana fronds for the cultivation of brown oyster mushrooms is one way of handling plantation waste.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44721559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-08-24DOI: 10.22236/jbes/12275
Jahanzaib Ali
{"title":"Novel CRISPR-Cas9 approach to combat with COVID-19","authors":"Jahanzaib Ali","doi":"10.22236/jbes/12275","DOIUrl":"https://doi.org/10.22236/jbes/12275","url":null,"abstract":"Background: The recent outbreak of Corona Virus has affected people all over the world so that WHO declared it a pandemic. This pandemic has become a major problem for people, health professionals, and research institutes that how to combat SARS-CoV-2. The objective of this review article is to provide a brief study that CRISPR/Cas9 can be used to combat SARS-CoV-2. Methods: The comparison of three genome editing techniques (ZNF, TALEN, and CRISPR/Cas9) is done. The mechanism of action of CRISPR/Cas9 is discussed. Results: The studies provided in this review article suggest that it is difficult to recognize this virus as they have their own metabolic machinery and they replicate their selves in the host cell and consume host cellular products to perform their own functions. The COVID-19 proteins may contain some specific areas that are suitable targets for therapy, such as tiny inhibitor molecules for viral polymerase, or impede the attachment of viruses to the receptor sites, for example, viral coat proteins can be used for vaccination purposes. Conclusion: CRISPR-Cas9 can be used to control the SARS-CoV-2 genome from replication and spread to other parts of the body as it can edit the genome quite efficiently. This proposed model will help in targeting the SARS-CoV-2 genome more precisely in the future.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47234075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-08-24DOI: 10.22236/jbes/9749
Ajenk Ayunda, R. Yuniati, W. Handayani
{"title":"Comparative Study of The Physiological Condition of Six Tree Species to Air Pollution in Depok City, West Java","authors":"Ajenk Ayunda, R. Yuniati, W. Handayani","doi":"10.22236/jbes/9749","DOIUrl":"https://doi.org/10.22236/jbes/9749","url":null,"abstract":"Background: Air pollution is a threat to the environment. Sources of air pollutants in urban environments can be in the form of dust, heavy metals, and hydrocarbons. Plants can help clean air pollutants from the atmosphere by absorption through the stomata, accumulating them, or by adsorption on the leaf surface. The Air Pollution Tolerance Index (APTI) is used as an evaluation benchmark for the Physiological conditions of plants exposed to air pollution. This research aims to study the physiological conditions of six tree species in air conditions in the Depok City area and to assess the plants' tolerance level based on the APTI calculation. Methods: The physiological parameters measured to calculate APTI were ascorbic acid, total chlorophyll, leaf extract pH, and Relative Water Content (RWC). The six tree species used as objects in this study were Artocarpus altilis, Artocarpus heterophyllus, Bauhinia purpurea, Ficus septica, Filicium decipiens, and Nephelium lappaceum. Results: Differences in the physiological conditions of six tree species in the Depok City area based on the average ascorbic acid values, total chlorophyll, leaf extract pH, and RWC. In addition, there are also differences in APTI parameters between the two research locations. Conclusions: Filicium decipiens is the plant species with the highest APTI, while Artocarpus heterophyllus has the lowest. Based on the APTI scores, Bauhinia purpurea, Ficus septica, Filicium decipiens, Nephelium lappaceum belong to moderately tolerant category, Artocarpus altilis belongs to an intermediate category, Artocarpus heterophyllus belongs to sensitive category.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47247790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-08-24DOI: 10.22236/jbes/11885
Siska Andriyani, Suparti
{"title":"The Effect of Lamtoro Leaf POC and Tofu Liquid Waste on the Growth of Cayenne Chili (Capsicum frutescens)","authors":"Siska Andriyani, Suparti","doi":"10.22236/jbes/11885","DOIUrl":"https://doi.org/10.22236/jbes/11885","url":null,"abstract":"Background: Liquid organic fertilizer is a fermented solution of organic plant residues, animal and human feces containing nutrients. Lamtoro and tofu liquid waste contain N, P, K, Mg, and Ca, which are suspected to have the potential for liquid organic fertilizer to increase the growth of cayenne pepper (C. frutescens). This study aims to determine the effect of giving POC from lamtoro leaves and tofu liquid waste on the growth of cayenne pepper (C. frutescens). Methods: This study used a completely randomized design (CRD) with two replications. POC volume 1:1 consisted of two-liter lamtoro leaf extract and 2-liter tofu liquid waste. The first factor is the volume concentration of POC with the respective concentrations of V0: 0%, V1: 10%, V2: 20%, and V3: 30%. The second factor is the time of spraying POC T1: 3 days interval and T2: 6 days interval. Parameters measured included plant height (cm), number of leaves (strands), and plant fresh weight (g). Results: 20% volume (V2) with three-day intervals (T1) gave the best results for plant height (20.10 cm), number of leaves (7.00 leaves), and plant fresh weight (11.50 g). 0% volume (V0) with an interval of 6 days (T2) gave the lowest yield on plant height (6.35 cm), number of leaves (3.00 leaves), and fresh weight of plants (3.00 leaves). Conclusions: Application POC from lamtoro leaves and tofu liquid waste can potentially increase the growth of cayenne pepper (C. frutescens).","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47682419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-05-03DOI: 10.22236/jbes/7111429
Bunga Indri Pertiwi, Isti Rusdiyani, Lukman Nulhakim
{"title":"Development of Animated Video Learning Media Using the Powtoon Application on the Theme of Caring for Living Things","authors":"Bunga Indri Pertiwi, Isti Rusdiyani, Lukman Nulhakim","doi":"10.22236/jbes/7111429","DOIUrl":"https://doi.org/10.22236/jbes/7111429","url":null,"abstract":"Background: The background of this research is (1) the lack of availability of learning media (2) the lack of student motivation to participate in the learning process; and (3) Science learning outcomes are still not optimal. This study aims to develop learning media, analyze feasibility, analyze effectiveness, and analyze students' responses to learning media using animated videos Powtoon on caring for living beings. Methods: Research and Development (R&D) 4-D approach. The final findings in this research and development are limited student trials of products in the form of animated video learning media using applications Powtoon for students of class IV Public Elementary School Cibeber District. Results: Effectiveness trials at the development stage were carried out by researchers by doing pre-test and post-test. The known value pre-test obtains an average value of learning outcomes of 55.68. As for value, the post-test received an average score of 79.37 learning outcomes, with the number of respondents or students used as test subjects as many as 80 students from four schools. In the subsequent trial, the researcher distributed questionnaires to teachers and students. This questionnaire is limited to knowing the responses and responses of students at the dissemination stage of using animated video learning media Powtoon by getting the results of the average percentage of teacher and student responses to enthusiastic video learning media, which reached 94% in the \"excellent\" category. Conclusions: Thus, using the animated video learning media Powtoon developed is suitable for the learning process.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45934165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pengaruh Abu Dasar Batubara dan Media Tanah Kompos terhadap Pertumbuhan Tanaman Cabe Merah (Capsicum annuum)","authors":"N. Elawati, Carmelia Santania Fahik, Arifah Nurul Izza, Fika Ainur Rahmawati","doi":"10.22236/jbes/7111185","DOIUrl":"https://doi.org/10.22236/jbes/7111185","url":null,"abstract":"Background: Kegiatan pembakaran batu bara di PLTU setiap tahunnya menyisakan limbah dalam jumlah besar yang dapat menjadi masalah pencemaran lingkungan. Abu dasar diketahui mengandung unsur hara yang dibutuhkan tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan campuran kompos sebagai amelioran pada bottom ash terhadap pH dan pertumbuhan tanaman Capsicum annuum. Metode: Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 3 kali ulangan dan 4 formulasi yaitu B0= limbah batubara (200g); B1 = 100g abu dasar : 100g media tanam; B2 = 50g abu dasar : 150g media tanam, dan B3 = 150g abu dasar : 50 g media tanam. Parameter yang diamati meliputi tinggi tanaman, jumlah daun, panjang akar, jumlah akar, biomassa total dan pH. Hasil: Hasil penelitian menunjukkan bahwa perlakuan B0 memiliki pH tertinggi dan B2 memiliki pH terendah. Perlakuan B2 berturut-turut menunjukkan tinggi tanaman tertinggi, biomassa total terbesar, dan panjang akar tertinggi, serta berpengaruh nyata terhadap jumlah daun, tetapi tidak berpengaruh nyata terhadap jumlah akar. Kesimpulan: Berdasarkan hasil penelitian menunjukkan bahwa aplikasi abu dasar dengan media kompos tanah berpengaruh terhadap pH dan pertumbuhan tanaman C. annuum terutama pada tinggi tanaman, jumlah daun, biomassa total, dan panjang akar. tanaman C. annuum","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42506775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-04-30DOI: 10.22236/jbes/7111357
Devi Bunga Pagalla
{"title":"Stages of Microspore Development in Eggplant (Solanum melongena L.)","authors":"Devi Bunga Pagalla","doi":"10.22236/jbes/7111357","DOIUrl":"https://doi.org/10.22236/jbes/7111357","url":null,"abstract":"Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45562171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-04-30DOI: 10.22236/jbes/7110797
Azrini Khaerah, None Ardianto
{"title":"Organoleptic Test of Kombucha Made from Various Tea Sources and Fermentation Time","authors":"Azrini Khaerah, None Ardianto","doi":"10.22236/jbes/7110797","DOIUrl":"https://doi.org/10.22236/jbes/7110797","url":null,"abstract":"Background: This research aims to find the best mixture of kombucha that can accept by the community in BTN Pa’jukukang, Bantaeng district. Kombucha is a fermented beverage made from tea and sugar mixed with the kombucha culture. A kombucha culture is a mixture of bacteria and yeast designated as SCOBY in this work. Methods: In this research, kombucha was made from 4 various teas, i.e., black tea, green tea, oolong tea, and white tea. Each tea was fermented for 7 and 14 days. The kombucha tea samples were subjected to an organoleptic test based on the consumer's acceptance level. The organoleptic test data were analyzed using the hedonic and score test. Results: The test result showed that respondents preferred to choose the color, the smell, and the taste of the black kombucha tea which was fermented for 7 days. Conclusions: Furthermore, the observation of the physical characteristics of the tea showed that the longer the fermentation time, the more acidic the kombucha. This acidic kombucha resulted from the decreasing sugar level during the fermentation process due to the activity of bacteria and khamir in the SCOBY.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135756770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-04-30DOI: 10.22236/jbes/719138
Polikarpia Wilhelmina Bani, Maria Delia Fuka Seran, Emilia Julyanti Bria
{"title":"Viabilitas Benih Jagung Lokal (Zea mays L.) Kabupaten Timor Tengah Utara Yang Diberi Perlakuan Kolkisin","authors":"Polikarpia Wilhelmina Bani, Maria Delia Fuka Seran, Emilia Julyanti Bria","doi":"10.22236/jbes/719138","DOIUrl":"https://doi.org/10.22236/jbes/719138","url":null,"abstract":"Background: Colchicine is a chemical mutagen that has been widely used to increase the viability of a plant. Most of the people of the North Central Timor (TTU) Regency still depend on local agricultural products, so one of the crops cultivated during the rainy season is local corn. This study aimed to determine the viability of local maize seeds in North Central Timor Regency and the appropriate concentration of colchicine to increase local maize germination. Methods: used in this research is an experimental method that gives colchicine treatment to 3 local corn varieties. The colchicine used was 0% (control), 40%, and 60%. The viability parameters observed were maximum growth potential (PTM), germination capacity (DB), and vigor index (IV). Results: showed that there were variations in the measurement of viability parameters observed both at maximum growth potential (PTM JBa 16,6%, JBu 35%, JKa 10%, JKu 8,33%), germination (DB 70%, JBu 81,66%, JKa 60%, JKu 61,66%) and vigor index (IV 68,33%, JBu 78,33%, JKa 63,33%, JKu 68,33%). Conclusions: the appropriate concentrations of colchicine to increase local corn germination were 40% and 60%, respectively. This can be seen in the viability parameters that show differences, namely the maximum growth potential (PTM) of flower corn with a colchicine concentration of 40%, which is 35%, the germination capacity (DB) of flower corn with a colchicine concentration of 60%, which is 90%, and the vigor index (IV) of flower corn. The concentration of colchicine 60% is 90%.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46872800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioedusciencePub Date : 2023-04-30DOI: 10.22236/jbes/7111234
Hastangka Hastangka, Latifa Nuraini
{"title":"Biological Education in Indonesian Senior High School: Study in Textbook and Curriculum Policy","authors":"Hastangka Hastangka, Latifa Nuraini","doi":"10.22236/jbes/7111234","DOIUrl":"https://doi.org/10.22236/jbes/7111234","url":null,"abstract":"Background: Biology education has a vital role in scientific development, strengthening the position of biology education for sustainable development and advancing biological science. During this time, biology materials developed rapidly. Methods: Writing the word Abstract using Cambria 9pt, in italics. The Abstract consists of 150-250 words written in one paragraph, containing the essence of the manuscript, background, research objectives, methods, results, and conclusions. Results: Biology education material that has developed since there were three changes to the curriculum in Indonesia after the issuance of Law Number 20 of 2003 concerning the National Education System does not appear to have shown any significant changes specifically in the subject matter for class X SMA. Curriculum policies and the development of textbooks in Indonesia focus more on and continue the material in the previous curriculum since the emergence of the competency-based curriculum (2004). Conclusions: The development of biology education in Indonesia from materials, textbooks, and curriculum policies does not show a significant change and development in the development of biology science for class X SMA. This study concludes that textbooks and curriculum policies have not been able to change the perspective and substance of the direction of learning biology in Indonesia. So the portrait of the capacity and insight of the Indonesian generation's knowledge about biology can be seen from the curriculum policies and the existing materials.","PeriodicalId":33216,"journal":{"name":"Bioeduscience","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48333059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}